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A new anterograde trans-synaptic Tracer based on Sindbis virus

Neural Regeneration Research 2022年第12期17卷 2761-2764页

作者： Xiang-Wei Shi Fan Jia Pei Lyu Fu-Qiang Xu State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics Key Laboratory of Magnetic Resonance in Biological SystemsWuhan Center for Magnetic ResonanceInnovation Academy for Precision Measurement Science and TechnologyChinese Academy of SciencesWuhanHubei ProvinceChina Guangdong Provincial Key Laboratory of Brain Connectome and Behavior CAS Key Laboratory of Brain Connectome and Manipulationthe Brain Cognition and Brain Disease Institute(BCBDI)Translational Research Center for the Nervous System(TRCNS)Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research InstitutionsShenzhenGuangdong ProvinceChina NMPA Key Laboratory for Research and Evaluation of Viral Vector Technology in Cell and Gene Therapy Medicinal Products Key Laboratory of Quality Control Technology for Virus-Based TherapeuticsGuangdong Provincial Medical Products AdministrationShenzhen Key Laboratory of Viral Vectors for BiomedicineShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong ProvinceChina University of Chinese Academy of Sciences BeijingChina Wuhan National Laboratory for Optoelectronics Huazhong University of Science and TechnologyWuhanHubei ProvinceChina

Mapping neural circuits is critical for understanding the structure and function of the nervous system.Engineered viruses are a valuable tool for tracing neural circuits.However,current Tracers do not fully meet the needs for this approach because of various drawbacks,such as toxicity and characteristics that are difficult to modify.Therefore,there is an urgent need to develop a new Tracer with low toxicity and that allows for long-term studies.In this study,we constructed an engineered Sindbis virus(SINV)expressing enhanced green fluorescent protein(EGFP)reporter gene(SINV-EGFP)and found that it had no significant difference in biological characterization compared with the wild-type Sindbis virus in BHK-21 cells and neurons in vitro.We injected the virus into the visual circuit of mouse brain and found that the virus infected neurons in the local injected site and anterogradely spread in the neural circuits.Although the efficiency of transmission was limited,the findings demonstrate that SINV can be used as a new anterograde Tracer to map neural circuits in mouse brain and that it spreads exclusively in the anterograde direction.Further,use of SINV in mouse brain research will provide longer time windows for circuit tracing than is possible with herpes simplex virus and vesicular stomatitis virus Tracers.

关键词： anterograde lateral geniculate nucleus mouse brains neural circuit neurons retina Sindbis virus superior colliculus synapse Tracer

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Comparison of smoothness-constrained and geostatistically based cross-borehole electrical resistivity tomography for characterization of solute Tracer plumes

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Water Science and Engineering 2016年第4期9卷 274-286页

作者： Andreas Englert Andreas Kemna Jun-feng Zhu Jan Vanderborght Harry Vereecken Tian-Chyi J.Yeh Institute of Geology Mineralogy and GeophysicsRuhr University Bochum Department of Geophysics Steinmann InstituteUniversity of Bonn Kentucky Geological Survey University of Kentucky Agrosphere Institute for Bio- and Geosciences(IBG-3)Forschungszentrum Jlich GmbH Department of Resources Engineering National Cheng Kung University Department of Hydrology and Water Resources The University of Arizona

Experiments using electrical resistivity tomography(ERT) have shown promising results in reducing the uncertainty of solute plume characteristics related to estimates based on the analysis of local point measurements only.To explore the similarities and differences between two cross-borehole ERT inversion approaches for characterizing salt Tracer plumes,namely the classical smoothness-constrained inversion and a geostatistically based approach,we performed two-dimensional synthetic experiments.Simplifying assumptions about the solute transport model and the electrical forward and inverse model allowed us to study the sensitivity of the ERT inversion approaches towards a variety of basic conditions,including the number of boreholes,measurement schemes,contrast between the plume and background electrical conductivity,use of a priori knowledge,and point conditioning.The results show that geostatistically based and smoothness-constrained inversions of electrical resistance data yield plume characteristics of similar quality,which can be further improved when point measurements are incorporated and advantageous measurement schemes are chosen.As expected,an increased number of boreholes included in the ERT measurement layout can highly improve the quality of inferred plume characteristics,while in this case the benefits of point conditioning and advantageous measurement schemes diminish.Both ERT inversion approaches are similarly sensitive to the noise level of the data and the contrast between the solute plume and background electrical conductivity,and robust with regard to biased input parameters,such as mean concentration,variance,and correlation length of the plume.Although sophisticated inversion schemes have recently become available,in which flow and transport as well as electrical forward models are coupled,these schemes effectively rely on a relatively simple geometrical parameterization of the hydrogeological model.Therefore,we believe that standard uncoupled ERT inverse approaches,like the ones discussed and

关键词： Electrical resistivity tomography Inversion technique Solute Tracer plume Synthetic experiment Plume characteristics

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Performance evaluation of a water erosion Tracer using plot-scale experiments and process-based modeling

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International Soil and Water Conservation Research 2023年第4期11卷 622-632页

作者： João M.Villela Jamil A.A.Anache Alex M.Watanabe Dennis C.Flanagan Edson C.Wendland Silvio Crestana EMBRAPA-Brazilian Agricultural Research Corporation LNNA-National Laboratory of Nanotechnology for AgrobusinessSt.XV de NovembroSão CarlosSP13560-970Brazil University of São Paulo(USP) São Carlos School of Engineering.Graduate Program in Environmental Engineering Sciences(PPG-SEA)Av.Trabalhador São-CarlenseCxP.359São CarlosSP13566590Brazil Department of Hydraulics and Sanitation São Carlos School of Engineering(EESC)University of São Paulo(USP)CxP.359São CarlosSP13566-590Brazil Federal University of Mato Grosso Do Sul CxP.549Campo Grande.MS.79070-900Brazil USDA-Agricultural Research Service National Soil Erosion Research Laboratory275 S.Russell StWest LafayetteIN47907USA

Socioeconomic and environmental losses caused by water erosion have highlighted the importance of quantifying and understanding the dynamics of soil redistribution in the landscape to develop effective soil management practices.Several methods are applied to estimate erosion/deposition rates and identify sources of sediments,among them,the one that uses rare earth elements(REE)as a Tracer stands out.However,an alternative not yet explored that can benefit the accuracy of the estimates provided by the method is using a Tracer containing a chemical signature composed of more than one REE.The present study aimed to evaluate the performance of a new water erosion Tracer based on montmoril-lonite labeled with rare earth elements(La40-MMT).The innovative aspects of this La40-MMT Tracer include its highly stable multi-chemical signature(Nd^(3+),La^(3+),and Pr^(3+)),which enhances Tracer detection in the environment,and its low production cost due to the use of an industrial residue in the synthesis process.The Tracer was evaluated for a typical soil of the Cerrado biome,using a natural rainfall field-scale plot-NRFP(5 m × 20 m)and a physical predictive erosion model(WEPP).The results showed that the La40-MMT Tracer could be used to estimate erosion/deposition rates,with agreement between the values observed with the Tracer and the WEPP model.Thus,this study confirmed the great potential of La40-MMT as a tool to identify patterns of soil redistribution at the field scale and aid in the validation of erosion models.

关键词： Soil erosion Tracer Rare earth elements Deposition Sediment source WEPP Model

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Self-adaptive behavior of nunchakus-like Tracer induced by active Brownian particles

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Chinese Physics B 2022年第4期31卷 90-95页

作者：夏益祺冯国强谌庄琳 Department of Physics and Electronic Engineering Yancheng Teachers UniversityYancheng 224007China Center for Soft Condensed Matter Physics&Interdisciplinary Research School of Physical Science and TechnologySoochow UniversitySuzhou 215006China Department of Chemistry and Environmental Engineering Shenzhen UniversityShenzhen 518060China

We design a nunchakus-like Tracer and investigate its self-adaptive behavior in an active Brownian particle(ABP)bath via systematically tuning the self-propelled capability and density of ABPs.Specifically,the nunchakus-like Tracer will have a stable wedge-like shape in the ABP bath when the self-propelled force is high enough.We analyze the angle between the two arms of the Tracer and the velocity of the joint point of the Tracer.The angle exhibits a non-monotonic phenomenon as a function of active force.However,it increases with density of ABPs increasing monotonically.A simple linear relationship between the velocity and the self-propelled force is found under the highly active force.In other words,the joint points of the Tracer diffuse and the super-diffusive behavior can make the relation between the self-propelled force and the density of ABPs persist longer.In addition,we find that the Tracer can flip at high density of ABPs.Our results also suggest the new self-adaptive model research of the transport properties in a non-equilibrium medium.

关键词： Tracer self-adaptive super-diffusion active Brownian particles

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Cisco Packet Tracer支持下的创客教育——以“网络系统设计与集成”课程为例

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电化教育研究 2018年第5期39卷 74-78页

作者：王云付蓉山西师范大学山西基础教育质量提升协同创新中心山西临汾041000 山西师范大学教育科学学院山西临汾041000

近年来,创客教育发展迅速,影响突出,符合时代发展需求。文章以"网络系统设计与集成"课程为例,分析了Cisco Packet Tracer软件、"网络系统设计与集成"课程培养目标以及Cisco Packet Tracer支持下的"网络系统设计与集成"创客课程的特点与... 详细信息

近年来,创客教育发展迅速,影响突出,符合时代发展需求。文章以"网络系统设计与集成"课程为例,分析了Cisco Packet Tracer软件、"网络系统设计与集成"课程培养目标以及Cisco Packet Tracer支持下的"网络系统设计与集成"创客课程的特点与优势,依据"以学生为中心"和"做中学"理论,设计了Cisco Packet Tracer支持下的创客教育教学流程,实现了Cisco Packet Tracer支持下的创客教育应用。研究结果表明,Cisco Packet Tracer支持下的"网络系统设计与集成"的创客教育通过协作、分享,在虚拟学习环境中进行创新、实践,能克服传统教学中教学内容抽象、教学形式单一、教学效果不佳的问题;能使学生轻松掌握网络设计与集成方面的知识,能使学生积极主动参与自主学习和协作学习的过程,能提升学生的动手能力、创新意识与创新能力。

关键词： Cisco Packet Tracer 创客教育网络系统设计与集成创客流程应用

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基于Packet Tracer的车辆道闸系统物联网实验设计

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实验技术与管理 2019年第11期36卷 133-136页

作者：范君胡为民蔡彬彬业巧林业宁南京林业大学信息科学技术学院江苏南京210094 江苏工程职业技术学院建筑工程学院江苏南通226007 江苏工程职业技术学院图文中心江苏南通226007

利用Packet Tracer的物联网仿真系统平台,规划和设计了车辆道闸实验系统。系统包括RFID读卡器、车牌显示器、监视器、地感线圈、车辆道闸、通行车辆等物联网实验对象。根据道闸系统工作原理设计了道闸系统物件的硬件接口、网络拓扑、软... 详细信息

利用Packet Tracer的物联网仿真系统平台,规划和设计了车辆道闸实验系统。系统包括RFID读卡器、车牌显示器、监视器、地感线圈、车辆道闸、通行车辆等物联网实验对象。根据道闸系统工作原理设计了道闸系统物件的硬件接口、网络拓扑、软件功能,并通过Python软件编程和IoT服务器逻辑控制编程,实现了道闸系统实验仿真,为物联网实践教学提供了有效、可行的解决思路。

关键词：道闸系统物联网 Packet Tracer Python

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Packet Tracer在“计算机通信与网络”课程理论教学中的应用研究

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中文科技期刊数据库（全文版）教育科学 2022年第10期 0189-0192页

作者：廖伯勋广州工商学院工学院广东广州510850

计算机通信与网络课程的概念术语多,理论抽象,学生难以理解掌握。该课程的理论教学一直是困扰任课教师的一大难题。基于思科模拟器Packet Tracer,结合一个案例,研究如何使用该模拟器软件的模拟模式,实现以实时可控的动画形式来演示网络... 详细信息

计算机通信与网络课程的概念术语多,理论抽象,学生难以理解掌握。该课程的理论教学一直是困扰任课教师的一大难题。基于思科模拟器Packet Tracer,结合一个案例,研究如何使用该模拟器软件的模拟模式,实现以实时可控的动画形式来演示网络协议报文交互过程,即提高了学生的学习兴趣,又能帮助学生理解网络协议的原理,具有较大的参考和应用推广价值。

关键词： Packet Tracer 计算机网络理论教学模拟器

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基于Packet Tracer的BGP路由协议实践教学仿真

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实验室研究与探索 2017年第4期36卷 94-97页

作者：刘昕马顺利昝风彪叶涛青海民族大学计算机学院西宁810007

在西部地区有些高校缺乏网络实验设备,很难满足网络工程专业的高级路由技术课程中实践教学的需求,提出了一种在Packet Tracer模拟环境下实现BGP路由协议的配置,并对实践结果进行了验证。通过仿真实验,可以降低实验投资成本,是一种可行... 详细信息

在西部地区有些高校缺乏网络实验设备,很难满足网络工程专业的高级路由技术课程中实践教学的需求,提出了一种在Packet Tracer模拟环境下实现BGP路由协议的配置,并对实践结果进行了验证。通过仿真实验,可以降低实验投资成本,是一种可行的、廉价的高效解决方案。

关键词： Packet Tracer 路由协议模拟 BGP

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Biotinylated dextran amine is an ideal anterograde Tracer for the corticospinal tract in a goat model of ischemic corticospinal tract injury

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Neural Regeneration Research 2011年第25期6卷 1960-1964页

作者： Xiaoyu Yang Yufei Gao Zhigang Qin Xia Cao Xinquan Gu China-Japan Union Hospital Jifin University Changchun 130033 Jilin Province China School of Pharmaceutical Sciences Jilin University Changchun 130021 Jifin Province China

Existing visualized Tracer studies of the corticospinal tract have been focused on rodents, which have markedly different spinal cord structures compared with humans. In this study, the segmental artery feeding the spinal cord was embolized with digital subtraction angiography to establish a goat model of ischemic spinal cord injury. Biotinylated dextran amine was injected into the motor function areas of the cortex in goats with ischemic spinal cord injury. The corticospinal tract originates from the cerebral cortex motor function area, and travels towards the lateral funiculus at the contralateral spinal dorsal horn after decussation at the pyramid. The number of corticospinal tract positive fibers was found to be gradually reduced. These findings indicate that digital subtraction angiography can be applied to a goat model of ischemic spinal cord injury. Biotinylated dextran amine visualizes the course of the goat corticospinal tract in the spinal cord, which is similar to the human spinal cord. Biotinylated dextran amine is an ideal Tracer for the corticospinal tract.

关键词： corticospinal tract biotinylated dextran amine ischemic spinal cord injury digital subtraction angiography Tracer neuroanatomy neural regeneration

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基于Packet Tracer的校园网设计与仿真

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实验室研究与探索 2017年第10期36卷 127-130,157页

作者：张鹤飞云红艳张德祥井文英钱成功青岛大学计算机科学技术学院山东青岛266071 青岛大学智慧校园与信息化建设中心山东青岛266071

小型园区网设计规划是计算机网络综合技能训练的重要实验内容,分析校园网构建的关键技术,采用Cisco Packet Tracer软件设计仿真校园网络拓扑规划、网络设备选型、网络设备互联配置命令,实现校园网的基本功能。教学实践表明,采用Packet T... 详细信息

小型园区网设计规划是计算机网络综合技能训练的重要实验内容,分析校园网构建的关键技术,采用Cisco Packet Tracer软件设计仿真校园网络拓扑规划、网络设备选型、网络设备互联配置命令,实现校园网的基本功能。教学实践表明,采用Packet Tracer仿真软件提高了学生网络实验设计的兴趣,综合设计案例教学增强了学生分析解决问题的能力,培养了学生的创新思维,在实践教学中取得了较好的教学效果。

关键词：校园网 Cisco Packet Tracer 配置仿真

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