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Expression of Hepatitis B Surface Antigen Gene in Ginseng cells

Chemical Research in Chinese Universities 2009年第5期25卷 695-698页

作者： YU Hai-peng XUE Yan AN Wei LIU Dan HAO Shu-mei SHENG Jun College of Life Science Jilin University Changchun 130012 P R. China Changchun Institute of Biological Products Changchun 130062 P R. China Changchun University of Science and Technology Changchun 130022 13. R. China Organ Transplantation Department of First Hospital Jilin University Changchun 130021 P R. China

The recombinant plasmid pBIBSa containing the HBsAg DNA fragment was transferred into Agrobacterium tumefaciens strain LBA4404 directly. Ginseng cells were transfected with A. tumefaciens carrying pBIBSa and the ginseng cell lines carrying HBsAg-S gene were obtained. The presence of target gene in the transfect cells was confirmed by PCR and RT-PCR. A clear band at the site of 700 bp was observed by agarose electrophoresis analysis of the samples containing the target gene. HBsAg expressed by the transgenic ginseng cells was detected by Western blot. Maximum expression levels of 184 ng HBsAg/g FW and 0. 009% of the total soluble proteins were observed by ELISA. HBsAg in ginseng cells was located both on the cell membrane and in the nuclei.

关键词： transgenic cell HBsAg Ginseng

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NON-DESTRUCTIVE ANALYSIS OF THE NUCLEI OF transgenic LIVING cellS USING LASER TWEEZERS AND NEAR-INFRARED RAMAN SPECTROSCOPIC TECHNIQUE

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Genomics, Proteomics & Bioinformatics 2005年第3期3卷 169-178页

作者： Wei Tang Ronald J. Newton Chang-An Xie Yong-Qing Li Nicki Whitley Department of Biology Howell Science Complex East Carolina UniversityGreenville NC 27858-4353 USA. Department of Physics Howell Science Complex East Carolina University Greenville NC 27858-4353 USA.

transgenic cell lines of loblolly pine （Pinus taeda L.） were analyzed by a compact laser-tweezers-Raman-spectroscopy （LTRS） system in this investigation. A low power diode laser at 785 nm was used for both laser optical trapping of single transgenic cells and excitation for near-infrared Raman spectroscopy of the nuclei of synchronized cells, which were treated as single organic particles, at the S-phase of the cell cycle. transgenic living cells with gfp and uidA genes were used as biological samples to test this LTRS technique. As expected, different Raman spectra were observed from the tested biological samples. This technique provides a high sensitivity and enables real-time spectroscopic measurements of transgenic cell lines. It could be a valuable tool for the study of the fundamental cell and molecular biological process by trapping single nucleus and by providing a wealth of molecular information about the nuclei of cells.

关键词： transgenic cell laser optical trapping near-infrared Raman spectroscopy biological sample

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