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Agricultural Science & Technology 2017年第2期18卷 277-279页

作者：高桂生安继伟胡铁锋高光平史秋梅张艳英邵新华梁银聚河北省预防兽医学重点实验室河北科技师范学院动物科技学院河北昌黎066600 乐亭县畜牧兽医局河北乐亭063600 河北新华科极兽药有限公司河北栾城051430

According to the theory of chinese veterinary medicine and characteristics of chinese herbal medicine, four different preparations of compound chinese medicine ＂Zengrujianniusan＂ were composed, and the prepared water decoction was used to carry on the bacteriostatic test on main pathogens of cow recessiveness mastitis. The results showed that the four different prescriptions of water decoction all had antibacterial effects. The prescription 3 was sensitive to Staphylococcus aureus and Streptococcus agalactiae, while the other three prescriptions showed high sensitivity, and the prescription 3 had the strongest bacteriostatic effects.

关键词： traditional chinese medicine preparation Cow recessiveness mastitis Pathogenic bacteria Bacteriostatic effect

Determination of strychnine and brucine in traditional chinese medicine preparations by capillary zone electrophoresis with micelle to solvent stacking

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chinese Chemical Letters 2011年第3期22卷 330-333页

作者： Xiao Fang Yin Zhi Li Shuai Hua Zhang Chun Xia Wu Chun Wang Zhi Wang College of Science Agricultural University of Hebei Baoding 071001 China

A novel micelle to solvent stacking on-line sample preconcentration technique in capillary zone electrophoresis（MSS-CZE） has been developed to determine the strychnine and brucine in traditional chinese medicine *** optimal running buffer was 30 mmol/L H_3PO_4 containing 20%acetonitrile at pH *** sample matrix was 8 mmol/L H_3PO_4 containing 5 mmol/L sodium dodecyl sulfate（SDS） at pH *** established MSS-CZE method afforded more than 50-fold improvements in concentration sensitivity compared with typical CZE-UV *** calibration curve was linear in the range from 0.2 to 15.0μg/ mL for both strychnine and brucine,with correlation coefficients of 0.9984 and 0.9976,*** limits of detection（5/ N = 3：1） for strychnine and brucine were 0.02 and 0.05μg/mL,*** MSS-CZE method has been successfully applied to the analysis of strychnine and brucine in chinese medicinal preparations.

关键词： On-line sample preconcentration Micelle to solvent stacking Capillary zone electrophoresis Strychnine Brucine traditional chinese medicine preparation

Application and Practice of traditional chinese medicine Characteristic Technology Inheritance in Research and Development of traditional chinese medicine preparations in Medical Institutions

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Agricultural Biotechnology 2023年第5期12卷 88-90,107页

作者： Xiu CHENG Hai ZHAO Shujie KOU Mingqin CHEN Liang YANG Changshun People's Hospital Guizhou ProvinceChangshun 550700China The Second Affiliated Hospital of Guizhou University of traditional chinese medicine Guiyang 550002China

Medical institution preparations play an important role in the development of hospitals,being one of the main forms of traditional chinese medicine clinical characteristic medication,as well as an important carrier for the inheritance and innovation of traditional chinese *** recent years,the research and development of traditional chinese medicine preparations has become a hot spot in the research and development of preparations by medical *** chinese medicine preparations prepared by traditional processes and their entrusted preparation matters have changed from the current licensing management to filing *** registration model of traditional chinese medicine preparations has undergone significant changes,emphasizing more on real data and evaluation results from the objective world,respecting traditional medication experience,and emphasizing the inheritance and innovative development of traditional technological *** the process of following teachers in the inheritance project of traditional chinese medicine,we combined the inheritance of traditional chinese medicine characteristic technology with the research and development practice of traditional chinese medicine preparations in medical institutions,and focused on the analysis and discussion of technical problems such as prescription screening,dosage form selection,process route design and clinical observation of traditional chinese medicine preparations,hoping to provide reference for scholars in the same field of traditional chinese medicine.

关键词： Inheritance of traditional chinese medicine characteristic technology traditional chinese medicine preparation Medical institutions Practical analysis

Evaluation of Antitumor,Immunomodulatory and Free Radical Scavenging Effects of A New Herbal Prescription Seaweed Complex preparation

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Journal of Ocean University of China 2013年第3期12卷 515-520页

作者： LIU Xin SHAO Changlun KONG Wenwen FANG Yuchun WANG Changyun Key Laboratory of Marine Drugs Ministry of EducationMarine Drug and Food InstituteOcean University of China Biology Institute of Shandong Academy of Sciences/Key Laboratory for Biosensors of Shandong Province

Seaweed Complex preparation （SCP） is a clinical traditional chinese medicine preparation which is composed of seven traditional chinese herbs, and it has been used for treatment of lung cancer, liver cancer and digestive cancer. However, little infor- mation is available about the pharmacodynamic basis. The antitumor, immunomodulatory and free radical scavenging effects of SCP were evaluated in this study. Transplanted tumor in vivo method was used to determine the antitumor effect. The effects on spleno- cyte proliferation and phagocytosis of macrophages in tumor-bearing mice were measured by the MTT method and the phagocytizing cock red blood cell （CRBC） method respectively. The scavenging activities of SCP on DPPH and hydroxyl radicals in vitro were investigated. It was found that the medium-dose and high-dose of SCP could significantly inhibit the growth of transplanted hepatic tumor of murine hepatocarcinoma cell line H22, and promote proliferation of splenocytes and phagocytosis of macrophages. SCP possessed noticeable scavenging activities on DPPH and hydroxyl radicals. The antiturnor effects of SCP might be achieved by im- proving immune system and scavenging free radicals, which is in accordance with the viewpoint of traditional chinese medicine in promoting the body resistance and eliminating pathogenic factors for cancer treatment.

关键词： traditional chinese medicine preparation Seaweed complex preparation antitumor immunomodulatory free radicalscavenging

Screening and Efficacy Observation of traditional chinese medicine Against Chicken Pathogenic Escherichia coli

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Agricultural Science & Technology 2009年第1期10卷 140-144页

作者：庞向红褚秀玲苏建青梁淑珍李全福胡满河北农业大学动物科技学院河北保定071001 河北北方学院动物科技学院河北张家口075131 聊城大学农学院山东聊城252059

[ Objective] The aim of this study was to lay a foundation for obtaining effective traditional chinese medicine preparations controlling chicken colibacillosis. [ Method] With the western medicine Lomeflbxacin Hydrochloride and Sodium Chloride Injection as the control, drug sensitivity tests of 15 single traditional chinese herbs and seven self-made chinese herbal compounds were conducted. Subsequently, the single traditional chinese herbs presenting good in vitro antibacterial effects against Escherichia coli and all the seven compounds were given to the chicks artificially infected with E. coil to observe their efficacies. [ Result] The results of drug sensitivity tests showed that all the 15 traditional chinese herbs had certain antibacterial effect against E. coli, among which aloe, pomegranate rind and radix sanguisorbae were the best. Compound 1, 2 and 5 had good antibacterial effects in vitro. By in vivo test, traditional chinese medicines showed superior efficacies on infected chicks than western medicine, and compound 1 and 2 performed well. [ConclusiOn] traditional chinese medicine preparations are good at controlling Chicken colibacillosis, but their bacteriostasis mechanism in vivo was different with that in vitro.

关键词： traditional chinese medicine preparation Drug sensitivity test Chicken colibacillosis In vivo test