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Whole-tissue 3D imaging reveals intra-adipose sympathetic plasticity regulated by NGF-trka signal in cold-induced beiging

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Protein & Cell 2018年第6期9卷 527-539页

作者： Ying Cao Huanhuan Wang Wenwen Zeng Center for Life Sciences Tsinghua University Beijing 100084 China Institute for Immunology and School of Medicine Tsinghua University Beijing 100084 China Beijing Key Laboratory for Immunological Research on Chronic Diseases Beijing 100084 China Academy for Advanced Interdisciplinary Studies Peking University Beijing 10()8:71 China School of Life Sciences Peking University Beijing 100871 China

Sympathetic arborizations act as the essential efferent signals in regulating the metabolism of peripheral organs including white adipose tissues （WAT）. However, whether these local neural structures would be of plastic nature, and how such plasticity might participate in specific metabolic events of WAT, remains largely uncharacterized. In this study, we exploit the new vol- ume fluorescence-imaging technique to observe the significant, and also reversible, plasticity of intra-adi- pose sympathetic arborizations in mouse inguinal WAT in response to cold challenge. We demonstrate that this sympathetic plasticity depends on the cold-elicited sig- nal of nerve growth factor （NGF） and trka receptor. Blockage of NGF or trka signaling suppresses intra- adipose sympathetic plasticity, and moreover, the cold- induced beiging process of WAT. Furthermore, we show that NGF expression in WAT depends on the cate- cholamine signal in cold challenge. We therefore reveal the key physiological relevance, together with the reg- ulatory mechanism, of intra-adipose sympathetic plas- ticity in the WAT metabolism.

关键词： sympathetic plasticity NGF trka receptor cold-induced beiging whole-tissue 3D imaging

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Identification of astroglial heterogeneity in response to NGF stimulation and transplantation of NGF-induced O-2A lineage astroglia contribute to attenuating depressive-like behaviors in a rat frontal lobe injury model

Identification of astroglial heterogeneity in response to NG...

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中国神经科学学会第十二届全国学术会议

作者： Ye ZHANG Ling-Ling SHEN Huan-Huan ZHANG Min XU Chun-Lin XIA Department of Anatomy & Cytoneurobiology Unit Medical College of Soochow University

Objective Previous studies have shown that cerebral cortex-derived astrocytes can be isolated and induced in vitro into two morphologically and antigenically distinct astroglial populations: type-1 astrocytes(T1 As) and type-2 astrocytes(T2 As).Understanding how these resident glial cells respond to the environment is crucial to understanding how their functions in the CNS and how to best promote their therapeutic proliferation and differentiation. Methods The interaction between NGF and two astroglial lineages was identified by western blot assays and immunoflurence assays. By grafting NGF-induced O-2 A lineage astroglia into the rat frontal lobe injury model, the effects were measured by behavioral tests and western blot assays. Results(1) We firstly identified NGF-Trk A signaling via up-regulation of phosphorylation levels of Erk1/2 and Akt can greatly promote proliferative capacity of T2 As and became more induced into Ch AT/TH/Vimentin neurons with high efficiency, concomitant with the progressive activation of FEZ1, one highly-expression gene in O-2 A lineage astroglia previously verified by us. In contrast, we found that NGF treatment led to inhibit cell growth and changed morphology of T1 As, accompanied by up-regulation of p75 and Bax whereas down-regulation of GFAP, ALDH1 L1, GLT1, FEZ1 and NF-k B.(2) By grafting these NGF-induced T2 As into lateral ventricle in a rat frontal lobe injury model, these rodents displayed remarkable attenuation of depressive-like behaviors. Simultaneously in the injured cortex and hippocampus tissue of the grafting group, Trk A and its downstream signals expression were obviously up-regulated, as well as the expression of GAD67, a GABAergic neuronal marker. Strikingly, the expression of psychiatric susceptibility gene DISC1,FEZ1 and its key partner molecules including Kinesin5 B, Stx-1, Munc18 were significantly up-regulated in the cell-tranplant group. Conclusion Different responses into NGF in the two diverse astrocyte linea

关键词： astrocyte NGF trka receptor FEZ1 frontal lobe injury depression

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