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Optimization of solid fermentation of cellulase from trichoderma koningii

Journal of Environmental Sciences 2004年第5期16卷 816-820页

作者： LIPei-jun JINGDe-bing ZHOUQi-xing ZHANGChun-gui InstituteofAppliedEcology ChineseAcademyofSciences.Shenyang110016China GraduateSchooloftheChineseAcademyofSciences Beijing100039China

To exploit peashrub resources in Ordos as fodders, it is very crucial to realize industrial production of cheap cellulase of high activity by optimizing culture technology, especially culture substrate. In this study, a new prescription experiment based on uniform design ideal was invented and successfully applied in the solid fermentation of trichoderma koningii F244, which was performed with two different temperature degrees. The activities of FPA, cotton lyase, CMCase and β-glucosidase were assayed and then mathematical models of enzymatic activities, which were figured out by Unconstraint Mathematical Programming, were developed by Multivariate Regression Program of SPSS10.0. Enzymatic activities of optimized substrate prescriptions corresponding to mathematical models were forecasted to determine an ideal substrate prescription. It is revealed that in solid fermentation, Tween80 has negative effect on cellulase production. Furthermore, the ideal prescription for cellulase complex production by trichoderma koningii F244 was straw powder 16.9%,wheat bran 26.5%, (NH 4) 2SO 4 9.5% and water 47.1%, whose corresponding cellulase activity was expected to be at the same high level with that of trichoderma reesei Q9414 on its own recommended substrate. Especially, goats mainly fed on peashrub tissues mixed with cellulase complex of this prescription and culture technology, got an incremental ratio of 0.3 kg/d, which brought a very promising feeding prospect for local peashrub resource. By populization of this cellulase complex, it can integrate living standard, economic construction of local residents into vegetational restoration tightly and thus this paper will be very meaningful to be use for reference for western China like Ordos to realize its sustainable development of economy, society and environment.

关键词： trichoderma koningii substrate prescription FPA CMCase cotton lyase β-glucosidase

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Biosynthesis of Silver Nanoparticles by Septoria apii and trichoderma koningii

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Chinese Journal of Chemistry 2013年第4期31卷 529-533页

作者： Weidong Huang Jingjing Yan Yue Wang Cailing Hou Tianchun Dai Zhimin Wang School of Agriculture and Biology Shanghai Jiao Tong University Shanghai 200240 China

Silver nanoparticles were synthesized by Septoria apii and trichoderma koningii for the first time and charac- terized systematically.

关键词： silver nanoparticles Septoria apii trichoderma koningii

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康氏木霉(trichoderma koningii)纤维素酶系中β-葡萄糖苷酶的提纯与性质

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Acta Biochimica et Biophysica Sinica 1980年第3期 293-300页

作者：汪大受张静娟那安姜兆元中国科学院微生物研究所中国科学院微生物研究所北京

从康氏木霉培养抽提液中分离提纯了β-葡萄糖苷酶。提纯步骤通过DEAE SephadexA-50、SE Sephadex C-50和Sephadex G-75柱层析三步纯化。提纯后比活提高766倍,回收率为25.8%。经硷性和酸性Disc电泳以及SDS凝胶电泳鉴定均为单一带。用SDS... 详细信息

从康氏木霉培养抽提液中分离提纯了β-葡萄糖苷酶。提纯步骤通过DEAE SephadexA-50、SE Sephadex C-50和Sephadex G-75柱层析三步纯化。提纯后比活提高766倍,回收率为25.8%。经硷性和酸性Disc电泳以及SDS凝胶电泳鉴定均为单一带。用SDS凝胶电泳测得该酶的分子量为77000。用加温、改变pH或化学变性剂(SDS)处理均能使酶失活并且改变该酶的凝胶电泳行为,揭示该酶有亚基存在的可能性。用底物(纤维二糖)或竞争性抑制剂(葡萄糖酸δ-内酯)能保护该酶使其酶活和电泳行为基本上不改变或变化较小。δ-内酯的K比K要小二到三个数量级,它很可能是该酶底物过渡态中间物的类似物。

关键词： β-葡萄糖苷酶竞争性抑制剂康氏木霉 trichoderma koningii 纤维素酶系酶液纤维二糖酶活凝胶电泳

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Fermentation characteristics of vinegar residue and some natural materials

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Forestry Studies in China 2006年第3期8卷 22-25页

作者： Liu Jian Yang Ji-chu Institute of Biochemical Engineering Department of Chemical Engineering Tsinghua University Beijing 100084 P. R. China

Solid state fermentation is an attractive process to produce cellulase economically due to its lower financial investment and lower operating costs. Generally available natural materials in our study included vinegar residue, wheat bran, rice bran and wheat straw. Cellulase production was carried out by solid state fermentation using these materials as the substrate of trichoderma koningii. The ingredients of natural materials, associated with the effects of water content, time and nitrogen sources on cellulase synthesis were studied. A comparatively high cellulase activity （〉4 IU·g^-1 SDM） was obtained in the fermentation batch. The optimum culture time of vinegar residue, wheat bran and wheat straw were 72 hours, but that office bran was 60 hours. Total water contents of wheat bran, rice bran and wheat straw should not exceed 50% and that of vinegar residue should not be more than 60%. It was also shown that nitrogen salts contributed much to fermentation. （NH4）3PO4 and urea achieved good results in promoting enzyme activity.

关键词： natural materials vinegar residue cellulase solid state fermentation trichoderma koningii

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