检索结果-南通市图书馆

transgenic Expression of the Recombinant Phytase in rice (Oryza sativa)

在线全文

学校读者我要写书评

暂无评论

rice science 2006年第2期13卷 79-84页

作者： LIU Qiao-quan LI Qian-feng JIANG Li ZHANG Da-jiang WANG Hong-mei Gu Ming-hong YAO Quan-hong Key Laboratory of Plant Functional Genomics of the Ministry of Education College of Bioscience and Biotechnoiogy Yangzhou University Yangzhou 225009 China Biotechnology Center Shanghai Academy of Agricultural Sciences Shanghai 201106 China

In most of the cereal crop, phytic acid is the main storage form of phosphorus, which can decrease the bioavailability of phosphate. transgenic expression of phytase is regarded as an efficient way to release phosphate from phytate in transgenic plants. In this study, a plant expression vector, containing the recombinant phytase gene driven by the maize ubiquitin （Ubi） promoter was constructed and introduced into an elite rice variety via Agrobacterium-mediated transformation. During the experiment, a total of 15 independent transgenic rice lines were regenerated. The results of PCR and Southern blot indicated that the target gene was integrated into the genome of transgenic rice plants. Moreover, the RT-PCR analysis of total RNAs extracted from the immature seeds of several transgenic lines showed that the recombinant phytase gene could be normally expressed. The inorganic phosphorus content, both in the mature seeds and the leaf was significantly higher in the transgenic plants than in the untransformed wild type.

关键词： transgenic rice recombinant phytase inorganic phosphorus content nutritional quality

Functional and numerical responses of Cyrtorhinus lividipennis to eggs of Nilaparvata lugens are not affected by genetically modified herbicide-tolerant rice

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2015年第10期14卷 2019-2026页

作者： JIANG Xian-bin HUANG Qian LING Yan CHEN Yu-chong XIAO Guo-ying HUANG Suo-sheng WU Bi-qiu HUANG Feng-kuan CAI Jian-he LONG Li-ping Guangxi Key Laboratory of rice Genetics and Breeding Rice Research Institute Guangxi Academy of Agricultural Sciences Plant Protection Research Institute Guangxi Academy of Agricultural Sciences Guangxi Academy of Agricultural Sciences Institute of Subtropical Agriculture Chinese Academy of Sciences

To safely and sustainably utilize genetic breeding techniques for crop production, greater understanding of the potential effects of genetically modified herbicide-tolerant（GMHT） crops on the ecological functions of predators is required. In the laboratory, we examined the functional and numerical responses of Cyrtorhinus lividipennis Reuter to eggs of brown planthopper（BPH）, Nilaparvata lugens（St？l）, which were reared on GMHT rice Bar68-1; the untransformed parental cultivar, D68; or a BPH-susceptive rice variety, Taichung Native 1. All stages of nymphs and female adult of C. lividipennis, either on GMHT rice or control plants, exhibited typical type II functional responses when fed on BPH eggs; the attacking rate and handling time of C. lividipennis on GMHT rice Bar68-1 was not significantly different from that on D68. The numerical responses of C. lividipennis on GMHT rice or controls fit Beddington＇s model; there were no significant differences in the parameters of numerical responses between GMHT rice Bar68-1 and D68. The results indicated that the functional and numerical responses of C. lividipennis to BPH eggs are not affected by GMHT rice Bar68-1.

关键词： transgenic rice green mirid bug brown planthopper natural enemy non-target organisms predator herbicide resistant safety assessm

Characterization of a Novel rice Metallothionein Gene Promoter:Its Tissue Specificity and Heavy Metal Responsiveness

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2010年第10期52卷 914-924页

作者： Chun-Juan Dong Yun Wang Shi-Shi Yu Jin-Yuan Liu Laboratory of Molecular Biology and MOE Laboratory of Protein Science School of Life Sciences Tsinghua University Beijing 100084China

The rice （Oryza sativa L.） metallothionein gene OsMT-I-4b has previously been identified as a type I MT gene. To elucidate the regulatory mechanism involved in its tissue specificity and abiotic induction, we isolated a 1 730 bp fragment of the OsMT-I-4b promoter region. Histochemical /^-glucuronidase （GUS） staining indicated a precise spacial and temporal expression pattern in transgenic Arabidopsis. Higher GUS activity was detected in the roots and the buds of flower stigmas, and relatively lower GUS staining in the shoots was restricted to the trichomes and hydathodes of leaves. No activity was observed in the stems and seeds. Additionally, in the root of transgenic plants, the promoter activity was highly upregulated by various environmental signals, such as abscisic acid, drought, dark, and heavy metals including Cu2＋, Zn2~, Pb2~ and Al3＋. Slight induction was observed in transgenic seedlings under salinity stress, or when treated with Co2＋ and Cd2＋. Promoter analysis of 5＇-deletions revealed that the region -583/-1 was sufficient to drive strong GUS expression in the roots but not in the shoots. Furthermore, deletion analysis indicated important promoter regions containing different metal- responsive c/s-elements that were responsible for responding to different heavy metals. Collectively, these findings provided important insight into the transcriptional regulation mechanisms of the OsMT-I- 4b promoter, and the results also gave us some implications for the potential application of this promoter in plant genetic engineering.

关键词： PLANT gene expression PLANT genetics METALLOTHIONEIN PROMOTERS (Genetics) HEAVY metals PLANT genetic engineering transgenic rice ARABIDOPSIS PLANT cells & tissues GENETIC regulation in plants

Unintended Changes in Genetically Modified rice Expressing the Lysine-Rich Fusion Protein Gene Revealed by a Proteomics Approach

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2013年第11期12卷 2013-2021页

作者： ZHAO Xiang-xiang TANG Tang LIU Fu-xia LU Chang-li HU Xiao-lan JI Li-lian LIU Qiao-quan Jiangsu Key Laboratory for Eco-Agricultural Biotechnology Around Hongze Lake Huaiyin Normal University Key Laboratories of Crop Genetics and Physiology of the Jiangsu Province and Plant Functional Genomics Ministry of Education/Yangzhou University

Development of new technologies for evaluating genetically modiifed （GM） crops has revealed that there are unintended insertions and expression changes in GM crops. Proifling techniques are non-targeted approaches and are capable of detecting more unintended changes in GM crops. Here, we report the application of a comparative proteomic approach to investigate the protein proifle differences between a GM rice line, which has a lysine-rich protein gene, and its non-transgenic parental line. Proteome analysis by two-dimensional gel electrophoresis （2-DE） and mass spectrum analysis of the seeds identiifed 22 differentially expressed protein spots. Apart from a number of glutelins that were detected as targeted proteins in the GM line, the majority of the other changed proteins were involved in carbohydrate metabolism, protein synthesis and stress responses. These results indicated that the altered proteins were not associated with plant allergens or toxicity.

关键词： transgenic rice proteome profiling techniques unintended changes two-dimensional gel electrophoresis

Comparative Study on Growth Performance of transgenic(Over-Expressed OsNHX1) and Wild-Type Nipponbare under Different Salinity Regimes

在线全文

学校读者我要写书评

暂无评论

rice science 2015年第6期22卷 275-282页

作者： Nurul Kahrani ISHAK Zohrah SULAIMAN Kushan U.TENNAKOON Environmental and Life Sciences Faculty of ScienceUniversity Brunei Darussalam Institut Teknologi Brunei

transgenic Nipponbare which over-expressed a Na＋/H~ antiporter gene OsNHX1 was used to compare its growth performance, water status and photosynthetic efficiency with its wild type under varying salinity regimes. Chlorophyll content, quantum yield and photosynthetic rate were measured to assess the impact of salinity stress on photosynthetic efficiency for transgenic and wild-type Nipponbare. Effects of salinity on water status and gas exchange to both lines were studied by measuring water use efficiency, instantaneous transpiration rate and stomatal conductance. Dry shoot weight and leaf area were determined after three months of growth to assess the impacts of salinity on the growth of those two lines. Our study showed that both lines were affected by salinity stress, however, the transgenic line showed higher photosynthetic efficiency, better utilization of water, and better growth due to low transpiration rate and stomatal conductance. Reduction of photosynthetic efficiency exhibited by the wild-type Nipponbare was correlated to its poor growth under salinity stress.

关键词： growth performance salinity stress Na+/H+ antiporter gene OsNHX1 transgenic rice photosynthetic efficiency water status

The role of tocopherol cyclase in salt stress tolerance of rice(Oryza sativa)

在线全文

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2011年第2期54卷 181-188页

作者： OUYANG ShouQiang HE SiJie LIU Peng ZHANG WanKe ZHANG JinSong CHEN ShouYi State Key Laboratory of Plant Genomics and National Centre for Plant Gene Research (Beijing) Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China Graduate School of the Chinese Academy of Sciences Beijing 100049 China

Tocopherols synthesized exclusively by photosynthetic organisms are major antioxidants in *** plants,tocopherol cyclase(TC/VTE1) catalyzes the conversion of 2,3-dimethyl-5-phytyl-1,4-benzoquinone(DMPBQ) to γ-*** the present study,OsVTE1,which encodes a rice tocopherol cyclase ortholog,was cloned and ***1 was induced significantly by abiotic stresses such as high salt,H2O2,drought,cold and by the plant hormones ABA and salicylic *** tissue-specific expression pattern and OsVTE1-promoter GUS activity assay showed that OsVTE1 was mainly expressed in the leaf,and also could be detected in the root,stem and *** with control plants,transgenic plants with OsVTE1 RNA interference(OsVTE1-RNAi) were more sensitive to salt stress whereas,in contrast,transgenic plants overexpressing OsVTE1(OsVTE1-OX) showed higher tolerance to salt *** DAB in vivo staining showed that OsVTE1-OX plants accumulated less H2O2 than did control plants.

关键词： rice tocopherol tocopherol cyclase(TC/VTE1) transgenic rice abiotic stress tolerance

Transgene Flow from Glufosinate-Resistant rice to Improved and Weedy rice in China

在线全文

学校读者我要写书评

暂无评论

rice science 2014年第5期21卷 271-281页

作者： LU Yong-liang Nilda R.BURGOS WANG Wei-xia YU Liu-qing Center of rice Cultivation Technology Research China National Rice Research Institute Department of Crop Soil and Environmental Sciences University of ArkansasFayetteville 72704 AR USA

The development of transgenic rice with novel traits in China can increase rice productivity, but transgene flow to improved or weedy rice has become a major concern. We aimed to evaluate the potential maximum frequencies of transgene flow from glufosinate-resistant rice to improved rice cultivars and weedy rice. Treatments were arranged in randomized complete blocks with three replicates. Experiments were conducted between 2009 and 2010 at the Center for Environmental Safety Supervision and Inspection for Genetically Modified Plants, China National rice Research Institute, Hangzhou, China. Glufosinate-resistant japonica rice 99-1 was the pollen donor. The pollen recipients were two inbred japonica rice （Chunjiang 016 and Xiushui 09）, two inbred indica rice （Zhongzu 14 and Zhongzao 22）, two indica hybrid rice （Zhongzheyou 1 and Guodao 1）, and one weedy indica rice （Taizhou weedy rice）. The offspring of recipients were planted in the field and sprayed with a commercial dose of glufosinate. Leaf tissues of survivors were analyzed by polymerase chain reaction to detect the presence of the transgene. The frequency of gene flow ranged from 0 to 0.488%. In 2009, the order of gene flow frequency was as follows： weedy rice 〉 Chunjiang 016 〉 Xiushui 09 and Zhongzu 14 〉 Guodao 1, Zhongzheyou 1 and Zhongzao 22. Gene flow frequencies were generally higher in 2009 than in 2010, but did not differ significantly among rice materials. Gene flow frequency was the highest in weedy rice followed by the inbred japonica rice. The risk of gene flow differed significantly between years and year-to-year variance could mask risk differences among pollen recipients. Gene flow was generally lesser in taller pollen recipients than in shorter ones, but plant height only accounted for about 30% of variation in gene flow. When flowering synchrony was maximized, as in this study, low frequencies of gene flow occurred from herbicide-resistant japonica rice to other cultivars and weedy rice. Average

关键词： gene flow hybrid rice japonica rice indica rice transgenic rice weedy rice glufosinate-resistance

Indication of Genetic Linkage Map for Sunflower(Helianthus annuus L.) by SSR Markers

在线全文

学校读者我要写书评

暂无评论

Agricultural Science & Technology 2012年第12期13卷 2489-2495,2495页

作者：黄先群 Genzbitelle L. Fabre F. Saraffi A. 贵州省生物技术研究所贵州省农业生物技术重点实验室贵州贵阳550006 法国图鲁兹国立综合研究院生物技术和作物改良实验室 INP-ENSATPole de Biotechnologie Végétale

In order to investigate the possibility and efficiency of exogenous gene spread in nature and potential ecological risk of transgenic rice, as well as analyze the effect of exogenous Bt gene insertion on ecological fitness of transgenic rice plants, a experiment was carried out with three insect-resistant Bt transgenic rice cultivars Bt63, R1 and R2 and one conventional rice line 11-838 as experimental materials, the insect-resistant transgenic and non-transgenic rice plants were inter- cropped pair-wisely under high and low insect-infestation pressures, and the vegeta- tive growth, seed-setting and the resistance to rice stem borers were compared be- tween transgenic and non-transgenic lines. According to the experimental results, both the tiller number and fresh weight of Bt transgenic rice plants under low insect- infestation pressure showed no significant differences compared with the control, but the plant height, spike length and spike weight were all lower than those of non- transgenic rice plant, and Bt63 and R2 were significantly different compared with the control. On the contrary, under high insect-infestation pressure, the tiller number, spike length and spike weight of three Bt transgenic rice cultivars were significantly higher than those of the control, while the plant height showed different fitness ef- fects among various transgenic rice cultivars, which might be related to the charac- teristics of the receptive cultivars. The individual filled grain number and 1 000-grain weight of three transgenic rice cultivars showed no significant difference compared with the control under two different insect-infestation pressures, suggesting that the effect of exogenous Bt gene on seed setting was not significant. Under insect-infes- tation pressure, the resistance of three Bt transgenic rice cultivars against rice stem borer was significantly superior to non-transgenic rice, indicating that the effect of exogenous Bt gene on insect resistance of receptive pl

关键词： Ecological fitness Insect-resistance transgenic rice Insect-infestation pressure Gene flow Ecological risk

同方期刊数据库

Breeding for Parents of Hybrid rice with Maize pepc Gene

在线全文

学校读者我要写书评

暂无评论

Agricultural Sciences in China 2002年第10期1卷 1067-1073页

作者： WANG De-zheng, JIAO De-mao, WU Shuang, LI Xia, LI Li, CHI Wei, WANG Shou-hai, LI Cheng-quan, LUO Yan-chang and WANG Xiu-feng(rice Research Institute , Anhui Academy of Agricultural Sciences , Hefei 230031 , P. R . China Institute of Agrobiological Genetics and Physiology, Jiangsu Academy of Agricultural Sciences , Nanjing 210014 , P. R . China) rice Research Institute Anhui Academy of Agricultural SciencesHe fei 230031P.R.China Institute of Agrobiological Genetics and Physiology Jiangsu Academy of Agricultural Sciences Nanjing 210014 P.R.China

The results of the investigation on transgenic rice with maize C4-specific phosphoenolpyruvate carboxylase (pepc) gene showed that the transgenic rice plants with the maize pepc gene expressed at high level and the maize PEPC expression was inherited in the progenies in a Mendelian manner. The transgenic plants had PEPC activity of more than 10-fold higher than untransformed plants. As compared with untransformed plants, the panicle per plant, spikelet per panicle, 1000-grain weight and grain-weight per plant for transgenic plants increased by 14.9 % , 5.7%, 1.3 % and 13.9 %, respectively. By crossing the maize pepc gene was incorporated into the parents of hybrid rice, which were the photo-sensitive genie male sterile (PGMS) lines of two-line hybrid rice such as Peiai64s, 7001s, 2302s, 2304s and 2306s-1, and the BT type of cytoplas-mic male sterile (CMS) line of three-line hybrid rice such as Shuangjiu A, and restorer lines 5129, Wanjing97 in the spring of 1998. The following progresses were made: (1) The inheritance of the high-level expression of the maize PEPC was stable in different genetic background of rice; (2) PEPC activity of  hybrid was the mean of the two parents. Its saturated photosynthetic rate (Pn) rose to 50 % higher than that of the receptor parent. These results demonstrated that it is possible to increase the vigor of the rice plant by transgenic approach with maize pepc gene; (3) The activity of PEPC in leaf could be considered as the major physiological index because the correlation coefficient between PEPC activity and Pn was 0.6470* * ; (4) We have developed three rice lines with maize pepc gene; (5) The selection method of high photosynthetic efficiency rice has been established, which includes soaking seeds into solution of hygromycin phosphotransferase to germinate, tracing the pepc gene by PCR analysis, evaluating the performance of the rice plants in the field and examining PEPC activities and Pn of rice plants with maize pepc gene.

关键词： transgenic rice Maize pepc gene Breeding High photosynthetic efficiency Hybrid rice