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DNA hybridization chain reaction and DNA supersandwich self-assembly for ultrasensitive detection

Science China Chemistry 2017年第3期60卷 311-318页

作者： Nannan Liu Fujian Huang Xiaoding Lou Fan Xia Key Laboratory of Carbon Materials of Zhejiang Province College of Chemistry & Materials EngineeringWenzhou UniversityWenzhou 325027China Hubei Key Laboratory of Bioinorganic Chemistry & Materia Medica School of Chemistry and Chemical EngineeringHuazhong University of Science and Technology CAS Key Laboratory of Soft Matter Chemistry Department of Polymer Science and EngineeringUniversity of Science and Technology of China

The fabrication of sensitive sensors with high selectivity is highly desirable for the detection of some important biomarkers,such as nucleic acids,proteins,small molecules and ions.DNA hybridization chain reaction(HCR) and DNA supersandwich self-assembly(SSA) are two prevalent enzyme-free signal amplification strategies to improve sensitivity of the sensors.In this review,we firstly describe the characteristics about DNA HCR and DNA SSA,and then summarize the advances in the one-dimensional DNA nanostructures assisted by HCR and SSA.This review has been divided into three parts according to the two signal amplification methods and highlights recent progress in these two strategies to improve the detection sensitivity of proteins,nucleic acids,small molecules and ions.

关键词： signal amplification method hybridization chain reaction supersandwich self-assembly ultrasensitive detection

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Conducting polymer engineered covalent organic framework as a novel electrochemical amplifier for ultrasensitive detection of acetaminophen

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Chinese Chemical Letters 2021年第6期32卷 2061-2065页

作者： Yao Xie Yuling Chen Xin Sun Yang Wang Yi Wang School of Chemistry and Chemical Engineering Yangzhou UniversityYangzhou 225002China Engineering Research Center of Clinical Functional Materials and Diagnosis&Treatment Devices of Zhejiang Province Wenzhou InstituteUniversity of Chinese Academy of SciencesWenzhou 325001China

Two-dimensional covalent organic framework(COF)has distinctive properties that offer potential opportunities for developing advanced electrode materials.In this work,a core-shell material composed of TAPB-DMTP-COF(TAPB,1,3,5-tris(4-aminophenyl)benzene;DMTP,2,5-dimethoxyterephaldehyde)core and conducting polymer shell,TAPB-DMTP-COF@PANI,was synthesized solvothermally using a polymerization method.The structural cha racteristics of the prepared composite were revealed by X-ray diffraction patterns(XRD),fourier transform infrared spectra(FTIR),X-ray photoelectron spectroscopy(XPS),transmission electron microscopy(TEM).The electrochemical analyses were verified by subsequent monitoring of trace levels of acetaminophen.This resultant composite not only facilitated acetaminophen to interact with absorption sites byπ-πstacking effect and hydrogen bonding but also overcame the poor conductivity of COF.Under the optimal conditions,a low limit of detection of 0.032μmol/L and wide linear range of 0.10-500μmol/L were obtained.The electrochemical platform was almost unaffected by other interfering substances,and successfully applied for the practical detection of acetaminophen in commercial tablet,human blood serum and urine.The enhanced performance makes this COF based core-shell composite a promising material in electrochemical senso r.

关键词： Covalent organic frameworks Conducting polymer Electrochemical sensor ultrasensitive detection Acetaminophen

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Asymmetric polymerase chain reaction and loop-mediated isothermal amplification(AP-LAMP) for ultrasensitive detection of microRNAs

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Chinese Chemical Letters 2020年第1期31卷 159-162页

作者： Ruili Wang Ling Lan Li Liu Liang Cheng Beijing National Laboratory for Molecular Sciences(BNLMS) CAS Key Laboratory of Molecular Recognition and FunctionCAS Research/Education Centre for Excellence in Molecular SciencesInstitute of ChemistryChinese Academy of SciencesBeijing 100190China University of Chinese Academy of Sciences Beijing 100049China

In this manuscript,we first report an ultrasensitive detection assay of microRNA by combing asymmetric polymerase chain reaction(A-PCR)and loop-mediated isothermal amplification(LAMP)technology.Using A-PCR obtained an extended single strand to form LAMP stem-loop structure under isothermal amplification conditions.We used miRNAs as a loop primer probe in LAMP reaction and completed its ultrasensitive and rapid detection.The established method furnished a fast,specific and efficient detection of target miRNA with a detection limit as low as 10 amol/L in 90 min.

关键词： microRNA LAMP Asymmetric PCR ultrasensitive detection Rapid detection

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Construction of Pillar[5]arene-Based Supramolecular Polymer Gel for ultrasensitive Multi-Analytes detection and Separation

Construction of Pillar[5]arene-Based Supramolecular Polymer ...

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中国化学会·第十五届全国有机合成化学学术研讨会

作者：张有明 Wei Zhu Hong Yao Tai-Bao Wei Qi Lin 西北师范大学 College of Chemistry and Chemical Engineering Northwest Normal University

In this article, we report a novel approach for the development of ultrasensitive multi-analytes detection and separation.First, we used a novel phthalimide-functionalized pillar[5]arene(PF) and a bis-bromohexane-functionalized pillar[5]arene(BF) to construct a linear supramolecular polymer(PFBF). Then, the obtained linear supramolecular polymer could form a stable two-dimensional supramolecular polymer gel(PFBF-G) in cyclohexanol with strong aggregation induced emission(AIE). Interestingly, the PFBF-G shows ultrasensitive fluorescent response for Fe, Hg and Ag in gel states, by rationally introduced Fe and Hg into the PFBF-G, the obtained metallogels(PFBF-FeG and PFBF-HgG) could ultrasensitive fluorescently sense Fand Br, respectively.More importantly, the xerogel of PFBF-G shows excellent separation properties for these metal ions and the absorption rates were up to 99.95%

关键词： ultrasensitive detection excellent separation supramolecular polymer gel pillararene

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Ultra-sensitive detection of macromolecules using signal amplification and ambient mass spectrometry

Ultra-sensitive detection of macromolecules using signal amp...

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2018年中国质谱学术大会（CMSC2018）

作者： Shuting Xu Yu Bai Huwei Liu Beijing National Laboratory for Molecular Sciences College of Chemistry and Molecular EngineeringPeking University

Protein biomarker detection are crucial in the clinical diagnosis 1. In recent years, Mass spectrometry(MS) has been wildly applied biomarker analysis either in proteomic aspect or target biomarker detection because of its low detection limits, large dynamic range and better spectral resolution. However, ultrasensitive direct detecting of large biomolecules, such as proteins and DNA etc., remains challenging. Signal amplification strategies using small molecules as mass reporters enable the higher sensitive detection of large molecules 2, 3. Different from conventional ionization technique, ambient ionization technique offers MS more possibilities in fast screening and detection. In our work, novel mass tag was designed and synthesized. Combining ambient ionization MS with the signal amplification strategy, the ultrasensitive detection method was established for DNA and protein biomarkers. Attomolar detection limit of target analytes were achieved through this approach, which showed great promise for large biomolecules detection in early disease diagnosis and single-cell analysis.

关键词： Signal amplification ambient MS ultrasensitive detection biomarker

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