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Chinese Medical Sciences Journal 1995年第3期10卷 131-135页

作者：王虹张月清劳为德吴赵永 BeijingTropicalMedicineResearchInstitute Beijing100050 BiophyslealhstituteofChineseAcademyofSciences Bei-jing100101

Five restriction endonucleases were used to digest genomic DNA from 5 isolates of trichinella spiralis obtained from Changchun, Tianjin, Xian, Henan and Yunnan. All the isolates were secured from pigs except the Changchun strain which came from dog. The DNA fragments digested by endonuclease were separated by agarose gel electrophoresis. The Changchun isolate had a EcoRI band at 1. 12kb and a DraI band at 1. 97kb which were unique to this isolate. A cloned specific repetitive DNA sequence (1. 12kb) from the Changchun strain was selected to prepare a probe for the Southern blotting of EcoRI restriction DNA fragments for the 5 isolates. The 1.12kb hybridizing band did not appear except in the Changchun *** results seem to indicate that there are differences between the isolates obtained from hosts in different geographical regions.

关键词： trichinella restriction fragment length polymorphisms Southern blot

Phenotypic Characterization of the Response to Infection with trichinella spiralisin Genetically Defined Mouse Lines of the CBi-IGE Stock

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Open Journal of Veterinary Medicine 2015年第5期5卷 111-122页

作者： María D. Vasconi Griselda Bertorini Ana V. Codina Paula Indelman Ricardo J. Di Masso Lucila I. Hinrichsen Instituto de Genética Experimental Facultad de Ciencias Médicas Universidad Nacional de Rosario Rosario Argentina rea Parasitología Facultad de Ciencias Bioquímicas y Farmacéuticas Universidad Nacional de Rosario Rosario Argentina CIC-UNR Universidad Nacional de Rosario Rosario Argentina

trichinella spiralis (T. spiralis), which is a cosmopolitan nematode that infects humans among other species, presents a complex host-parasite relationship that hinders the development of tools to eradicate the parasitosis. The aim of this research was to analyze the host response during a primary infection with T. spiralis in five genetically different mouse lines of the CBi-IGE stock. Adult males from the CBi+, CBi−, CBi, CBi/L and CBi/C lines were infected with 1, 2 or 4 L1 larvae per g of body weight. In the chronic stage, the number of parasites per g of tissue (relative larval load, rLL) showed a significant host genotype-dose interaction, since it did not increase in the same way in the five genotypes. At the lowest dose, both CBii−and CBi/L mice were resistant while CBi+, CBi/C, and CBi were susceptible. At the highest dose, only CBi/L remained resistant, and CBi+ was the most susceptible. The reproductive capacity index of adult worms (RCI = rLL/infective dose) evinced only a genotype effect, allowing rating each line as resistant or susceptible regardless of dose. Animals receiving 2 L1 larvae were also sacrificed in the intestinal phase (6 and 13 days p-i) to determine the number of adult parasites (nAP) recovered in a small intestine segment, and female fecundity (Ff). No differences in nAP were observed among genotypes on day 6 p-i. nAP decreased between days 6 and 13 p-i, this reduction being different among genotypes and significant only in CBi/L and CBi/C. Ff decreased in CBi/L and CBi/C on day 13 p-i. At the time of infection, serum cytokine baseline values showed a Th1 orientation for genotype CBi/L (high IFN-γ and IL-2) and a Th2 for CBi+ (high IL-4 and IL-10).The variability in the response observed in this murine model suggests its potential usefulness to gain insight into the mechanisms that regulate host-parasite relationship.

关键词： Host Genotype-Parasite Relationship Gastrointestinal Nematode trichinella spiralis Infectivity Resistance

Analysis of Diagnostic Efficiency of Excretory-Secretory Antigens for trichinella spiralisand trichinella nativain ELISA

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Open Journal of Veterinary Medicine 2014年第9期4卷 183-189页

作者： Irina Odoevskaya Sergey Movsesyan Mikhail Voronin Nataya Chiljuta Center of Parasitology A.N. Severtsov Institute of Ecology and Evolution Russian Academy of Sciences Moscow Russian Federation K.I. Skryabin All Russian Institute of Helminthology Agricultural Academy of Sciences Moscow Russian Federation

The comparative studies of diagnostic efficiency of excretory-secretory antigens of trichinella spiralis and trichinella nativa were performed using blood sera of rats from Wistar line experimentally infected with Arctic trichinellae. For animal infection and antigen preparation trichinella from muscles of wild carnivorous mammals from Arctic regions of Russia were used. When antigen from T. nativa larvae was used to analyze titers of sera of rats experimentally infected with Arctic trichinella, a significant increase in efficacy ofELISAwas detected. E.g., sera of rats infected with trichinellae from ringed seals retained in ELISA with T. nativa antigen values higher than diagnostic level at titers of 1:6400 - 1:12800, while titer of those same sera when using T. spiralis antigen was no higher than 1:200 - 1:400.

关键词： ELISA trichinella nativa Diagnostic Efficiency Laboratory Infection Wistar Rats Blood Sera Excretory-Secretory Antigen

Preliminary study of immunodiagnosis of trichinosis by ELISA using urea-soluble antigen of trichinella spiralis

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Journal of Medical Colleges of PLA(China) 1991年第1期6卷 84-87,92页

作者：张津利赖福春 Department of Parasitology Third Military Medical CollegeChongqing 630038

Urea-soluble antigens of trichinella spiralis infected larva were preparedand the efficiency of the antigens in diagnosis of trichinosis by ELISA *** IgG antibodies were detected by ELISA using urea-solubleantigens in 91.67% (22/24) of 24 serum samples taken from patients withtrichinosis and 91.67% (11/12) of 12 blood spots on filter *** IgMantibodies were seen in 95.24% (20/21) of 21 serum samples from patients withtrichinosis using the *** of patients infected with Schistosoma japonicumhad some cross teactions to the antigens,but the sera of mice infected withSchistosoma japoni(?) Ancylostoma caninum larva and Plasmodium yoelti were allnegative in the test.

关键词： trichinosis FLISA antigens trichinella spiralis

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利用改进的一步法提取本地毛形线虫总RNA

郑州牧业工程高等专科学校学报 2007年第2期27卷 1-2,5页

作者：郑宝亮付守申郜相君马辉郑鸣赵绪永郭宏伟杨向科郑州牧业工程高等专科学校生物工程系河南郑州450011 大庆油田物业集团黑龙江大庆163453

利用胃蛋白酶消化小鼠肌肉,获取了旋毛虫种本地毛形线虫(***)的肌幼虫虫体。利用TRIZOL混合虫体直接提取肌幼虫的总RNA,通过分光光度计Ultrospec 3000检测结果,以及用RT-PCR方法扩增出了编码***- tiva 49 kDa的ES蛋白结构基因,后续试验... 详细信息

利用胃蛋白酶消化小鼠肌肉,获取了旋毛虫种本地毛形线虫(***)的肌幼虫虫体。利用TRIZOL混合虫体直接提取肌幼虫的总RNA,通过分光光度计Ultrospec 3000检测结果,以及用RT-PCR方法扩增出了编码***- tiva 49 kDa的ES蛋白结构基因,后续试验结果显示改进的一步法提取本地毛形线虫总RNA效果很理想。

关键词： trichinella nativa 肌幼虫总RNA

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INFECTIOUS DISEASE

China Medical Abstracts(Internal Medicine) 1999年第2期16卷 69-72页

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云南省旋毛虫病流行病学及防治研究概况

中国寄生虫学与寄生虫病杂志 1991年第S1期9卷 85-88页

作者：杨洪模云南省卫生防疫站

1964年西藏首次报道我国人体感染旋毛虫病例,同年本省勐海县白文翰从2例患者活检中检出旋毛虫幼虫包囊而证实,由于本省多数民族(包括部分汉族)有喜吃生肉的陋习,致使本病流行严重程度居全国之首。现已查出12种动物为旋毛虫的自然宿主。... 详细信息

1964年西藏首次报道我国人体感染旋毛虫病例,同年本省勐海县白文翰从2例患者活检中检出旋毛虫幼虫包囊而证实,由于本省多数民族(包括部分汉族)有喜吃生肉的陋习,致使本病流行严重程度居全国之首。现已查出12种动物为旋毛虫的自然宿主。采用ELISA试验检测血清中抗体,Dot-ELISA试验检测血清中循环抗原。开展了猪肉内旋毛虫的加热及冷冻无害化处理等研究,现综述于下。

关键词： Parasite trichinella incidence control measure.

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旋毛虫感染的细胞免疫

中国微生态学杂志 1993年第4期5卷 58-62页

作者：蓝雁陈佩惠首都医学院北京100045

旋毛形线虫(trichinella spiralis)的免疫学研究开始于二十年代,七十年代以后研究逐渐深入。动物对旋毛虫的免疫反应表现为引起成虫发育不全,生殖力下降,成虫外排,新生蚴及侵入肌肉内的幼虫数目减少,对再感染有一定的抵抗力。本文就旋... 详细信息

旋毛形线虫(trichinella spiralis)的免疫学研究开始于二十年代,七十年代以后研究逐渐深入。动物对旋毛虫的免疫反应表现为引起成虫发育不全,生殖力下降,成虫外排,新生蚴及侵入肌肉内的幼虫数目减少,对再感染有一定的抵抗力。本文就旋毛虫的细胞免疫及其近年研究概况简述如下:

关键词：旋毛虫感染细胞免疫免疫学研究免疫反应旋毛形线虫新生蚴 spiralis 肌肉内发育不全 trichinella

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淮南猪肌肉旋毛虫病调查报告

河南畜牧兽医（综合版） 1992年第4期21卷 50-51页

作者：杨文明陈宏智周庆文

旋毛虫病(Trichinellosis)是由旋毛虫(trichinella spjralis)感染引起的一种严重的人畜共患寄生虫病,流行广泛,危害严重。猪为旋毛虫的最常见性宿主,其成虫寄生于肠壁粘膜上,称肠旋毛虫;幼虫寄生于横纹肌内,称肌旋毛虫。肌旋毛虫对猪危... 详细信息

旋毛虫病(Trichinellosis)是由旋毛虫(trichinella spjralis)感染引起的一种严重的人畜共患寄生虫病,流行广泛,危害严重。猪为旋毛虫的最常见性宿主,其成虫寄生于肠壁粘膜上,称肠旋毛虫;幼虫寄生于横纹肌内,称肌旋毛虫。肌旋毛虫对猪危害十分严重,猪感染后主要以骨胳肌内形成包囊结构为主要特征。

关键词：旋毛虫病 trichinella 猪肌肉人畜共患寄生虫自然感染率旋毛虫感染常见性消化法驱虫药肉品检验

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检疫和净化旋毛虫自然疫源地的探讨

云南畜牧兽医 1989年第2期 4-5,8页

作者：李全福施泽柱刘云山耿马县畜牧兽医站

旋毛虫病(Trichinosis)是由旋毛形线虫(trichinella spivalis)引起人畜共患的一种侵袭病。其寄生宿主领域广泛,感染包括人在内的120多种哺乳动物。严重威胁人类健康,並给畜牧业经济造成巨大损失。1980年1月耿马县首次在勐撒检出人和其... 详细信息

旋毛虫病(Trichinosis)是由旋毛形线虫(trichinella spivalis)引起人畜共患的一种侵袭病。其寄生宿主领域广泛,感染包括人在内的120多种哺乳动物。严重威胁人类健康,並给畜牧业经济造成巨大损失。1980年1月耿马县首次在勐撒检出人和其他哺乳动物旋毛虫以来,先后又在该县的一些区、乡陆续发生人因吃“酸肉”“红生”而感染旋毛虫的病例,其中尤以勐简乡的大寨严重。从1984年至今,先后发生四起旋毛虫病。为保护人类健康,我们对旋毛虫自然疫源地大寨村进行了调查、检疫和净化工作,现报告如下:

关键词：自然疫源地旋毛虫病 trichinella 旋毛形线虫畜牧业经济勐简净化工作耿马县压片镜检净化措施