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Using transcriptomics to reveal the molecular mechanism of higher alcohol metabolism in Saccharomyces cerevisiae

Food Bioscience 2023年第1期51卷 210-219页

作者： Danyao Cui Ling Liu Xiaoyuan Zhang Liangcai Lin Xin Li Ting Cheng Chunhui Wei Yu Zhang Zheng Zhou Wei Li Cuiying Zhang Key Laboratory of Industrial Fermentation Microbiology Ministry of EducationTianjin Key Laboratory of Industrial MicrobiologyCollege of BiotechnologyTianjin University of Science and TechnologyTianjin300457PR China Shandong Acad Pharmaceut Sci Natl Local Joint Engn Lab Polysaccharide DrugsKey Lab Biopharmaceut Engn Lab PolysaccharidJinan250101PR China Liquor Making Biological Technology and Application of Key Laboratory of Sichuan Province Sichuan643002PR China

High alcohols are important flavor compounds in alcoholic beverages,but high concentration of high alcohols is harmful to human *** alcohol synthesis pathways in brewing microorganisms have been investigated,but the interactions between key genes remain to be explored,especially in industrial strains of Saccharomyces *** PDC1 gene was considered to be the main encoding gene ofα-keto acid decarboxylase,and its deletion resulted in a 92.23%increase in isobutanol production and a 14.89%decrease in isoamyl alcohol *** sequencing was used to explore the effects of PDC1 gene deletion on global gene transcription levels,and deletion strategies were utilized to verify the effects of differential genes on higher alcohol *** of differential gene HMLALPHA2 increased isobutanol production by 26.23%,and decreased isoamyl alcohol and 2-phenylethanol production by 30.31%and 22.35%,*** THI2,THI4,THI20 genes were proved to be related to n-propanol *** addition,HMRA2 and SIR3 genes were found to influence isoamyl alcohol synthesis pathways,and their deletion increased isoamyl alcohol production by 25.18%and 21.76%.Our discovery of new target genes is useful for elucidating the molecular mechanisms of higher alcohols and the construction of novel low-producing higher alcohol strains.

关键词： Saccharomyces cerevisiae Higher alcohol transcriptomics sequencing Alcoholic beverage α-keto acid decarboxylase PDC1 gene

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