检索结果-南通市图书馆

中国植物学会第十六次全国会员代表大会暨85周年学术年会

作者： Boyong Liao Kaikai Meng Qianyi Yin Cuiying Huang Hanzhang Song Qiang Fan Wenbo Liao Sufang Chen State Key Laboratory of Bio-control Guangdong Provincial Key Laboratory of Plant ResourceAnd School of life scienceSun Yat-sen University

Morinda L.（Rubiaceae） contains approximately 80-100 species,many of which are famous local medical plants.However,the survival of the natural populations of these species are always threatened by excessive collections,calling for widely scientific attentions.M.officinalis is,known as one the four medicine plants in South China,becomes extinct in the natural environments.In this study,we present the first transcriptome analysis of M.officinalis using the Illumina sequencing technology.More than 11 million raw reads were generated,and de novo assembled into 51,049 unigenes,with an average length of 801 bp.The similarity search indicated that 25,547 unigenes（50.04 %） had significant similarity with proteins in the NCBI Non-redundant（NR） database,25,546 and 18646 unigenes were assigned with Gene Ontologies（GO） and Eukaryotic Orthologous Groups（KOG）,respectively,and a total of 9,886 unigenes were mapped into 241 pathways in the Kyoto Encyclopedia of Genes and Genomes Pathway database（KEGG）.Some abundant transcripts related to Genetic information,including Folding,sorting and degradation（843,8.53%） and Translation（770,7.79%） were identified in the M.officinalis transcriptome.In total,8,064 potential EST-SSRs were identified among the unigenes.The most abundant type of repeat was mononucleotide（4221,52.34 %）,followed by trinucleotide（1588,19.69 %） and dinucleotide（1040,12.90 %） repeats in perfect EST-SSRs.Finally,5605 SSR primer pairs were designed successful.In addition,a total of 100 potential marker sites were selected to validate the assembly quality,and 24 pairs of polymorphic primers were characterized using four populations of M.officinalis from Guangdong province.This study generated a substantial fraction of M.officinalis transcriptome sequences,which were very useful resources for gene discovery and molecular marker development.

关键词： Morinda officinalis transcriptome sequence EST-SSR markers Cross species

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Enhanced cobalamin biosynthesis in Ensifer adhaerens by regulation of key genes with gradient promoters

引用

Synthetic and Systems Biotechnology 2022年第3期7卷 941-948页

作者： Sha Xu Zhiqiang Xiao Shiqin Yu Weizhu Zeng Yongming Zhu Jingwen Zhou National Engineering Laboratory for Cereal Fermentation Technology Jiangnan University1800 Lihu RoadWuxiJiangsu214122China School of Biotechnology and Key Laboratory of Industrial Biotechnology Ministry of EducationJiangnan University1800 Lihu RoadWuxiJiangsu214122China Science Center for Future Foods Jiangnan University1800 Lihu RoadWuxiJiangsu214122China Jiangsu Provisional Research Center for Bioactive Product Processing Technology Jiangnan University1800 Lihu RoadWuxiJiangsu214122China Hunan Hongying Biotechnology Co.Ltd. 10 Hongying RoadJinshiHunan415400China

Cobalamin is an essential human vitamin widely used in the pharmaceutical,food,and feed additive industries and currently produced by bacteria or archaea.Ensifer adhaerens HY-1 is an industrial strain that also produces cobalamin.However production outputs are poor and the specific synthesis pathways require characterization.In this study,the whole genome sequence of E.adhaerens HY-1 was generated and annotated,and genes associated with cobalamin biosynthesis were identified.Then,three genes,CobSV,CobQ,and CobW were identified as the most efficient ones for enhancing cobalamin synthesis.By transcriptome sequencing of E.adhaerens HY-1 cells at different growth stages,65 endogenous promoters with different gradient strengths were identified.After combined expression of different strength promoters and key genes,a high cobalamin-producing recombinant strain,‘hmm’(genotype:PmetH-CobSV-PibpA-CobQ-Pmdh-CobW),was generated.Cobalamin production was 143.8 mg/L in shaking flasks,which was 41.0%higher than the original strain.Cobalamin production was further enhanced to 171.2 mg/L using fed-batch fermentation.Importantly,our data and novel approach provide important references for the analysis of cobalamin synthesis and other metabolites in complex metabolic pathways.

关键词： Ensifer adhaerens Cobalamin Genome sequence transcriptome sequence Promoter Overexpression Combinations

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：