检索结果-南通市图书馆

Screening and degradation characteristics of a tylosin-degrading strain

Journal of Integrative Agriculture 2020年第4期19卷 1127-1136页

作者： FENG Chang-qing CHENG Deng-miao FENG Yao QI Wei-ning JIA Zhen-hu Louise WEAVER LIU Yuan-wang LI Zhao-jun College of Life Science Shanxi Normal UniversityLinfen 041004P.R.China Key Laboratory of Plant Nutrition and Fertilizer Ministry of Agriculture and Rural Affairs/China-New Zealand Joint Laboratory for Soil Molecular EcologyInstitute of Agricultural Resources and Regional PlanningChinese Academy of Agricultural SciencesBeijing 100081P.R.China Institute of Environmental and Science Research Ltd. Christchurch Science CentreChristchurch 8041New Zealand

Antibiotics residues have been accumulating in the environment day by day due to overuse of *** antibiotic residues,such as tylosin(TYL),can cause serious environmental problems,which makes it important to eliminate TYL from the *** is important to eliminate TYL from the *** this study,a strain was isolated and purified from fermentation by-product that came from a TYL production *** TYL degrading strain was identified by its morphology,physiological and biochemical reactions and sequencing the PCR-amplified fragments of its 16 S r DNAcoding *** temperature,shaking speed,initial TYL concentration,p H and inoculum sizes were investigated under simulated conditions by using single factor *** results showed that TYL2,a high efficient strain was isolated and was identified as Brevibacillus *** degradation rate of TYL by this strain could reach to 75%with an initial concentration of 25 mg L^-1 within 7 days under conditions of 7%***(v/v,2×108 CFU m L^-1)at p H 7.0 and at 35°*** is interesting that this strain has a very strong ability to degrade the TYL in natural sewage with the degradation rate of 65%within 7 *** result could be helpful for the degradation of TYL and provide guidance for the degradation of other antibiotics.

关键词： antibiotics tylosin screening degrading strain B.borstelensis degradation characteristic

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Enhancing tylosin production by combinatorial overexpression of efflux,SAM biosynthesis,and regulatory genes in hyperproducing Streptomyces xinghaiensis strain

引用

Synthetic and Systems Biotechnology 2023年第3期8卷 486-497页

作者： Penghui Dai Yuyao Qin Luyuan Li Haidi Li Lihuo Lv Danying Xu Yuqing Song Tingting Huang Shuangjun Lin Zixin Deng Meifeng Tao State Key Laboratory of Microbial Metabolism School of Life Sciences and BiotechnologyShanghai Jiao Tong UniversityShanghai200240China Zhejiang Apeloa Biotechnology Co. Ltd.Jinhua322109China Zhejiang Apeloa Jiayuan Pharmaceutical Co. Ltd.Jinhua322118China Haihe Laboratory of Synthetic Biology Tianjin300308China

tylosin is a 16-membered macrolide antibiotic widely used in veterinary medicine to control infections caused by Gram-positive pathogens and *** improve the fermentation titer of tylosin in the hyperproducing Streptomyces xinghaiensis strain TL01,we sequenced its whole genome and identified the biosynthetic gene cluster *** of the tylosin efflux gene tlrC,the cluster-situated S-adenosyl methionine(SAM)synthetase gene metK_(cs),the SAM biosynthetic genes adoK_(cs)-metFcs,or the pathway-specific activator gene tylR enhanced tylosin production by 18%,12%,11%,and 11%in the respective engineered strains TLPH08-2,TLPH09,TLPH10,and ***-overexpression of metK_(cs)and adoK_(cs)-metFcs as two transcripts increased tylosin production by 22%in the resultant strain TLPH11 compared to that in ***,combinational overexpression of tlrC,metK_(cs),adoK_(cs)-metFcs,and tylR as four transcripts increased tylosin production by 23%(10.93g/L)in the resultant strain TLPH17 compared to that in ***,a negligible additive effect was displayed upon combinational overexpression in TLPH17 as suggested by the limited increment of fermentation titer compared to that in *** analyses indicated that the expression of tlrC and three SAM biosynthetic genes in TLPH17 was considerably lower than that of TLPH08-2 and *** on this observation,the five genes were rearranged into one or two operons to coordinate their overexpression,yielding two engineered strains TLPH23 and TLPH24,and leading to further enhancement of tylosin production over *** particular,the production of TLPH23 reached 11.35 g/*** findings indicated that the combinatorial strategy is a promising approach for enhancing tylosin production in high-yielding industrial strains.

关键词： Streptomyces xinghaiensis tylosin Combinational metabolic engineering

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Pharmacokinetics and Tissue Residues of tylosin in Broiler Chickens

引用

Pharmacology & Pharmacy 2016年第1期7卷 36-42页

作者： Ahmed M. Soliman Mahmoud Sedeik Department of Pharmacology Faculty of Veterinary Medicine Cairo University Giza Egypt Department of Poultry and Fish Diseases Faculty of Veterinary Medicine Alexandria University Alexandria Egypt

The pharmacokinetics and tissue residue of tylosin in broiler chickens were studied after I.V. and oral administrations in a dose of 50 mg tylosin/***. tylosin was obeyed a two-compartment open model following I.V. administration at a dose of 50 mg/***. The disposition kinetics of tylosin following I.V. administration revealed that tylosin was highly distributed with Vd(area) of 6 L/kg and eliminated with half-life (t1/2β) equal to 7.29 hours. The disposition kinetics of tylosin following oral administration revealed that the maximum blood concentration (Cmax) was 3.40 μg/ml attained at (tmax) of 1.08 hour. tylosin was eliminated with half-life (t1/2β) equal to 5.78 hours. The mean systemic bioavailability of tylosin after oral administration was 90.29%. Following repeated oral administration of 50 mg tylosin base/*** once daily for 5 consecutive days, the blood (μg/ml) and tissue (μg/g) residues of tylosin showed that liver, kidney and lung contained the highest tylosin residues and completely disappeared from those tissues at 6 days after the last oral dose. Chickens should not be slaughtered for human consumption within the treatment and 6 days after the last oral administrations of tylosin.

关键词： Pharmacokinetics tylosin Broiler Chickens Bioavailability Tissue Residue

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

应用螺旋霉素(Spiramycin)及泰勒菌素(tylosin)对鸡败血支原体病的治疗和预防试验

引用

中国预防兽医学报 1982年第1期 21-26页

作者：王永环周庭义李跃庭冯菊艳王蕴敏中国农业科学院哈尔滨兽医研究所

鸡败血支原休病是一种鸡的慢性呼吸道传染病(CRD),目前已广泛流行于世界各地。我国较大型的一些鸡场也有本病发生,死亡率虽不高,但严重影响产卵率和孵化率,尤其在幼龄雏鸡饲养管理不当或于冬春季节混感其他疾病时,则死亡率可达30～50%... 详细信息

鸡败血支原休病是一种鸡的慢性呼吸道传染病(CRD),目前已广泛流行于世界各地。我国较大型的一些鸡场也有本病发生,死亡率虽不高,但严重影响产卵率和孵化率,尤其在幼龄雏鸡饲养管理不当或于冬春季节混感其他疾病时,则死亡率可达30～50%左右。本病除以接触方式传播外,也可经卵垂直传递给后裔雏鸡,使一些鸡场长时发生本病,从而给养鸡事业造成很大的经济

关键词：预防试验鸡败血支原体 Spiramycin tylosin 泰勒支原体病平板凝集反应螺旋霉素抗菌素

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

泰乐菌素tylosin产品

引用

上海医药情报研究 1994年第2期 13-14页

作者：俞玉英五洲药厂

一、概述泰乐菌素tylosin又名泰霉素、泰兰霉素、主要产品是泰乐霉素磷酸盐,取自Strepeomyces fradial菌培养液的一种大环内酯类抗生素。本品外观为白色板状结晶,微溶于水,其酒石酸盐,磷酸盐等则易溶于水。水溶液中如有铁、铜、钼、锡... 详细信息

一、概述泰乐菌素tylosin又名泰霉素、泰兰霉素、主要产品是泰乐霉素磷酸盐,取自Strepeomyces fradial菌培养液的一种大环内酯类抗生素。本品外观为白色板状结晶,微溶于水,其酒石酸盐,磷酸盐等则易溶于水。水溶液中如有铁、铜、钼、锡等离子时,可与本品络合而失效,泰乐霉素系弱盐基性化合物容易制取盐酸盐,硫酸盐,乳酸盐等,能抑制与动物争食营养的微生物,还能促进饮水量及采食量。泰乐霉素具有广泛的微生物抗菌力,特别对革兰氏阳性菌有抑菌作用,它的最低抑菌浓度(微克/毫升): 金黄色葡萄球菌 209P 0.39—0.

关键词：泰乐菌素 tylosin 抗生素

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

大环内酯类抗生素泰乐菌素(tylosin)在分批和恒化器培养中的生产

引用

国外医药（抗生素分册） 1981年第4期9卷 94-94页

作者：杨乃权

本文作者通过使用一种可溶性的合成培养基分别在分批和恒化器培养的条件下研究了弗氏链霉菌（Streptomyces fradiae）NRRL2702菌种产生泰乐菌素及有关化合物的情况。分批培养时,随着泰乐菌素、大菌素（Macrocin）和雷洛霉素（Relomycin... 详细信息

本文作者通过使用一种可溶性的合成培养基分别在分批和恒化器培养的条件下研究了弗氏链霉菌（Streptomyces fradiae）NRRL2702菌种产生泰乐菌素及有关化合物的情况。分批培养时,随着泰乐菌素、大菌素（Macrocin）和雷洛霉素（Relomycin）在生产期的积累,观察到了生长期—生产期的动态模型。当菌种生长在恒化器时,发现泰乐菌素及有关化合物的比生产率（Q泰乐菌素）是生长率的函数。当 D=0.017小时-1时,Q 泰乐菌素为最大值。

关键词：泰乐菌素最大值生长率兽用药 tylosin 恒化器

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：