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Measurement of total phenolic content and antioxidant activity of aerial parts of medicinal plant Coronopus didymus

Asian Pacific Journal of Tropical Medicine 2017年第8期10卷 855-862页

作者： Hafiza Noreen Nabil Semmar Muhammad Farman James S.O.McCullagh Department of Chemistry Quaid-i-Azam UniversityIslamabad 45320Pakistan Institut Supérieur des Sciences Biologiques Appliquées de Tunis Tunisia Chemistry Research Laboratory Department of ChemistryUniversity of OxfordMansfield RoadOxford OX1 3TAUnited Kingdom

Objective: To evaluate the total phenolic content and compare the antioxidant activity of various solvent extracts and fractions from the aerial parts of Coronopus didymus through various assays. Methods: total phenolic content was determined using the Folin-Ciocalteu assay and the in vitro antioxidant activity of a number of different extracts was investigated in a dose-dependent manner with three different methods: the 2,2-diphenyl-1-picrylhydrazyl(DPPH), 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt(ABTS) and ferric reducing antioxidant power(FRAP) assays. A flavone was isolated from the most active ethanolic extract with high antioxidant activity using size exclusion chromatography. IC_(50) values were calculated for the DPPH and ABTS methods. The FRAP activity was assessed in terms of μM Fe(II) equivalent. Results: The phenolic content was found to be highest in the ethanol extract(CDA Et; 47.8 mM GAE) and the lowest in the dichloromethane extract(CDA DCM; 3.13 mM GAE). The ethanol extract showed high radical scavenging activity towards DPPH and ABTS radicals with IC_(50) values of(7.80 × 10~2) and(4.32 × 10~2) μg/m L, respectively. The most active ethanol extract had a FRAP value of 1 921.7 μM Fe(Ⅱ) equivalent. The isolated flavone F10C(5,7,4'-trihydroxy-3'-methoxy flavone) was far more effective for scavenging free radicals in the DPPH and ABTS assays with IC_(50) of 43.8 and 0.08 μg/m L, than the standard trolox, with IC_(50) values of 97.5 and 21.1 μg/m L, respectively. In addition, the flavone F10C and the standard ascorbic acid had FRAP values of 1 621.7 and 16 038.0 μM Fe(Ⅱ) equivalents, respectively. Conclusions: The total phenolic content of extracts in decreasing order is ethanol extract(CDA Et)> acetone extract(CDA ACE)> phenolic extract(CDA MW)> n-hexane extract(CDA nHX)> chloroform extract(CDA CHL)> dichloromethane extract(CDA DCM). The ordering of extracts in terms of antioxidant activity from highest to lowest is CDA Et> C

关键词： Coronopus didymus Antioxidant activity total phenolic content Size exclusion chromatography

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Comparison of total phenolic content and total Antioxidant Activity in Local Red Wines Determined by Spectrophotometric Methods

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Food and Nutrition Sciences 2014年第17期5卷 1660-1667页

作者： Ebru Büyüktuncel Esra Porgali Cemil Colak Department of Analytical Chemistry Faculty of PharmacyUniversity of InonuMalatyaTurkey Department of Biostatistics Faculty of MedicineInonu UniversityMalatyaTurkey

In this study, the antioxidant activity of local red wines was determined by four different analytical methods: 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay, 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS?+) radical cation decolourisation, ferric reducing/antioxidants power (FRAP) and cupric ion reducing capacity in the presence of neocuproine (CUPRAC) for the evaluation of reducing power (PR). They were correlated with the total phenolic content (TPC) determined with the Folin-Ciocalteu’s reagent using gallic acid as a standard. The total polyphenol concentration was found to vary from 2599.90 to 4846.57 mg/L gallic acid equivalents (GAE). The total antioxidant activity determined by DPPH, ABTS, FRAP and CUPRAC methods was found to vary from 7.49 - 15.93 mmol/L, 12.02 - 24.73 mmol/L, 12.65 - 27.68 mmol/L and 13.19 - 31.07 mmol/L, respectively. The total phenolic contents of red wine samples exhibited a good correlation (p < 0.01) with antioxidant properties.

关键词： Red Wine Antioxidant Activity total phenolic content DPPH ABTS FRAP CUPRAC

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Antioxidant,antimicrobial and total phenolic contents of Calophyllum symingtonianum

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Journal of Coastal Life Medicine 2013年第1期 50-54页

作者： Nissad Attoumani Deny Susanti Muhammad Taher Department of Biotechnology Faculty of ScienceInternational Islamic University MalaysiaJalan Sultan Ahmad ShahBandar Indera Mahkota25200 KuantanPahangMalaysia Department of Pharmaceutical Technology Faculty of PharmacyInternational Islamic University MalaysiaJalan Sultan Ahmad ShahBandar Indera Mahkota25200 KuantanPahangMalaysia

Objective:To determine the total phenolic content,antioxidant and antimicrobial activities of the extracts from Calophyllum ***:The extracts were tested for their antioxidant activity by the DPPH radical scavenging assay and the β-carotene bleaching assay,while the antimicrobial activity was determined by disc diffusion ***:All the tested extracts showed antioxidant and antimicrobial *** extracts showed moderate antimicrobial activity against Staphylococcus aureus with zone of inhibition values of 10,11 and 12 mm for n-hexane,dichloromethane and methanol extracts respectively at 30μg/disc.N-hexane showed low antimicrobial activity against Pseudomonas aeruginosa(5 mm)at 30μg/*** total phenolic test showed that methanol has high phenolic content(162.25 mg GAE/g of extract)compared to the other ***:The ability of the extracts to inhibit microbial growth at a concentration of 30μg/disc indicated the its potent antimicrobial activity.

关键词： Calophyllum symingtonianum Antioxidant Antimicrobial total phenolic content

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Nutrients and anti-nutrients of high chlorophyll-mungbean sprouts as affected by different periods of germination and sprouting stages

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International Journal of Agricultural and Biological Engineering 2013年第4期6卷 121-129页

作者： Benjaruk Vayupharp Varaporn Laksanalamai Faculty of Biotechnology Rangsit UniversityMuang-AkePhatumthani 12000Thailand

The variation of nutrient and anti-nutrient compounds in commercial mungbean cultivars(Chinat 72,MS-1,Chinat 80,and L3-8)during seven periods of germination and sprouting was *** seeds were selected randomly at 6 h of soaking(1^(st)stage),23 h(2^(nd)stage),47 h(3^(rd)stage),71 h(4^(th)stage),77 h(5^(th)stage)of sprouting,and 12 h,and 24 h of sunshine exposing(6th and 7th stage,respectively).It was found that nutrition compositions(including protein content,crude fiber content,vitamin C content,total minerals,and HCL-extractability of minerals)of all cultivars significantly increased with germination and *** the last stage,the total phenol was the highest amount which was not significantly different from all *** total antiradical capacity(%,DPPH inhibition)increased up to the maximum value in the last two stages of *** results showed that the phytic acid,the anti-nutrient component decreased with the consequence of germination,and reached the untraceable value at the last *** addition,the highest amount of chlorophyll(7.15-8.99 mg/100 g)was found in Chinat 72 and MS-1 cultivars at the last stage of sprouting,comparing to Chinat 80 and L3-8 *** is therefore recommended to consume high chlorophyll mungbean sprout with the benefits of high nutrient constituents and low price purchase comparing to other green vegetables.

关键词： high chlorophyll phytic acid total phenolic content radical scavenging activity HCl extractability of minerals mungbean sprouts

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Seasonal variation and ecological importance of tannin and nutrient concentrations in Casuarina equisetifolia branchlets and fine roots

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Journal of Forestry Research 2020年第5期31卷 1499-1508页

作者： Lihua Zhang Shangju Zhang Gongfu Ye Xiaochun Qin School of Biological Science and Technology University of JinanJinan 250022People’s Republic of China Key Laboratory of Coastal Environmental Processes and Ecological Remediation Yantai Institute of Coastal Zone Research(YIC)Chinese Academy of Sciences(CAS)Shandong Provincial Key Laboratory of Coastal Environmental ProcessesYICCASYantai 264003People’s Republic of China Pingtan State-Owned Protection Forest Farm of Fujian Province Pingtan 350400People’s Republic of China Fujian Academy of Forestry Fuzhou 350012People’s Republic of China

In this study,we investigated the effects of environmental factors on plant phenolic variability,seasonal dynamics of total phenolic content(TP),extractable condensed tannins(ECT),protein-bound condensed tannins(PBCT),fiber-bound condensed tannins(FBCT),total condensed tannins(TCT),protein precipitation capacity(PPC)and nutrient content in the branchlets and fine roots of Casuarina *** and TCT concentrations in branchlets were lowest in the spring,then increased in summer and autumn,similar to the seasonal dynamics in air *** and TCT concentrations in fine roots were highest in summer,coinciding with heavy *** general,TP and TCT concentrations were higher in branchlets than in fine *** significant difference was found in C concentration among various seasons for either branchlets or fine *** had significantly higher N and P concentrations than fine roots in most *** C/N and N/P ratios in branchlets were significantly lower than in fine roots in all seasons,except *** relationship between branchlets and fine roots was significant for C,P and FBCT,but no significant relationships were found for N,TP,ECT,PBCT and ***,TP and TCT content were each significantly correlated with PPC in branchlets and in fine *** TP/N and TCT/N ratios were highest in the autumn for the branchlets and in the summer for fine *** results indicate that high temperatures lead to increased tannin production in branchlets,but that the tannin content in fine roots is mainly affected by *** content was greater in branchlets than in fine roots,which may indicate that selective pressure is greater on branchlets than on fine roots.

关键词： Casuarina equisetifolia Condensed tannins total phenolic content Nutrient Seasonal variation

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Fruit Yield and Quality, and Irrigation Water Use Efficiency of Summer Squash Drip-Irrigated with Different Irrigation Quantities in a Semi-Arid Agricultural Area

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Journal of Integrative Agriculture 2014年第11期13卷 2518-2526页

作者： Yasemin Kuslu Ustun Sahin Fatih M Kiziloglu Selcuk Memis Department of Agricultural Structures and Irrigation Atatürk University

Fruit yield, yield components, fruit mineral content, total phenolic content, antioxidant activity and irrigation water use efifciency （IWUE） of summer squash responses to different irrigation quantities were evaluated with a ifeld study. Irrigations were done when the total evaporated water from a Class A pan was about 30 mm. Different irrigation quantities were adjusted using three different plant-pan coefifcients （Kcp, 100% （Kcp1）, 85% （Kcp2） and 70% （Kcp3））. Results indicated that lower irrigation quantities provided statistically lower yield and yield components. The highest seasonal fruit yield （80.0 t ha-1） was determined in the Kcp1 treatment, which applied the highest volume of irrigation water （452.9 mm）. The highest early fruit yield, average fruit weight and fruit diameter, length and number per plant were also determined in the Kcp1 treatment, with values of 7.25 t ha-1, 264.1 g, 5.49 cm, 19.95 cm and 10.92, respectively. Although the IWUE value was the highest in the Kcp1 treatment （176.6 kg ha-1 mm-1）, it was statistically similar to the value for Kcp3 treatment （157.1 kg ha-1 mm-1）. total phenolic content and antioxidant activity of fruits was higher in the Kcp1 （44.27 μg gallic acid equivalents （GAE） mg-1 fresh sample） and in the Kcp2 （84.75%） treatments, respectively. Major （Na, N, P, K, Ca, Mg and S） and trace （Fe, Cu, Mn, Zn and B） mineral contents of squash fruits were the highest in the Kcp2 treatment, with the exception of P, Ca and Cu. Mineral contents and total phenolic content were signiifcantly affected by irrigation quantities, but antioxidant activity was not affected. It can be concluded that the Kcp1 treatment was the most suitable for achieving higher yield and IWUE. However, the Kcp2 treatment will be the most suitable due to the high fruit quality and relatively high yield in water shortage conditions.

关键词： summer squash drip irrigation irrigation water use efifciency total phenolic content antioxidant activity fruit mineral content

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Diversity of Antioxidant content and Its Relationship to Grain Color and Morphological Characteristics in Winter Wheat Grains

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Journal of Integrative Agriculture 2014年第6期13卷 1258-1267页

作者： MA Dong-yun SUN De-xiang ZUO Yi WANG Chen-yang ZHU Yun-ji GUO Tian-cai National Engineering Research Center for Wheat Henan Agricultural University

The current interest in the health beneifts of whole wheat grain has prompted breeders to further increase the concentration of antioxidants in wheat. The objective of this study was to investigate the variation in antioxidant content among Chinese wheat grains and the relationship between antioxidants and grain color and morphological characteristics. A wide variation was observed in the total phenolic, carotenoid and lfavonoid contents, as well as the antioxidant activity （AOA）, of Chinese wheat varieties. Black wheat had the highest mean total phenolic, carotenoid and lfavonoid contents and the highest AOA, followed by red and white wheats. The grain color parameters were signiifcantly negatively correlated with total phenolic, carotenoid and lfavonoid contents and AOA among all of the wheat varieties examined, and grain weight was also signiifcantly negatively correlated with these traits. The same correlation between grain weight and antioxidant traits was also observed within individual groups of wheat, which indicates that grain weight may be used as an index for selecting wheat varieties with high AOA. Landraces had signiifcantly higher lfavonoid content than commercial wheat varieties. The results of this study may be useful for breeding nutrient-rich wheat varieties.

关键词： wheat total phenolic content antioxidant activity flavonoid carotenoid

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Dynamic Changes in Enzyme Activities and phenolic content during Early Stage of Explants Browning in Herbaceous Peony(Paeonia lactiflora)

Dynamic Changes in Enzyme Activities and Phenolic Content du...

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2017年中国作物学会学术年会

作者： Masocha Valerie Farai Ge Shujun College of Agronomy Hebei Agricultural University/North China Key Laboratory for Crop Germplasm Resources of Education Ministry

[Research background]Paeonia lactiflora Pall,a herbaceous peony is a well-known traditional plant in China as it is widely used in landscaping and garden greening(high ornamental value) due to its large flower,elegant shape,gorgeous color and rich fragrance,and it has extensively expanded out of *** only are herbaceous peonies grown as garden ornamentals,but they also have a long history of use as traditional medicinal *** is clinically used to treat depression-like *** the popularity of this ornamental and medicinal plant,several reasons restrict its production and use,such as lack of reliable systems for rapid *** multiple traditional choices for propagation of peony have low propagation coefficiency which makes it difficult to meet the increasing demands of the global *** vitro culture is considered to be an effective vegetative propagation means for peony production through mass clonal multiplication since it possesses many ***,explant browning due to accumulation of phenolic compounds is one of the major problems in the primary culture and polyphenol oxidase and phenylalanine ammonia lyases(PAL) are major proteases involved in plant tissue *** related studies were less reported on this species.[Materials and methods]Unspecified cultivars widely grown in most parts of China were used as initial plant material for the *** the materials bought from local medicinal germplasm market(Anguo,Hebei) were planted in pots and placed outside for natural environmental *** dynamic changes of phenolic content and the two major proteases activities were assessed during the early stages of explants browning across summer,autumn,winter and spring *** several measure were tried to control the browning of the explants.[Results and analysis]There were statistically significant differences in both phenolic contents and enzyme activities across the four different seasons at 5%significance

关键词： Paeonia lactijlora Pall Explants browning total phenolic content Enzyme activities Dynamic changes Activated charcoal

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Preparation and Characterization of Two Wood Vinegars Obtained from Hull of Spina Date Seed and Shell of Peanut

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Chemical Research in Chinese Universities 2017年第3期33卷 348-353页

作者： LI Zhanchao ZHANG Zhiquan WU Lijie WANG Jichang LIU Zhenheng ZHANG Zhongwei ZHANG Hanqi WANG Ziming College of Chemistry Jilin University Changchun 130012 P. R. China JidongKeda Biomass Energy Technology Co. Ltd. of Songyuan City Songyuan 138000 P. R. China Bureau of Animal Husbandry Songyuan 138000 P. R. China

Two new kinds of wood vinegars（WVs） from the hull of spina date seed（HSDS） and the shell of peanut（PS） were prepared. 32 Major components in the two kinds of WVs were identified and determined by gas chro- matograph-mass spectrometery. The total phenolic content was determined and the antioxidant activity of the WV was measured. The total phenolic contents of PSWV and HSDSWV were 75.9 and 98.1 rag/L, respectively, when they were expressed as catechol equivalents. In 1,1-diphenyl-2-picrylhydrazyl assay, free radical scavenging activity of HSDSWV was also superior than that of PSWV. In addition, the antioxidant activities of the two kind of WVs exhibited a good positive correlation with total phenolic content, and the linear correlation coefficients ranged from 0.9594 to 0.9970. The two kind of WVs could be used in the fields of food and medicine due to their powerful antioxidant activities.

关键词： Hull of spina date seed Shell of peanut Wood vinegar total phenolic content Antioxidant activity Free radical scavenging activity

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Evaluation of antioxidant and anti-hypercholesterolemic potential of Vitis vinifera leaves

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Food Science and Human Wellness 2017年第3期6卷 131-136页

作者： Sushma Devi Randhir Singh Department of Pharmaceutical Sciences Maharishi Markandeshwar UniversityMullanaAmbalaIndia

Background:Vitis vinifera is universally appreciated herb(especially fruit)for their delicacy,nutrition and conventional as functional *** the present study,we evaluated anti-hypercholesterolemic potential of Vitis vinifera red leaves extract in experimental wistar male *** and methods:Qualitative and quantitative phytochemical screening of vitis vinifera methanolic extract(VVME)and vitis vinifera aqueous extract aqueous(VVAE)extract was carried out to identify the *** anti-hypercholesterolemic activity was evaluated by inducing hypercholesterolemia with high cholesterol diet for 21 days in experimental *** the experimental periods parameters like lipid profile,liver function test,atherogenic index and histopathological studies were ***:total tannins,total flavonoids and total phenolic contents were found in major amount in *** lipid levels were significantly attenuated by different doses of VVME and ***,VVME was found more effective than VVAE and histopathological results confirmed the effectiveness of ***:It can be concluded that anti-hypercholesterolemic efficacy of Vitis vinifera might be due to presence of active phytoconstituents and its antioxidant efficacy.

关键词： Vitis vinifera Hypercholesterolemia Cholesterol diet total phenolic content

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