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American Journal of Analytical Chemistry 2017年第1期8卷 1-18页

作者： Stephen Childs Nicolas Haroune Lee Williams Michael Gronow Department of Pharmacy Health & Well-Being Faculty of Applied Sciences University of Sunderland Sciences Complex Sunderland UK Cambridge Cancer Research Fund Cambridge UK

The low molecular weight thiols present in the deproteinized extract of a prostate cancer cell line (LNCaP-FGC) were analysed after derivatization with the Ellman reagent (ESSE). The mixed disulphides formed (RSSE) were fractionated, characterized and quantified by liquid chromatography on a C-18 column using UV detection. This revealed the presence, in femtomoles per cell, of glutathione (8.30 ± 0.73), cysteine (2.71 ± 0.04) and cysteinylglycine (0.83 ± 0.10), accounting for the bulk of the thiol present. Further analysis of the cell extracts using a novel and sensitive mass spectrometry technique allowed the detection of low level of an additional derivative which was identified as cysteinylglycerate using NMRspectroscopy.

关键词： Prostate Cancer Low Molecular Weight thiols thiol Analysis Ultra-High Performance Liquid Chromatography Novel thiol LC-MS NMR Ellman’s Reagent

Synthesis and Properties of Organic-Inorganic Hybrid Porous Polymers Obtained with Click Addition Reactions of thiol-Functionalized Random Type Silsesquioxane by and Diacrylate or Diisocyanate Compounds

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Open Journal of Polymer Chemistry 2020年第1期10卷 1-20页

作者： Tomoharu Miyanaga Naofumi Naga Graduate School of Engineering and Science Shibaura Institute of Technology Tokyo Japan Department of Applied Chemistry College of Engineering Shibaura Institute of Technology Tokyo Japan

Organic-inorganic hybrid network polymers have been synthesized by addition reaction of a thiol-functionalized random type silsesquioxane (SQ109) and alkyl diacrylate or diisocyanate compounds. Thiol-ene reaction of SQ109 and 1,4-butanediol diacrylate (BDA) successfully yield porous polymer in toluene initiated by azobis(isobutyronitrile) (AIBN) at 60°C. Morphology of the porous polymers was composed by connected globules, and the diameter of the globules decreased with increasing in the monomer concentration of the reaction system. By contrast, the reaction with 1,6-hexanediol diacrylate or 1,5-hexadiene yielded homogeneous clear gels. Thermal analyses of SQ109-BDA porous polymers indicated that thermal degradation of ester groups of BDA in the polymer network occurred at around 300°C. The porous polymer was also obtained by the reaction using a photo-initiator (Irugacure184) at room temperature, and showed higher Young’s modulus than the corresponding porous polymer obtained with the reaction with AIBN due to the small size of the globules. Young’s modulus

关键词： Silsesquioxane thiol Diacrylate Diisocyanate Gel Porous Polymer Morphology

Structure and interactions in α-crystallin probed through thiol group reactivity

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Advances in Biological Chemistry 2013年第5期3卷 427-439页

作者： Sudipa Saha Kali Pada Das Department of Biotechnology St. Xavier’s College Mother Teresa Sarani Kolkata India Department of Chemistry Bose Institute Kolkata India

a-Crystallin is the major structural protein of eye lens of vertebrates. In human lens, the ratio of aA-crystallin to aB-crystallin was found to be 3:1. aA-Crystallin contains two cysteine residues at positions 131 and 142, which are at the junction between the a-crystallin domain and the C-terminal tail. We used the accessibility of the thiol groups by Ellman’s reagent (DTNB) as a tool to gain information about the various structural perturbations of hinge region of a-crystallin and during the binding with substrates. In the native condition, the cys-142 though reacted quite fast was not fully exposed. Several reagents were used to see the accessibility of cys-131. Rate constant for cys-131 was increased gradually with increase in the concentration of reagents. The bindings of substrates are affected by the accessibility of thiol indicating that the substrates bind to the hinge region of a-crystallin. By blocking of cys-142, it was observed that the accessibility of one thiol depends on the other thiol, and they are not independent. The hinge region of a-crystallin is very important as substrate binding site and from this study we have got various structural information about that region.

关键词： thiol Reactivity α-Crystallin DTNB Kinetics Molecular Chaperone Folding Unfolding of α-Crystallin

REMOVAL OF thiol BY ACTIVATED CARBON FIBER

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Chinese Journal of Reactive Polymers 2000年第2期9卷 191-194页

作者： XU Zhida ZENG Hanmin FENG Yangqiao Materials Science Institute ofZhongshan University Guangzhou 510275China Research Institute ofGuangzhou Petrochemical Complex Guangzhou 510726China

Pitch based activated carbon fiber (PACF) was prepared, and influences of preparing condition such as activated temperature and time on the yield, specific surface area of PACF and its adsorption behavior towards n-butanethiol were studied. The results showed that simple PACF could not remove n-butanethiol from n-hexane solution. Therefore, dispersion of suitable catalyst such as cobalt salt on PACF is needed. Cobalt salt dispersed PACF can remove n-butanethiol quickly.

关键词： Activated carbon fiber Adsorption thiol Sweetening\

One-component intramolecular thiol-yne approach to peptide stapling

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Science Foundation in China 2016年第2期24卷 25-25页

With the support by the National Natural Science Foundation of China,the research team led by *** Zigang(李子刚)at Peking University Shenzhen Graduate School developed a new methodology for unprotected peptide staplin... 详细信息

关键词： intramolecular thiol Peking helical targeting restricted intriguing inability analogs hydrocarbon

The Effect of Using 1-Dodecanethiol as a Processing Additive on the Performances of Polymer Solar Cells

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Chinese Physics Letters 2013年第10期30卷 207-210页

作者： YANG Shao-Peng WANG Tie-Ning SHI Jiang-Bo ZHANG Ye LI Xiao-Wei FU Guang-Sheng Hebei Key Laboratory of Optic-Electronic Information Materials College of Physics Science and TechnologyHebei UniversityBaoding 071002

We introduce 1-Dodecanethiol(DT)to poly(3-hexylthiophene)(P3HT)/[6,6]-phenyl-C61-butyric acid methyl ester based polymer solar cells as a processing *** the amount of DT is 1vol%,the device performance is best.A final power conversion efficiency of 3.1%is achieved,which is an improvement of more than 40%compared to the reference solar cell without *** investigate the causes of improvement of the PCE,UV-vis spectroscopy,external quantum efficiency(EQE)is measured and an AFM is *** enhanced photovoltaic performances are discussed in terms of optical properties and the film morphology.

关键词： Solar additive thiol

Two-thiols-regulated fabrication of plasmonic nanoparticles with co-enhanced Raman scattering and circular dichroism responses

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Rare Metals 2023年第11期42卷 3673-3681页

作者： Jia-Qi Chen Han-Bo Li Xi Lu Xiao-Yang Liu Wei Feng Pan Wang Jian-Qiang Zhao Zhen-Yu Zhu Guang-Chao Zheng Xiao-Chun Wu School of Mechanical Engineering Chengdu UniversityChengdu610000China CAS Key Laboratory of Standardization and Measurement for Nanotechnology CASNational Center for Nanoscience and TechnologyBeijing100190China University of the Chinese Academy of Sciences Beijing100049China Innovation Research Center of Chiral Drugs Institute for Advanced StudyChengdu UniversityChengdu610106China School of Physics and Microelectronics Key Laboratory of Material PhysicsMinistry of EducationZhengzhou UniversityZhengzhou450001China Institute of Quantum Materials and Physics Henan Academy of SciencesZhengzhou450046China

Plasmonicnanoparticles(PNPs)with stable nanogaps are important to achieve strong,uniform and quantitative gap-enhanced Raman scattering(GERS)*** PNPs with plasmonic circular dichroism(PCD)responses have been discovered to be suitable for applications in enantiomeric recognition,cancer therapy and activation of immune ***,two-thiolsmodulated growth was demonstrated to result in the acquisition of PNPs with synergistically enhanced GERS and PCD signals.4-Aminothiophenol(4-ATP)and cysteine(Cys)played the role of Raman reporter and chiral stimulus,*** a fixed 4-ATP concentration,the GERS signal of PNPs was significantly enhanced with the increase of the concentration of ***,at a fixed concentration of Cys,an increase in PCD response was observed by elevating the concentration of *** aforementioned molecules acted as morphology controllers,leading to the formation of helical *** is suggested that the giant GERS and PCD response were contributed by the‘‘hot spots''within the PNPs and more perfect helical *** research pointed out a novel synthetic guideline to obtain PNPs with multiple functionalities by incorporating multi-ligands into the growth stages.

关键词： Gap-enhanced Raman scattering(GERS) Chirality thiol Plasmonic nanoparticles

Target-activated and ratiometric photochromic probe for “double-check” detection of toxic thiols in live cells

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Science China Chemistry 2019年第9期62卷 1204-1212页

作者： Youxin Fu Xing Zhang Feng Cao Wenhui Wang Guangren Qian Junji Zhang xKey Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering Feringa Nobel Prize Scientist Joint Research Center School of Chemistry and Molecular Engineering East China University of Science & Technology Shanghai 200237 China SHU Center of Green Urban Mining & Industry Ecology School of Environmental and Chemical Engineering Shanghai University Shanghai 200444 China Australian Institute for Bioengineering and Nanotechnology The University of Queensland Brisbane 4072 Australia Institute for Molecules and Materials Radboud University Heyendaalseweg 135 Nijmegen 6525AJ the Netherlands

Photochromic molecules can achieve reversible isomerization upon alternate light irradiations, which offers a great opportunity to improve the precision of analytes detection and imaging in complicated biological environments. Previous reported photochromic probe exhibited only mono-color switching and an initially fluorescence-ON state that may cause high background signal and impose an adverse impact on the desired sensing precision. To overcome this set-back, we developed a novel photochromic probe with an analyte-activation mode for ratiometric sensing of toxic thiols in both real water samples and live cells. The dynamic dual-fluorescence signal is released only after the fast and selective cleavage of the 2,4-dinitrophenyl sulfonate by the targeted thiophenol derivatives. Consequently, a "double-check" with synchronized dual-fluorescence blinking for analyte detection is successfully employed upon alternate light triggers with rapid response(k=7.2×10^(-2) s^(-1)), high sensitivity(LOD=6.1 nM) as well as selectivity of thiophenol derivatives over other common thiol species(e.g., GSH, Cys and Hcy). The photochromic probe was successfully introduced to the fast and on-site detection of highly toxic thiophenols in real waste water samples. Moreover, by using confocal laser-scanning microscopy(CLSM) and flow cytometric analysis, the potential applications of this ratiometric photochromic probe for trace toxic thiol sensing in live cells are examined.

关键词： photochromism ratiometric probe live cell imaging target-activation thiol sensing

Chemical interactions between silver nanoparticles and thiols: a comparison of mercaptohexanol against cysteine

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Science China Chemistry 2014年第9期57卷 1199-1210页

作者： TOH Her Shuang BATCHELOR-MCAULEY Christopher TSCHULIK Kristina COMPTON Richard G Department of Chemistry Physical and Theoretical Chemistry LaboratoryUniversity of OxfordSouth Parks RoadOxford OX1 3QZUK

The interaction between citrate capped silver nanoparticles and two different thiols, mercaptohexanol （MH） and cysteine, was investigated. The thiols interacted with silver nanoparticles in a significantly contrasting manner. With MH, a sparingly soluble silver（1） thiolate complex AgSRm （Rm = -（CH2）6OH） was formed on the silver nanoparticle surface. Cyclic voltammograms and UV-vis spectra were used to infer that the AgSRm complex on the nanoparticle surface undergoes a phase transition to give a mixture of AgSRm and Ag2S-like complexes. In contrast, when silver nanoparticles were exposed to cysteine, the citrate cap- ping agent on the silver nanoparticles was replaced by cysteine to give cysteine capped nanoparticles. As cysteine capped nanoparticles form, the electrochemical data displayed a decrease in oxidative peak charge but the UV-vis spectra showed a constant signal. Therefore, cysteine capped nanoparticles were suggested to have either inactivated the silver surface or else pro- moted detachment from the electrode surface.

关键词： silver nanoparticles interaction thiol

Small molecule fluorescent probes of protein vicinal dithiols

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Chinese Chemical Letters 2019年第10期30卷 1704-1716页

作者： Guodong Hu Huiyi Jia Lanning Zhao Dong-Hyung Cho Jianguo Fang State Key Laboratory of Applied Organic Chemistry College of Chemistry and Chemical EngineeringLanzhou UniversityLanzhou 730000China School of Life Sciences Kyungpook National UniversityDaegu 41566Republic of Korea

The vicinal dithiol motif is widely present in proteins, and is critical for proteins’ structures and *** recent years, a variety of fluorescent probes with high specificity and outstanding optical properties for sensing protein vicinal dithiols have been developed. In this review, we summarized the fluorescent probes of protein vicinal dithiols in literature. These probes are classified into four types based on their acceptor sites, i.e., biarsenical probes, monoarsenical probes, dimaleimide probes and diacrylate *** analyzing the properties of different probes, we expect that this review would help readers further understand the structural factors of these probes and provide the design strategy for novel fluorescent probes with improved properties.

关键词： Fluorescent probe Protein vicinal dithiols Arsenical probe thiol redox Reaction-based probe