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Comparative Characterization of Total Flavonol Glycosides and terpene lactones in Different Ages,Cultivation Sources and Gender of Ginkgo biloba Leaves

Comparative Characterization of Total Flavonol Glycosides an...

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海峡两岸暨CSNR全国第十届中药及天然药物资源学术研讨会

作者： Yuping Tang~1,Xin Yao~1,Erxin Shang~1,Guisheng Zhou~1,Sheng Guo~1,Shulan Su~1,Chun Jin~2,Dawei Qian~1,Yong Qin~2 and Jin-ao Duan~(1,*) (1 Jiangsu Key Laboratory for High Technology of TCM formulae Research,Nanjing University of Chinese Medicine, Nanjing 210046,China 2 Jiangsu Shenlong Pharmaceutical Co.,Ltd.,Yancheng 224200,China)

Extract from Ginkgo biloba leaves has become a very popular plant medicine and herbal supplement for its potential benefit in alleviating symptoms associated with peripheral vascular disease,dementia,asthma and *** researches on *** leaves focus on the leaves collected on July and August from the tree of 4-7 years,a large number of 1 eaves from fruit cultivars(t he t ree of a bove 10 years) are ignored and be come obs olete a fter fru it h arvest s eason (November).In this paper,we expand the tree age range(from 1 to 300 years) and first comparatively analyze the total flavonol glycosides and terpene lactones in different ages,cultivation sources and gender of *** leaves collected in November by using the validated HPLC-ELSD and HPLC-PDA *** results showed that the contents of t otal terpene lactones and flavonol glycosides in the leaves of young ginkgo tree are higher than that in old tree,and they were higher in male trees than those in *** geographical factors appear to have significant influence on their contents. All the results will provide good basis for c omprehensive utilization of *** leaves,especially the leaves from fruit cultivars.

关键词： Ginkgo biloba leaves terpene lactone flavonol glycoside HPLC-ELSD HPLC-PDA

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Simple Method for Simultaneous Quantitation and Fingerprint Analysis of Ginkgo biloba Tablets by High Performance Liquid Chromatography-UV-Evaporative Light Scattering Detector

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Chemical Research in Chinese Universities 2009年第1期25卷 25-30页

作者： WANG Shu-fang LUO Xiao-ling SHAO Qing CHENG Yi-yu QU Hai-bin Department of Chinese Medicine Science and Engineering College of Pharmaceutical Sciences Zhejiang University Hangzhou 310058 P. R. China

By optimizing the separation and analytical conditions, a reliable, simple and accurate high-performance liquid chromatography（HPLC） method coupled with ultraviolet（UV） and evaporative light scattering detector（ELSD） was developed for the simultaneous determination of lactones and flavonoid, that is, bilobalide, ginkgolide A, ginkgolide B, and rutin, in more than 10 batches of Ginkgo biloba tablets from different pharmaceutical companies. The method could also be applied to fingerprint research, for a more general evaluation of the quality of this preparation. The separation of the components was achieved on a Hanbon C18 column with gradient elution using water and methanol containing 0.1% trifluoroacetic acid（TFA）. The column temperature was 30 ℃ and the flow-rate of the mobile phase was 0.6 mL/min. The drift tube temperature of the ELSD was set at 100 ℃, and the nitrogen flow-rate was 2 L/min. Good linear relationships were shown with correlation coefficients for analytes exceeding 0.9913. The limits of detection（LODs, S/N=3） and the limits of quantitation（LOQs, S/N=10） were 0.00887-0.0508 μg/μL and 0.0171- 0.0636 μg/μL, respectively, on the column. The relative standard deviations（RSD） of the analytes were less than 3.61% for intraday and 3.70% for interday, respectively. The average recovery rates obtained were in the range of （97.3±4.3）% to （101.9±3.1）% for all the compounds. The results of quantitative and fingerprint analysis proved that the contents of the components were totally similar in the preparation of Ginkgo biloba tablets from the same pharmaceutical company; whereas, they varied significantly in the preparations of Ginkgo biloba tablets from different companies.

关键词： Ginkgo biloba terpene lactone Flavonoid Fingerprint HPLC-UV-ELSD

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