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Transcriptomic and physiological analyses identifying Lanzhou lily(Lilium davidii ***)drought adaptation strategies

Horticultural Plant Journal 2023年第1期9卷 145-157页

作者： Wenmei Li Yajun Wang Heng Ren Zhihong Guo Na Li Chengzheng Zhao Zhongkui Xie Northwest Institute of Eco-environment and Resources Chinese Academy of SciencesLanzhouGansu 730000China University of Chinese Academy of Sciences Beijing 100049China

Drought stress is the main limiting plant growth factor in arid and semiarid *** Lanzhou lily(Lilium davidii ***)is the only sweet-tasting lily grown in these regions of China that offers highly edible,medicinal,health,and ornamental *** Tresor lily is an ornamental flower known for its strong *** were grown under three different drought intensity treatments,namely,being watered at intervals of 5,15,and 25 d(either throughout the study or during specific growth stages).We measured the biomass,leaf area,photosynthetic response,chlorophyll content(SPAD value),and osmoregulation of both the Lanzhou lily and the Tresor lily(Lilium‘Tresor’).Additionally,we employed RNA sequencing(RNA-Seq)and qRT-PCR to investigate transcriptomic changes of the Lanzhou lily in response to drought *** showed that under drought stress,the decreasing rate in the Lanzhou lily bulb weight was lower than the corresponding Tresor lily bulb rate;the net photosynthetic rate,transpiration rate,and stomatal conductance of the Lanzhou lily were all higher compared to the Tresor lily;osmoregulation constituents,such as glucose,fructose,sucrose,trehalose,and soluble sugar,in the Lanzhou lily were comparatively higher;PYL,NCED,and ERS genes were significantly expressed in the Lanzhou *** moderate drought,the biosynthesis of flavonoids,circadian rhythms,and the tryptophan metabolism pathway of the Lanzhou lily were all *** severe drought stress,fatty acid elongation,photosynthetic antenna protein,plant hormone signal transduction,flavone and flavonol biosynthesis,and the carotenoid biosynthesis pathway were all *** Lanzhou lily adapted to drought stress by coordinating its organs and the unique role of its bulb,regulating photosynthesis,increasing osmolyte content,activating circadian rhythms,signal transduction,fatty acid elongation metabolism,and phenylalanine and flavonoid metabolic pathways,which may collectively be the

关键词： Drought stress Adaptation strategy Osmolytes Lanzhou lily Physiological characterization transcription profiles

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DNA microarray technology and its application in fish biology and aquaculture

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Frontiers in Biology 2009年第3期4卷 305-313页

作者： Jianshe ZHANG Wuying CHU Guihong FU Department of Bioengineering and Environmental Science Changsha UniversityChangsha 410003China

Fishery is an important industry in China as well as in the rest of the world,and it provides a human food resource containing high-quality *** practice in aquaculture requires a full understanding of the genomic controls and transcriptional profiles of cultured fish *** in aquaculture can be made by regulation of the expression of functional *** technology is a powerful tool for rapid screening of genes or transcriptional profiles in a particular fish or for a particular economic character;for example,genes that are related to growth and disease control in the *** review provides a brief introduction to microarray technology and its methods and applications,together with a discussion of the achievements in fish biology that have resulted from this technology.

关键词： complementary DNA(cDNA)microarray bioinformatics transcription profiles teleost fish aquaculture

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