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Single-cell transcriptomic Atlas of aging macaque ocular outflow tissues

Protein & Cell 2024年第8期15卷 594-611页

作者： Jian Wu Chaoye Wang Shuhui Sun Tianmin Ren Lijie Pan Hongyi Liu Simeng Hou Shen Wu Xuejing Yan Jingxue Zhang Xiaofang Zhao Weihai Liu Sirui Zhu Shuwen Wei Chi Zhang Xu Jia Qi Zhang Ziyu Yu Yehong Zhuo Qi Zhao Chenlong Yang Ningli Wang Beijing Institute of Ophthalmology Beijing Tongren Eye CenterBeijing Tongren HospitalCapital Medical UniversityBeijing Key Laboratory of Ophthalmology and Visual SciencesBeijing 100730China State Key Laboratory of Ophthalmology Zhongshan Ophthalmic CenterSun Yat-sen UniversityGuangzhou 510060China State Key Laboratory of Oncology in South China Collaborative Innovation Center for Cancer MedicineSun Yat-sen University Cancer CenterGuangzhou 510060China State Key Laboratory of Membrane Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China Beijing Institute for Stem Cell and Regenerative Medicine Beijing 100101China Institute for Stem Cell and Regeneration Chinese Academy of SciencesBeijing 100101China Department of Neurosurgery Peking University Third HospitalCenter for Precision Neurosurgery and Oncology of Peking University Health Science CenterBeijing 100191China Spencer Center for Vision Research Byers Eye InstituteSchool of MedicineStanford UniversityPalo AltoCA 94304USA

The progressive degradation in the trabecular meshwork(TM)is related to age-related ocular diseases like primary open-angle ***,the molecular basis and biological significance of the aging process in TM have not been fully ***,we established a dynamic single-cell transcriptomic landscape of aged macaque TM,wherein we classified the outflow tissue into 12 cell subtypes and identified mitochondrial dysfunction as a prominent feature of TM ***,we divided TM cells into 13 clusters and performed an in-depth analysis on cluster O,which had the highest aging score and the most significant changes in cell proportions between the two ***,we found that the APOE gene was an important differentially expressed gene in cluster O during the aging process,highlighting the close relationship between cell migration and extracellular matrix regulation,and TM *** work further demonstrated that silencing the APOE gene could increase migration and reduce apoptosis by releasing the inhibition on the PI3K-AKT pathway and downregulating the expression of extracellular matrix components,thereby increasing the aqueous outflow rate and maintaining intraocular pressure within the normal *** work provides valuable insights for future clinical diagnosis and treatment of glaucoma.

关键词： single-cell transcriptomic atlas macaque trabecular meshwork ocular outflow tissue aging

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Mechanism of selective laser trabeculoplasty:a systemic review

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International Journal of Ophthalmology(English edition) 2024年第5期17卷 963-968页

作者： Yu-Feng Chen Wen Zeng Department of Ophthalmology Zhongnan Hospital of Wuhan UniversityWuhan 430071Hubei ProvinceChina

Although selective laser trabeculoplasty(SLT)is a recognized method for the treatment of glaucoma,the exact changes in the target tissue and mechanism for its intraocular pressure lowing effect are still *** purpose of this review is to summarize the potential mechanisms of SLT on trabecular meshwork both in vivo and in vitro,so as to reveal the potential mechanism of *** may induce immune or inflammatory response in trabecular meshwork(TM)induced by possible oxidative damage etc,and remodel extracellular *** may also induce monocytes to aggregate in TM tissue,increase Schlemm’s canal(SC)cell conductivity,disintegrate cell junction and promote permeability through autocrine and paracrine *** provides a theoretical basis for SLT treatment in glaucoma.

关键词： mechanisms selective laser trabeculoplasty glaucoma trabecular meshwork

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Development of a novel two color tracer perfusion technique for the hydrodynamic study of aqueous outflow in bovine eyes

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Chinese Medical Journal 2010年第5期123卷 599-605页

作者： ZHU Jing-yin YE Wen GONG Hai-yan Department of Ophthalmology Boston University School of Medicine Boston MA 02118 USA Department of Ophthalmology Huashan Hospital of Fudan University Shanghai 200040 China Department of Ophthalmology Huadong Hospital of Fudan University Shanghai 200040 China

Background Elevation of intraocular pressure is usually associated with primary open angle glaucoma and caused by increased outflow resistance. A two-color fluorescent tracer technique was developed to investigate the hydrodynamics of aqueous humor outflow with changing intraocular pressure within the same eye, to better understand the relationship between outflow facility and effective filtration area. Methods Eighteen enucleated bovine eyes were first perfused at 30 mmHg with Dulbecco＇s phosphate-buffered saline containing 5.5 mmol/L D-glucose. After a stable baseline facility, red fluorescent microspheres （0.5 um, 0.002% v/v） were exchanged and perfused. Eyes in the one-color control group （n=6） were immediately perfused with fixative. In the experimental group （n=6）, eyes were perfused with green tracer after intraocular pressure reduced to 7 mmHg, while in the two-color control group （n=6）, eyes were perfused with green tracer with intraocular pressure remaining at 30 mmHg. All 12 eyes were then per＇fusion-fixed. Outflow facility was continuously recorded in all eyes. Confocal images were taken along the inner wall of the aqueous plexus and the percent of the effective filtration length （PEFL; length of inner wall exhibiting tracer labeling/total length of inner wall） was measured. The relationships between outflow facility and PEFL were analyzed statistically. Results No significant differences were found in baseline facilities （***-1） among the three groups （the experimental group： 0.93±0.12; the two-color control group： 0.90±0.19; the one-color control group： 0.98±0.13）. In the experimental group, the outflow facility was significantly higher at 7 mmHg （4.29±1.01） than that at 30 mmHg （1.90±0.67, P 〈0.001）, which corresponded to a significant increase in the PEFL at 7 mmHg （54.70±8.42） from that at 30 mmHg （（11.76±4.56）%, P 〈0.001）. The PEFL labeled by red fluorescent microspheres in the experimental group （（11.76±4.56）%） showed no significant diff

关键词： aqueous humor intraocular pressure trabecular meshwork filtration fluorescent, microspheres perfusion

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