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Tioxolone niosomes exert antileishmanial effects on Leishmania tropica by promoting promastigote apoptosis and immunomodulation

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Asian Pacific Journal of tropical Medicine 2019年第8期12卷 365-374页

作者： Maryam Hakimi Parizi Iraj Sharifi Saeedeh Farajzadeh Abbas Pardakhty Mohammad Hossein Daie Parizi Hamid Sharifi Ali Reza Keyhani Mahshid Mostafavi Mehdi Bamorovat Ahmad Khosravi Daryoush Ghaffari Leishmaniasis Research Center Kerman University of Medical SciencesKermanIran Department of Pediatric dermatology Kerman University of Medical SciencesKermanIran Pharmaceutics Research Center Neuropharmacology InstituteKerman University of Medical SciencesKermanIran Department of Pediatrics Kerman University of Medical SciencesKermanIran HIV/STI Surveillance Research Center and WHO Collaborating Center for HIV SurveillanceInstitute for Futures Studies in HealthKerman University of Medical SciencesKermanIran Research Center of tropical and Infectious Diseases Kerman University of Medical SciencesKermanIran

Objective: To explore the antileishmanial effect of tioxolone and its niosomal form against Leishmania tropica. Methods: Tioxolone niosomes were prepared by the hydration method and were evaluated for morphology, size, release study, and encapsulation efficiency. The cytotoxicity of tioxolone and its niosomal form was measured by MTT assay, leishmanicidal activity against promastigote and amastigote by MTT assay, apoptosis by flow cytometry, IL-12, IL-10 and metacaspase gene expression levels by q-PCR. Results: Span/Tween 40 and Span/Tween 60 niosomes had good physical stability as depicted in their size distribution curves and high encapsulation efficiency(>99%). The release profile of the entrapped compounds showed Fickian’s model of tioxolone delivery based on diffusion through lipid bilayers. With the IC50 value for amastigote as(24.5±2.1) μg/mL and selectivity index as 10.5, the Span/Tween 60 niosome(NT2) had a superior effect to other drugs. The CC50 value and IC50 of promastigote value for NT2 were(257.5±24.5) μg/mL and(164.8±20.6) μg/mL, respectively. The flow cytometric analysis showed that tioxolone and niosomal forms induced apoptosis of Leishmania tropica promastigotes in a dose-dependent manner. NT2 increased the expression level of IL-12 and metacaspase genes and decreased the expression level of the IL-10 ***: Niosomes of tioxolone play an immunomodulatory role in increasing Th1 cytokine profile and inhibiting the Th2 cytokine profile. It could be used for treatment of anthroponotic cutaneous leishmaniasis.

关键词： Niosome Tioxolone Leishmania tropica Apoptosis

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Leishmania tropica: The comparison of two frequently-used methods of parasite load assay in vaccinated mice

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Asian Pacific Journal of tropical Biomedicine 2020年第6期10卷 248-253页

作者： Fatemeh Nemati Zargaran Mosayeb Rostamian Alisha Akya Hamid MNiknam Infectious Diseases Research Center Health InstituteKermanshah University of Medical SciencesKermanshahIran Immunology Department Pasteur Institute of Iran69 Pasteur AvenueTehran 13169-43551Iran

Objective:To compare limiting dilution assay and real-time PCR methods in Leishmania tropica parasite load measurement in vaccinated ***:BALB/c mice were vaccinated by Leishmania tropica soluble Leishmania antigen or recombinant Leishmania tropica stressinducible protein-1 with/without *** three vaccinations,mice were challenged by Leishmania tropica *** months after challenge,the draining lymph nodes of mice footpad were removed and parasite load was assayed by limiting dilution assay and real-time PCR *** dilution assay was done by diluting tissue samples to extinction in a biphasic *** real-time PCR,DNA of the lymph nodes was extracted,equal dilutions of each sample were prepared and hot-start real-time PCR was done using appropriate *** data of the two methods were compared by appropriate statistical ***:Both methods were able to measure different levels of parasite load in vaccinated/unvaccinated *** addition,wherever parasite load of a group was estimated high(or low)by one method,the estimated parasite load by another method was the same,although statistically significant differences were found between some ***:Both methods lead to approximately similar results in terms of differentiating parasite load of the experimental ***,due to the lower errors and faster process,the real-time PCR method is preferred.

关键词： Leishmania tropica Vaccinated mice Limiting dilution assay Parasite load assay,Real-time PCR

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First report on natural Leishmania infection of Phlebotomus sergenti due Leishmania tropica by high resolution melting curve method in Southeastern Iran

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Asian Pacific Journal of tropical Medicine 2014年第2期7卷 93-96页

作者： Aghaei Afshar A Rassi Y Sharifi I Vatandoost H Mollaie HR Oshaghi M.A Abai MR Rafizadeh S Leishmaniasis Research Center Kerman University of Medical Sciences Department of Medical Entomology & Vector Control School of Public HealthTehran University of Medical Sciences Department of Medical Virology Tehran University of Medical Sciences Department of Deputy of Strategic Planning Reference Health Laboratories Research CenterDeputy of Treatment.Ministry of Health & Medical Education of Health

Objective:To identify the Leishmania species in infected sand flies by Real-time PCR coupled with HRM ***:Real-time PCR coupled with HRM analysis targeting the first internal transcribed spacer(ITS1)of nuclear ribosomal DNA as the genetic marker was used to identify and distinguish Lelthmania species in sand flies ***:Three out of 115females of Phlebotomus sergenti(***)(2.6%)were positive to Leishmania tropica(***).Conclusions:This is the first report on *** as the main and proven vector of anthroponitic cutaneous leishmaniasis in Dehbakri County using Real-time PCR coupled with HUM *** method is rapid,sensitive and specific for diagnosing of parasites in infected Sand flies and ideal for large scale genotyping projects.

关键词： Vector(s) Leishmania tropica Real-time PCR Iran

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Effects of different colours in the visible region on <i>Leishmania tropica</i>

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Advances in Bioscience and Biotechnology 2011年第5期2卷 380-384页

作者： Samina. T. Yousuf Azeemi S. Mohsin Raza Masoom Yasinzai A. Samad Manzoor Iqbal Khattak 不详

The aim of this study was to investigate the wavelength-dependency of chromotherapy effects on cutaneous Leishmaniasis parasite growth. Chromotherapy uses visible range radiations to improve healing;however, its effects on parasite are not well understood. Leishmania tropica was irradiated using seven (7) different wavelengths of visible region. Optical density was observed, which showed that red colour (644 nm) wavelength inhibited the growth of parasite while other colour wavelengths also affected the growth of parasite. It is, therefore, suggested that as red colour inhibits the growth of parasite so patients suffering from *** can be treated with the application of red colour.

关键词： Leishmania tropica Chromotherapy Photo Stimulation

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First molecular identification of Leishmania species in a new endemic area of cutaneous leishmaniasis in Lorestan,Iran

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Asian Pacific Journal of tropical Medicine 2013年第9期6卷 713-717页

作者： Farnaz Kheirandish Ali Chegeni Sharafi Bahram Kazemi Mojgan Bandehpour Mohamad javad Tarahi Ali Khamesipour Razi Herbal Medicines Research Center Department of Parasitology and MycologyLorestan University of Medical Sciences Department of Communicable Disease Control and Prevention Deputy of HealthLorestan University of Medical Sciences Cellular and Molecular Biology Research Center Shahid Beheshti University Department of Parasitology and Mycology School of MedicineShahid Beheshti University of Medical Sciences Department of Biotechnology School of MedicineShahid Beheshti University of Medical Sciences Department of Epidemiology School of Public HealthTehran University of Medical.Sciences Center for Research and Training in Skin Disease and Leprosy Tehran University of Medical Sciences

Objective:To identify Leishman{u using ***:This studs was conducted from April2009 to March 2011 in order to identify Leishmania species in a new endemic area of CL in *** were taken from 62 patients that referred to the health centers in different cities of Lorestan province,the presence of Leishmcania was confirmed using direct smear and then grown in NNN media and mass cultured in RPM!1640 medium supplemented with 10%heat-inactivated fetal bovine *** was extracted from cultured promastigotes and used in ***:45(72.6%)samples out of 62 showed a hand in the range of 485 hp and 17(27.4%)with a hand in the range of 626 hp which were similar to standard strains of Leichmania tropica(*** and Leishnrania major(***),respectively.50(65.80%)of samples were collected from people with no history of travel in at least a year prior to the onset which shows that indigenous source of ***:Since the vector and reservoir of the two species are *** precise and extensive control and prevention methods should be designed and earned out.

关键词： Cutaneous leishmaniasis Leishmania tropica Leishmania major PCR Lorestan Iran

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