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Preliminary Studies on Express Fermentation Process Enhancing thermophilic bacteria Proliferation and Quality of a Substrate for the Cultivation of Pleurotus ostreatus in Ukraine

Preliminary Studies on Express Fermentation Process Enhancin...

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第五届国际药用菌大会

作者： IRYNA BANDURA OMOANGHE S.ISIKHUEMHEN Ukrmiceliy 82a Slepnova StreetDonetsk-57Ukraine 83057 North Carolina Agricultural and Technical State University 1601 E.Market StreetGreensboro NC 27411 USA

＜正＞Rapid solid fermentation process for enhancing thermophilic bacteria proliferation in wheat straw based substrate for oyster mushroom cultivation was *** results indicated that fermentation time 48 hours resulted in substrate with highest mushroom yield（92%biological efficiency） and the least incidence of *** organisms,whose titer is increased when using this express method of substrate preparation,provides a reliable defense from mold fungi *** infestations of fruit bodies were not observed even once during the four years of application of this *** substrate pretreatment method which eliminates the need to use additional agents to control contaminants and the lowered energy cost for substrate pretreatment is a significant improvement in technology which guarantees return on investment and sustainability in small oyster mushroom farms in *** express fermentation method of substrate is superior to steam sterilization that is currently in use in most farms in Ukraine.

关键词： solid state fermentation thermophilic bacteria oyster mushrooms fruit body yield substrate pretreatment

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Isolation and molecular identification of cellulolytic bacteria from Dig Rostam hot spring and study of their cellulase activity

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BIOCELL 2020年第1期44卷 63-71页

作者： Sareh HAJIABADI Mansour MASHREGHI Ahmad Reza BAHRAMI Kiarash GHAZVINI Maryam M.MATIN Department of Biology Faculty of ScienceFerdowsi University of MashhadMashhadIran Novel Diagnostics and Therapeutics Research Group Institute of BiotechnologyFerdowsi University of MashhadMashhadIran Microbiology Research Center&Department of Microbiology and Virology Ghaem Medical CenterMashhad University of Medical SciencesMashhadIran

Cellulose is the main structural component of lignocellulosic wastes that can be converted to sugars and biofuels by *** to wide applications of this enzyme in various industries around the world,cellulase is considered as the third industrial *** ability of thermophilic bacteria in the production of heat-stable cellulases has made them valuable tools in *** aim of this study was isolation and molecular identification of cellulolytic thermophile bacteria from Dig Rostam hot spring and investigating their cellulase *** were taken from water and sediments of this hot spring,and cellulolytic bacteria were enriched in media containing cellulose as the only carbon *** bacteria were incubated at 60℃,and single colonies were then isolated on solid *** red assay was used as a quick test for the qualitative screening of cellulase *** to these qualitative results,four colonies named CDB1,CDB2,CDB3,and CDB4 were isolated,and their growth curve and some other characteristics were determined by biochemical ***,endoglucanase,exoglucanase,and FPase activities of the isolates were investigated *** indicated that CDB1 exhibited the highest endoglucanase(0.096 U/mL)and exoglucanase(0.156 U/mL)activities among other isolates.16S rDNA partial sequencing indicated that CDB1 had 99%similarity to the genus Anoxybacillus,and the other isolates showed the highest similarity to the genus *** cellulase gene of CDB1 isolate with the highest cellulase activity was also cloned,and its sequence is reported for the first *** studies on this thermophilic enzyme might be useful for industrial applications.

关键词： Anoxybacillus Cellulase Enzyme activity assay thermophilic bacteria 16S rDNA

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