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Predicting urine culture results in candidates for lithotripsy

Current Urology 2023年第2期17卷 113-117页

作者： Ofer N.Gofrit Roey Abudi Amitay Lorber Mordechai Duvdevani Faculty of Medicine Hebrew University of JerusalemJerusalemIsrael Department of Urology Hadassah University HospitalJerusalem91120Israel

Background:Urological guidelines assert that"urine culture should be obtained"before surgical management of ureteral or kidney ***,many surgeries are delayed by 1-3 days until the results of urine culture are *** this time,the patient frequently experience pain and possible kidney *** investigated the hypothesis that it is possible to predict the results of urine culture in candidates for surgical intervention using parameters that are accessible immediately upon *** and methods:A database of 1000 patients who underwent either percutaneous nephrolithotomy(PCNL)or ureteroscopy/retrograde intrarenal surgery was *** parameters potentially related to urinary infections and accessible to the clinician at the emergency department were correlated with the preoperative urine culture ***:Of the patients,234(23.4%)had positive *** multivariate analysis,only sex,hydronephrosis grade,and history of previous nephrolithotomy were significantly associated with a positive preoperative urine *** risk of a positive culture can be easily determined from a simple table or an Excel-based *** risk could be as low as 0.45%for a man without a history of PCNL and no hydronephrosis(4%in a woman with similar parameters)or as high as 79.5%in a man with a history of PCNL and hydronephrosis(85%in a woman with similar parameters).Conclusions:The risk of preoperative positive urine culture can be predicted using 3 parameters that are accessible upon *** low-risk cases,prompt surgical treatment can be provided,eliminating the anticipation time for urine culture results.

关键词： Ureteral calculi Renal calculi urine culture Ureteroscopy Percutaneous nephrolithotomy

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Prevalence and Antibiotic Resistance of Urinary Tract Pathogens, with Molecular Identification of Klebsiella pneumoniae, Klebsiella oxytoca, and Acinetobacter spp., Using Multiplex Real-Time PCR

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American Journal of Molecular Biology 2024年第4期14卷 245-260页

作者： Hawa Tarnagda Djénéba Ouermi Tani Sagna Wendyam Marie Christelle Nadembega Abdoul Karim Ouattara Lassina Traoré Rogomenoma Alice Ouedraogo Prosper Bado Bapio Valérie Elvira Jean Télesphore Bazie Nicole Bouda/Zongo Luc Zongo Albert Théophane Yonli Théodora Mahoukèdè Zohoncon Florencia Wendkuuni Djigma Jacques Simpore Centre de Recherche Biomolculaire Pietro Annigoni (CERBA) Ouagadougou Burkina Faso University Saint Thomas dAquin (USTA) Ouagadougou Burkina Faso Laboratoire de Biologie Molculaire et de Gntique (LABIOGENE) Universit Joseph KI-ZERBO Ouagadougou Burkina Faso Institute for Research in Applied Sciences (IRSS/CNRST) Ouaga Burkina Faso Universit Norbert Zongo/Centre Universitaire de Manga Avenue Maurice Yameogo Koudougou Burkina Faso Universit Nazi BONI Bobo-Dioulasso Burkina Faso Hpital Saint Camille de Ouagadougou (HOSCO) Ouagadougou Burkina Faso

Urinary tract infections (UTIs) caused by uropathogens are a significant public health problem, and their treatment primarily relies on antibiotic therapy. However, the increasing global development of antibiotic resistance necessitates updating diagnostic techniques to ensure higher sensitivity and specificity, especially with advancements in science and medicine. This study aimed to evaluate the prevalence of UTIs and antibiotic resistance profiles through urine culture, as well as to identify Klebsiella pneumoniae, Klebsiella oxytoca, and Acinetobacter spp. in urine samples using a molecular approach with multiplex real-time PCR. From May 3 to July 25, 2023, at the Pietro Annigoni Biomolecular Research Center (CERBA) and Saint Camille Hospital of Ouagadougou (HOSCO), 209 urine samples collected from patients with suspected UTIs were analyzed using both urine culture and multiplex real-time PCR. Among the 209 patients, 52.15% were male and 47.85% female, with an average age of 46.87 ± 21.33 years. urine cultures revealed an overall UTI prevalence of 23.44%, with a prevalence of 8.13% in men versus 15.31% in women (P = 0.023). The bacterial prevalence rates were as follows: Escherichia coli (12.92%), Klebsiella spp. (7.18%), Enterobacter cloacae (1.44%), Staphylococcus aureus (0.96%), and other bacteria. Klebsiella spp. demonstrated 100% resistance to Amoxicillin and Amoxicillin/Clavulanic Acid, while Escherichia coli showed 96.2% and 65.4% resistance to Amoxicillin and Amoxicillin/Clavulanic Acid, respectively. PCR analysis of the target bacteria revealed mono-infection prevalence rates of Klebsiella pneumoniae (10.39%), Klebsiella oxytoca (7.79%), and Acinetobacter spp. (7.79%), along with a co-infection prevalence rate of Klebsiella pneumoniae/Acinetobacter spp. (1.30%). This study demonstrated that PCR, with its high sensitivity and specificity, could effectively distinguish Klebsiella pneumoniae from Klebsiella oxytoca and detect Acinetobacter spp. in less tha

关键词： Urinary Tract Infections Klebsiella pneumoniae Klebsiella oxytoca Acinetobacter spp. urine culture Real-Time PCR

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Analysis on clinical distribution and drug resistance of pathogenic bacteria in the of 1368 cases mid-stream urine

Analysis on clinical distribution and drug resistance of pat...

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2014浙江省医学会男科学泌尿外科学学术年会

作者： Xiang HONG Qing kang XU Yue DUAN Wei hua SHEN Tain qing YU Xian jun SUN Department of Urology Zhejiang Provincal Crops Hospital of Chinese People’s Armed Police Force

Objective to analyze the pathogens and antibiltic resistance of urine cultu-re in patients with ureteral stones for the guidance of clinical medicine treatment and prevention and rational use of *** 1368 patients of ureteral stones treated clean urine culture and sensitivity from January of 2011-June of 2013 in our *** 238 pathogens were isolated from 1368 urine *** positive cocci accounted for 30.25%(72 cases),The majority was Enterococcus fasium and Staphylococcus aureus;In which Gram negative bacilli accounted for 66.81%(159 cases),the majority was the Escherichia coli(***)and Pseudomonas aeruginosa;Fungi accounted for 2.94%(7cases).There are a large difference in resistance to different bacterials,the performance is the different degrees of *** This study showed that with positive rate of 17.4%of the *** majority was Enterococcus fasium and the Escherichia coli(***).It is necessary for patients with ureteral calculi gived urine culture before *** are lagare significance to reduce the generation of broad-spectrum antibiotics and a reasonable choice of antibiotics.

关键词： Ureteral stones urine culture pathogens drug resistance

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Risk factors for bladder calculi in patients with benign prostatic hyperplasia

Risk factors for bladder calculi in patients with benign pro...

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第十二次全国中西医结合男科学术大会暨全国中西医结合男科诊疗技术研修班暨2017上海市中西医结合学会上海市中医药学会泌尿男科专业委员会学术年会

作者： Xiao-Dong Jin Wei Huang Min Cao Jun-Jie Cao Hong-Shen Wu Yong-Yi Yang Zi-Meng Xu Department of Urology The First Affiliated HospitalZhejiang University School of Medicine

Background and objective:Bladder calculi is not only a common complication of benign prostatic hyperplasia(BPH) but also an absolute indication for surgical therapy of patients with *** aim to investigate the risk factors for bladder calculi in patients with BPH and to reduce the surgical intervention related to bladder *** and Methods:Between January 2015 and October 2016,332 patients with BPH underwent surgical therapy were retrospectively *** with BPH were categorized into two groups:94 patients with bladder calculi in group 1 and 238 patients without bladder calculi in group *** history,age,body mass index(BMI),total prostate specific antigen(PSA),total prostate volume(TPV),International Prostate Symptom Score(IPSS),intravesical prostatic protrusion(IPP),urodynamic parameters and urine culture were compared between ***:There was no significant difference in the age,BMI,peak flow rate or IPSS between ***,total PSA and duration of BPH were significantly lower in group 1 than those in group *** addition,IPP was significantly higher in group 1 than group 2(purine culture than that of group 2(p=0.046).Multivariate analysis indicated that IPP was a significant independent risk factor for the presence of bladder ***:This study revealed that the preoperative positive urine culture and IPP maybe are risk factors for bladder calculi in patients with benign prostatic hyperplasia.

关键词： bladder calculi benign prostatic hyperplasia intravesical prostatic protrusion urine culture urinary retention

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