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Retrograde tracer systems based on Pseudorabies virus

Retrograde tracer systems based on Pseudorabies virus

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中国神经科学学会第十二届全国学术会议

作者： Jia Fan Miu Huan Xu Xiaoqin Lv Pei Xu Fuqiang Brain research center Wuhan Institute of Physics and Mathematics Chinese Academy of Sciences University of Chinese Academy of Sciences Center for Excellence in Brain Science and Intelligence Technology Chinese Academy of Sciences

Objective Neural circuit is the foundation of the brain function. Tracers based on neurotropic viruses, such as pseudorabies virus, rabies virus, vesicular stomatitis virus, herpes simplex virus, have been developed for displaying the full morphology of a neuron and mapping the neural circuit. In these systems, the pseudorabies virus is a DNA virus, it can infect swine, mouse, rat and other animals. However, it cannot infect human. The tracer based on the Bartha strain, when it enters a neuron, it can transport in the neuron and spread from a neuron to the connected neuron with retrograde pattern. The tool could be used to determine the input circuit. The virus can be modified to develop various retrograde tools to resolve different questions. Methods Two tracer systems based on pseudorabies virus could be developed, i) transmonosynaptic tracer（specific cell type and non-specific cell type）: the TK gene is a switch, which could be deleted to prepare TK-deleted pseudorabies virus. When the helper virus expressing TK gene was injected into the target region, the TK was expressed and it can induce the replication of TK-deleted pseudorabies virus in neuron and assemble new viruses, then these new viruses can trans-monosynapse. ii) transmultisynaptic tracer（specific cell type and non-specific cell type）: the cre and flp sitespecific recombinases are excellent tools, and many transgenic animal models have been developed, such as PV-Cre, Thy1-Cre. Based on these advantages, the pseudorabies virus could be modified to determine the cell type specific neural circuit. Results and Conclusion The recombinant viruses and the wild type viruses are similar in viral growth, and they can label neuron and depict the neural circuit with retrograde pattern.

关键词： Retrograde Pseudorabies virus trans-monosynapse trans-multisynapse

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