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Enzyme-loaded liposome with biocatalytic precipitation for potentiometric immunoassay of thyroid-stimulating hormone in thyroid carcinoma

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Chinese Chemical Letters 2020年第2期31卷 463-467页

作者： Yongjin Cao Min Zheng Weihua Cai Zongcai Wang Department of thyroid and Breast Surgery The First Affiliated Hospital of Fujian Medical UniversityFuzhou 350005China Department of Medical Oncology The First Affiliated Hospital of Fujian Medical UniversityFuzhou 350005China

A simple and feasible potentiometric immunosensing platform based on enzymatic biocatalytic precipitation technique was designed for the sensitive detection of thyroid-stimulating hormone(TSH;a typical kind of biomarkers for thyroid carcinoma),using horseradish peroxidase(HRP)-loaded liposome for the signal *** construct such an assay system,a sandwich-type immunoreaction was readily carried out on monoclonal anti-TSH capture antibody-coated electrode by using polyclonal antiTSH secondary antibody-conjugated HRP-loaded *** the formation of sandwichtype immunocomplex,the carried liposome was lysed through the added Triton X-100 to release the entrapped HRP molecules,which catalyzed the oxidation of 4-chloro-1-naphthol to produce an insoluble and uncharged organic precipitation on the electrode surface,thereby causing the change of the local electrical *** labeling protocols with and without the liposome were investigated for detection of target TSH,improved analytical features were achieved with HRP-entrapped *** optimal conditions,the potentiometric immunosensor had good responses for TSH detection within the linear range of 0.01-30 p IU/mL at a detection limit of 0.0067 p IU/*** reproducibility,high specificity and long-time stability were acquired during the assay ***,a wellmatched accuracy between the potentiometric immunosensor and commercial human TSH ELISA kit was gave for the analysis of human serum samples.

关键词： Potentiometer Modification electrode Potentiometric immunoassay thyroid-stimulating hormone Enzyme-encapsulated liposome Enzyme biocatalytic precipitation

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thyroid function of twin-pregnant women in early pregnancy

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Chinese Medical Journal 2019年第17期132卷 2033-2038页

作者： Yan-Xin Jiang Wei-Jie Sun Yang Zhang Yu Huang You-Yuan Huang Gui-Zhi Lu Jun-Qing Zhang Ying Gao Hui-Xia Yang Xiao-Hui Guo Department of Endocrinology Peking University First Hospital Beijing 100034 China Department of Obstetrics and Gynecology Peking University First Hospital Beijing 100034 China National Engineering Research Center of Software Engineering Peking University Beijing 100871 China

Background: Serum human chorionic gonadotrophin (hCG) is higher in twin than that in singleton pregnancies. As hCG stimulates the thyroid to produce more free thyroxine (FT4), which may lead to decreased thyroid-stimulating hormone (TSH) levels, the reference ranges of thyroid-related indicators may differ between singleton and twin pregnancies in the first trimester. This study aimed to establish reference ranges for thyroid-related indicators in early twin pregnancies and to compare them with singleton pregnancies. Methods: Data of 820 twin-pregnant women were extracted from the established database of all pregnant women who delivered at Peking University First Hospital from October 2013 to May 2018;160 who met National Academy of Clinical Biochemistry criteria were included to establish TSH and FT4 reference ranges. We screened 480 (3:1 paired) women with singleton pregnancies from the same database as controls. The Mann-Whitney test for TSH and FT4 levels was applied for comparisons between singleton and twin pregnancies. Results: First-trimester reference ranges (4–12 gestational weeks) for twin pregnancies were: TSH 0.69 (0.01–3.35) mIU/L and FT4 16.38 (12.45–23.34) pmol/L. Median TSH was significantly lower at 7 to 12 gestational weeks than that at 4 to 6 gestational weeks (0.62 vs. 0.96 mIU/L, Z =-1.964, P = 0.049);FT4 was not significantly different between the two groups. Compared to singleton pregnancies, median TSH was significantly lower (0.69 vs. 1.27 mIU/L, Z =-6.538, P = 0.000), and FT4 was significantly higher (16.38 vs. 14.85 pmol/L, Z =-7.399, P = 0.000) in twin pregnancies in the first trimester. Conclusions: Specific reference ranges for thyroid-related indicators for twin pregnancies are needed to avoid a misdiagnosis of thyroid dysfunction. Moreover, establishment of separate reference ranges for 4 to 6 and 7 to 12 gestational weeks in twin pregnancies may be considered.

关键词： Twin pregnancy Reference ranges thyroid-stimulating hormone Free thyroxine

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Drug-Induced Hypothyroidism during Anti-Tuberculosis Treatment of Multidrug-Resistant Tuberculosis: Notes from the Field

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Journal of Tuberculosis Research 2016年第3期4卷 105-110页

作者： Somashekar Munivenkatappa Singarajipura Anil Balaji Naik Tyson Volkmann Karuna D. Sagili Jayachamarajapura S. Akshatha Shashidhar Buggi Manchenahalli A. Sharada Sudhendra Kulkarni Vineet K. Chadha Patrick K. Moonan Drug Resistant Treatment Centre Bengaluru India Sri Devarao Shivaram Tuberculosis and Rajiv Gandhi Institute of Chest Diseases Bengaluru India Revised National Tuberculosis Programme-State of Karnataka Bengaluru India World Health Organization India Country Office New Delhi India U.S. Centers for Disease Control and Prevention Atlanta GA USA International Union against Tuberculosis and Lung Disease (The Union) South-East Asia Regional Office New Delhi India National Tuberculosis Institute Bengaluru India

We followed 188 euthyroidic persons undergoing treatment for multidrug resistant tuberculosis (MDR-TB) in the state of Karnataka, India to determine the incidence of hypothyroidism during anti-tuberculosis treatment. Overall, among MDR-TB patients with valid thyroid stimulating hormone (TSH) values, about 23% developed hypothyroidism (TSH value ≥10 mIU/ml) during anti-tuberculosis treatment;the majority (74%) occurring after 3 months of treatment. Among 133 patients who received a regimen that contained ethionamide, 42 (32%) developed hypothyroidism. Among 17 patients that received a regimen that contained para-aminosalicylate sodium, 6 (35%) developed hypothyroidism. Among 9 HIV positive patients on antiretroviral treatment, 4 (44%) developed hypothyroidism. These results differ from previously reported 4% incidence of hypothyroidism amongst patients who passively reported thyroidal symptoms during treatment, suggesting routine serologic monitoring of TSH throughout the course of treatment for MDR-TB is warranted.

关键词： Hypothyroidism Multidrug Resistance Tuberculosis thyroid-stimulating

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Nerve cell apoptosis in patients with post-stroke depression

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Journal of Hainan Medical University 2017年第9期23卷 134-137页

作者： Yun-Jian Ji E Kuang Department of Neurology Fangxian People's Hospital of Shiyan City in Hubei ProvinceShiyan442100China Intensive Care Unit Fangxian People's Hospital of Shiyan City in Hubei ProvinceShiyan442100China

Objective:To study the relationship of serum thyroid-stimulating hormone (TSH) levels with neurotransmitters, neurotrophic state and nerve cell apoptosis in patients with post-stroke depression (PSD).Methods: Patients with ischemic stroke who were treated in our hospital between June 2014 and October 2016 were selected, and according to the HAMD-17 score at 14 days after onset, the enrolled patients were divided into into the post-stroke depression group (PSD group) and stroke group (PS group). Serum levels of TSH, neurotransmitters, neurotrophic cytokines and apoptotic molecules were ***: Serum TSH, 5-HT, NE, DA, BDNF and NGF levels of PSD group were significantly lower than those of PS group while Bax, FasL, Fas and Caspase-3 levels were significantly higher than those of PS group;5-HT, NE, DA, BDNF and NGF levels of PSD group of patients with low TSH were significantly lower than those of PSD group of patients with normal TSH while Bax, FasL, Fas and Caspase-3 levels were significantly higher than those of PSD group of patients with normal ***:The decrease of serum TSH level in patients with PSD can affect neurotransmitters, neurotrophic state and nerve cell apoptosis.

关键词： Post-stroke depression thyroid-stimulating hormone Neurotransmitter Neurotrophic cytokine Apoptosis

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