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Identification and Characterization of a Novel thymus Aging Related Protein Rwdd1

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Cellular & Molecular Immunology 2008年第4期5卷 279-285页

作者： Ning Kang Lian Duan Long Tang Shirong Liu Chunman Li Yonghai Li Qingfeng Liu Yu Hu Lianxian Cui Wei He Department of Immunology School of Basic Medicine Peking Union Medical College and Institute of Basic Medical Sciences Chinese Academy of Medical Sciences Beijing 100005 China

By using DDRT-PCR and EST segment ligation, a novel mouse thymus involution related gene Rwddl was identified. The reading frame encoded a protein of 243 amino acid residues which contained an RWD domain at the N terminus. Rwddl expression in thymus was decreased in aged and oxidatively stressed mice. It was found to be expressed extensively in thymocytes and thymic epithelial cells. The expression level of Rwddl could affect the transactivation activity of androgen receptor （AR） in transiently transfected thymic epithelial cells. However, no direct interaction could be detected by fluorescence resonance energy transfer （FRET） analysis. In conclusion, Rwddl is a thymus involution related protein that may indirectly affect AR signaling pathway. Cellular ＆ Molecular Immunology. 2008;5（4）：279-285.

关键词： Rwdd 1 thymus aging androgen receptor

Achievement of Cellular Immunity and Discordant Xenogeneic Tolerance in Mice by Porcine thymus Grafts

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Cellular & Molecular Immunology 2004年第3期1卷 173-179页

作者： YongZhao ZuyueSun YiminSun AlanN.Langnas TransplantationBiologyResearchDivision StateKeyLabofMembraneandMembraneBiotechnologyInstituteofZoologyChineseAcademyofSciences.Beijing100080China. DepartmentofSurgery.UniversityofNebraskaMedicalCenter OmahaNE68198-7690.USA

Specific cellular immune tolerance may be essential for successful xenotransplantation in humans.Thymecto- mized (ATX),T and NK cell-depleted immunocompetent mice grafted with xenogeneic fetal pig thymic and liver tissue (FP THY/LIV) result in efficient mouse thymopoiesis and peripheral repopulation of functional mouse CD4^+ T cell.Very importantly,the reconstituted mouse T cells are specifically tolerant to pig donor antigens.Studies demonstrated that porcine MHCs mediated positive and negative selection of mouse thymocytes in FP THY grafts,whereas mouse MHCs were involved in negative selection in grafts.Therefore,T cell tolerance to xenogeneic donor antigens could be induced by grafting donor thymus tissue.Xenogeneic thymic replacement might have a potential role in the reconstitution of cellular immunity in patients with AIDS or other immunodeficiencies caused by thymus dysfunction.Cellular & Molecular Immunology.2004;1(3): 173-179.

关键词： xenotransplantation thymus tolerance T cells

Interactive effects of drought stress and chitosan application on physiological characteristics and essential oil yield of thymus daenensis Celak

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The Crop Journal 2017年第5期5卷 407-415页

作者： Zohreh Emami Bistgani Seyed Ataollah Siadat Abdolmehdi Bakhshandeh Abdollah Ghasemi Pirbalouti Masoud Hashemi Ramin Agriculture and Natural Resources University of Khozestan Department of Medicinal Plants Shahrekord Branch Islamic Azad University Stockbridge School of Agriculture University of Massachusetts Amherst Amherst

thymus daenensis, a perennial herb, is often grown in areas that experience drought conditions during its growing period. Application of chitosan may compensate for the negative impact of drought stress on the yield of oil and secondary metabolites in thymus.The interactive effects of foliar application of chitosan and drought stress on dry matter,essential oil yield, and selected physiological characteristics including photosynthetic pigments, osmotic adjustment, and lipid peroxidation of thymus were investigated in a two-year study from 2014 to 2015. Treatments consisted of 0, 200, and 400(iL L'1 chitosan applied to plants grown under field capacity, mild drought stress(50% field capacity), and severe drought stress(25% field capacity). Dry matter yield decreased substantially as drought stress intensified. However, essential oil content increased under stress conditions,with the highest essential oil yield obtained from plants under mild drought stress. Foliar application of chitosan compensated to some extent for dry matter and oil yield reduction of plants grown under drought stress. The highest essential oil yield(1.52 g plant-1) was obtained by application of 400 \iL L_1 chitosan under the mild stress condition in 2015 when plants were mature. The compensatory effect of chitosan in reducing the negative impact of stress conditions on dry matter and oil yield was due mainly to stimulation of osmotic adjustment through proline accumulation and reduction of lipid peroxidase level, which increased the integrity of cell membranes of thyme leaves.

关键词： thymus daenensis Chitosan Drought stress Essential oil yield Osmotic adjustment

X-ray irradiation selectively kills thymocytes of different stages and impairs the maturation of donor-derived CD4^+CD8^+ thymocytes in recipient thymus

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The Journal of Biomedical Research 2012年第5期26卷 355-364页

作者： Jinbo Li Hongquan Cai Jianliang Jin Qian Wang Dengshun Miao The Research Center for Bone and Stem Cells Department of Human Anatomy Nanjing Medical University Nanjing Jiangsu 210029 China Department of Preventive Medieine School of Public Health Nanjing Medical University Nanjing Jiangsu 210029 China

The aim of the present study was to determine whether the sensitivity of thymocytes to X-ray radiation depends on their proliferative states and whether radiation impairs the maturation of donor-derived thymocytes in recipient thymus.We assigned 8-week-old C57BL/6J mice into three treatment groups：1） untreated;2） X-ray radiation;3） X-ray radiation plus bone marrow transplantation with donor bone marrow cells from transgenic mice express-ing enhanced green fluorescent protein（GFP） on a universal promoter.After 4 weeks,the size of the thymus,the number and proliferation of thymocytes and ratios of different stage thymocytes were analyzed by immunohisto-chemistry and flow cytometry.The results showed that：1） CD4＋CD8＋ thymocytes were more sensitive to X-ray radiation-induced cell death than other thymocytes;2） the proliferative capacity of CD4＋CD8＋ thymocytes was higher than that of other thymocytes;3） the size of the thymus,the number of thymocytes and ratios of thymo-cytes of different stages in irradiated mice recovered to the normal level of untreated mice by bone marrow trans-plantation;4） the ratio of GFP-positive CD4＋CD8＋ thymocytes increased significantly,whereas the ratio of GFP-positive CD4＋ or CD8＋ thymocytes decreased significantly.These results indicate that the degree of sensitivity of thymocytes to X-ray radiation depends on their proliferative states and radiation impairs the maturation of donor-derived CD4＋CD8＋ thymocytes in recipient thymus.

关键词： thymus radiation CD4^＋CD8^＋ thymocytes sensitivity donor cells

Study of total phenol,flavonoids contents and phytochemical screening of various leaves crude extracts of locally grown thymus vulgaris

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Asian Pacific Journal of Tropical Biomedicine 2013年第9期3卷 705-710页

作者： Mohammad Amzad Hossain Khulood Ahmed Salim AL-Raqmi Zawan Hamood AL-Mijizy Afaf Mohammed Weli Qasim Al-Riyami School of Pharmacy College of Pharmacy and NursingUniversity of Nizwa

Objective:To prepare various crude extracts using different polarities of solvent and to quantitatively evaluate their total phenol,flavonoids contents and phytochemical screening of thymus vulgaris collected from Al Jabal Al Akhdar,Nizwa.Sultanate of Oman.Methods:The leave sample was extracted with methanol and evaporated.Then it was defatted with water and extracted with different polarities organic solvents with increasing polarities.The prepare hexane,chloroform,ethyl acetate,hutanol and methanol crude extracts were used for their evaluation of total phenol,flavonoids contents and phytochemical screening study.The established conventional metliods were used for quantitative determination of total phenol,flavonoids contents and phytochemical screening.Results:Phytochemical screening for various crude extracts were tested and shown positive result for flavonoids,saponins and steroids compounds.The result for total phenol content was the highest in hutanol and the lowest in methanol crude extract whereas the total flavonoids contents was the highest in methanol and the lowest hexane crude extract.Conclusions:The crude extracts from locally grown thymus vulgaris showed high concentration of flavonoids and it could be used as antibiotics for different curable and uncurable diseases.

关键词： thymus vulgaris L., Various crude extracts Phytochemical screening Soxhlet extractor Total phenol Flavonoids contents.

Antimycobacterial natural products from Moroccan medicinal plants: Chemical composition, bacteriostatic and bactericidal profile of thymus satureioides and Mentha pulegium essential oils

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Asian Pacific Journal of Tropical Biomedicine 2016年第10期6卷 836-840页

作者： Marwa Chraibi Abdellah Farah Sara Lebrazi Oumaima El Amine Mohammed Iraqui Houssaini Kawtar Fikri-Benbrahim Laboratory of Microbial Biotechnology Faculty of Science and Technology Sass Sidi Mohamed Ben Abdellah University Laboratory of Aromatic Plants Medicinal and Natural Substances National Institute of Medicinal and Aromatic PlantsPhytochemistry Department

Objective: To evaluate the susceptibility of Mycobacterium aurum and Mycobacterium smegmatis in vitro to the essential oils obtained from two medicinal plants: thymus satureioides(T. satureioides) and Mentha pulegium(M. pulegium), and to study their chemical composition.Methods: The aerial parts of T. satureioides and M. pulegium(leaves and stems) were hydro-distillated using a Clevenger-type apparatus and essential oils were analyzed and identified by gas chromatography-mass spectrometry. Antimycobacterial screening of essential oils was performed on the basis of the inhibition zone diameter by disc diffusion method against two mycobacterial strains whereas the minimal inhibitory concentration and minimal bactericidal concentration were determined by using the micro-dilution method.Results: Chemical analysis of their aerial part's essential oils gave as major compounds,borneol(34.26%), carvacrol(31.21%) and thymol(3.71%) for T. satureioides and R(+)-pulegone(75.48%), carvone(6.66%) and dihydrocarvone(4.64%) for M. pulegium.Thereafter their antimycobacterial effect evaluation, using the micro-dilution method,indicated that minimal inhibitory concentration values of T. satureioides essential oil ranged from 0.062% to 0.015%(v/v) and from 0.125% to 0.031%(v/v) for M. pulegium respectively against Mycobacterium aurum and Mycobacterium smegmatis.Conclusions: It is clearly evident from the results obtained that the Moroccan medicinal plants have great potential to be used as anti-tuberculosis agents. These findings may help scientists to undertake several research projects to discover useful natural product as new anti-tuberculosis drug.

关键词： thymus satureioides Mentha pulegium Essential oil Chemical composition Antimycobacterial activity

Gas chromatography-mass spectrometry analysis of different organic crude extracts from the local medicinal plant of thymus vulgaris L

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Asian Pacific Journal of Tropical Biomedicine 2013年第1期3卷 69-73页

作者： Laila Salim Al Hashmi Mohammad Amzad Hossain Afaf Mohammed Weli Qasim Al-Riyami Jamal Nasser Al-Sabahi School of Pharmacy College of Pharmacy and NursingUniversity of NizwaP.0.Box 33Postal Code 616NizwaSultanate of Oman Central Instrument Laboratory College of Agriculture and Marine SciencesSultan Qaboos UniversitySultanate of Oman

Objective:To isolate and analyze the chemical composition in different crude extracts of from the leaves of locally grown of thymus vulgaris L(T.vulgaris)by gas chromatography-mass spectrometry(GC-MS).Methods:The shade dried leaves powder was extracted with methanol by using Soxhlet extractor.Methanol crude extracts of T.vulgaris and the derived fractions of hexane,chloroform,ethyl acetate and butanol were obtained.Results:Qualitative analyses of various organic crude extracts of T.vulgaris by using GC-MS showed that there were different types of high and low molecular weight compounds.Most of the isolated and identified compounds by GC-MS in the crude extracts are basically biologically important.Further,the T.vulgaris leaf possessed certain characteristics that can be ascribed to cultivation on a domestic plantation.The crude extracts were prepared from the powder leaves of T.vulgaris for respective compounds can be chosen on the basis of above GC-MS analysis.Conclusions:All the major compounds were identified and characterized by spectroscopic method in different organic crude extracts of T.vulgaris are biologically active molecules.Thus the identification of a good number of compounds in various crude extracts of T.vulgaris might have some ecological role.

关键词： thymus vulgaris L Preparation crude extracts Soxhlet extractor GC-MS analyses

RUTHENIUM　COORDINATION　COMPOUNDS　WITH　POLYPYRIDYL：Ⅱ　THE　INTERACTION　OF　RUTHENIUM（Ⅱ）MIXED－LIGAND　COORDINATION　COMPOUNDS　CONTAINING　PYRIDO［3，2－f］［1，7］PHENANTHROLINE　WITH　CALF　thymus　DNA

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Chinese Chemical Letters 1995年第4期6卷 353-356页

作者： Lei WANG Jian Ying ZHOU Jin Ying WANG Tian Xian ZEMG Liang Nian JI(Department of Chemistry. Zhongshan University, Guangzhou. 510275)(State Key Labofatory of Coordination Chemistry, Manjing University . 210008) Zhongshan Univ Dept Chem Guangzhou 510275 Peoples R China Nanjing Univ State Key Lab Coordinat Chem Nanjing 210008 Peoples R China

The binding of Rulpdphen2+ to calf thymus DNA was inveatigated using absorption fluorescence and CD spectroscopies, where pdphen is the planar ligand pyrido[3. 2- f] [1, 7] phenanthroline,L=2, 2' -bipyridine, phenanthroline. 2, 9-dimethyl phenanthroline and 5-nitro-phenanthroline. All coordination componds show abaorption typochromicity associated with binding to calf thymus DNA .Ru(bipy) pdphen3+ and Rn (phen) pdphen+ also show fluoreacence intensities and excited state lifetime Increase becanse of the interaction of coordination compounds with calf thymus DNA .CD apectra of dialylatea indicate enantioselectivity associated withe binding to DNA .

关键词： DNA thymus COMPOUNDS CALF

Induction of immune tolerance with heart-thymus composite allotransplantation in rats

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Journal of Central South University of Technology 2005年第Z1期12卷 331-336页

作者： XIONG Hai-bo XIA Sui-sheng WEN Hao HUANG Zu-fa YE Qi-fa Medical Academy of Transplantation the Third Xiangya Hospital Central South University Changsha 410013 China Institute of Transplantation Tongji Hospital Huazhong Science and Technological University Wuhan 430030 China Department of General Surgery the First Affiliated Hospital Xinjiang Medical University Urumqi 830054 China Medical Academy of Transplantation the Third Xiangya Hospital Central South University Changsha 410013 China Medical Academy of Transplantation the Third Xiangya Hospital Central South University Changsha 410013 China

Objective To study on the role of thymus transplantation for heart allograft in rats. Methods Vascularized heart-thymus combined transplantation was performed with microsurgical technique. Graft survival, histopathology, level of IL-2, IL-4 and its mRNA expression in serum and cardiac grafts were investigated. Results Heart-thymus combined transplantation achieved effect in the prolongation of cardiac graft survival with short-term administration of cyclosporine. Conclusions Vascularized thymus transplantation induced immune tolerance in thymectomized rats.

关键词： thymus transplantation immune tolerance rejection rat