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Hydrophobin Protein from Trichoderma harzianum Induced Maize Resistance to Maize Leaf Spot Pathogen Curvularia lunata

Hydrophobin Protein from Trichoderma harzianum Induced Maize...

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中国植物病理学会2016年学术年会

作者： YU Chuan-jin LU Zhi-xiang XIA Hai GAO Jin-xin DOU Kai WANG Meng LI Ya-qian CHEN Jie Key Laboratory of Urban Agriculture(South) Ministry of AgricultureSchool of Agriculture and BiologyShanghai Jiao Tong University State Key Laboratory of Microbial Metabolism Shanghai Jiao Tong University

Corn Curvularia leaf spot disease was a frequently occurring disease to maize leaf,which caused by Curvularia lunata leaf spot pathogen,this disease was very common in corn planting area in ***,the prevention and treatment of the disease was of great ***,low molecular mass(about 10 kDa) secreted proteins of fungi,were characterized by moderate to high levels of hydrophobic material(hydrophobic substances) to cover on the surface of the microbial cells and the presence of eight conserved cysteine(Cys) residues and formed 4 disulfide bond.A class Ⅱ hydrophobin gene hyd1 was cloned.A 2kb fragment of the hyd1 promoter sequence upstream hyd1,hyd1 and DsRed were ligated *** expression cassete P:DsRed and P:hyd1:DsRed were introduced into the KO 16(hyd1 disruption transformant) and showed that hyd1 was localized in the plasma membrane by the lazer confocal fluorescense microscope.B73 seeds that treated with the conidia of different hyd1 transformants and grew in greenhouse,the 4-leaf stage leaves were inoculated *** in vitro and in *** results demonstrated that the maize leaf lesion size that treated by KO 16 was larger than WT(wild type),the disease index of the overexpression treatment(OE3 and OE5) maize was *** callose accumulation was increased in the maize leaves infiltrated with in vitro expressed Hyd1 protein comparied with *** also indicated that hyd1 influenced Trichoderma colonization in maize roots by the asssy of SEM and ***,Hyd1 protein was detected on the plasma membrane of maize root by the IEM and it was demonstrated that hyd1 acted as MAMPs(Microbial-associated molecular patterns) to induce the maize *** yeast bait plasmid pGBKT7-hyd1 was constructed and screened the maize root cDNA Library and found that hyd1 interacted with *** verified the two interaction proteins again by BiFC and pulll down method and also identified ubiquilinl and hyd1 interaction motif

关键词： Trichoderma harzianum Hydrophobin ubiquilinl MAMPs Protein interaction Induce systemic resistance

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