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Reduced thiol Compounds-Induced Biosensing,Bioimaging Analysis and Targeted Delivery

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Chinese Journal of Chemistry 2020年第12期38卷 1793-1806页

作者： Yingshu Guo Xiaofei Zheng Xiuping Cao Wenxin Li Di Wu Shusheng Zhang Collaborative Innovation Center of Tumor Marker Detection Technology Equipment and Diagnosis-Therapy Integration in Universities of ShandongShandong Province Key Laboratory of Detection Technology for Tumor MarkersSchool of Chemistry and Chemical EngineeringLinyi UniversityLinyiShandong 276005China

Reduced thiol compounds(RTCs),such as glutathione(GSH),cysteine(Cys),and homocysteine(Hcy),are important components of many amino acids *** are also basic buiding blocks that make up organisms and play an important role in physiology and ***,based on thefundamental characteristics of RTCs in vivo,researchers have reported their use in biosensing and imaging analysis and as carriers inlfe sciences andhealth sciences.n this review,we will itroduce the principles of RTLs and highlight their applications in biosensing and imaging analysis and as carriersreported from 2015 to 2020.

关键词： thiol principles fundamental

A New Pyrene-Spacer-Maleimide Dyad for Sensing Molecules with One or Two thiol Groups

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Chinese Journal of Chemistry 2006年第9期24卷 1122-1125页

作者：王卓张德清朱道本 Beijing National Laboratory for Molecular Sciences Organic Solids Laboratory Institute of Chemistry Chinese Academy of Sciences Beijing 100080 China Graduate School of Chinese Academy of Sciences Beijing 100080 China

A new pyrene-spacer-maleimide dyad 1 was used to selectively detect cysteine in the presence of other amino acids, and sequentially react with dithiols to generate the molecule with two pyrene units showing typical ex... 详细信息

关键词： pyrene excimer maleimide thiol

ROLE OF thiol-DISULFIDE EXCHANGE IN INSULIN BINDING TO ITS RECEPTOR

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Science China Chemistry 1992年第4期35卷 429-436页

作者：谢贵福李淑莲王志珍 Institute of Biophysics Academia Sinica Beijing 100080 PRC

The effects of reduction by DTT, oxidation by DTNB and treatment with NEM on the thiol contents and insulin binding to its receptor in mice liver membranes were studied. Reduction with DTT leads to a parallel increase in the thiol content and the speciflc binding of insulin to the membrane. Scatchard analysis of the results shows little change in the number of binding sites but a twofold increase of the binding constant. Washing the membrane with bound insulin by a DTT containing buffer results in a more marked increase in the release of bound insulin than washing with buffer alone, suggesting that part of the insulin is bound to its receptor by covalent disulfide linkages through a thiol-disulfide exchange reaction and reduction with DTT leads to a marked increase in this 'disulfide-linked' insulin. Treatment with DTNB or NEM of the DTT-reduced membrane seems to reverse the effect of DTT reduction, although the reaction of the untreated membrane with DTNB or NEM had little or no effect on the specific binding of insulin. It is suggested that initially, part of the thiols responsible for the exchange reaction may not be available for reaction with DTNB and reduction with DTT generates further thiols leading to increased specific binding in general and increased insulin binding to the receptor through covalent disulfide linkages in particular.

关键词： thiol disulfide insulin receptor membrane.

thiol-free route to diaryl sulfides by Cu catalyzed coupling of sodium thiosulfate with aryl halides

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Chinese Journal of Catalysis 2016年第9期37卷 1550-1554页

作者： najmeh nowrouzi mohammad abbasi hadis latifi Department of Chemistry Faculty of Sciences Persian Gulf University Bushehr 75169 Iran

A method for the synthesis of diaryl sulfides from aryl halides in polyethylene glycol was *** Na2S2O3·5H2O,which is readily available as a stable salt,is an effective source of sulfur in the presence of Cu I as cata... 详细信息

关键词： Copper catalyst Sulfide thiol Sodium thiosulfate

The Synthesis of Neodymium Complexes with Polymers Containing thiol and Sulphoxide and Their Catalytic Activities

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Chemical Research in Chinese Universities 1993年第4期9卷 364-366页

作者： QU Ya-huan and LI Xiao-li (Department of Chemistry, Changchun Normal College, Changchun, 130032)YU Guang-qian and LI Yu-liang (Changchun Institute of Applied Chemistry, Academia Sinica, Changchun, 130022)

Introduction Polymers containing thiol and sulphoxide which have active—SH and—SO groups respectively, have wide application to biological, pharmaceutical, industrial and other chemical field and—SH and—SO groups are easy to form complexes with metals, so they have aroused an increasing interest of many authors. A large number of functional resins and corresponding metal complexes have been synthesized.

关键词： Neodymium complexes Polymers thiol Sulphoxide Catalytic activities

thiol-Disulphide Balance:Could Be a New Marker for Thyroid Cancer?

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Journal of Cancer Therapy 2018年第8期9卷 598-604页

作者： Ayse Ozdemir Ozcan Erel Department of Biochemistry Usak Training and Research HospitalUsak UniversityUsakTurkey Department of Biochemistry Ankara Atatürk Training and Research HospitalYildirim Beyazit UniversityAnkaraTurkey

Objectives. Cancer is a very widespread disorder known in world wide since long, but its biochemical features remain unclear. Thyroid carcinomas are the most common endocrine cancer and its frequency continues to escalate. There is evidence that the serum concentration of TSH is an unreliant predictor for the diagnosis of thyroid cancer. The formation of the plasma thiol pool from low and large molecular weight proteins suggests that thiol/disulfide balance is important in cancerous cases. The aim of this study was to investigate an oxidative stress marker (thiol/disulphide homeostasis) and IMA (Ischemia modified albumin), Albumin, CEA (Carcinoembryonic antigen), TSH (Thyroid stimulate hormone), thyroxine (T4), free thyroxine (FT4), triiodothyronine (T3) and free triiodothyronine (FT3) in patients with thyroid cancer and compare the results with healthy controls for the first time in literature. Materials-Methods: A total of 43 participants including 23 patients with thyroid cancer and 20 healthy individuals were included in the study. Serum levels of TSH, T4, FT4, T3 and FT3 have been measured during treatment and follow-up of patients with thyroid carcinoma. Serum levels of TSH, T4, FT4, T3 and FT3, IMA, Albumin, CEA, Native thiol (-SH), disulfide (-S-S) and total thiol (TT) as well as disulphide/native thiol and disulphide/total thiol ratios were compared between the groups. Native thiol, disulfide and total thiol concentrations were measured with a novel automated method (Roche, cobas 501, Mannheim, Germany). Results and conclusion: This paper discusses an oxidative stress marker (thiol/disulphide homeostasis) and tumor markers IMA, Albumin, CEA, TSH, T4, FT4, T3 and FT3 in patients with thyroid cancer and compare the results with healthy controls. Mean age at participant was 41.73 years for thyroid cancer patients (21 females/2 males). A control group of 20 participants was included the study (19 females/1 male, mean age 51.75).

关键词： Disulphide thiol Tumor Marker Thyroid Cancer

Quantification of Global Protein Disulfides and thiol-Protein Mixed Disulfides to Study the Protein Dethiolation Mechanisms

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American Journal of Analytical Chemistry 2013年第10期4卷 9-19页

作者： Lucia Coppo Raffaella Priora Sonia Salzano Pietro Ghezzi Paolo Di Simplicio Brighton and Sussex Medical School Trafford Centre Falmer Brighton UK Department of Neuroscience Pharmacology Unit University of Siena Siena Italy

The redox state of cellular thiols is widely studied because it was recently linked to many different diseases and pathologies. In this work we quantified the concentrations of protein disulfides (PSSP) and thiol-protein mixed disulfides (XSSP) in rat tissues (liver, kidney and heart) and cells (Raw 264.7) by an improved method of XSSP and PSSP determination after oxidative stress induced by diamide. Under native and denaturing conditions, a thiol block by N-ethymaleimide was introduced to avoid thiol exchange reaction activations by protein SH groups (PSH) (PSH + XSSP ←→ PSSP + XSH) and alterations of original XSSP/PSSP levels. Low molecular weight thiols (XSH) and PSH were respectively measured by HPLC on supernatants and on corresponding pellets by DTNB (Ellman’s reagent) after dithiothreitol reduction. PSSP concentrations of liver, heart and kidney were respectively 0.304, 0.605 and 0.785 μmoles/g and after diamide exposure they were significantly augmented of about 65%-70% in liver and heart, but not in the kidney. Normal XSSP, that were -20 times lower than normal PSSP were induced by diamide in liver and heart of about 40 times, but not in kidney. Thermodynamic criteria regarding the pKa values of thiols engaged as PSSP and GSSP were used to interpret dethiolation mechanisms via thiol exchange reactions.

关键词： Protein Disulfides S-Glutathionylation thiol pKa Diamide Oxidative Stress

Studies on the thiol Components of Isolated Nuclei

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Journal of Analytical Sciences, Methods and Instrumentation 2020年第1期10卷 36-42页

作者： Michael Gronow Cambridge Cancer Research Fund Laboratory 7 The Maltings Cottenham Cambridge England UK

The thiol components of the nonhistone proteins prepared from isolated nuclei from rat liver, regenerating liver and hepatoma 223 cells have been investigated after reaction with radio labelled N-ethylmaleimide and 5-5’-dithiobis-(2-nitrobenzoic acid) (DTNB). The labelled adducts formed were examined by isoelectric focusing analysis in polyacrylamide gel and the distribution of the radiolabels within sliced portions of the gels determined. In the case of the 14C labelled NEM adduct the label was found to be spread amongst numerous protein components within the gel however, in the case of the 35S labelled DTNB adducts, only a small proportion of the label was found in the protein material which was retained in the acidic isoelectric point (pI) region of the gel. The bulk of the 35S labelled adduct (56% - 60%) was found to have migrated into the anode solution (10 mM phosphoric acid). This could be adsorbed onto a hydrophobic resin (XAD2) resin and eluted with methanol. Gel filtration chromatographic analysis of this adduct material on BioGel P2, (exclusion limit 1500 daltons) showed low molecular weight components to be present. Slightly different patterns were obtained for these nuclei, each containing several 35S components with molecular weights greater than the Ellman reagent itself. These 35S labelled thiol components did not contain any protein, peptide or amino acid components indicating strongly that a novel species of thiols could be present in these nuclei bound within the non-histone protein matrices.

关键词： thiols Isolated Cell Nuclei Radiolabelled thiol Adducts Isoelectric Focusing Analysis

Molecular Cloning of a thiol Proteinase Inhibitor Gene and Its Expression in ***

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High Technology Letters 1996年第2期2卷 89-94页

作者：周兆斓 Institute of Genetics Academia SinicaBeijing 100101P.R.China

A cDNA library was constructed with 1.5×10~6 pfu from rice immature seeds,fromwhich a cDNA clone for rice thiol proteinase inhibitor,oryzacystatin(OC),was isolated byscreening with synthesized oligodeoxynucleotide probe,which contained a 309bp open read-ing frame,84bp 5′-end noncoding region and a poly(A)signal AATAAA at the 3′-end fol-lowed by 31Nt poly(A).Then the coding region of OC was amplified and inserted into thedownstream of λP_RP_L promoter for thermal-inducible expression in *** the cul-ture temperature from 30℃ to 42℃ led to a high level expression of OC,which exhibited adistinct band of 12.0 kDa and accounted for at least 10% of the total soluble proteins *** papain-inhibitory activity of the expressed OC was further confirmed.

关键词： Rice cDNA library thiol proteinase inhibitor Insect-resistant gene Sequence analysis High level expression