检索结果-南通市图书馆

Comprehensive analyses of the proteome and ubiquitome revealed mechanism of high temperature accelerating petal abscission in tree peony

引用

Horticultural Plant Journal 2024年第1期10卷 205-222页

作者： Chunying Liu Ziqi Liu Yanchao Yuan Yuxi Zhang Yifu Fang Junqiang Chen Shupeng Gai Key Laboratory of Plant Biotechnology in Shandong Province College of Life SciencesQingdao Agricultural UniversityQingdaoShandong 266109China Shandong Academy of Forestry JinanShandong 250014China

tree peony(Paeonia suffruticosa Andrews)is a well-known ornamental plant with high economic value,but the short fluorescence is a key obstacle to its ornamental value and industry *** temperature accelerates flower senescence and abscission,but the associated mechanisms are poorly *** this study,the tandem mass tag(TMT)proteome and label-free quantitative ubiquitome from tree peony cut flowers treated with 20℃for 0 h(RT0),20℃or 28℃for 60 h(RT60 or HT60)were examined based on morphological observation,***,6970 proteins and 1545 lysine ubiquitinated(Kub)sites in 844 proteins were *** residues(such as glutamate and aspartate)neighboring the Kub sites were in preference,and 36.01%of the Kub sites were located on the protein *** differentially expressed proteins(DEPs)and Kub-DEPs in HT60 vs RT60 were mainly enriched in ribosomal protein,protein biosynthesis,secondary metabolites biosynthesis,flavonoid metabolism,carbohydrate catabolism,and auxin biosynthesis and signaling revealed by GO and KEGG analysis,accompanying the increase of endogenous abscisic acid(ABA)accumulation and decrease of endogenous indoleacetic acid(IAA)***,the expression patterns of six enzymes(SAMS,ACO,YUC,CHS,ANS and PFK)putatively with Kub modifications were analyzed by proteome and real-time quantitative *** cell-free degradation assays showed PsSAMS and PsACO proteins could be degraded via the 26 S proteasome system in tree peony ***,a working model was proposed for the acceleration of flower senescence and abscission by high *** summary,all results contributed to understanding the mechanism of flower senescence induced by high temperature and prolonging fluorescence in tree peony.

关键词： tree peony Proteome Ubiquitome High temperature Flower senescence

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Genetic diversity and relatedness analysis of nine wild species of tree peony based on simple sequence repeats markers

引用

Horticultural Plant Journal 2021年第6期7卷 579-588页

作者： Yuqian Xue Rong Liu Jingqi Xue Shunli Wang Xiuxin Zhang Institute of Vegetables and Flowers Chinese Academy of Agricultural SciencesBeijing 100081China Key Laboratory of Biology and Genetic Improvement of Horticultural Crops Ministry of Agriculture and Rural AffairsBeijing 100081China

tree peony has nine wild species,but the evolutionary relationship of them is still ***,a total of 274 specimens from 22 natural populations of nine wild species were collected,and their genetic diversity and similarity was analyzed based on Simple Sequence Repeats(SSR)molecular markers.A total of 106 alleles were generated based on 20 primers and with an average of 5.3 alleles per ***’s information index(I)ranged from 0.6333 to 1.7842,and the average of Nei’s genetic diversity coefficient(H)was *** Information Content(PIC)value varied from 0.29 to 0.77,ten of these primers had high polymorphism(PIC≥0.50).All the above genetic parameters of primers reflect more rich genetic diversity information compared with other researches using SSR molecular markers to study the genetic diversity of tree peony wild *** the population level,the lowest and highest degree of genetic diversity occurred in Paeonia ludlowii-P1 and ***-P3 population,*** at species level,the genetic diversity of 9 wild peony species was as follows:***>***>***>***>***>***>***>***>***,cluster analysis at species level divided the nine wild tree peony species into two *** branch I,the closest phylogenetic relationship was found between *** and ***,followed by ***,***,and *** branch II,the closest relationship occurred between *** and ***,followed by *** and *** results supported the division of tree peonies into two subsects(Delavayanae and Vaginatae),it also supported *** and *** as independent *** results provided novel insight into the genetic diversity and phylogenetic relationship of nine wild tree peony *** will help formulate comprehensive protection measures of wild germplasm resources and select proper parents for distant hybridization in the future.

关键词： tree peony Molecular marker Genetic diversity Phylogenetic relationship

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Transcriptome Profiling of Flower Development Reveals Key Genes Mediating Yellow Formation in tree peony

引用

Phyton-International Journal of Experimental Botany 2022年第11期91卷 2505-2518页

作者： Yanzhao Zhang Chenyang Yu Xi Lu Yanwei Cheng Life Science Department Luoyang Normal UniversityLuoyang471934China

tree peony cultivars with yellow flowers are rare and valuable,but the molecular mechanism of pigment accumulation is not *** this study,the petal transcriptome of three developmental stages were sequenced to determine the differentially expressed genes(DEGs)regulating yellow flowers *** results showed that 10,842 and 12,022 DEGs were screened in stage 1 *** 2 and in stage 2 *** 3,*** analysis of flavonoid structural genes(FSGs),we found that the transcription level of DFR was very low in the three developmental *** a small group of cultivars,the DFR transcription level of red flowers was 862 times higher than that of yellow *** data suggested that the flavonoid pathway is interrupted in the later stage due to the low transcriptional level of DFR,which limits the biosynthesis of anthocyanins in yellow *** transcription levels of F3’H and FLS were upregulated from stage 1 to stage 2,while those of CHI and FLS were downregulated from stage 2 to stage *** addition,67 MYBs and 44 bHLHs showed similar transcription profiles with different members of *** results deepen our understanding of the molecular mechanism of yellow pigment accumulation in tree peony.

关键词： tree peony yellow flower transcriptome flavonoids DFR gene

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Osmotic Regulation,Antioxidant Enzyme Activities and Photosynthetic Characteristics of tree peony(Paeonia suffruticosa Andr.)in Response to High-Temperature Stress

引用

Phyton-International Journal of Experimental Botany 2023年第11期92卷 3133-3147页

作者： Zhipeng Sheng Jiasong Meng Jun Tao Daqiu Zhao College of Horticulture and Landscape Architecture Yangzhou UniversityYangzhou225009China

tree peony(Paeonia suffruticosa Andr.)is a traditional Chinese flower,which prefers cool ***,high temperature in summer in the middle and lower reaches of the Yangtze River restricts its growth and *** this study,osmotic regulation,antioxidant enzyme activities,and photosynthetic characteristics of tree peony in response to high-temperature stress were *** results showed that high-temperature stress had destroyed the cell membrane,manifested as the increased relative electrical conductivity and malondialdehyde ***,high-temperature stress led to excessive accumulation of reactive oxygen species,thereby,activating antioxidant enzyme ***,photosynthetic parameters and chlorophyll fluorescence parameters directly reflected the damage to the photosystem II reflection center under high-temperature *** addition,high-temperature stress led to stomatal closure and chloroplast *** study revealed the physiological responses of tree peony to high-temperature stress,laying a foundation for the promotion of tree peony in high-temperature areas and the improvement of high-temperature resistance.

关键词： tree peony high-temperature stress antioxidant enzyme photosynthetic characteristics

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Effects of Foliage Boron-Spraying on Seed Yields and Fatty Acid Composition of tree peony(Paeonia ostii ‘Feng Dan’)

引用

Wuhan University Journal of Natural Sciences 2018年第1期23卷 56-62页

作者： LI Chengcheng JI Wenli SUN Jiayi LI Qiaoli YANG Jingxuan WEI Shuangyu ZHANG Yanlong College of Landscape Architecture and Arts NorthwestAgriculture and Forestry University Yangling 712100 ShaanxiChina College of Forestry Northwest Agriculture and ForestryUniversity Yangling 712100 Shaanxi China

The influence of the foliage boron（B）-spaying concentration on the yield and fatty acid composition of tree peony（Paeonia ostii ＇Feng Dan＇） was explored in the experiment of 2014-2015. In this research, a statistically significant correlation was found between the mass concentration of foliage boron-spraying and tree peony seed yield. Maximum yield increment of tree peony seeds was obtained when the boron-spraying concentration was 4 g/L. The composition of fatty acid was analyzed by gas chromatography-mass spectrometry（GC-MS）, and the main components of the fatty acid composition were quantified by internal standard method. The increase in the oil extraction rate after foliage boron-spraying nutrition of different concentration was significant and ranged between 0.04% and 11.43%. Seed oil production and oil extraction rate were also increased due to the increase of seed yields. Furthermore, foliage boron-spraying had a significant effect on the content of linolenic acid and linoleic acid in seed oil.

关键词： tree peony foliage boron-spraying seed yield GC-MS fatty acid composition

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Distribution of Metabolites in Root Barks of Seven tree peony Cultivars for Quality Assessment Using NMR-based Metabolomics

引用

Chinese Herbal Medicines 2017年第1期9卷 31-41页

作者： Pei Wang Ze-ming Rong Cui-xia Ma Xin-feng Zhao Chao-ni Xiao Xiao-hui Zheng Key Laboratory of Resource Biology and Biotechnology in Western China Ministry of Education College of Life Sciences Northwest University Xi'an 710069 China

Objective To determine the distribution of metabolites in the root barks of different tree peony cultivars for quality assessment. Methods Seven tree peony phenotypic cultivars with different colors were systematically analyzed using NMR-based metabolomics. Results A total of 16 metabolites from their methanol extracts were simultaneously identified and quantified, including one primary metabolite （sucrose） and 15 secondary ones （acetophenones, phenolics, monoterpene glycosides, flavonoids, and unsaturated fatty acids）. The quantitative data indicated that sucrose （90-180 mg/g） and acetophenones （15-100 mg/g）, and non-phenolics, monoterpene glycosides, flavonoids, and unsaturated fatty acids （2-15 mg/g） were the major metabolites in these tree peony cultivars. The significantly increasing levels of paeonoside with bioactivity were observed in ＂Xiangyu＂, ＂Wujinyaohui＂, ＂Roufurong＂, ＂Yaohuang＂, ＂Zhaofen＂, ＂Doulu＂, and ＂Yingrihong＂ in order. Opposite trends in the levels of paeonoside and paeonol were observed in ＂Xiangyu＂ and ＂Yingrihong＂, suggesting that the changes of the secondary metabolites in plants were influenced by primary metabolites, such as sucrose/glucose, and the different physiological processes occurred in different tree peony cultivars. Conclusion ＂Yingrihong＂ with red flower has the highest medicine quality whereas ＂Xiangyu＂ with white flower has the worst one based on the content of paeonoside.

关键词： metabolomics NMR quality assessment tree peony

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Molecular Cloning and Expression Analysis of Cryptochrome Gene Ps CRY2in tree peony

引用

Horticultural Plant Journal 2016年第6期2卷 357-362页

作者： REN Xiuxia WANG Shunli XUE Jingqi ZHU Fuyong LIU Chuanjiao ZHANG Xiuxin Key Laboratory of Biology and Genetic Improvement of Horticultural Crops Ministry of Agriculture Institute of Vegetables and Flowers Chinese Academy of Agricultural Science

Cryptochromes are blue/ultraviolet-A(UV-A) light receptors involved in regulating various aspects of plant growth and *** of the structure and functions of cryptochromes in plants have largely focused on herbaceous plants. However, few data on the function of CRY2 are available in woody plants. In this study, a cryptochrome 2(CRY2) gene was isolated from Paeonia suffruticosa by Reverse Transcription Polymerase Chain Reaction(RT-PCR). Sequence alignment and motif analysis showed that the deduced amino acids contained a PHR domain near the amino terminus and a CCT domain near the carboxy terminus. Ps CRY2 showed high identity with At CRY2 of *** analysis indicated that it was closely related to Citrus sinensis. Gene expression analysis revealed that the highest expression levels of Ps CRY2 occurred in the bud and seed embryo of P. suffruticosa, followed by the roots, stems, and leaves. Ps CRY2 was upregulated during the entire process of bud differentiation, whereas this was downregulated during the early stage of bud development and upregulated in the middle and late stages. The highest level of Ps CRY2 expression was observed in the big bell-like flower buds. These results suggested that Ps CRY2 plays an important role in both bud differentiation and bud development. The expression patterns of Ps CRY2 in the buds of ‘Luoyanghong' and ‘Qiufa1' were similar, whereas that in the buds of ‘Qiufa 1' was significantly higher than in the buds of ‘Luoyanghong'. The buds of plants subjected to different photoperiod treatments exhibited variations in Ps CRY2 expression patterns. The expression of Ps CRY2 decreased during bud sprouting and in the small bell-like flower buds that were subjected to short-day photoperiod compared to that observed under long-day photoperiod.

关键词： tree peony photoperiod pathway cryptochromes Ps CRY2 q RT-PCR

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Establishment of highly efficient plant regeneration of Paeonia ostii‘Fengdan’through optimization of callus,adventitious shoot,and rooting induction

引用

Horticultural Plant Journal 2022年第6期8卷 777-786页

作者： Rong Liu Yuqian Xue Huiting Ci Jie Gao Shunli Wang Xiuxin Zhang Key Laboratory of Biology and Genetic Improvement of Horticultural Crops Ministry of Agriculture and Rural Affairs ChinaInstitute of Vegetables and FlowersChinese Academy of Agricultural SciencesInstitute of PeonyChinese Academy of Agricultural SciencesBeijing 100081China

tree peony is a famous ornamental plant,while the low propagation rate is the main hurdles hindering the industry *** now,the highly efficient regeneration system for tree peony is not *** this study,using Paeonia ostii’Fengdan’mature embryos,the effects of variations in inoculation method,initiating culture,adventitious shoot induction,rooting media,plant growth regulators(PGRs),and a nonconventional PGR(plant extracts)on regeneration from explants were *** embryo cultures,embryonic callus induction rate of 1/4 embryos was the highest among those of embryos with other three technical treatments(whole embryos,1/2 embryos,and pieces of embryos).The woody plant medium(WPM)containing 1.0 mg·L^(-1)6-BA,0.5 mg·L^(-1)GA3,30.0 g·L^(-1)sucrose,and 3.0 g·L^(-1)phytagel significantly improved shoot induction and multiplication.3.0 mg·L^(-1)plant extracts promoted hypocotyl germination,rooting,and root growth,in direct embryo culture,and a combination of 3.0 mg·L^(-1)plant extracts+2.0 mg·L^(-1)IBA+1.5 mg·L^(-1)IAA produced optimal rooting induction rate for multiple shoots in direct embryo culture and indirect somatic *** the three in vitro micropropagation methods,the highest shoot proliferation coefficient(5.4±0.2)was obtained with indirect somatic ***,the propagation ability of shoots remains high,even when culture propagation was continued for more than two ***,a reliable system for plant regeneration from mature embryos derived from ***’Fengdan’callus and two direct embryo culture systems have been *** novel regeneration system could facilitate uniform seedling production.

关键词： tree peony Mature embryos Plant extracts Shoot induction Rooting Plant regeneration

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Transcriptome Analysis and Genes Function Verification of Root Development of Paeonia suffruticosa under Sandy Loam Cultivation

引用

Phyton-International Journal of Experimental Botany 2022年第12期91卷 2791-2812页

作者： Yinglong Song Wenqian Shang Zheng Wang Songlin He Xinya Meng Liyun Shi Yuxiao Shen Dan He Xueyuan Lou Yuke Sun College of Landscape Architecture and Art Henan Agricultural UniversityZhengzhou450002China School of Horticulture Landscape Architecture Henan Institute of Science and TechnologyXinxiang453003China College of Forestry Hainan UniversityHaikou570228China

Relatively poor in vitro rooting has limited the large-scale commercial production of tree *** this study,on the basis of transcriptome sequencing,differentially expressed genes and the associated metabolic pathways were identified in tree peony roots at different stages of root formation under sandy loam cultivation.A total of 31.63 Gb raw data were generated and 120,188 unigenes(mean length of 911.98 bp)were annotated according to six databases(NR,NT,GO,KEGG,COG,and Swiss-Prot).Analyses of the ungerminated root primordium period,induced root primordium period,and root formation period detected 8,232,6,907,and 10,687 differentially expressed genes related to 133,132,and 133 metabolic pathways,*** significantly differentially expressed genes(Unigene13430_All and ***1_All)were associated with the auxin *** full-length Unigene13430_All coding sequence(843 bp)encoded 280 amino acids,whereas the full-length ***1_All coding sequence(1,470 bp)encoded 489 amino ***13430_All and ***1_All were identified as IAA gene family members and were respectively named PsIAA27 and *** qRT-PCR analysis and functional verification indicated that the expressions of PsARF19 and PsIAA27 in tree peony seedlings,cuttings and grafted seedlings were significant ***19 promoted root development,it might be a regulatory gene related to the formation of tree peony roots,while PsIAA27 inhibited lateral root development,and it might be involved in controlling auxin sensitivity during root *** results of this study may form the basis of future investigations on the mechanism mediating peony root *** transcriptome data will be an excellent resource for researchers interested in characterizing the rooting-related tree peony genes.

关键词： tree peony rooting RNA-seq cuttage graft functional verification qRT-PCR

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

安徽药用牡丹炭疽病病原菌鉴定

引用

植物病理学报 2023年第1期53卷 137-140页

作者：杨红梅孙振鑫王铁霖黄璐琦张茹琴鄢洪海青岛农业大学植物医学学院青岛266109 中国中医科学院北京100700

牡丹(Paeonia suffruticosa Andr.)是芍药科植物,为多年生落叶灌木,具有很高的观赏和药用价值。因其风姿华丽、色彩艳丽、品种繁多、深受大家喜爱,是中国传统名花和世界著名花卉之一。其根皮也被称为丹皮,具有抗炎、抑制血小板、止痛、... 详细信息

牡丹(Paeonia suffruticosa Andr.)是芍药科植物,为多年生落叶灌木,具有很高的观赏和药用价值。因其风姿华丽、色彩艳丽、品种繁多、深受大家喜爱,是中国传统名花和世界著名花卉之一。其根皮也被称为丹皮,具有抗炎、抑制血小板、止痛、清热等作用,是六味地黄丸等著名中成药的主要配方,也是饮片中的常用品[1-2]。近年来,随着牡丹种植面积的不断扩大,造成牡丹病害的大面积发生,已给牡丹生产带来严重影响。

关键词： tree peony leaf disease identification of pathogenic fungus

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：