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Expression of positive and negative regulators of cell cycle during wound healing

Chinese Medical Journal 2002年第3期115卷 326-330页

作者：朱旭东邸雁飞胡承香王正国第三军医大学野战外科研究所重庆400042

OBJECTIVE: To detect the expression of cell cycle positive regulators cyclin D(1), cyclin E, CDK(2), CDK(4) and negative regulators p21(cip1), p27(kip1), p16(ink4a) and p15(ink4b) during wound healing in rats. METHODS: Open wounds of full-thickness skin, diameter 1.8 cm, on rat backs were used as the wound model. Wound tissues were harvested on postwounding days 3, 5, 7, 9, 11, 14, 21 and 30. Ki67 expression in granulation tissue was detected by immunohistochemical assay. The patterns of the expression of cyclin D(1), cyclin E, CDK(2), CDK(4) and p21(cip1), p27(kip1), p16(ink4a), p15(ink4b) were detected by Western blot. RESULTS: Cell proliferation in granulation tissue took place predominantly within the first week after injury, with the proliferation peak occurring at postwounding day 5. There were no dramatic variations in the expression of cyclin D(1), CDK(2) and CDK(4) during wound healing. Up-regulated cyclin E was maintained from day 3 to 11 after injury, and then was down-regulated. No expression of p16(ink4a) and p15(ink4b) was found. p21(cip1) was expressed only from day 7 to 14, with peak expression observed on day 9. Constitutive p27(kip1) was expressed throughout wound healing with low levels in the proliferating period of day 3 to 5 and with increased levels in the post-mitotic and remodeling stage. The expression of p21(cip1) and p27(kip1) showed an inverse gradient to that of Ki67. CONCLUSION: p21(cip1) and p27(kip1) play a supervising role in preventing the hyperproliferative tendency in tissue repair.

关键词： Wound Healing Animals Cell Cycle Cell Cycle proteins Cell Division Cyclin-Dependent Kinase Inhibitor p16 Cyclin-Dependent Kinase Inhibitor p27 Cyclin-Dependent Kinases Cyclins Male Rats Rats, Wistar Research Support, Non-U.S. Gov't Skin tumor suppressor proteins

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Differential control of growth,apoptotic activity and gene expression in human colon cancer cells by extracts derived from medicinal herbs,Rhazya stricta and Zingiber officinale and their combination

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World Journal of Gastroenterology 2014年第41期20卷 15275-15288页

作者： Ayman I Elkady Rania Abd El Hamid Hussein Osama A Abu-Zinadah Department of Biological Sciences Faculty of SciencesKing Abdulaziz University Zoology Department Faculty of ScienceAlexandria University Department of Clinical Nutrition Faculty of Applied Medical SciencesKing Abdulaziz University Gamal Abd El Nasser Hospital

AIM: To investigate the effects of extracts from Rhazya stricta (R. stricta) and Zingiber officinale (Z. officinale) on human colorectal cancer cells.

关键词： Apoptosis Colorectal cancer Oncogenes Phytochemicals tumor suppressor proteins

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