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Rice Chitin Receptor OsCEBiP Is Not a transmembrane protein but Targets the Plasma Membrane via a GPI Anchor

Molecular Plant 2017年第5期10卷 767-770页

作者： Gong, Ben-Qiang Xue, Jiao Zhang, Nannan Xu, Lahong Yao, Xinran Yang, Qiu-Jiao Yu, Yang Wang, Hong-Bin Zhang, Dandan Li, Jian-Feng Sun Yat Sen Univ Sch Life Sci State Key Lab Biocontrol Guangzhou 510275 Guangdong Peoples R China Sun Yat Sen Univ Sch Life Sci Guangdong Prov Key Lab Plant Resources Guangzhou 510275 Guangdong Peoples R China

Dear Editor,Plants are sessile organisms that are constantly challenged by microbial pathogens from the habitat. The key for plants to survive is to perceive pathogens in a timely and precise manner and subsequently l... 详细信息

关键词： Rice Chitin Receptor OsCEBiP transmembrane protein

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transmembrane protein VSD is required for axon crossing for the formation of central complex

Transmembrane protein VSD is required for axon crossing for ...

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中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Xue-Bo CHENG,Hai-Yun GONG,Zhe-Feng GONG,Li LIU~* State Key Laboratory of Brain and Cognitive Science,Institute of Biophysics,Chinese Academy of Sciences,Beijing 100101,China

＜正＞Objective Neuronal connections between the hemispheres are considered important for organisms to organize information to adapt to variable *** central complex split flies,the locomotor activity and the visual learning and memory are *** midline crossing is an event that requires many components to regulate each *** study is aimed to find the genes that affect the formation of central *** Histochemistry with antibody nc82 was used to observe the shape of central *** blot with anti- vsd specific antibodies were used to study the characteristic of the ***（1） Nonsense mutation in the extracellular domain of VSD contributed to the central complex defect.（2） VSD can be cleaved by an unknown protease and release the intracellular domain from the membrane.（3） Overexpression the extracellular domain of NRG can partially rescue the mutant phenotype caused by knockdown of *** transmembrane protein VSD （visual system disorganization） is essential for the formation of the central complex and NRG cooperates with VSD to regulate the axon midline crossing.

关键词： Drosophila melanogaster,central complex histochemistry transmembrane protein

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Prediction of transmembrane helical segments in transmembrane proteins based on wavelet transform

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Journal of Shanghai University(English Edition) 2006年第4期10卷 308-318页

作者：于彬孟宪花刘海军王翼飞 Department of Mathematics College of Sciences Shanghai University Shanghai 200444 P.R. China College of Mathematics and Physics Qingdao University of Science and Technology Qingdao 266061 P.R. China

Tmnsmembrane（TM） protein plays an important role in the life activity of the cells, and the prediction of transmembrane helical segments （TMHs） is an important subject in the bioinformatics research. Thus far, several prediction methods have been reported, but there are some deficiencies in prediction accuracy and adaptability in these methods. In this paper, a method based on discrete wavelet transform （DWT） was developed to predict the TMHs. Two sets of test data sets containing total 60 protein sequences were utilized to access the effect of the method. Compared with the prediction results of TMHMM2.0 and MEMSAT, the obtained results indicate that the presented method has high prediction accuracy.

关键词： transmembrane protein transmembrane helical segments (TMHs) hydrophobicity discrete wavelet transform.

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Discrimination of intra/extracellular sidedness segments of transmembrane proteins

Discrimination of intra/extracellular sidedness segments of ...

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第十一次中国生物物理学术大会暨第九届全国会员代表大会

作者： Wei Chen,Liao-fu Luo School of Physical Science and Technology,Inner Mongolia University,Hohhot 010021,China

＜正＞Topology of the transmembrane protein is closely related to its ***,it is desiderated to depict the topology of transmembrane *** this work,an effective approach of

关键词： transmembrane protein topology increment of diversity quadratic discriminant analysis

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AtTMEM18 plays important roles in pollen tube and vegetative growth in Arabidopsis

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Journal of Integrative Plant Biology 2016年第7期58卷 679-692页

作者： Xiao-Ying Dou Ke-Zhen Yang Zhao-Xia Ma Li-Qun Chen Xue-Qin Zhang Jin-Rong Bai De Ye Beijing Radiation Center 12 Haidian NanluBeijing 100875China State Key Laboratory of Plant Physiology and Biochemistry College of Biological SciencesChina Agricultural University2 Yuanmingyuan XiluBeijing 100193China.

In flowering plants, pollen tube growth is essential for delivery of male gametes into the female gametophyte or embryo sac for double fertilization. Although many genes have been identified as being involved in the process, the molecular mechanisms of pollen tube growth remains poorly understood. in this study, we identified that the Arabidopsis transmembrane protein 18 （AtTMEM18） gene played important roles in pollen tube growth. The AtTMEM18 shares a high similarity with the transmembrane 18 proteins （TMEM18s） that are conserved in most eukaryotes and may play important roles in obesity in humans. Mutation in the AtTMEM18 by a Ds insertion caused abnormal callose deposition in the pollen grains and had a significant impact on pollen germination and pollen tube growth. AtTMEM18 is expressed in pollen grains, pollen tubes, root tips and other vegetative tissues. The pollen-rescued assays showed that the mutation in AtTMEM18 also caused defects in roots, stems, leaves and transmitting tracts. AtTMEM18-GFP was located around the nuclei. Genetic assays demonstrated that the localization of AtTMEM18 around the nuclei in the generative cells of pollen grains was essential for the male ***, expression of the rice TM EM18-homologous protein （OsTMEM18） driven by LAT52 promoter could recover the fertility of the Arabidopsis attmem18 mutant. These results suggested that the TMEM18 is important for plant growth in Arabidopsis.

关键词： Arabidopsis male gametophyte plant pollen TMEM18 transmembrane protein

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Selective aggregation of membrane proteins by membrane-mediated interactions

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Science China Chemistry 2014年第12期57卷 1683-1689页

作者： LI Shuang Yang ZHANG Xian Ren WANG Wen Chuan School of Food and Chemical Engineering Beijing Technology and Business University State Key Laboratory of Organic-Inorganic Composites Beijing University of Chemical Technology

There are different types of membrane proteins in a cellular membrane,and most of them must correctly assemble into appropriate clusters for their cellular *** this work,we suggest a physical mechanism for selective aggregation of different membrane proteins without specific protein-protein attraction by dissipative particle dynamics method.A membrane-mediated interaction may result in different protein clusters with ideal mixing,nonideal mixing and demixing of different types of membrane proteins,depending on the extent of the similarity of membrane deformations by those proteins.

关键词： selective aggregation anchored protein transmembrane protein dissipative particle dynamics(DPD)

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OsHT, a Rice Gene Encoding for a Plasma-Membrane Localized Histidine Transporter

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Journal of Integrative Plant Biology 2005年第1期47卷 92-99页

作者： DiLIU WeiGONG YongBAI Jing-ChuLUO Yu-XianZHU StateKeyLaboratoryofproteinEngineeringandPlantGeneticEngineering PekingUniversityBeijing100871China

: Using a degenerative probe designed according to the most conservative region of a known Lys- and His-specific amino acid transporter (LHT1) from Arabidopsis, we isolated a full-length cDNA named OxHT(histidine transporter of Oryza sativa L.) by screening the rice cDNA library. The cDNA is 1.3 kb in length and the open reading frame encodes for a 441 amino acid protein with a calculated molecular mass of 49 kDa. Multiple sequence alignments showed that OsHT shares a high degree of sequence conservation at the deduced amino acid level with the Arabidopsis LHT1 and six putative lysine and histidine transporters. Computational analysis indicated that OsHT is an integral membrane protein with 11 putative transmembrane helices. This was confirmed by the transient expression assay because the OsHT-GFP fusion protein was, indeed, localized mainly in the plasma membrane of onion epidermal cells. Functional complementation experiments demonstrated that OsHT was able to work as a histidine transporter in Saccharomyces cerevisiae, suggesting that OsHT is a gene that encodes for a histidine transporter from rice. This is the first time that an LHT-type amino acid transporter gene has been cloned from higher plants other than Arabidopsis.

关键词： histidine transporter LHT1 rice transmembrane protein

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Integral nuclear membrane protein Nurim plays a role in suppression of apoptosis

Integral nuclear membrane protein Nurim plays a role in supp...

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细胞—生命的基础——中国细胞生物学学会2013年全国学术大会·武汉

作者： Chen H Chen K Chen J Cheng H Zhou R Department of Genetics College of Life Sciences Wuhan University Department of Oncology Zhongnan HospitalWuhan University

As an essential component of eukaryotic cells,nuclear envelope(NE) plays a crucial role in many physiological *** few membrane proteins of the NE have been functionally characterized. To determine whether the inner nu... 详细信息

关键词： nuclear envelope transmembrane protein apoptosis cancer

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