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Wolbachia-induced expression of kenny gene in testes affects male fertility in Drosophila melanogaster

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Insect Science 2020年第5期27卷 869-882页

作者： John C.Biwot Hua-Bao Zhang Chen Liu Jun-Xue Qiao Xiao-Qiang Yu Yu-Feng Wang School of Life Sciences Hubei Key Laboratory of Genetic Regulation and Integrative BiologyCentral China Normal UniversityWuhanChina

Wolbachia are Gram-negative endosymbionts that are known to cause embry-onic lethality when infected male insects mate with uninfected females or with females carrying a different strain of Wolbachia,a situation characterized as cytoplasmice incom-patibility(CD).However,the mechanism of CI is not yet fully understood,although re-cent studies on Drosophila melanogaster have achieved great progress.Here,we found that Wolbachia infection caused changes in the expressions of several immunity-related genes,including significant upregulation of kenny(key),in the testes of D.melanogaster.Overexpression of key in fly testes led to a significant decrease in egg hatch rates when these fies mate with wild-type females.Wolbachia-infected females could rescue this embryonic lethality.Furthermore,in key overexpressing testes terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick-end labeling signal was significantly stronger than in the control testes,and the level of reactive oxygen species was significantly increased.Overexpression of key also resulted in alterations of some other immunity-related gene expressions,including the downregulation of Zn72D.Knockdown of Zn72D in fly testes also led to a significant decrease in egg hatch rates.These results suggest that Wolbachia might induce the defect in male host fertility by immunity-related pathways and thus cause an oxidative damage and cell death in male testes.

关键词： Drosophila melanogaster key testes Wolbachia Zn72D

Induction of Apoptosis in the Immature Mouse testes by a Mixture of Melamine and Cyanuric Acid

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Journal of Integrative Agriculture 2012年第12期11卷 2058-2066页

作者： YOU Hua ZHU Jin-feng SHE Rui-ping CHANG Ling-ling SHI Rui-han DING Ye CHI Li-juan LIU Bin YUE Zhuo TIAN Ji-jing MAO Jing-jing SU Li-fang Key Laboratory of Zoonosis Ministry of Agriculture/Department of Veterinary Pathology College of Veterinary Medicine China Agricultural University China Animal Disease Control Center National Animal Husbandry Service

The toxicity of melamine has attracted much attention since the recent outbreaks of renal injury in pets and infants. Previous studies indicated that melamine by itself had low toxicity, whereas a mixture of melamine and cyanuric acid （M＋CA） could cause serious renal damage. At present, most researches on the toxicity of M＋CA are focused on the kidney. However, little is known about the adverse effects of this mixture on the reproductive system. In the present study, the toxicity of M＋CA to testes was investigated. Immature male mice were orally dosed with 0, 0.6, 3, and 15 mg kg-1 d-1 of a 1：1 M＋CA for 28 d. Pathological changes occurred in germ cells, such as loose arrangement, reduced numbers and karyopyknosis, indicating that this mixture was toxic to spermatogenesis. Compared with the control group, the TUNEL- positive germ cells increased significantly and the ratio of Bcl-2 to Bax, total antioxidant capacity and superoxide dismutase activity decreased significantly in the 3 and 15 mg kg-1 d- M＋CA treated group, while the activities of caspase-3, caspase- 8 and caspase-9 remained unchanged. The results suggest that M＋CA can induce apoptosis in the mice testes. The downregulation of Bcl-2/Bax and oxidative stress may play a pivotal role in the induction of apoptosis by M＋CA in mice testes.

关键词： melamine/cyanuric acid mixture testes injury caspase-independent apoptosis

Alleviating effects of melatonin on oxidative changes in the testes and pituitary glands evoked by subacute chlorpyrifos administration in Wistar rats

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Asian Pacific Journal of Tropical Biomedicine 2012年第8期2卷 645-650页

作者： Angela J Umosen Suleiman F Ambali Joseph O Ayo Bisala Mohammed Chidiebere Uchendu Department of Veterinary Pharmacology Ahmadu Bello University Department of Veterinary Physiology Ahmadu Bello University Department of Veterinary Pathology Ahmadu Bello University

Objective:To evaluate the alleviating effects of melatonin on oxidative changes in the testes and pituitary gland induced by subacute chlopyrifos（CPF） exposure in rats.Methods:Forty adult male Wistar rats divided into 4 groups of 10 animals were used for the study.Croup I received soya oil（2 mL/kg） while group II was administered with melatonin（0.5 mg/kg）.Group III was administered CPF only(8.5 mg/kg l/10th of the LD50) while group Ⅳ was pretreated with melatonin（0.5 mg/kg） and then exposed to CPF（8.5 mg/kg）,10 min later.The regimens were administered by gavage once daily for a period of 28 d.At the end of the exposure period, the rats were sacrificed and the testicular tissues and pituitary glands were evaluated for the malonaldehyde（MDA） concentration and activities of superoxide dismutase（SOD） and catalase（CAT）.Results:CPF increased MDA concentrations and reduced the activities of SOD and CAT in the testes and pituitary gland.Melatonin pretreatment reduced the testicular and pituitary MDA concentrations and improves the SOD and CAT activities.Conclusions:the study showed that subacute CPF-induced oxidative stress in the testes and pituitary glands were alleviated by melatonin due to its antioxidant property.

关键词： Chlorpyrifos Oxidative stress testes Pituitary glands Alleviation Melatonin

Associations between testicular development and fetal size in the pig

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Journal of Animal Science and Biotechnology 2022年第5期13卷 1340-1350页

作者： Claire Stenhouse Yennifer Cortes-Araya F.Xavier Donadeu Cheryl J.Ashworth Functional Genetics and Development Division The Roslin Institute and Royal(Dick)School of Veterinary StudiesUniversity of EdinburghMidlothianUK Department of Animal Science Texas A&M University440 Kleberg CenterCollege StationTX 77843-2471USA

Background:Impaired reproductive performance is the largest contributing factor for the removal of boars from commercial systems.Intrauterine growth restricted piglets represent 25%of the total number of piglets born and have impaired reproductive performance.This study aimed to improve the understanding of temporal changes in testicular gene expression during testes development in fetuses of different size.The lightest and closest to mean litter weight(CTMLW)male Large White×Landrace littermates were collected at gestational days(GD)45,60 and 90(n=5-6 litters/GD).Results:testes weight and testes weight as a percentage of fetal weight were not associated with fetal size at GD60 or 90.Fetal plasma testosterone was not associated with fetal size at GD90.There was no association between fetal size and seminiferous tubule area and number,number of germ or Sertoli cells per tubule.The lightest fetuses tended to have wider seminiferous tubules compared to the CTMLW fetuses at GD90(P=0.077).The testicular expression of KI67(P≤0.01)and BAX:BCL2 ratio(P=0.058)mRNAs decreased as gestation progressed.Greater SPP1 mRNA expression was observed at GD60 when compared with GD45 and 90(P≤0.05).Lower expression of DMRT1 and SPP1(Ptestes associated with the lightest fetuses compared to the CTMLW fetuses at GD90.Conclusions:These findings provide novel insights into the expression profiles of genes associated with testicular development and function.Further,these data suggest that programming of reproductive potential in IUGR boars occurs late in gestation,providing a platform for further mechanistic investigation.

关键词： Fetal growth Intrauterine growth restriction(IUGR) Porcine Pregnancy testes

Effect of Combination Regimen of Low-dose Gossypol Acetic Acid with Steroid Hormones on Expression of Protein Kinase C alpha (PKC-α) and Cyclin D1 in Rat testes

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Journal of Reproduction and Contraception 2012年第4期23卷 199-208页

作者： Jin-hui SHAO Zeng-lu XU Xiao-jing QIAN Fang LIU Hui HUANG Department of Anatomy Histology and Embryology Institute of Basic Medical Sciences Chinese Academy of Medical Sciences School of Basic Medicine Peking Union Medical College Department of Histology and Embryology School of Medicine Hubei University of Art and Science

Objective To investigate the contraceptive mechanism of combination regimen of low- dose gossypol acetic acid （GA） with steroid hormones [desogestre/ethinylestradiol/ testosterone undeeanote（DSG/EE/TU）]. Methods Adult male rats were randomly divided into four groups. Group GH： rats were fed orally with gossypol acetic acid （GA, 12.5 mg/kg） and desogestrel （DSG, 0.125 mg/kg）/ ethinylestradiol （EE, 0.025 mg/kg）/testosterone undecanoate （TU, 100 mg/kg per day, qd X4 weeks or 10 weeks）; group G： a single dose of GA （12.5 mg/kg per day, qd X 4 weeks or I0 weeks）; group H： the same dosage of DSG/EE/TUas in group GH; group C： rats were treated with vehicle （1% methyl cellulose） as the control. Expression of protein kinase C alpha （PKC-a） and cyclin D1 in rat testes were tested mainly by immunohistochemistry （IHC） and Western blotting. Results IHC results showed that protein PKC- a was expressed mainly in interstitial tissue of testis among seminiferous tubule. The expression of PKC-oc in groups H and GH at week 10 was decreased greatly compared with that in group C. The protein cyclin D1 was expressed mainly in residual body of seminiferous tubule eavosurface and interstitial tissue among seminiferous tubule of testis. Western blotting results showed that the expression of PKC-a in groups H and GH at week 10 was decreased significantly compared with that in group C （P〈0.05）. The expression of cyclin D1 in groups G, H or GH at week 10 rose significantly compared with that in group C （P 〈0.05）.Conclusion The administration of low-dose gossypol acetic acid with steroid hormones for 10 weeks can decrease the expression of PKC-a greatly.

关键词： gossypol steroid hormones protein kinase C alpha （PKC-a） cyclin D 1 rat testes

Hard life in cold waters:Size distribution and gonads show that Greenland halibut temporarily inhabit the Siberian Arctic

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Water Biology and Security 2022年第2期1卷 96-104页

作者： A.N.Benzik L.K.Budanova A.M.Orlov Polar Branch of the Russian Federal Research Institute of Fisheries and Oceanography(PINRO Named after N.M.Knipovich) MurmanskRussia Shirshov Institute of Oceanology of the Russian Academy of Sciences(IO RAS) MoscowRussia Russian Federal Research Institute of Fisheries and Oceanography(VNIRO) MoscowRussia A.N.Severtsov Institute of Ecology and Evolution RAS(IPEE RAS) MoscowRussia Tomsk State University(TSU) TomskRussia Dagestan State University(DSU) MakhachkalaRussia Caspian Institute of Biological Resources Dagestan Federal Research Center of the Russian Academy of Sciences(CIBR DFRC RAS)MakhachkalaRussia

The range of the Greenland halibut Reinhardtius hippoglossoides(Walbaum,1792)includes vast areas in the northern parts of the Atlantic and Pacific oceans,as well as the seas of the Arctic Ocean.Despite its commercial importance and decades of study,many aspects of its life cycle and reproduction remain poorly understood.Here,we evaluate the size distribution of Greenland halibut in the catches of research surveys in the Barents,Kara,and Laptev seas and conduct micro-and macroscopic studies of their gonads in the Laptev Sea.The size of Greenland halibut individuals increases from west to east,which is associated with the settling of pelagic juveniles and the subsequent residency of growing individuals near their settling sites.To the greatest extent,this size imbalance is manifested in the areas most remote from spawning grounds,i.***.the Kara and Laptev seas.The process of maturation in large individuals of Greenland halibut in the Arctic seas is characterized by a state of inhibitionwaiting in the early stages of gametogenesis(previtellogenesis).The data obtained indicate that Greenland halibut in the North Atlantic and the Siberian Arctic have a continuous range.The continental slope of the Barents Sea is a spawning and maturing ground,while the northern parts of the Barents and Kara seas,as well as the continental slope of the Laptev Sea,are feeding grounds for juveniles.The results of this study might serve as a necessary basis for monitoring condition of halibut stocks as well as for reallocation of the total allowable catch between countries that exploited them in the Norwegian and Barents seas.

关键词： Reinhardtius hippoglossoides Lifecycle Reproduction testes Ovaries Spawning ground Maturation Barents Sea Kara Sea Laptev Sea

The Danger within:Covid-19 Affinity for ACE2 Receptors in Adipose Tissue and testes.The Protective Effects of Estradiol,Fitness,and Weight Management

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Journal of Endocrinology Research 2020年第1期2卷 1-14页

作者： Xanya Sofra City University LondonNew School for Social ResearchNew YorkUnited States

The imminent danger of the Covid-19 pandemic has accelerated research in pharmaceuticals that either target the viral Spike proteins fusion with ACE2 receptors,or the infectious RNA replication that often overwhelms immune defences.The scope of this review was to elucidate the main human vulnerabilities to Covid-19,including the accumulation of ACE2 receptors in testes,adipose tissue,thyroid,heart and kidneys that escalate viral affinity in males,the aged,and certain medical conditions,including diabetes,CVD,and pulmonary diseases.Pre-existing inflammation inherent in obesity may exacerbate the“cytokine storm,”a rampaging immune reaction during the course of Covid-19 that is deleterious to the host.We examined the molecular dynamics illustrating the action of new therapeutics necessary for Covid-19 patients;the estradiol advantage hypothesis;alternative therapies including hormone replacement procedures and mesenchymal stem cells;plus preventive and protective interventions.The current perspective also explored the primary components of dysregulated health predisposing individuals to Covid-19,including hormonal imbalance,increased lipids and lipoproteins,thyroid dysfunction,degraded fitness,and age-related testosterone decline accompanied by cortisol increase that provokes stress eating behaviours and weight accumulation.Obesity increases the probability of Covid-19 infection due to its abundance of ACE2 receptors;while physical activity may decrease Covid-19 vulnerability,by reducing fat and increasing muscle mass that manifests a relatively inhibited ACE2 expression.Several weight management solutions feature lasers and radiofrequency which diminish subcutaneous adiposity but do not enhance fitness.A data metanalysis of seven recently published clinical studies on 95 obese individuals,73 males and 22 females with an average BMI of 30.9,demonstrated visceral fat reduction combined with increased skeletal muscle mass.It also revealed a statistically significant decrease in BMI,lipids,lipoproteins,inflammation and

关键词： Visceral Adipose Tissue ACE2 testes Covid-19 Estradiol Fitness Weight management Leptin Free T3 Testosterone BMI BMR VLDL Triglycerides HDL Creatinine Bilirubin Ghrelin Insulin IGF-1 Cortisol CRP Diabetes Prediabetes Hyperphagia

Histomorphometry of the testes, Epididymides, and Spermatozoa of Four Indigenous Breeds of Bulls in Ibadan, Nigeria

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Open Journal of Veterinary Medicine 2021年第9期11卷 299-306页

作者： Ayodeji Ayotunde Oni Matthew Olugbenga Oyeyemi Joseph Atawalna School of Veterinary Medicine College of Basic and Applied Sciences University of Ghana Legon-Accra Ghana Department of Theriogenology Faculty of Veterinary Medicine University of Ibadan Ibadan Nigeria School of Veterinary Medicine Kwame Nkrumah University of Science and Technology Kumasi Ghana

Forty testicles were used to carry out histomorphometry study on the testes, epididymides and spermatozoa of four indigenous breeds of bull found in Ibadan (Red Bororo (RB), Sokoto Gudali (SG), White Fulani (WF) and Dhali (DL)). The testicles were harvested immediately after the slaughter at the Bodija abattoir and transported to the laboratory in an insulated flask containing warm water at 37°C within 30 minutes. In the laboratory, the samples were dissected and histological sections of the right testis, right and left caudal epididymides taken from two bulls in each group. The tissues were fixed in Bouin’s fluid. They were processed in an automatic tissue processor, embedded in paraffin wax using the embedding system (Leica EG 1160) and sectioned with microtome at 4 microns. The sections were then stained by Haematoxylin and Eosin method and mounted and examined under a microscope. There was no significant difference between breed and seminiferous tubular diameter (STD) and testicular germinal height (TGEH). The mean STD ranged from 223.00 ± 28.35 to 316.00 ± 37.70 μm, while the TGEH ranged from 81.60 ± 10.05 to 89.80 ± 4.83 μm. The mean epididymal tubular diameters (ETD) and epididymal lumen diameters (ELD) had the highest value of 378.00 ± 10.95 and 298.20 ± 26.0489 μm in DL and lowest of 373.80 ± 37.70 and 278.10 ± 27.71 μm in RB, respectively. The mean epididymal germinal epithelia heights (EGEH) were highest (61.20 ± 5.70 μm) in WF and lowest (39.80 ± 0.86 μm) in RB. The mean sperm head length (SHL), sperm midpiece length (SMPL), sperm tail length (STL) and sperm total length (TL) had the highest values of 5.90 ± 0.10, 8.80 ± 0.25, 24.20 ± 2.59 and 38.90 ± 2.94 μm, respectively in SG. SHL and SMPL were lowest in DL bulls (5.00 ± 0.00 and 6.30 ± 0.20 μm), while STL and TL were lowest (18.90 ± 0.24

关键词： Histomorphometry testes Epididymis Spermatozoa Indigenous Bull

Inhibitory Effect of Aqueous Extract of Moringa oleifera and Newbuoldia laevis Leaves on Ferrous Sulphate and Sodium Nitroprusside Induced Oxidative Stress in Rat’s testes in Vitro

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Open Journal of Medicinal Chemistry 2012年第4期2卷 119-128页

作者： Seun F. Akomolafe Ganiyu Oboh Afolabi A. Akindahunsi Ayodele J. Akinyemi Olusola Adeyanju Department of Biochemistry Afe Babalola University Ado Ekiti Nigeria Department of Biochemistry Ekiti State University Ado Ekiti Nigeria Department of Biochemistry Federal University of Technology Akure Nigeria Department of Chemistry University of Jos Jos Nigeria

Oxidative stress has been identified as one of the factors that affects fertility status. Therefore, this study sought to investigate the inhibitory effect of aqueous extract of Moringa oleifera and Newbuoldia laevis leaves on FeSO4 and Sodium Nitroprusside (SNP) induced lipid peroxidation in rat testes in vitro. Incubation of the testes tissue homogenate in the presence of FeSO4 and SNP caused a significant increase in the malondialdehyde (MDA) contents of the testes. The aqueous extract from both Moringa oleifera and Newbuoldia laevis leaves caused a significant decrease in the MDA contents of the testes in a dose-dependent manner. However, aqueous extract from Moringa oleifera leaf (EC50 = 0.29 mg/ml) had a significant (P2+ induced lipid peroxidation in the rat testes homogenate than that of Newbuoldia laevis leaf extract (EC50 = 0.58 mg/ml);while there was no significant (P2+ chelating and reducing power. Therefore, these plants have potential to prevent oxidative stress in testes and improve fertility outcomes.

关键词： Lipid Peroxidation Malondialdehyde Antioxidant Activity testes