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Detection of disseminated pancreatic cells by amplification of cytokeratin-19 with quantitative RT-PCR in blood,bone marrow and peritoneal lavage of pancreatic carcinoma patients

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World Journal of Gastroenterology 2007年第2期13卷 257-263页

作者： Katrin Hoffmann Christiane Kerner Wolfgang Wilfert Marc Mueller Joachim Thiery Johann Hauss Helmut Witzigmann Department of Visceral Transplantation Thoracic and Vascular Surgery University of Leipzig Germany Institute of Laboratory Medicine Clinical Chemistry and Molecular Diagnostics University of Leipzig Germany

AIM： To evaluate the diagnostic potential of cytokeratin-19 （CK-19） mRNA for the detection of disseminated tumor cells in blood, bone marrow and peritoneal lavage in patients with ductal adenocarcinoma of the pancreas. METHODS： Sixty-eight patients with pancreatic cancer （/7 = 37）, chronic pancreatitis （n = 16）, and non-pancreatic benign surgical diseases （/7 = 15, control group） were included in the study. Venous blood was taken preoperatively, intraoperatively and at postoperative d 1 and 10. Preoperative bone marrow aspirates and peritoneal lavage taken before mobilization of the tumor were analyzed. All samples were evaluated for disseminated tumor cells by CK-19-specific nested-PCR and quantitative fluorogenic RT-PCR. RESULTS： CK-19 mRNA expression was increased in 24 （64%） blood samples and 11 （30%） of the peritoneal lavage samples in the patients with pancreatic cancer. In 15 （40%） of the patients with pancreatic cancer, disseminated tumor cells were detected in venous blood and bone marrow and/or peritoneal lavage. In the peritoneal lavage, the detection rates were correlated with the tumor size and the tumor differentiation. CK-19 levels were increased in pT3/T4 and moderately/poorly differentiated tumors （G2/G3）. Pancreatic cancer patients with at least one CK-19 mRNA-positive sample showed a trend towards shorter survival. Pancreatic cancer patients showed significantly increased detection rates of disseminated tumor cells in blood and peritoneal lavage compared to the controls and the patients with chronic pancreatitis. CONCLUSION： Disseminated tumor cells can be detected in patients with pancreatic ductal adenocarcinorna by CK-19 fluorogenic RT-PCR. In peritoneal lavage, detection rate is correlated with tumor stage and differentiation. In the clinical use, CK-19 is suitable for the distinction between malignant and benign pancreatic disease in combination with other tumor-specific markers.

关键词： tumor cell dissemination Pancreatic cancer Cytokeratin-19

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Liquid biopsy in metastatic breast cancer

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Cancer Drug Resistance 2019年第4期2卷 1062-1068页

作者： Malgorzata Banys-Paluchowski Peter Paluchowski Department of Gynecology and Obstetrics Asklepios-Klinik Hamburg BarmbekHamburg 22307Germany Department of Gynecology and Obstetrics Regio Klinikum PinnebergPinneberg 25421Germany

The spread of single tumor cells shed by the primary tumor has been observed in most solid carcinomas and is generally associated with poor clinical *** cells detected in the peripheral blood are commonly referred to as circulating tumor cells(CTCs)and are seen as possible precursors of metastatic *** CTCs,circulating tumor DNA and non-coding RNA are increasingly the focus of translation cancer *** metastatic breast cancer(MBC),elevated levels of CTCs have been confirmed as an independent prognostic *** detection of elevated counts after the start of systemic therapy predicts poor response,it is unclear which treatment strategy should be offered in the case of CTC ***,the main potentials of blood-based diagnostics in BC are therapy monitoring and liquid biopsy-based treatment ***,the first positive study on CTC-guided therapy choices in hormone receptor positive HER2 negative MBC was *** the present review,we discuss the current data and potential clinical application of liquid biopsy in the metastatic setting.

关键词： tumor cell dissemination circulating tumor cell liquid biopsy circulating tumor DNA breast cancer

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