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Effects of physicochemical factors on development and survival of Opisthorchis viverrini uterine eggs

Asian Pacific Journal of Tropical Biomedicine 2018年第9期8卷 431-435页

作者： Chanisala Sereewong Monticha Chaiyasaeng Naiyana Senasri Jukkrid Chaiyos Smarn Tesana Food-borne Parasite Research Group Department of Parasitology Faculty of Medicine Khon Kaen University Department of Fisheries Faculty of Natural Resources Rajamangala University of Technology Isan Sakonnakhon Campus

Objective: To investigate the maturity development of miracidia in uterine eggs from four portions of the Opisthorchis viverrini uterus and environmental factors possibly affected in maturation and infectivity of distal part uterine ***: Uteri of adult worms were divided into 4 equal *** of eggs was determined under light *** embryonated eggs were used to evaluate the effects of physicochemical factors: temperature, salinity, acidity, ultraviolet A, B, *** success was evaluated by feeding treated eggs to intermediate host snails and determining by using a PCR ***: eggs obtained from the uterus closest to the ovary(regions 1 and 2) failed to develop in *** from region 4 of the uterus(close to the genital pore) were used to study effects of physicochemical *** highest survival and infection success was in groups of eggs kept at 30 ℃(95.20%).The calculated period of loss infection success(LI_(50) and LI_(95)) on miracidia in distal uterine eggs by exposure to UV-A, UV-B and UV-C were 73 and 1 523 d; 8 and 20 d; 1 and 2 d, *** concentrations(LC_(50) and LC_(95)) of salinity, HCl and NaOH on miracidia in distal uterine eggs were 45.43 and 120.09 ppt, 0.01 and 0.25 M; 0.01 and 0.11 M, respectively, after 24 h ***: Opisthorchis viverrini eggs display a high tolerance to environmental conditions, especially after snail host eating for infection.

关键词： Opisthorchis viverrini Physicochemical Development Survival uterine eggs

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