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Comparative studies on histological and ultra-structure of the pituitary of different ploidy level fishes

Science China(Life Sciences) 2006年第5期49卷 446-453页

作者： LONG Yu LIU Shaojun HUANG Weiren ZHANG Jian SUN Yuandong ZHANG Chun CHEN Song LIU Jinghui LIU Yun College of Life Sciences Hunan Normal UniversityChangsha 410081China

The histological and ultra-structure of the pituitary in diploid red crucian carp(Carassius auratus red var.),triploid crucian carp and allotetraploid hybrids within and after the breeding season were comparatively *** result showed that there were six endocrine cell types in the pituitary of these three kinds of fishes,and there was an obvious difference in cell size among different ploidy level *** for the same type of pituitary cells,the cell size was increased gradually with the in-creasing ploidy *** the breeding season,the allotetraploid hybrids had higher proportion of go-nadotropin cells(GTH)than triploids,and the triploids had higher proportion of GTH than *** results were related to the earlier sexual maturity of allotetraploid hybrids and sterility of triploid cru-cian *** the other hand,among the three kinds of fishes,the proportion of somatotropin(STH)cells in triploids crucian carp was the highest,whereas that in allotetraploid hybrids was the *** results might be connected with the faster growth rate of triploids and slower growth rate of al-lotetraploid *** addition,in GTH cells of meso-adenohypophysis after the breeding season,there were many endocrine particles in triploids,while those endocrine particles were released from the cells in allotetraploids and *** result showed that the sterility of triploid crucian carp might be related to the hormone which was not released from the GTH *** a word,the present study indicated that the differences in the structure of pituitary among different ploidy level fishes contributed to their difference in the growth rate and gonadal development.

关键词： diploid triploid ailotetraploid pituitary histological structure ultra-structure

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A REVIEW ON RESEARCH ON DEVELOPMENT OF LIGAMENT CONSTITUTIVE RELATIONS ON MACRO, MESO, AND MICRO LEVELS

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Acta Mechanica Solida Sinica 2013年第4期26卷 331-343页

作者： Chao Wan Zhixiu Hao Shizhu Wen State Key Laboratory of Tribology Tsinghua University

Ligaments are densely connective soft tissues capable of maintaining stability and function of knee *** an important factor,the constitutive relation of ligament would affect its biomechanics and further play an essential role in the research on ligament injury,healing and *** objective of this paper is to provide an overview of the current research on ligament constitutive relations on the macro,meso,and micro levels as well as the anatomy and histological structure of *** studies of biomechanical behaviors during ligament injury and healing periods have also been *** on the research on ligament constitutive relation in the past three decades,a discussion of some research perspectives is also presented,such as a validated accurate measuring method of in situ strain in ligament,a new constitutive relation involving the distribution of ultra-structural properties,and a rational estimation of ligament injury and healing process by the change of its ultra-structural or histological characteristics.

关键词： constitutive relation ligament ultra-structure macro meso micro

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The Dynamics of Changes in Starch and Lipid Droplets and Sub-Cellular Localization of β-Amylase During the Growth of Lily Bulbs

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Journal of Integrative Agriculture 2012年第4期11卷 585-592页

作者： WU Sha-sha WU Jin-di JIAO Xue-hui ZHANG Qi-xiang LV Ying-min College of Landscape Architecture Beijing Forestry University Beijing 100083 P.R.China China National Research Center for Floriculture Engineering ＆ Technology Beijing 100083 P.R.China

The ultra-structure of mother and outer daughter scales of Lilium Oriental hybrid Sorbonne were studied using transmission electron microscope to examine the sub-cellular localization of starch and lipid droplets during growth and development from shoot emergence to *** contents of starch granules and lipid droplets in the cell of the mother scales decreased significantly from shoot emergence to anthesis,indicating that these scales served as a source for growth and *** flowering,the number of starch granules and lipid droplets increased dramatically,and finally the cells were filled with the above molecules indicating that the bulb becomes a major sink during bulb *** observation also showed that symplastic pathway is the main pathway in cells in the exchange and transportation of material during bulb *** activity of β-amylase,one of the key enzymes catalyzing starch breakdown,showed a similar *** enzyme sub-cellular localization via immune-gold electron-microscopy showed that βamylase was predominantly located together with starch granules,while the gold particles were scarcely found in other sub-cellular *** result suggested that this enzyme is compartmented together with its functional substrate supporting its function in catalyzing starch breakdown in living plant cells.

关键词： Lilium Oriental hybrids bulb scale source and sink ultra-structure β-amylase sub-cellular localization

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Structural and Functional Changes of Immune System in Aging Mouse Induced by D-Galactose

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Biomedical and Environmental Sciences 2006年第6期19卷 432-438页

作者： HONG-BIN DENG CHUN-LEI CHENG DA-PENG CUI DIAN-DONG LI LI CUI NIAN-SHENG CAI Institute of Medicinal Biotechnology Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100050 China Preclinical Medicine College Jilin University Changchun 130021 Jilin China

Objective To investigate the role of D-galactose, especially in the structural and functional changes of the immune system in aging. Methods Serum levels of advanced glycation end-products （AGE） were determined by ELISA method. ultra-structures of thymus and spleen were detected by transmission electron microscopy. MTT method was used to determine the lymphocyte proliferation. IL-2 activity was determined by bioassay. Northern blot was used to detect the IL-2 mRNA levels. Results Serum AGE levels of D-galactose- （P〈0.01） and AGE-treated （P〈0.05） mice （n=8） were increased significantly. The ultra-structures of thymus and spleen in D-galactose- and AGE-treated mice showed regressive changes similar to those in the aged control group. The lymphocyte mitogenesis and IL-2 activity of spleen were also decreased significantly （P〈0.01, n=8）. The change of IL-2 activity shown by Northern blot resulted from the change of mRNA expression. The AGE plus aminoguanidine group, however, showed no significant change in these parameters in comparison with the young control group （P〈0.01 or P〈0.05, n=8）. Conclusion D-galactose and AGE lead to a mimic regression change of aging in the immune system in vivo.

关键词： D-galactose Aging-mimetic model Advanced glycation end-products ultra-structure Nonenzyme glycation Aminoguanidine

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Antennal epicuticular structure of camel crickets (Orthoptera: Rhaphidophoridae) for identifying the prey of Mustella sibrica Pallas

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Advances in Entomology 2014年第1期2卷 1-7页

作者： Chun-Yu Hu Jeng-Tze Yang Wu-Chun Tu Department of Entomology National Chung-Hsing University Taichung Taiwan

Camel crickets (Orthoptera: Rhaphidophoridae) are a group of wingless and humpbacked Orthopteran insects. They are closed relatives to katydids and crickets;they were called spider crickets somewhere because of their long legs. Camel cricket plays an important role in the food web in various ecosystems. In the forest ecosystem, they live in the ground level of forestry under litters and fallen leaves, and some of them live beneath the log and hid themselves under loosen tree barks. They feed on decomposing organic substances and serve as decomposers in the food chain. It is a very common insect prey to insectivorous animals in natural and artificial vegetation. Guandaushi (GDS) is the experimental forest station of National Chung Hsing University, as the study site of long term ecological research (LTER) in Taiwan. And the camel cricket became an important subject and a dominant insect group in the LTER studies. They are the major parts of the insect preys of vertebrates, especially to the insectivorous mammals. According to our previous publication, 32.2% of the insect preys of Mustela sibirica Pallas are camel crickets in GDS forest ecosystem. There were 5 morpho-species of camel crickets by the characters of antennal epicuticular characters. The camel cricket was collected from various ways according to the LTER study during 1995-1998. The morphology of antennal epicuticular structure from totally 60 individuals was observed by using SEM. The epicuticular sculpture was described and classified into Type I and Type II by the SEM photographs. Type I has 8 patterns and Type II has 5 patterns in this study. The illustration and the pictorial key were provided here as the tool for further application. The morpho-species could not be identified due to the remainder being fragmented while examined. After the study, the SEM antennal epicuticular structures were classified and identified based upon the antennae sample of the morphospecies from the specimens of the 5 camel

关键词： Camel Crickets Antennal Epicuticle ultra-structure Insectivorous Prey

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Effect of Cistanche Desertica Polysaccharides on Learning and Memory Functions and ultrastructure of Cerebral Neurons in Experimental Aging Mice

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Chinese Journal of Integrative Medicine 2001年第4期7卷 288-292页

作者：孙云邓杨梅王德俊沈春锋刘晓梅张洪泉 Medical and Pharmacological Institute of Yangzhou University Jiangsu 225001

To observe the effects of Cistanche desertica polysaccharides (CDP) on the learning and memory functions and cerebral ultrastructure in experimental aging mice. Methods: CDP was administrated intragastrically 50 or 100 mg/kg per day for 64 successive days to experimental aging model mice induced by D-galactose, then the learning and memory functions of mice were estimated by step-down test and Y-maze test; organelles of brain tissue and cerebral ultrastructure were observed by transmission electron microscope and physical strength was determined by swimming test. Results: CDP could obviously enhance the learning and memory functions (P＜0.01) and prolong the swimming time (P＜0.05), decrease the number of lipofuscin and slow down the degeneration of mitochondria in neurons(P＜0.05), and improve the degeneration of cerebral ultra-structure in aging mice. Conclusion: CDP could improve the impaired physiological function and alleviate cerebral morphological change in experimental aging mice.

关键词： aging learning and memory functions Cistanche desertica polysaccharides ultra-structure D-galactose

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Cd^(2+)污染对水花生生理生化及超微结构的影响

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应用生态学报 2003年第9期14卷 1581-1584页

作者：周红卫施国新杜开和徐勤松徐楠南京师范大学生命科学学院南京210097

1 引言自1935年日本发生因镉中毒而导致的骨痛病以来,镉污染越来越受到人们的重视.有关其对人类及其它生物的危害已进行了很多研究[3,8,12].对水生植物而言,多数工作集中在对生理生化毒害[2,7]或对细胞结构损伤[7]的个别研究.笔者认为... 详细信息

1 引言自1935年日本发生因镉中毒而导致的骨痛病以来,镉污染越来越受到人们的重视.有关其对人类及其它生物的危害已进行了很多研究[3,8,12].对水生植物而言,多数工作集中在对生理生化毒害[2,7]或对细胞结构损伤[7]的个别研究.笔者认为把生理生化变化与结构的破坏联系起来.讨论两者变化的相互关系,探讨其本质,才符合此过程的实际,则更具现实意义.但这方面的研究在国内还不多见.

关键词： Cd^2+ Pollution of heavy metal Altemanthera philoxemides Antioxidant enzyme systems ultra-structure

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中国遗传学会“第十一届全国激光生物学学术会议”暨《激光生物学报》创刊廿周年庆祝会、第十届粤港生物物理研讨会暨2012年广东生物物理学术年会

作者： LIU Li~1,JIN Hua~1,PI Jiang~1,JIANG Jin-huan~1,KE Chang-hong~1,CAI Huai-hong~1,CAI Ji-ye~(1*) (1.Key Laboratory of Optoelectronic Information and Sensor Technologies of Guangdong Higher Education Institutes,Jinan University,Department of Chemistry,Guangzhou 510632,China)

Objective To investigate the apoptosis effect induced by the combination of cinobutacini and *** MTT assay was used to determine the growth inhibition. The rate of cell apoptosis was measured using flow *** was used to detect the changes in morphology and ultrastrucure of Hela *** The results indicated that the growth inhibition and apoptotic rate of Hela cells were 64.04±1.29%～75.07±2.19%and 11.97±1.36%～49.2±2.36%,respectively,induced by the combined effect of two drugs,which were significantly higher than the effect of cinobutacini alone(11.30%±0.6%～22.04%±0.96%and 8.05%±0.21%～16.43%±0.29%).And,the killing efficacy was in a dose-dependent manner. Simultaneously,AFM morphological imaging showed that the cell membrane of control cells represented compact structure,while it became broken or ruptured after treated with the drugs. Besides,the average roughness and grain diameter of the cell membrane also increased obviously with the drug *** Results manifested that the combined effect of cinobutacini and curcumin presented synergistic effect,which provided new ideas for the drug selection of treatments of cervical carcinoma.

关键词： cinobutacini and curcumin growth inhibition apoptosis atomic force microscopy(AFM) cell morphology ultra-structure average roughness

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Effects of Ginkgo biloba extract 50 on myocardial function in myocardium ischemic rats

Effects of Ginkgo biloba extract 50 on myocardial function i...

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中国生理学会第23届全国会员代表大会暨生理学学术大会

作者： Ai-Hua LIU,Yi-Min BAO,Xing-Yu WANG,Zhi-Xiong ZHANG~* Shanghai University of Traditional Chinese Medicine,Shanghai 201203

＜正＞Objective:To study the effects of Ginkgo biloba extract 50（GBE50） on myocardial function,area at risk and myocardial ultra-structure in myocardium ischemic ***:Myocardium ischemia was induced by the left anterior descending（LAD） coronary artery occlusion and myocardial function was *** vitro myocardial infarction was measured by 2,3,5-triphenyltetrazolium chloride（TTC） and Evans blue staining of heart *** hematoxylin and eosin（HE） staining was used for morphological evaluation by light microscopy. Results:Hemodynamic experiments in vivo showed that postischemic cardiac contractile function was reduced in ischemic ***（7.5 g/kg/d×7） and GBE50（100 mg/kg/d×7） improved postischemic cardiac diastolic dysfunction. The area at risk was significantly reduced and myocardial structure was better-preserved in hearts of GBE50 group and SM ***:GBE50 could attenuate myocardium damage induced by ischemia and provide potent myocardial protection.

关键词： GBE50 cardiac ischemia myocardial function area at risk ultra-structure

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