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中国植物病理学会第十一届全国会员代表大会暨2018年学术年会

作者： Cong Jie Liu Jinliang Zhang Yanhua Zhang Xianghui Pan Hongyu College of Plant Sciences Jilin University

Sclerotinia sclerotiorum(Lib.) de Bary is a necrotrophic plant pathogenetic fungus with broad host range and worldwide *** sclerotia and cushion of *** will be induced and produced into pigmented multicellular structures from the aggregation of hyphae during the vegetative development stages respectively under ambient pH,cAMP and light *** are requried for the production of fruiting *** the cushion play a central role in the life and infection cycles of this *** the basis of some research previously,Ss-Fkh1,forkhead(FKH)-box-containing protein,is an important transcriptional factor involved information and development of sclerotia and *** investigate the role of Ss-Fkh\ in ***,the partial sequence of Ss-Fkh1 was cloned and RNA interference(RNAi)-based gene silencing was employed to alter the expression of *** mutants with significantly reduced Ss-Fkhl RNA levels exhibited slow-growing hypha and developmental defects *** addition,the expression levels of a set of putative melanin biosynthesis-related laccase genes and a polyketidesynthase-encoding gene were significantly down-regulated insilenced *** test results demonstrated that RNAi-silenced strains was significantly compromised with the development of a smaller infection lesion on tomato ***,the resulting mutant displays series phenotypes that mycelial growth rate decreased significantly when compared Ss-Fkh1 knockout mutant with the wild-type,it’s development stage remained in S1 stage of ***,the results suggest that Ss-Fkh1 is involved in hyphalgrowth,virulence and sclerotial formation in ***.

关键词： Sclerotinia sclerotiorum transcriptional factor SsFKH1 Sclerotia Cushion

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transcriptional factors related to flavonoid biosynthesis in the medicinal plant epimedium sagittatum(Berberidaceae)

Transcriptional factors related to flavonoid biosynthesis in...

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从植物科学到农业发展——2012全国植物生物学大会

作者： Ying Wang Wuhan Botanical Garden,Chinese Academy of Sciences,Wuhan,Hubei,China 430074.

Herba epimedi,prepared from Epimedium species,is known as Horny Goat Weed and used as traditional Chinese *** is known about the molecular mechanism for the biosynthesis of bioactive compounds in Epimedium species,including icariin,epimedin A, epimedin B,and epimedin *** EST dataset was generated by 454 GX-FLS sequencing using red-magenta fully expanded leaves and flowers of *** Maxim. Consequently,511,102,and 383 consensus sequences were annotated and involved in flavonoid,carotenoid,and terpenoid pathways,***,these gene fragments and other flavonoid gene fragments isolated by SSH and homolog cloning were used as core fragments for cloning full-length genes involving flavonoid biosynthesis. Subsequently,the structural genes involved in flavonoid pathway were obtained and several full-length transcriptional factors,such as MYB,bHLH,and WD,were also isolated from Epimedium *** analysis of these genes discovered a set of genes with positive and negative correlation with the accumulation of bioactive ***, two MYB transcriptional factors have been functionally characterized for the biosynthesis of icarrin and *** bioinformatic analysis and characterization of genes involved in the flavonoid biosyntheses pathway will facilitate the study toward uncovering the molecular mechanism of flavonoid accumulation in Epimedium species.

关键词： Epimedium sagittatum flavonoid biosynthesis transcriptional factor structure gene

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transcriptional Crosstalk between Nuclear Receptors and Cytokine Signal Transduction Pathways in Immunity

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Cellular & Molecular Immunology 2004年第6期1卷 416-424页

作者： LihuaWang XiaohuZhang WilliamL.Farrar BasicResearchProgram SAIC-FrederickNationalCancerInstitute-FrederickFrederickUSA. CytokineMolecularMechanismsSection LaboratoryofMolecularImmunoregulationDivisionofBasicSciencesNationalCancerInstitute-FrederickFrederickUSA.

The nuclear receptor superfamily and the transcriptional factors associated with cytokines are inherently different families of signaling molecules and activate gene transcription by binding to their respective responsive ***,it has become increasingly clear from our works and others that nuclear receptors are important regulators of cytokine production and function through complex and varied interactions between these distinct transcriptional *** review provides a general overview of the mechanism of action of nuclear receptors and their transcriptional crosstalk with transcriptional factors associated with cytokine transduction *** of the most important mechanistic aspects is protein to protein interaction through a direct or co-regulator-mediated indirect *** crosstalk is crucially involved in physiological and therapeutic roles of nuclear receptors and their iigands in immunity, inflammation and cytokine-related *** & Molecular Immunology.2004;1(6):416-424.

关键词： nuclear receptor cytokine signaling transduction crosstalk transcriptional factor immunity

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维普期刊数据库

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The PhoR/PhoP two-component system regulates fengycin production in Bacillus subtilis NCD-2 under low-phosphate conditions

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Journal of Integrative Agriculture 2018年第1期17卷 149-157页

作者： GUO Qing-gang DONG Li-hong WANG Pei-pei LI She-zeng ZHAO Wei-song LU Xiu-yun ZHANG Xiao-yun MA Ping Institute of Plant Protection Hebei Academy of Agriculture and Forestry Sciences/Integrated Pest Management Center of Hebei Province/Key Laboratory of IPM on Crops in Northern Region of North ChinaMinistry of Agriculture

Bacillus subtilis strain NCD-2 is an excellent biocontrol agent for plant soil-borne diseases, and the lipopeptide fengycin is one of the active antifungal compounds in strain NCD-2. The regulator PhoP and its sensor kinase PhoR compose a two-component system in B. subtilis. In this study, the phoR- and phoP-knockout mutants were constructed by in-frame deletion and the role of PhoR/PhoP on the production of fengycin was determined. Inactivation of phoR or phoP in B. subtilis decreased its inhibition ability against Botrytis cinerea growth in vitro compared to the strain NCD-2 wild type. The lipopeptides were extracted from strain NCD-2 wild type and its mutant strains by hydrochloric acid precipitate, and the lipopeptides from phoR-null mutant orphoP-null mutant almost lost the inhibition ability against B. cinerea growth compared to the lipopeptides from strain NCD-2 wild type. Fast protein liquid chromatography （FPLC） analysis of the lipopeptides showed that inactivation of phoR or phoP genes reduced the production of fengycin by strain NCD-2. The fengycin production abilities were compared for bacteria under low-phosphate medium （LPM） and high-phosphate medium （HPM）, respectively. Results indicated that the regulation of fengycin production by the PhoR/PhoP two-component system occurred in LPM but not in HPM. Reverse transcriptionaI-PCR confirmed that the fengycin synthetase gene fenC was positively regulated by phoP when cultured in LPM. All of these characteristics could be partially restored by complementation of intact phoR or phoP gene in the mutant. These data indicated that the PhoR/PhoP two-component system greatly regulated fengycin production and antifungal ability in B. subtilis NCD-2 mainly under low-phosphate conditions.

关键词： Bacillus subtilis two-component system Iipopeptides transcriptional factor PhoR/PhoP

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维普期刊数据库

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A transferred regulator that contributes to Xanthomonas oryzae *** oxidative stress adaptation and virulence by regulating the expression of cytochrome bd oxidase genes

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Journal of Integrative Agriculture 2022年第6期21卷 1673-1682页

作者： WANG Pei-hong WANG Sai NIE Wen-han WU Yan Iftikhar AHMAD Ayizekeranmu YIMING HUANG Jin CHEN Gong-you ZHU Bo Shanghai Yangtze River Delta Eco-Environmental Change and Management Observation and Research Station/Shanghai Cooperative Innovation Center of Agri-Seeds School of Agriculture and BiologyShanghai Jiao Tong UniversityShanghai 200240P.R.China Department of Environmental Sciences COMSATS University IslamabadVehari-CampusVehari 61100Pakistan

Horizontal gene transfer(HGT)has been well documented as a driving force in the evolution of *** has been shown that a horizontally acquired gene,xoc_2868,involved in the global response against oxidative stress and pathogenicity of Xanthomonas oryzae *** strain ***,as a transcriptional factor(TF),the regulatory mechanism of XOC_2868 has not yet been ***,evolutionary analysis suggested XOC_2868 might be co-transferred with its physically proximate downstream genes from a Burkholderiaceae ***,RNA-seq data of wild-type(BLS256)andΔxoc_2868 strains under oxidative stress showed that XOC_2868 did not regulate the expression of its adjacent genes,but remarkably influenced the expression of several genes involved in the extracellular polysaccharide(EPS)production and xanthan *** immunoprecipitation-sequence(ChIP-seq)combined with transcriptome analysis revealed that XOC_2868 directly regulates a cydAB operon,encoding two subunits of cytochrome bd oxidase and involved in redox *** withΔxoc_2868 strain,cydA-and cydAB-knockout mutants also showed a higher sensitivity to H_(2)O_(2)along with a reduced bacterial virulence compared with the wild-type ***,our findings raise the possibility of regulatory circuit evolution shaped by HGT and driven by selection and reveal a novel regulatory pathway that regulates the expression of cytochrome bd oxidase and thus contributes to the virulence of BLS256.

关键词： HGT transcriptional factor Xanthomonas oryzae pv.oryzicola oxidative stress adaptation and virulence

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维普期刊数据库

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A single transcription factor promotes both yield and immunity in rice

A single transcription factor promotes both yield and immuni...

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2018全国植物生物学大会

作者： Jing Wang Lian Zhou Hui Shi Mawsheng Chern Hong Yu Hong Yi Min He Junjie Yin Xiaobo Zhu Yan Li Weitao Li Jiali Liu Jichun Wang Xiaoqiong Chen Hai Qing Yuping Wang Guifu Liu Wenming Wang Ping Li Xianjun Wu Lihuang Zhu Jian-Min Zhou Pamela C.Ronald Shigui Li Jiayang Li Xuewei Chen State Key Laboratory of Crop Genetics of Disease Resistance and Disease control State Key Laboratory of Hybrid RiceKey Laboratory of Major Crop Diseases and Collaborative Innovation Center for Hybrid Rice in Yangtze River BasinRice Research InstituteSichuan Agricultural University at Wenjiang Department of Plant Pathology and the Genome Center University of California State Key Laboratory of Plant Genomics and National Center for Plant Gene Research Beijing Institute of Genetics and Developmental BiologyChinese Academy of Sciences University of the Chinese Academy of Sciences

Plant immunity often penalizes growth and *** transcription factor Ideal Plant Architecture 1(IPA1) reducesunproductivetillers and increases grains per panicle,which results in improved rice *** we report that higher IPA1 levels enhance ***,phosphorylation of IPAl at amino acid S163 within its DNA-binding domain occurs in response to infection by the fungus Magnaporthe oryzae,and alters the DNA binding specificity of *** IPAl binds to the promoter of the pathogen defense gene WRKY45 and activates its expression,leading to enhanced disease ***1 returns to a non-phosphorylated state within 48 hours after infection,resuming support of the growth needed for high *** IPA1 promotes both yield and disease resistance by sustaining a balancebetween growth and immunity.

关键词： transcriptional factor IPA1 Yield Immunity Rice

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Genome-wide analysis of the AP2/ERF gene family in maize

Genome-wide analysis of the AP2/ERF gene family in maize

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2012年全国玉米遗传育种学术研讨会暨新品种展示观摩会

作者： Min Huang~(1,2),Shuiyuan Cheng~2,Zuxin Zhang~3,Hewei Du~1 (1.College of life science,Yangtze University,Jingzhou,Hubei 434025,P.R.China 2.Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization,Huanggang Normal Universtiy,Huanggang,Hubei 438000,P.R.China 3.College of plant science and technology,Huazhong Agricultural University,Wuhan 430070,P.R.China)

Plants experience various abiotic stresses during their life cycle,such as drought,salinity,and extreme temperatures, which cause extensive losses to agricultural production *** stress has become one of major limiting factor of crop loss, reducing extensive yields for most major crop plants by more than 50% .Plant adaptation to abiotic stresses is controlled by cascades of molecular *** relatively little is known about these molecular networks of plant tolerance to abiotic stress,a large number of stress - associated genes and signal - transduction systems have been *** researches show that AP2/ERF(APETALA2 /ethylene response factors) subfamily transcription factors play important roles in directing changes of gene expression during abiotic stress. AP2/ERF genes form a large multigene family,containing either one or two AP2 domains,and are classified into five subfamilies: AP2,DREB(dehydration responsive element binding),RAV(related to ABI3/VP),ERF,and others(Sakuma et al.,2002).ERF and DREB subfamily can be further divided into 6 subgroups respectively,ERF with B1-6 6 subgroups,and DREB subfamily with A1-6 6 *** has been demonstrated that AP2/ERF proteins play an important role in regulation of plant growth and development, response to environmental ***2/ERF genes play a variety of roles throughout the plant life cycles,however,few reports of AP2/ERF family are available for maize. In the present report,330 maize AP2/ERF genes were downloaded from PlnTFDB database,and 84 genes were belongs to 35 locus, ***,281 AP2/ERF genes were found from PlnTFDB database.121 maize AP2/ERF genes were obtained from NCBI and Maizesequence database through BLASTP,while 69 of 121 genes have founded in *** 52 of 120 genes were chosen. Above all,333 maize AP2/ERF genes were *** order to exclude false - positive,the amino acid sequence of 330 AP2/ ERF genes was used as a query to search in maiz

关键词： Maize(Zea mays L.) AP2/ERF transcriptional factor Bioinfomatics In silico cloning

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A Homeodomain Protein Required for Salt Tolerance in Arabidopsis thaliana

A Homeodomain Protein Required for Salt Tolerance in Arabido...

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中国遗传学会植物遗传和基因组学专业委员会2009年学术研讨会

作者： Rong Cui,Ping Zhao,and Chengbin Xiang~* School of Life Sciences,University of Science and Technology of China,Hefei 230027

Homeodomain proteins usually play important roles in plant ***,a number of such proteins have been found involved in stress adaptation,possibly functioning as developmental regulators specifically involved in regulati... 详细信息

关键词： salt tolerance homeodomain protein transcriptional factor Arabidopsis thaliana

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