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Polycytosine RNA-binding protein 1 regulates osteoblast function via a ferroptosis pathway in type 2 diabetic osteoporosis

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World Journal of Diabetes 2024年第5期15卷 977-987页

作者： Hong-Dong Ma Lei Shi Hai-Tian Li Xin-Dong Wang Mao-Wei Yang Department of Orthopedics The Affiliated Hospital of Nantong UniversityNantong 226001Jiangsu ProvinceChina Department of Orthopedics The First Hospital of China Medical UniversityShenyang 110001Liaoning ProvinceChina

BACKGROUND Recently,type 2 diabetic osteoporosis(T2DOP)has become a research hotspot for the complications of diabetes,but the specific mechanism of its occurrence and development remains *** caused by iron overload is con-sidered an important cause of *** RNA-binding protein 1(PCBP1),an iron ion chaperone,is considered a protector of *** To investigate the existence of ferroptosis and specific role of PCBP1 in the development of type 2 *** A cell counting kit-8 assay was used to detect changes in osteoblast viability under high glucose(HG)and/or ferroptosis inhibitors at different concentrations and *** electron microscopy was used to examine the morpho-logical changes in the mitochondria of osteoblasts under HG,and western blotting was used to detect the expression levels of PCBP1,ferritin,and the ferroptosis-related protein glutathione peroxidase 4(GPX4).A lentivirus silenced and overex-pressed *** blotting was used to detect the expression levels of the osteoblast functional proteins osteoprotegerin(OPG)and osteocalcin(OCN),whereas flow cytometry was used to detect changes in reactive oxygen species(ROS)levels in each *** Under HG,the viability of osteoblasts was considerably decreased,the number of mitochondria undergoing atrophy was considerably increased,PCBP1 and ferritin expression levels were increased,and GPX4 expression was *** blotting results demonstrated that infection with lentivirus overexpressing PCBP1,increased the expression levels of ferritin,GPX4,OPG,and OCN,compared with the HG *** cytometry results showed a reduction in ROS,and an opposite result was obtained after silencing *** PCBP1 may protect osteoblasts and reduce the harm caused by ferroptosis by promoting ferritin expression under a HG ***,PCBP1 may be a potential therapeutic target for T2DOP.

关键词： Polycytosine RNA-binding protein 1 Ferroptosis Reactive oxygen species Ferritin Osteoblast type 2 diabetic osteoporosis

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