检索结果-南通市图书馆

IDENTIFICATION OF HuMAN MuRR1, THE HOMOLOGuE OF MOuSE IMPRINTED Murr1 GENE

Journal of Pharmaceutical Analysis 2006年第2期18卷 161-166页

作者：张忠明王又冬八木ひとみ城圭一郎岩坂刚向井常博 Department of Obstetrics & Gynecology the First Affiliated Hospital of Medical School Xi'an Jiaotong University Xi'an 710061 ChinaDepartment of Biomolecular Sciences Saga Medical School 5-1-1 Nabeshima Saga 849-8501 Japan Department of Obstetrics & Gynecology the First Affiliated Hospital of Medical School Xi'an Jiaotong University Xi'an 710061 ChinaDepartment of Obstetrics & Gynecology Saga Medical School 5-1-1 Nabeshima Saga 849-8501 Japan

Objective To identify the mRNA sequence, genetic construction, imprinting status, and expression profile of human MuRR1 gene, the homologue of mouse imprinted Murr1 gene. Methods The MuRR1 mRNA sequence was identified by colony hybridization screening of human cDNA library and the 5'-RACE analyses; Absence of u2af1-rs1 gene within MuRR1 was confirmed by Southern Blotting; Expression profile of MuRR1 was examined by Northern Blotting; The imprinting status of MuRR1 were revealed by SNP investigation and RT-PCR followed by sequencings and RFLP analyses. Results The full-length mRNA sequence of MuRR1 spans 711 bp, transcribed from 3 exons, encodes predicted MuRR1 protein of 190 amino acids. The gene was expressed in all the 12 kinds of human adult tissues and 6 kinds of fetal tissues. It showed biallelic expression in all 32 investigated samples including 6 kinds of human fetal tissues and 8 adult brains. unlike mouse imprinted u2af1-rs1 gene existing in the intron of Murr1, the human u2af1-rs1 gene was not located in the MuRR1 locus. Conclusion Human MuRR1 gene is not imprinted and the non-imprinting is possible due to the absence of human homologue of mouse u2af1-rs1 within MuRR1 locus.

关键词： Genomic imprinting MuRR1 u2af1-rs1

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：