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Liver chimeric mice with tupaia hepatocyte transplantation as an animal model for hepatitis B virus infection and antiviral therapy

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Biosafety and Health 2019年第2期1卷 76-83页

作者： Lunzhi Yuan Yao Chen Xuan Liu Yali Zhang Ming Zhou Kun Wu Quan Yuan Tong Cheng Ningshao Xia State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics School of Public HealthXiamen UniversityXiamen 361102China National Institute of Diagnostics and Vaccine Development in Infectious Diseases School of Life SciencesXiamen UniversityXiamen 361102China

The human liver chimeric mouse is a milestone animal model for hepatitis B virus(HBV)*** mice with primary human hepatocyte(PHH)transplantation are adequate to support chronic HBV infection for several weeks and to evaluate antiviral ***,the drawbacks of PHHs include lack of available donors,poor expansion in vitro and ethical issues that limit the application of human liver chimeric mice,necessitating the search for ***,we transplanted primary tupaia hepatocytes(PTHs)into the livers of immunodeficient mice and achieved high liver chimerism within six *** tupaia liver chimeric mice are adequate to support chronic infection of the four common HBV genotypes A,B,C and D for 36 weeks,as well as evaluate of antiviral drugs,including hepatitis B immune globulin(HBIG),monoclonal antibody and nucleoside analogues(NAs),for preventative therapy and treatment post *** conclusion,the tupaia liver chimeric mouse model provides a convenient,efficient and stable animal model for chronic HBV infection and long-term drug evaluation.

关键词： tupaia Hepatitis B Liver chimeric mice Infectious animal model Antiviral therapy

清醒活动树鼩tupaia(Tree Shrew)神经元单位放电的记录

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贵州医科大学学报 1983年第1期40卷 31-32页

作者：孙公铎李绪明罗宗英曾万玲匡培梓张武田蔡景霞田芸芬贵阳医学院生理教研室脑与行为研究组四川泸州医学院生理学教研室贵阳中医学院生理学教研室中国科学院心理研究所生理心理病和病理心理研究室中国科学院昆明动物研究所灵长类研究室

鉴于原猿类的树鼩在进化中的特殊地位,有关它的神经生物学研究十分活跃。树鼩的单位放电研究已有报告(J. E. Abano et al. 1978;A. L. Humphrey et al. 1977、1980),但是采用慢性微电极技术记录行为状态的树鼩的单位放电方法迄今未见报... 详细信息

鉴于原猿类的树鼩在进化中的特殊地位,有关它的神经生物学研究十分活跃。树鼩的单位放电研究已有报告(J. E. Abano et al. 1978;A. L. Humphrey et al. 1977、1980),但是采用慢性微电极技术记录行为状态的树鼩的单位放电方法迄今未见报道,本文报告一种记录清醒活动状态树鼩的单位放电技术考虑到树鼩形体甚小、体重仅100多克。要分离单位放电,必须拥有一种特殊规格的微型微推进器,以适应特殊实验之需。本研究设计一种采用差动结构原理实现微推进的装置,整个装置由微推进器与基座组成。微推进器的参数如下:重量8.8克;微调范围:2.0毫米;微调读数5微米;微调可控范围:小于2微米;外形尺寸:13×13×51毫米。微推进器相对于基座有直径为1.8毫米的径向偏心移动范围。基座重0.65克,实验在局麻下,在立体定向仪控制下埋植基座。术后第二天即可实验观察。

关键词：树鼩单位放电 tupaia Tree Shrew 神经元神经组织

Chromosomal level assembly and population sequencing of the Chinese tree shrew genome

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Zoological Research 2019年第6期40卷 506-521页

作者： Yu Fan Mao-Sen Ye Jin-Yan Zhang Ling Xu Dan-Dan Yu Tian-Le Gu Yu-Lin Yao Jia-Qi Chen Long-Bao Lv Ping Zheng Dong-Dong Wu Guo-Jie Zhang Yong-Gang Yao Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences & Yunnan Province Kunming Institute of ZoologyChinese Academy of SciencesKunming Yunnan 650223China Center for Excellence in Animal Evolution and Genetics Chinese Academy of SciencesKunming Yunnan 650223China Kunming College of Life Science University of Chinese Academy of SciencesKunming Yunnan 650204China Kunming Primate Research Center of the Chinese Academy of Sciences Kunming Institute of ZoologyChinese Academy of SciencesKunming Yunnan 650223China KIZ–CUHK Joint Laboratory of Bioresources and Molecular Research in Common Diseases Kunming Institute of ZoologyChinese Academy of SciencesKunming Yunnan 650223China State Key Laboratory of Genetic Resources and Evolution Kunming Institute of ZoologyChinese Academy of SciencesKunming Yunnan 650223China Yunnan Key Laboratory of Animal Reproduction Kunming Institute of ZoologyChinese Academy of SciencesKunming Yunnan 650223China

Chinese tree shrews (tupaia belangeri chinensis) have become an increasingly important experimental animal in biomedical research due to their close relationship to primates. An accurately sequenced and assembled genome is essential for understanding the genetic features and biology of this animal. In this study, we used long-read single-molecule sequencing and high-throughput chromosome conformation capture (Hi-C) technology to obtain a high-qualitychromosome-scale scaffolding of the Chinese tree shrew genome. The new reference genome (KIZ version 2: TS_2.0) resolved problems in presently available tree shrew genomes and enabled accurate identification of large and complex repeat regions, gene structures, and species-specific genomic structural variants. In addition, by sequencing the genomes of six Chinese tree shrew individuals, we produced a comprehensive map of 12.8 M single nucleotide polymorphisms and confirmed that the major histocompatibility complex (MHC) loci and immunoglobulin gene family exhibited high nucleotide diversity in the tree shrew genome. We updated the tree shrew genome database (TreeshrewDB v2.0: http://***) to include the genome annotation information and genetic variations. The new high-quality reference genome of the Chinese tree shrew and the updated TreeshrewDB will facilitate the use of this animal in many different fields of research.

关键词： tupaia belangeri Chromosomal level assembly genome Population sequencing Database

A bright future for the tree shrew in neuroscience research:Summary from the inaugural Tree Shrew Users Meeting

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Zoological Research 2021年第4期42卷 478-481页

作者： Elise Savier Madineh Sedigh-Sarvestani Ralf Wimmer David Fitzpatrick University of Virginia CharlottesvilleVirginia 22903-1738USA Max Planck Florida Institute for Neuroscience JupiterFlorida 33458-2906USA Massachusetts Institute of Technology CambridgeMassachusetts 02139-4307USA

Tree shrews(tupaia spp.)have been used in neuroscience research since the 1960s due to their evolutionary proximity to *** use of and interest in this animal model have recently increased,in part due to the adaptation of modern neuroscience tools in this *** tools include quantitative behavioral assays,calcium imaging,optogenetics and *** facilitate the exchange and development of these new technologies and associated research findings,we organized the inaugural“Tree Shrew Users Meeting”which was held online due to the COVID-19 ***,we review this meeting and discuss the history of tree shrews as an animal model in neuroscience research and summarize the current themes being investigated using this animal,as well as future directions.

关键词： Tree Shrew Users Meeting Animal models tupaia Neuroscience

Advances in Animal Models of Hepatitis B Virus Infection

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国际感染病学（电子版） 2015年第4期4卷 96-101页

作者： Hang Zhang College of Medical Hangzhou Normal University

Hepatitis B virus (HBV) infection seriously affects human health. Stable and reliable animal models of HBV infection bear significance in studying pathogenesis of this health condition and development of intervention measures. HBV exhibits high specificity for hosts, and chimpanzee is long used as sole animal model of HBV infection. However, use of chimpanzees is strictly constrained because of ethical reasons. Many methods were used to establish small-animal models of HBV infection. tupaia is the only nonprimate animalthat can be infected by HBV. Use of HBV-related duck hepatitis virus and marmot hepatitis virus infection model contributed to evaluation of mechanism of HBV replication and HBV treatment methods. In recent years, development of human–mouse chimeric model provided possibility of using common experimental animals to carry out HBV *** models feature their own advantages and disadvantages and can be complementary in some ways. This

关键词： Hepatitis B virus animal model tupaia chimeric mice