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Survival of Human Metallothionein-2 Transplastomic Chlamydomonas reinhardtii to ultraviolet b Exposure

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Acta biochimica et biophysica Sinica 2006年第3期38卷 187-193页

作者： Yu-Kun ZHANG~1 Gui-Fang SHEN~2 bing-Gen RU~1*1 National Laboratory of Protein Engineering and Plant Genetic Engineering,Peking University,beijing 100871,China 2 biotechnology Research Institute,the Chinese Academy of Agricultural Sciences,beijing 100081,China National Laboratory of Protein Engineering and Plant Genetic Engineering Peking UniversityBeijing 100871 China biotechnology Research Institute the Chinese Academy of Agricultural SciencesBeijing 100081 China

Solar ultraviolet (UV) radiation has a great influence on green organisms,especially planktonlike Chlamydomonas.A human metallothionein-2 gene,which is generally considered to have an anti-radiationfunction by its coding product,was transferred into the chloroplast genome of Chlamydomonas reinhardtii.To dynamically measure the UV effects on Chlamydomonas cells grown in liquid tris-acetate-phosphatemedium,a new method was developed based on the relationship between the chlorophyll content of an algalculture and its absorbance at 570 nm after the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromideassay.In this experiment,both the wild-type and the transplastomic C.reinhardtii ceils were cultivated in 96-well microplates containing liquid tris-acetate-phosphate medium in the absence or presence of zinc,copper,cadmium and cysteine.The transgenic C.reinhardtii showed a higher resistance than wild-type to UV-bexposure under all the examined conditions.Metals in the medium had positive impacts on both types of cells,but had significant influence only on the transplastomic cells.However,the high cell viability of the trans-genie alga at the end of the 8 h UV-b treatment disappeared after a 20-h recovery culture.Cysteine did notprotect cells from UV-b damage,but clearly enhanced the growth of both wild-type and transgenic C.reinhardtii.

关键词： ultraviolet b MTT transplastomic Chlamydomonas metallothionein

Increased 5-hydroxymethylcytosine and Ten-eleven Translocation Protein Expression in ultraviolet b-irradiated HaCaT Cells

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Chinese Medical Journal 2017年第5期130卷 594-599页

作者： Dan Wang Jin-Hua Huang Qing-Hai Zeng Can Gu Shu Ding Jian-Yun Lu Jing Chen Sheng-bo Yang Department of Dermatology Third Xiangya Hospital Central South University Changsha Hunan 410013 China Department of Community Nursing Xiangya School of Nursing Central South University Changsha Hunan 410013 China

background： DNA hydroxymethylation refers to a chemical modification process in which 5-methylcytosine （5mC） is catalyzed to 5-hydroxymethylcytosine （5hmC） by ten-eleven translocation （TET） family proteins. Recent studies have revealed that aberrant TETs expression or 5hmC level may play important roles in the occurrence and development of various pathological and physiological processes including cancer and aging. This study aimed to explore the relation between aberrant DNA hydroxymethylation with skin photoaging and to investigate the levels of TETs, 5mC, and 5hmC expression 24 h after 40 mJ/cm^2 and 80 mJ/cm^2 doses of ultraviolet b （UVb） irradiation to HaCaT cells. Methods： To explore whether aberrant DNA hydroxymethylation is also related to skin photoaging, 40 mJ/cm^2 and 80 mJ/cm^2 doses of UVb were chosen to treat keratinocytes （HaCaT cells）. After 24 h of UVb irradiation, 5mC and 5hmC levels were determined by immunohistochemistry （IHC） and immunofluorescence （IF）, and at the same time, the expression levels of matrix metalloproteinase 1 （MMP-1） and TETs were assessed by reverse transcription-polymerase chain reaction or Western blot analysis. Results： After 40 mJ/cm^2 and 80 mJ/cm^2 doses of UVb exposure, both IHC and IF results showed that 5hmC levels increased significantly, while the 5mC levels did not exhibit significant changes in HaCaT cells, compared with HaCat cells without UVb exposure. Moreover, compared with HaCat cells without UVb exposure, the levels ofTET1, TET2, and TET3 mRNA and protein expression were significantly upregulated （mRNA： P = 0.0022 and 0.0043 for TET1; all P 〈 0.0001 for TET2; all P = 0.0006 for TET3; protein： P = 0.0012 and 0.0006 tbr TET 1 ; all P = 0.0022 for TET2; and all P = 0.0002 for TET3）, and the levels of MMP- 1 mRNA expression increased dose dependently in 40 mJ/cm^2 and 80 mJ/cm^2 UVb-irradiated groups. Conclusion： UVb radiation could cause increased 5hmC and TET expression, which might become a novel biomarker in UVb-related skin aging.

关键词： 5-hydroxymethylcytosine 5-methylcytosine DNA Hydroxymethylation Ten-eleven Translocation ultraviolet b

Receptor interacting protein 1 involved in ultraviolet b induced NIH3T3 cell apoptosis through expression of matrix metalloproteinases and reactive oxygen species production

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Chinese Medical Journal 2013年第22期126卷 4327-4333页

作者： YAN Yan LI Li XU Hao-xiang PENG Shi-guang, QU Tao WANG bao-xi Department of Dermatology Peking Union Medical College Hospital Chinese Academy of Medical Sciences & Peking Union Medical College Beijing 100730 China Department of Dermatology Beijing Chaoyang Hospital CapitalMedical University Beijing 100020 China Institute of Dermatology Chinese Academy of Medical Sciences andPeking Union Medical College Nanjing Jiangsu 210042 China

background Receptor interacting protein 1 （RIP1）, which plays a key role in apoptosis, cell survival and programmed cell necrosis, is one of the most important proteins in the RIP family. The purpose of this study was to investigate the roles of RIP1 in the apoptosis, the generation of reactive oxygen species （ROS） and the expression of matrix metalloproteinases （MMPs） induced by ultraviolet b （UVb） in fibroblasts. Methods siRNA targeting RIP1 was used to silence RIP1 expression in the NIH3T3 fibroblasts. The mRNA and protein levels of MMP-1 and MMP-3, caspase-3 and -8 activities, and ROS activities were determined by reverse transcriptasequantitative polymerase chain reaction （RT-qPCR）, immunoblotting, caspase activity assay, immunofiuorescence, and flow cytometry. Results The mRNA and protein expressions of MMP-1 and MMP-3 were significantly increased in RIP1 deficient NIH3T3 cells at 24 hours after UVb treatment. At 24 hours after exposure to UVb, RIP1 deficient NIH3T3 cells presented apoptotic morphology, and the apoptosis rate was significantly increased accompanied by pronounced increase in caspase-8 and -3 activities. ROS production was inhibited by UVb at 12 hours in RIP1 deficient NIH3T3 cells. Conclusion RIP1 is involved in NIH3T3 cell damage induced by UVb via participating in the apoptosis, expression of MMPs and ROS production.

关键词： receptor interacting protein NIH3T3 ultraviolet b apoptosis matrix metalloproteinase

Hydrogen-rich saline protects against ultraviolet b radiation injury in rats

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The Journal of biomedical Research 2012年第5期26卷 365-371页

作者： Ze Guo bingrong Zhou Wei Li Xuejun Sun Dan Luo Department of Dermatology the First Affiliated Hospital of Nanjing Medical UniversityNanjingJiangsu 210029China Department of Diving Medicine Faculty of Naval MedicineSecond Military Medical UniversityShanghai 200433China

Exposure of skin to solar ultraviolet（UV） radiation induces photo-damage.ultraviolet b（UVb） is the major component of UV radiation which induces the production of reactive oxygen species（ROS） and plays an impor-tant role in photo-damage.Hydrogen gas reduces ROS and alleviates inflammation.In this study,we sought to demonstrate that hydrogen-rich saline has the effect on skin injuries caused by UVb radiation.UVb radiation was irradiated on female C57bL/6 rats to induce skin injury.Hydrogen-rich saline and nitrogen-rich saline were ad-ministered to rats by intraperitoneal injection.Skin damage was detected by microscope after injury.UVb radia-tion had a significant affection in tumor necrosis factor alpha,interleukin（IL）-1β and IL-6 levels,tissue superox-ide dismutase,malondialdehyde and nitric oxide activity.Hydrogen-rich saline had a protective effect by altering the levels of these markers and relieved morphological skin injury.Hydrogen-rich saline protected against UVb radiation injury,possibly by reducing inflammation and oxidative stress.

关键词： ultraviolet b photo-damage oxidative stress hydrogen-rich saline skin

Protective effect of sulfated polysaccharides from a Celluclast-assisted extract of Hizikia fusiforme against ultraviolet b-induced photoaging in vitro in human keratinocytes and in vivo in zebrafish

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Marine Life Science & Technology 2019年第1期1卷 104-111页

作者： Lei Wang Jae Young Oh Hye-Won Yan Hyun Soo Kim You-Jin Jeon Department of Marine Life Sciences Jeju National UniversityJeju CityJeju Self-Governing Province 63243Republic of Korea Marine Science Institute Jeju National UniversityJeju CityJeju Self-Governing Province 63333Republic of Korea

Our previous study evaluated the in vitro and in vivo antioxidant activities of sulfated polysaccharides from a Celluclastassistedextract of Hizikia fusiforme (HFPS). The results indicate that HFPS possesses potent antioxidant activity and suggestthe potential use of HFPS to combat photoaging. In this study, we investigated the ultraviolet (UV) protective effect of HFPSin vitro in keratinocytes (HaCaT cells) and in vivo in zebrafish. The results indicate that HFPS significantly reduced thelevel of intracellular reactive oxygen species (ROS) and improved the viability of UVb-irradiated HaCaT cells. In addition,HFPS remarkably decreased apoptosis formation in UVb-irradiated HaCaT cells in a dose-dependent manner. The in vivotest results also demonstrate that HFPS significantly reduced intracellular ROS levels, cell death, NO production, and lipidperoxidation levels in UVb-irradiated zebrafish in a dose-dependent manner. These results suggest that HFPS possessesstrong in vitro and in vivo UV-protective effects, making it a potential ingredient in the cosmeceutical industry.

关键词： Hizikia fusiforme Sulfated polysaccharides ultraviolet b

Deciphering skin re-pigmentation patterns in vitiligo:an update on the cellular and molecular events involved

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Chinese Medical Journal 2020年第10期133卷 1231-1238页

作者： Tie-Chi Lei Vincent JHearing departm ent of Dermatology Renmin Hospital of Wuhan UniversityWuhanHubei 430060China DASS Manuscript HaymarketVAUSA.

Current treatment of vitiligo is still a great challenge,since most cases of vitiligo have variable re-pigmentation outcomes due to their unpredictable responses to existing therapeutic regimens.There is an urgent need to identify this re-pigmentation process and to develop novel therapies.This review illustrates the most current research and latest understanding of vitiligo skin re-pigmentation and related regulatory mechanisms.Literature was collected from PubMed until January 2020,using the search terms including"vitiligo,""re-pigmentation,""phototherapy,""narrow-band ultraviolet b,""excimer,""fractional carbon dioxide laser,"and"melanocyte stem cells."Literature was mainly derived from English articles.Article type was not limited.Emerging evidence suggests that patients with vitiligo present various re-pigmentation patterns following ultraviolet b phototherapy,which relies on different cell reservoirs from the perilesional margins and/or from uninvolved hair follicles to replenish functional melanocytes that are lost in vitiliginous skin.The following events are likely to be involved in this re-pigmentation process,including:1)changes in the paracrine secretion and distribution of transforming growth factor-β1 in the bulge area and in the epidermis;2)the enhanced transfer of dermal pro-melanogenic growth factors to the epidermis;and 3)the induction of a C-X-C motif chemokine ligand(CXCL)12-enriched micro-environment that efficiently recruits CXCR4-or CXCR7-positive melanocytes.Ongoing studies on the cellular and molecular events underlying vitiligo re-pigmentation will help design new therapeutic strategies to improve treatment outcomes.

关键词： Vitiligo Re-pigmentation Melanocyte ultraviolet b Skin trauma