检索结果-南通市图书馆

Apoptotic and autophagic death union by thuja occidentalis homeopathic drug in cervical cancer cells with thujone as the bioactive principle

引用

Journal of Integrative Medicine 2022年第5期20卷 463-472页

作者： Asmita Pal Sucharita Das Soumalee Basu Rita Kundu Department of Botany University of CalcuttaKolkataWest Bengal 700019India Department of Microbiology University of CalcuttaKolkataWest Bengal 700019India

Objective‘Multi-targeting’drugs can prove fruitful to combat drug-resistance of multifactorial disease—cervical cancer.This study envisioned to reveal if thuja homeopathic mother tincture(MT)and its bioactive component could combat human papillomavirus(HPV)-16-infected SiHa cervical cancer cells since it is globally acclaimed for HPV-mediated warts.Methods thuja MT was studied for its antiproliferative and antimigratory properties in SiHa cells followed by microscopic determination of reactive oxygen species(ROS)generation by 2’,7’-dichlorodihydrofluorescein diacetate(DCFDA)staining and loss in mitochondrial membrane potential(MMP)by rhodamine(Rh)123 staining.Apoptosis and autophagy inductions were studied by acridine orange(AO)ethidium bromide(EB)staining and immunoblot analyses of marker proteins.The bioactive component of thuja MT detected by gas chromatography-mass spectrometry was studied for antiproliferative and antimigratory properties along with in silico prediction of its cellular targets by molecular docking and oral drug forming competency.Results thuja MT showed significant antiproliferative and antimigratory potential in SiHa cells at a 50%inhibitory concentration(IC50)dosage of 17.3μL/mL.An increase in DCFDA fluorescence and loss in Rh123 fluorescence prove that thuja MT acted through the burst of ROS and loss in MMP respectively.AO/EB-stained cells under the microscope and immunoblot analyses supported thuja-induced cellular demise via dual pathways—apoptosis and autophagy.Immunoblots showed cleavage of caspase-3 and poly adenosine diphosphate-ribose polymerase-1(PARP-1)along with upregulation of Beclin-1,microtubule-associated protein 1 light chain 3B(LC3B)-II,and p62 proteins.Hence,the apoptotic cascade followed a caspase-3-dependent pathway supported by PARP-1 cleavage,while autophagic death was Beclin-1-dependent and mediated by accumulation of LC3BII and p62 proteins.Thujone,detected as the bioactive principle of thuja MT showed greater anti-proliferative and anti-migratory potential at an IC

关键词： thuja Homeopathy Thujone Cervical cancer Apoptosis-autophagy crosstalk

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Phylogeny and Biogeography of thuja L. （Cupressaceae）, an Eastern Asian and North American Disjunct Genus

引用

Journal of Integrative Plant Biology 2005年第6期47卷 651-659页

作者： Jian-HuaLI Qiao-PingXIANG ArnoldArboretumofHarvardUniversity CambridgeMA02138USA LaboratoryofSystematicandEvolutionaryBotany InstituteofBotanytheChineseAcademyofSciencesBeijing100093China

In order to develop better insights into biogeographic patterns of eastern Asian and North American disjunct plant genera, sequences of nuclear ribosomal DNA internal transcribed spacer (nr DNA ITS) region were used to estimate interspecific relationships of thuja L. (Cupressaceae) and infer its biogeography based on the phylogeny. According to the phylogenetic analysis, two clades were recognized. The first clade included thuja plicata D. Don (western North America) and T. koraiensis Nakai (northeastern Asia), and the second one contained T. occidentalis(Gord.) Carr. (Japan). The ancestral area of thuja was inferred to be eastern Asia, and two dispersal events were responsible for the modern distribution of thuja in North America. Both the North Atlantic land bridge and Bering land bridge were possible routes for the migration of ancestral populations to North America.

关键词： biogeography Cupressaceae thuja nuclear ribosomal DNA internal transcribed spacer phylogeny

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Terpene Composition of Three Species of Gymnosperms from Vietnam

引用

American Journal of Plant Sciences 2013年第10期4卷 2031-2038页

作者： Do N. Dai Tran D. Thang Isiaka A. Ogunwande Faculty of Biology Vinh University Vinh City Vietnam Faculty of Chemistry Vinh University Vinh City Vietnam Natural Products Research Unit Department of Chemistry Faculty of Science Lagos State University Lagos Nigeria.

In the present investigation, we studied new essential oil contents and compositions of three individual plants from Cupressaceae family cultivated in Vietnam. The air-dried plants were hydrodistilled and the oils analysed by Gas chromatography (GC) and Gas chromatography couples with Mass spectrometry (GC-MS). The components were identified by comparison of their retention indices on HP-5 MS capillary column with literature reference and MS pattern with authentic library’s collection. a-Pinene (36.0%), α-cedrol (18.3%) and thujopsene (5.7%) were the main constituents of Cupressus tonkiensis Silba. Monoterpenes were the quantitative significant compounds of Sabina chinensis (L.) Antoine. These are bornyl acetate (19.5%), sabinene (17.3%) and a-pinene (15.8%). Moreover, the main compounds of thuja orientalis L., were identified as a-pinene (34.1%), α-cedrol (16.5%), β-caryophyllene (5.4%) and β-selinene (5.2%). The chemotaxonomy implication of these results was also discussed.

关键词： Cupressus tonkiensis Sabina chinensis thuja orientalis Bornyl Acetate α-Cedrol α-Pinene Sabinene

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：