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Green tea polyphenol epigallocatechin-3-gallate inhibits the expression of nitric oxide synthase and generation of nitric oxide induced by ultraviolet B in HaCaT cells

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Chinese Medical Journal 2006年第4期119卷 282-287页

作者： SONG Xiu-zu BI Zhi-gang XU Ai-e Department of Dermatology Third Hospital of Hangzhou Hangzhou 310009 China Department of Dermatology First Affiliated Hospital of Nanjing Medical University Nanjing 210029 China

Background Nitic oxide （NO） has been implicated in the pathogenesis of various inflammatory diseases, including sunburn and pigmentation induced by ultraviolet irradiation. Epigallocatechin-3-gallate （EGCG） is the major effective component in green tea and can protect skin from ultraviolet-induced damage. The purpose of this study was to investigate the protective mechanisms of EGCG on inducible nitric oxide synthase （iNOS） expression and NO generation by ultraviolet B （UVB） irradiation in HaCaT cells. Methods HaCaT cells were irradiated with UVB 30 mJ/cm^2 and pretreated with EGCG at varying concentrations. The iNOS mRNA was detected by reverse transcriptase polymerase chain reaction （RT-PCR） and NO production was quantified by spectrophotometric method. The expression of NF-κB P65 was measured by immunofluorescence cytochemistry staining. Results The expression of iNOS mRNA and generation of NO in HaCaT cells were increased by UVB irradiation. EGCG down regulated the UVB-induced iNOS mRNA synthesis and NO generation in a dose dependent manner. The UVB-induced activation and translocation of NF-κB were also down regulated by EGCG treatment in HaCaT cells （P〈0.01）. Conclusions Green tea derived-EGCG can inhibit and down regulate the UVB-induced activation and translocation of NF-κB, expression of iNOS mRNA and generation of NO respectively, indicating EGCG may play a protective role from UVB-induced skin damage.

关键词： epigallocatechin-3-gallate ultraviolet rays keratinocytes inducible nitric oxide synthase

The Association of Socioeconomic Status with the Burden of Cataract-related Blindness and the Effect of ultraviolet Radiation Exposure: An Ecological Study

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Biomedical and Environmental Sciences 2021年第2期34卷 101-109页

作者： DENG Yan YANG Dan YU Jia Ming XU Jing Xian HUA Hui CHEN Ren Tong WANG Nan OU Feng Rong LIU Ru Xi WU Bo LIU Yang Department of Environmental Health School of Public HealthChina Medical UniversityShenyang 110122LiaoningChina Department of Ophthalmology the Fourth Affiliated HospitalChina Medical UniversityShenyang 110032LiaoningChina Academic Affairs Office China Medical UniversityShenyang 110122LiaoningChina Department of Immunology and Rheumatology First HospitalChina Medical UniversityShenyang 110001LiaoningChina Department of Anal and Rectal Diseases First HospitalChina Medical UniversityShenyang 110001LiaoningChina

Objective To assess the association of socioeconomic status with the burden of cataract blindness in terms of year lived with disability(YLD) rates and to determine whether ultraviolet radiation(UVR) levels modify the effect of socioeconomic status on this health burden.Methods National and subnational age-standardized YLD rates associated with cataract-related blindness were derived from the Global Burden of Disease(GBD) study 2017. The human development index(HDI) from the Human Development Report was used as a measure of socioeconomic status.Estimated ground-level UVR exposure was obtained from the Ozone Monitoring Instrument(OMI)dataset of the National Aeronautics and Space Administration(NASA).Results Across 185 countries, socioeconomic status was inversely associated with the burden of cataract blindness. Countries with a very high HDI had an 84% lower age-standardized YLD rate [95%confidence interval(CI): 60%–93%, P < 0.001] than countries with a low HDI;for high-HDI countries, the proportion was 76%(95% CI: 53%–88%, P < 0.001), and for medium-HDI countries, the proportion was48%(95% CI: 15%–68%, P = 0.010;P for trend < 0.001). The interaction analysis showed that UVR exposure played an interactive role in the association between socioeconomic status and cataract blindness burden(P value for interaction = 0.047).Conclusion Long-term high-UVR exposure amplifies the association of poor socioeconomic status with the burden of cataract-related blindness. The findings emphasize the need for strengthening UVR exposure protection interventions in developing countries with high-UVR exposure.

关键词： Cataract Blindness Socioeconomic status ultraviolet rays Global burden of disease

Curcumin from Jianghuang (Rhizoma Curcumae Longae) protects against exposure to ultraviolet B by antioxidation and attenuating mitochondrion-dependent apoptosis

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Journal of Traditional Chinese Medicine 2020年第5期40卷 782-791页

作者： Hou Jiguang Fang Fang Kang Shunai Wang Zhicheng Yang Yanming Department of Radiotherapy Second Hospital of Jilin UniversityChangchun 130041China NHC Key laboratory of Radiobiology(Jilin University) School of Public HealthJilin UniversityChangchun 130021China

OBJECTIVE:To investigate the protective effect of curcumin extracted from Jianghuang(Rhizoma Curcumae Longae)against ultraviolet B(UVB)and the possible mechanism.METHODS:Effects of curcumin were detected in vivo and in vitro.Morphological changes of white guinea pig skin were assessed by hematoxylin and eosin staining.Ha CaT cell proliferation was measured by 3-[4,5-dimethylthylthiazol-2-yl]-2,5 diphenyltetrazolium broide(MTT)assays.The cell cycle distribution,apoptotic rate,level of reactive oxygen species(ROS),mitochondrial membrane potential,and intracellullar calcium ion concentration of Ha CaT cells were detected by flow cytometry.Antioxidant levels in skin tissues and Ha Cat cells were measured by biochemical methods.RESULTS:UVB inhibited in vitro cell proliferation by inducing G2/M arrest,increasing ROS,apoptosis,and necrosis,and decreasing B-cell lymphoma-2,and increasing Bax,cytochrome c,and caspase-3 levels.CONCLUSION:Curcumin blocks the effects of UVB by reducing ROS and apoptosis,and reversing UVB-induced changes in the expression of apoptotic proteins.The mitochondrial pathway is involved in curcumin-regulated apoptosis.

关键词： ultraviolet rays Curcumin Antioxidants Apoptosis Mitochondria

Regulation of Eaf2 in mouse lens cells apoptosis induced by ultraviolet radiation

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International Journal of Ophthalmology(English edition) 2012年第5期5卷 570-575页

作者： Fan Xiao Jin-Song Zhang Jiang-Yue Zhao and Di Wu Department of Ophthalmology the Fourth Affiliated Hospital of China Medical University Shenyang 110005 Liaoning Province China

AIM: To investigate the regulation of Eaf2 protein in mouse lens cells apoptosis induced by ultraviolet (UV) radiation. METHODS: An eye of Eaf2 gene knockout mice or normal control mice was exposed to UV radiation, and the other one was non-exposed. All of lenses were analyzed by TUNEL and caspase 3 activity assays to determine the difference of the apoptosis induced by UV radiation. In addition, exposed and non-exposed lenses were analyzed by quantified p53 expression and real-time reverse transcription-polymerase chain reaction (RT-PCR) of Bax, Bid, Apaf-1, Puma and Noxa, to compare Eaf2 gene knockout mice and normal control mice. RESULTS: UV radiation caused apoptosis of lens cells in normal control mice and Eaf2 knockout mice. Activity of caspase 3 was significantly higher in normal control mice than Eaf2 knockout mice. Expression of p53 protein was significantly higher in lenses exposed to UV radiation than nonexposed lenses, but was similar between Eaf2 gene knockout mice and normal control mice in the same UV condition. After exposing to UV radiation, the analysis of real-time RT-PCR demonstrated that mRNA levels of Puma and Noxa were significantly higher in lenses of normal control mice than Eaf2 gene knockout mice, and that mRNA levels of Bax, Bid and Apaf-1 were not significantly different between gene knockout mice and normal control mice. CONCLUSION: Eaf2 increases lens cells apoptosis induced by ultraviolet radiation. And Eaf2 up-regulates expression of the Puma and the Noxa to act on lens cells apoptosis after UV radiation.

关键词： Eaf2 p53 apoptosis Noxa Puma ultraviolet rays

APP17肽通过抑制细胞内ROS保护紫外线照射后人皮肤成纤维细胞(英文)

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中华临床医师杂志（电子版） 2011年第21期5卷 6322-6328页

作者：陈慧连石朱威 Department of Dermatology and Venereology Xuanwu Hospital of the Capital Medical University

Objective ultraviolet light (UV) is known to cause photoaging of skin.UV irradiation can damage proliferation capacity and induce collagenase in fibroblasts in the dermis.Many researchers have explored the potential photo-protective agents;however,no ideal agent has been widely accepted.Amyloid precursor protein 17-mer peptide (APP17-mer peptide),an active peptide segment,has been reported to be responsible for the trophic effect in clonal CNS neuronal line,fibroblast cell line and HaCat cells.The aim of this study was to explore the effects of APP17-mer peptide on cultured fibroblasts after ultraviolet irradiation.Methods Human skin fibroblasts were cultured in DMEM medium with or without APP17-mer peptide (concentrations ranging from 20μmol/L,40 μmol/L,to 80μmol/L).The cultured fibroblasts were exposed to a single UV irradiation,and the proliferation activity of fibroblasts was detected by a MTT assay.The expression of matrix metalloproteinase-1 (MMP-1) mRNA was analyzed quantitatively following real-time RT-PCR.The generation of intracellular reactive oxygen species (ROS) was measured with fluorescent quantitation method.Results A single exposure to UV irradiation depressed proliferation activity of fibroblasts compared with sham-irradiated control (P0.05).A single exposure of fibroblasts to UV irradiation resulted in 1.78 fold up-regulation of MMP-1 mRNA compared with unirradiated sample (P<0.05),and 40μmol/L and 80μmol/L APP17-mer peptide decreased the expression of MMP-1 mRNA (P<0.05 and P<0.01,respectively).UV irradiation increased generation of ROS in cultured fibroblasts (P<0.05).40μmol/L APP17-mer peptide inhibited the generation of ROS in irradiated fibroblasts.Conclusions APP17-mer peptide can enhance proliferation activity of fibroblasts after exposure to UV irradiation;it can also inhibit MMP-1 mRN

关键词： Amyloid protein precursor ultraviolet rays Reactive oxygen species Matrix metalloproteinase 1

Study on Pattern Recognition of Radix Cynanchi Paniculati Based on ultraviolet Spectm

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Study on Pattern Recognition of Radix Cynanchi Paniculati Ba...

Guinea　Pig　Model　of　Cataract　induced　by　ultraviolet　Ray　Irradiation

2011 International Conference on Advanced Materials and Information Technology Processing(AMITP 2011)

作者： Jinhong Gao Chemistry and Engineering Department Weinan Teachers College

This paper analyzes the data acquires from ultraviolet spectroscopy. Combining with information theory, it conducts pattern recognition analysis research to Radix Cynanchi Paniculati medicinal materia

ISBN: (纸本)9783037851555

关键词： Radix Cynanchi Paniculati ultraviolet spectrum pattern recognition Base of a number system Information Theory ultraviolet visible spectroscopy Root ultraviolet spectroscopy Pattern recognition Radix ultraviolet rays

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The Journal of Biomedical Research 1994年第1期18卷 48-48页

作者： Zhu Zhi,et al.ACTA ACADEMIAE MEDICINAE NANJING,1994,14(1):35-36

Guinea pig model of cataract was obtained by ultraviolet ray irradiation on both eyes of 47 guinea pigs for 6-8 months. Seven eye-lenses irradiated by ultraviolet ray selected randomly for optical micro-scopic and electron microscopic examinations revealed typical pathological findings such as obvious swelling and widening of gaps of the lens fibres.

关键词： cataract ultraviolet rays guinea pig