检索结果-南通市图书馆

Effect of persistent high intraocular pressure on microstructure and hydraulic permeability of trabecular meshwork

Chinese Physics B 2015年第5期24卷 606-613页

作者：梅曦任琳许强郑炜刘志成 School of Biomedical Engineering Capital Medical University Beijing Key Laboratory of Fundamental Research on Biomechanics in Clinical Application Shenzhen Key Laboratory for Molecular Imaging Shenzhen Institutes of Advanced Technology Chinese Academy of Sciences

As the aqueous humor leaves the eye, it first passes through the trabecular meshwork （TM）. Increased flow resistance in this region causes elevation of intraocular pressure （IOP）, which leads to the occurrence of glaucoma. To quantitatively evaluate the effect of high IOP on the configuration and hydraulic permeability of the TM, second harmonic generation （SHG） microscopy was used to image the microstructures of the TM and adjacent tissues in control （normal） and high IOP conditions. Enucleated rabbit eyes were perfused at a pressure of 60 mmHg to achieve the high lOP. Through the anterior chamber of the eye, in situ images were obtained from different depths beneath the surface of the TM. Porosity and specific surface area of the TM in control and high IOP conditions were then calculated to estimate the effect of the high pressure on the permeability of tissue in different depths. We further photographed the histological sections of the TM and compared the in situ images. The following results were obtained in the control condition, where the region of depth was less than 55 μm with crossed branching beams and large pores in the superficial TM. The deeper meshwork is a silk-like tissue with abundant fluorescence separating the small size of pores. The total thickness of pathway tissues composed of TM and juxtacanalicular （JCT） is more than 100 p.m. After putting a high pressure on the inner wall of the eye, the TM region progressively collapses and decreases to be less than 40 μm. Fibers of the TM became dense, and the porosity at 34 μm in the high IOP condition is comparable to that at 105 μm in the control condition. As a consequent result, the permeability of the superficial TM decreases rapidly from 120 μm2 to 49.6 μm2 and that of deeper TM decreases from 1.66 μm2 to 0.57 μm2. Heterogeneity reflected by descent in permeability reduces from 12.4 μm of the control condition to 3.74 μm of the high IOP condition. The persistently high IOP makes the TM region colla

关键词： trabecular meshwork hydraulic permeability intraocular pressure glaucoma

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Apoptosis of Human trabecular meshwork Cells Induced by Transforming Growth Factor-β_2 in vitro

引用

Journal of Huazhong University of Science and Technology(Medical Sciences) 2004年第1期24卷 87-89,94页

作者：曹阳魏厚仁 Pfaffl Michael 笪邦红李忠玉 Department of Ophthalmology Institute of Physiology Department of Emergency Union Hospital Department of Anatomy

Whether transforming growth factor β 2 (TGF β 2) induces apoptosis of human trabecular meshwork cells was investigated in vitro . Cultured 3 5 passage human trabecular meshwork cells were treated with 0 (control), 0.32, 1, 3.2 ng/ml TGF β 2 for 48 h and divided into control group and experimental group. The apoptosis of human trabecular meshwork cells was examined by transmisson electron microscopy, TUNEL technique and flow cytometry. The results showed characteristic morphologic changes of apoptotic cells were observed under transmission electron microscopy. DNA fragmentation of human trabecular meshwork cells was found by TUNEL technique. Quantitative analysis of flow cytometry showed that percentages of apoptotic human trabecular meshwork cells were (2.79±0.44) %, (4.43 ±1.17) % and (9.60±2.05) % respectively with different concentrations [1 ng/ml ( Ptrabecular meshwork cells in vitro and may be involved in the decrease of trabecular meshwork cells in the patients with primary open angle glaucoma and aging of normal people.

关键词： transforming growth factor human trabecular meshwork cell, cultured cell death

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Changes in Schlemm’s canal,trabecular meshwork,and relevant parameters in the early stage after SMILE of myopia patients

引用

International Journal of Ophthalmology(English edition) 2022年第2期15卷 291-298页

作者： Dan-Dan Yang Zhi-Qi Chen Wei Chen He Yin Jing-Kai Peng Jun-Ming Wang Department of Refractive Surgery Wuhan Eye Good Ophthalmic HospitalWuhan 430014Hubei ProvinceChina Department of Ophthalmology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030Hubei ProvinceChina

AIM:To observe the changes in Schlemm’s canal(SC),trabecular meshwork(TM),and anterior chamber relevant parameters after small incision lenticule extraction(SMILE) of myopia ***:A total of 58 eyes from 30 patients who underwent SMILE were divided into a low and moderate myopia group(group A,32 eyes) and a high myopia group(group B,26 eyes).The diameter and area of the SC,the width and thickness of TM obtained by CIRRUS HD-OCT5000,and the related anterior chamber parameters obtained by Pentacam anterior segment analysis system,accommodation amplitude(AMP) were observed pre-and *** preoperative intraocular pressure(IOP) and postoperative correction of intraocular pressure(IOPcc) were ***:The diameter and area of the SC in the two groups were significantly increased postoperatively(all P0.05).The corneal curvature,central anterior chamber depth,and anterior chamber volume decreased after SMILE surgery(all P<0.01).There was a weak negative correlation between the SC area change and AMP change in group A(r=-0.362,P<0.01).The postoperative IOP decreased after correction by Shah formula(P<0.05).CONCLUSION:SC and TM in myopia patients change in the early postoperative stage of SMILE and the IOP is decline.

关键词： Schlemm’s canal trabecular meshwork intraocular pressure myopia small incision lenticule extraction

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Effect of Transforming Growth Factor-β_2 on Phagocytosis in Cultured Bovine trabecular meshwork Cells

引用

Journal of Huazhong University of Science and Technology(Medical Sciences) 2001年第4期21卷 318-320页

作者：曹阳魏厚仁笪邦红黄毅 Department of Ophthalmology Xiehe Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan

The effect of transforming growth factor β 2 (TGF β 2) on phagocytosis in bovine trabecular meshwork cells in vitro was investigated. After the cultured bovine trabecular meshwork cells were treated with 0 ng/ml, 0.32 ng/ml, 1 ng/ml, 3.2 ng/ml TGF β 2 for 24 h, latex beads were added into the incubation medium, and the numbers of the latex beads in 20 adjacent cells were counted under a microscope 24 h later, after treatment with Wright's stain. Our results showed that the average numbers of the latex beads in the trabecular meshwork cells treated with TGF β 2 of different concentrations were 53.1±1.7 beads/cell, 56.4±2.9 beads/cell and 77.9±6.5 beads/cell respectinvely, in comparison with 45.5±3.3 beads/cell of the control group. TGF β 2 significantly increased the number of the latex beads phagocytosed by cultured bovine trabecular meshwork cells in a dose dependent manner. TGF β 2 could promote the phagocytosis of bovine trabecular meshwork cells in vitro . It may be involved in the cellularity decrease of the trabecular meshwork in the patients of primary open angle glaucoma through promoting the phagocytosis of trabecular meshwork cells.

关键词： transforming growth factor trabecular meshwork cultured cells phagocytosis

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Evaluation of trabecular meshwork-specific promoters in vitro and in vivo using scAAV2 vectors expressing C3 transferase

引用

International Journal of Ophthalmology(English edition) 2023年第8期16卷 1196-1209页

作者： Jun-Kai Tan Ying Xiao Guo Liu Long-Xiang Huang Wen-Hao Ma Yan Xia Xi-Zhen Wang Xian-Jun Zhu Su-Ping Cai Xiao-Bing Wu Yun Wang Xu-Yang Liu Xiamen Eye Center Xiamen UniversityXiamen 361004Fujian ProvinceChina Department of Pathology Hospital of Chengdu University of Traditional Chinese MedicineChengdu 610032Sichuan ProvinceChina Sichuan Provincial Key Laboratory for Human Disease Gene Study Sichuan Provincial People’s HospitalUniversity of Electronic Science and Technology of ChinaChengdu 610072Sichuan ProvinceChina The First Affiliated Hospital of Fujian Medical University Fuzhou 350004Fujian ProvinceChina Beijing FivePlus Molecular Medicine Institute Co. Ltd.Beijing 102600China Shenzhen Key Laboratory of Ophthalmology Shenzhen Eye HospitalSchool of OptometryJinan UniversityShenzhen 518040Guangdong ProvinceChina Department of Ophthalmology Shenzhen People’s Hospitalthe 2nd Clinical Medical CollegeJinan UniversityShenzhen 518020Guangdong ProvinceChina

AIM:To evaluate the potential of two trabecular meshwork(TM)-specific promoters,Chitinase 3-like 1(Ch3L1)and matrix gla protein(MGP),for improving specificity and safety in glaucoma gene therapy based on self-complementary AAV2(scAAV2)vector ***:An scAAV2 vector with C3 transferase(C3)as the reporter gene(scAAV2-C3)was *** scAAV2-C3 vectors were driven by Ch3L1(scAAV2-Ch3L1-C3),MGP(scAAV2-MGP-C3),enhanced MGP(scAAV2-eMGP-C3)and cytomegalovirus(scAAV2-CMV-C3),*** cultured primary human TM cells were treated with each vector at different multiplicities of *** in cell morphology were observed by phase contrast *** stress fibers and Rho GTPases/Rho-associated protein kinase pathway-related molecules were assessed by immunofluorescence staining,real-time quantitative polymerase chain reaction and Western *** vector was injected intracamerally into the one eye of each rat at low and high doses *** vivo green fluorescence was visualized by a Micron III Retinal Imaging *** pressure(IOP)was monitored using a rebound *** responses were evaluated by slit-lamp *** histopathology analysis was examined by hematoxylin and eosin ***:In TM cell culture studies,the vectormediated C3 expression induced morphologic changes,disruption of actin cytoskeleton and reduction of fibronectin expression in TM cells by inhibiting the Rho GTPases/Rhoassociated protein kinase signaling *** the same dose,these changes were significant in TM cells treated with scAAV2-CMV-C3 or scAAV2-Ch3L1-C3,but not in cells treated with scAAV2-eMGP-C3 or *** lowinjected dose,the IOP was significantly decreased in the scAAV2-Ch3L1-C3-injected eyes but not in scAAV2-MGPC3-injected and scAAV2-eMGP-C3-injected *** highinjected dose,significant IOP reduction was observed in the scAAV2-eMGP-C3-injected eyes but not in scAAV2-MGP-C3-injected *** t

关键词： self-complementary AAV2 chitinase 3-like 1 matrix gla protein trabecular meshwork C3 transferase

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Antagonistic Effects of Tranilast on Proliferation and Collagen Synthesis Induced by TGF-β_2 in Cultured Human trabecular meshwork Cells

引用

Journal of Huazhong University of Science and Technology(Medical Sciences) 2004年第5期24卷 490-492,496页

作者：笪邦红曹阳魏厚仁陈志新水迎波李忠玉 Department of Emergency Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 China Department of Ophthalmology Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 China Department of Ophthalmology Hanchuan TCM Hospital Hanchuan 432300 China Department of Ophthalmology and Visual Sciences Washington University School of Medicine St. Louis 63110 United States Department of Anatomy Tongji Medical College of Huazhong University of Science and Technology Wuhan 430030 China

Whether tranilast had antagonistic effect on proliferation inhibition and collagen synthesis promotion induced by TGF-β 2 in cultured human trabecular meshwork cells was investigated. Suspension of 1×104 cultured human trabecular meshwork cells of 3—5 passage was distributed in each well of a 96-well disk and divided into control group and experimental group. After 24 h, 0 μg/ml (control), 12.5 μg/ml, 25 μg/ml, 50 μg/ml tranilast with 3.2 ng/ml TGF-β 2 were added into the incubation medium. Another 24 h later, proliferation and collagen synthesis in cultured human trabecular meshwork cells were examined respectively by using tetrazolium-based semiautomated colormetric (MTT) assay and 3H-proline incorporation with liquid scintillation technique. The results showed absorbance (A) values of the experimental groups were 0.9036±0.3017, 1.1361± 0.1352, 1.2457±0.1524 according to the different concentrations of tranilast, and 0.8956± 0.1903 of the control group. In comparison with the control group, 25 μg/ml (q'=3.23, Ptrabecular meshwork cells. In comparison with the control group [817.37±124.21 cpm/104 cells], 12.5 μg/ml (620.33±80.46 cpm/104 cells, q'= 4.26, Ptrabecular meshwork cells promoted by TGF-β 2 in a dose-dependent manner. It was concluded that tranilast had the antagonistic effect on the proliferation inhibition and collagen synthesis promotion induced by TGF-β 2 in the cultured human trabecular meshwork cells.

关键词： transforming growth factor human trabecular meshwork cultured cell proliferation collagen

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Gene transfer to human trabecular meshwork cells in vitro and ex vivo using HIV-based lentivirus

引用

International Journal of Ophthalmology(English edition) 2014年第6期7卷 924-929页

作者： Yan Xiang Bin Li Jun-Ming Wang Gui-Gang Li Hong Zhang Anne Manyande Xue-Bi Tian Department of Ophthalmology Tongji HospitalTongji Medical CollegeHuazhong University of Science and Technology School of Psychology Social Work and Human SciencesUniversity of West London Department of Anesthesiology Tongji HospitalTongji Medical CollegeHuazhong University of Science and Technology

AIM: To investigate whether the enhanced green fluorescent protein(EGFP) reporter gene could be transferred into human trabecular meshwork(HTM) cells by a HIV-based lentivirus both in vitro and ex ***: The HIV-based lentivirus that contains an EF1-α promoter driving EGFP expression cassette was constructed following the standard molecular cloning methods. The cultured HTM cells were transduced at a range of multiplicity of infection(MOI) with HIV-based lentivirus. EGFP positive cell populations were detected by flow cytometry. Human anterior eye segments were cultured with perfusion culture system and transfected by HIV-based lentivirus with a 1 ×108transducing unit(TU) virus in perfusion liquid. The intraocular pressure was recorded every 8h for 21 d. The expression of EGFP in the anterior segment of the human eye was detected by fluorescence microscopy. Furthermore, the distribution of EGFP expression was confirmed by anti-EGFP immunohistochemical ***: The HIV-based lentivirus which contains an EF1-α promoter driving EGFP expression cassette was constructed successfully. After HTM cells were transduced with HIV-based lentivirus containing EGFP in vitro, the ratio of EGFP positive cells to the total cell number reached 92.3%, with the MOI of 15. After the lentivirus containing EGFP were used to transduce human anterior eye segments, the EGFP could be directly detected by fluorescence microscopy in *** staining revealed that 88.19%EGFP-positive trabecular meshwork(TM) cells were observed in the human anterior segment. Nevertheless,the intraocular pressure in the lentivirus-transduced group kept constant when compared with control group(P >0.05).CONCLUSION: EGFP gene could be efficiently transferred into HTM cells both in vitro and ex vivo by using HIV-based lentivirus.

关键词： gene transfer trabecular meshwork HIV-based lentivirus glaucoma

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Extracellular matrix gene expression in human trabecular meshwork cells following mechanical fluid flow stimulation

引用

International Journal of Ophthalmology(English edition) 2022年第3期15卷 388-393页

作者： Koichi Yoshida Motofumi Kawai Tsugiaki Utsunomiya Akihiro Ishibazawa Young-Seok Song Mariana Sayuri BUdo Yoshikazu Tasaki Akitoshi Yoshida Department of Ophthalmology Asahikawa Medical UniversityAsahikawaHokkaido 078-8510Japan Department of Hospital Pharmacy&Pharmacology Asahikawa Medical UniversityAsahikawaHokkaido 078-8510Japan

AIM: To investigate changes in extracellular matrix(ECM) gene expression in human trabecular meshwork(HTM) cells in response to mechanical fluid flow ***: HTM cells were grown on a glass plate coated with 0.02% type Ⅰ collagen(COL) and exposed to shear stress(0, 0.2, 1.0 dyne/cm;) for 12 *** in genes related to the ECM were evaluated by real-time reverse transcriptase-polymerase chain *** of Smad2 protein was investigated by Western ***: After mechanical stimulation, COL type 4 alpha 2, COL type 6 alpha 1, and fibronectin-1 mRNA were significantly higher than the static control(Ptrabecular meshwork may promote ECM turnover and contribute to intraocular pressure homeostasis.

关键词： trabecular meshwork shear stress aqueous humor extracellular matrix glaucoma intraocular pressure

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Endothelial nitric oxide synthase deficiency influences normal cell cycle progression and apoptosis in trabecular meshwork cells

引用

International Journal of Ophthalmology(English edition) 2016年第6期9卷 799-803页

作者： Qiong Liao Yan-Ming Huang Wei Fan Chan Li Hong Yang Department of Ophthalmology Xinqiao Hospital of Third Military Medical University Department of Ophthalmology the First Affiliated Hospital of Chongqing Medical University of Chongqing Medical University Department of Ophthalmology Southwest HospitalThird Military Medical University

AIM： To clarify how the endothelial nitric oxide synthase （eNOS, NOS3） make effect on outflow facility through the trabecular meshwork （TM）. METHODS： Inhibition of NOS3 gene expression in human TM cells were conducted by three siRNAs. Then the mRNA and protein levels of NOS3 in siRNA-treated and negative control （NC） cells were determined, still were the collagen, type IV, alpha 1 （COL4A1） and fibronectin 1 by real-time PCR and Western blot analysis. In addition, NOS3 concentrations in culture supernatant fluids of TM cells were measured. Cell cycle and cell apoptosis analysis were performed using flow cytometry. RESULTS： The mRNA level of NOS3 was decreased by three different siRNA interference, similar results were obtained not only of the relative levels of NOS3 protein, but also the expression levels of COL4A1 and fibronectin 1. The number of cells in S phase was decreased, while contrary result was obtained in G2 phase. The number of apoptotic cells in siRNA-treated groups were significant increased compared to the NC samples. CONCLUSION： Abnormal NOS3 expression can make effects on the proteins levels of extracellular matrix component （e.g. fibronectin 1 and COL4A1）. Reduced NOS3 restrains the TM cell cycle progression at the G2/ M-phase transition and induced cell apoptosis.

关键词： endothelial nitric oxide synthase cell cycle cell apoptosis trabecular meshwork

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Existence of Heme Oxygenase-carbon Monoxide-cyclic Guanosine Monophosphate Pathway in Human trabecular meshwork Cells In Vitro

引用

Journal of Huazhong University of Science and Technology(Medical Sciences) 2004年第2期24卷 173-177页

作者：李涛张虹梁峰 Department of Ophthalmology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Department of Chemistry Wuhan University

To confirm the existence of heme oxygenase (HO)-carbon monoxide (CO)- cyclic guanosine monophosphate (cGMP) pathway in the cultured human trabecular meshwork cells (HTMCs) in vitro, and to evaluate the inductive role of hemin on this pathway, HTMCs of the third to fourth generation were cultured in vitro. Reverse transcripase-polymerase chain reaction (RT-PCR) was employed for detection of HO-1 and HO-2 mRNA. Immunohistochemical staining was used to detect HO-1 and HO-2 proteins. Hemin was added into the culture solution. The HO-1 mRNA levels were quantified by RT-PCR. The relative amount of carbon monoxide released into the media was measured with the quantifying carbon monoxide hemoglobin (HbCO) by spectrophotometry. Radioimmunoassay was used to determine changes of cGMP in HTMCs. The results showed that cultured cells had the specific characteristics of HTMCs. Both HO-1 and HO-2 genes were expressed in HTMCs, as well as HO-1 and HO-2 proteins in HTMCs. Hemin induced HO-1 mRNA, HbCO and cGMP in a dose-dependent manner. In conclusion, HO-CO-cGMP pathway exists in the cultured HTMCs and can be induced by hemin. Pharmacological stimulation of HO-CO-cGMP pathway may constitute a novel therapeutic approach to rescuing glaucoma.

关键词： trabecular meshwork cell culture heme oxygenase carbon monoxide guanosine 3', 5'-cyclic monophosphate (cGMP)

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：