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Purification,chemical modification and immunostimulating activity of polysaccharides from tremella aurantialba fruit bodies

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2010年第6期11卷 437-442页

作者： Xiu-ju DU Jing-song ZHANG Yan YANG Qing-jiu TANG Wei JIA Ying-jie PAN Institute of Edible Fungi Shanghai Academy of Agricultural Sciences Shanghai 201106 China College of Life Science Liaocheng University Liaocheng 252059 China College of Food Science Shanghai Ocean University Shanghai 200090 China

Ultrafiltration and a series of chromatographic steps were used to isolate and purify polysaccharides from tremella aurantialba fruit *** crude fractions(TAP50w,TAP10-50w,and TAP1-10w),five semi-purified fractions(TAPA-TAPE),and one purified fraction(TAPA1) were obtained.A sulfated derivative of TAPA1(TAPA1-s) was prepared by chemical *** immunostimulating activity of the polysaccharide fractions in vitro was determined using the mouse spleen lymphocyte proliferation *** the three crude fractions tested,cell prolifera-tion rates were increased most by ***,TAPA1-s was markedly more stimulatory than TAPA1,indicating that sulfonation was an effective way to enhance the immunostimulating activity of polysaccharide.

关键词： tremella aurantialba Purification Polysaccharide Sulfation Immunostimulating activity

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Identification of Anamorph Yeast of tremella aurantialba and Optimization of Medium Composition for Production of Exopolysaccharides

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过程工程学报 2012年第6期12卷 973-981页

作者：刘春卉杨秀伟俞建国马维新瞿伟菁包艳洁武文斌庄秀园 China National Institute of Standardization Beijing 100088China Dept.Natural Medicines State Key Lab.Natural and Biomimetic DrugsSch.Pharm.Sci.Peking Univ.Beijing 100191China Jiangsu Tongyuantang Bio-engineering Co. Ltd.TaixingJiangsu 225400China School of Life Science East China Normal UniversityShanghai 200062China

A yeast-like fungus strain B1 isolated from wild fungus tremella aurantialba was identified and initially characterized. Two phylogenetic trees were generated based on the sequences of large subunit ribosomal RNA gene D1/D2 regions and internal transcribed spacer (ITS) regions of related fungi, respectively. The analysis of D1/D2 regions and ITS sequences showed that fungus B1 was clustered together with T. aurantialba, T. aurantia and T. microspore in the phylogenetic trees. Both the morphological characteristic and phylogenetic analysis established that fungus B1 was one of the anamorph strains of T. aurantialba and belongs to tremella genus. A fermentation medium for exopolysaccharides (EPS) production by T. aurantialba B1 . Plackett-Burmen design was used to evaluate the effects of different components in the culture medium. Glucose and yeast extract have significant influence on the EPS production. The concentrations of two factors were optimized subsequently using central composite design and response surface analysis. The results showed that 49.2 g/L glucose and 10.4 g/L yeast extract could lead to the maximum production of EPS (4.99 g/L). The optimized medium led to a 1.5-fold enhancement of the production of EPS by T. aurantialba B1 , as compared with that without optimization.

关键词： tremella aurantialba anamorph yeast large subunit ribosomal RNA gene internal transcribed spacer exopolysaccharides central composite design

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Research on Protoplast Preparation and Regeneration Conditions of tremella aurantialba

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Agricultural Science & Technology 2012年第1期13卷 60-63,67页

作者：贺婷郭成金天津师范大学生命科学学院天津300387

[Objective] This study aimed to explore the protoplast preparation and re- generation conditions of tremella aurantialba. [Method] Optimal combination of five factors affecting the protoplast preparation and regeneration of tremella aurantialba was selected by using orthogonal experiment, including enzyme system, culture age, enzymolysis temperature, enzymolysis duration and osmotic stabilizer. [Result] The results showed that there were three release modes of tremella aurantialba proto- plasts： release from top in the early period of enzymolysis, release from the side and release in situ. The optimal protoplast preparation condition was selecting mycelium cultured in liquid medium for 3 d, for enzymolysis in mixed enzyme solu- tion containing 1% of cellulase ＋1% of snailase ＋1% of lywallzyme at 36 ℃; for 4 h, with 0.6 mol/L sucrose as osmotic stabilizer, and the obtained preparation rate had reached 1.76 ×10^7 protoplasts/ml. The optimal regeneration condition was using mycelium cultured in liquid medium for 5 d, for enzymolysis in enzyme solution con- taining 1.5% of lywallzyme at 33 ℃ for 3 h, with 0.6 mol/L sucrose as osmotic sta- bilizer, and the regeneration rate had reached 0.22%. [Conclusion] This study provid- ed valuable support for protoplast fusion, ultraviolet mutation breeding and genetic engineering research.

关键词： tremella aurantialba Protoplast Preparation Regeneration Orthogonalexperiment design

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