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technetium-99m-labeled annexin V imaging for detecting prosthetic joint infection in a rabbit model

The Journal of Biomedical Research 2015年第3期29卷 224-231页

作者： Cheng Tang Feng Wang Yanjie Hou Shanshan Lu Wei Tian Yan Xu Chengzhe Jin Liming Wang Department of Orthopedic Surgery Nanjing First HospitalNanjing Medical University Department of Nuclear medicine Nanjing First HospitalNanjing Medical University Department of Radiation Oncology the First Affiliated Hospital of Shanxi Medical University Department of Radiology the First Affiliated Hospital of Nanjing Medical University

Accurate and timely diagnosis of prosthetic joint infection is essential to initiate early treatment and achieve a favorable outcome. In this study, we used a rabbit model to assess the feasibility of technetium-99m-labeled annexin V for detecting prosthetic joint infection. Right knee arthroplasty was performed on 24 New Zealand rabbits. After surgery, methicillin-susceptible Staphylococcus aureus was intra-articularly injected to create a model of prosthetic joint infection （the infected group, n = 12）. Rabbits in the control group were injected with sterile saline （n= 12）. Seven and 21 days after surgery, technetium-99m-labeled annexin V imaging was per- formed in 6 rabbits of each group. Images were acquired 1 and 4 hours after injection of technetium-99m- labeled annexin V （150 mBq）. The operated-to-normal-knee activity ratios were calculated for quantitative ana- lysis. Seven days after surgery, increased technetium-99m-labeled annexin V uptake was observed in all cases. However, at 21 days a notable decrease was found in the control group, but not in the infected group. The operated-to-normal-knee activity ratios of the infected group were 1.84 ±0.29 in the early phase and 2.19 ±0.34 in the delay phase, both of which were significantly higher than those of the control group （P=0.03 and P=0.02）. The receiver operator characteristic curve analysis showed that the operated-to-normal-knee activity ratios of the delay phase at 21 days was the best indicator, with an accuracy of 80%. In conclusion, technetium- 99m-labeled annexin V imaging could effectively distinguish an infected prosthetic joint from an uninfected prosthetic joint in a rabbit model.

关键词： prosthetic joint infection annexin V technetium-99m diagnosis

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Leukocyte-technetium-99m uptake in Crohn's disease:Does it show subclinical disease?

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World Journal of Gastroenterology 2010年第3期16卷 365-371页

作者： Luciene G mota Luiz GV Coelho Carlos JR Simal maria LA Ferrari Clodomiro Toledo Josep martin-Comin Simone OF Diniz Valbert N Cardoso Nuclear Engineering Department Federal University of Minas Gerais Gastro-enterology ALFA Institute Clinical HospitalFederal University of Minas Gerais Nuclear medicine Felício Rocho HospitalMedicine FacultyFederal University of Minas Gerais medicine Nuclear Center Bellvitge Hospital Radioisotope Laboratory Pharmacy FacultyFederal University of Minas Gerais

AIm:To evaluate inflammatory activity in patients with Crohn's disease (CD) using technetium-99m-hexamethylpropyleneamine oxime (99mTc-HmPAO) granulocyte ***: Twenty patients (7 male and 13 female) with CD and five healthy volunteers were selected for 99mTc-HmPAO granulocyte scintigraphy. The Crohn's Disease Activity Index (CDAI), blood tests and C-reactive protein (CRP) of each patient were performed 7 d before the scintigraphic images. The leukocytes were labeled according to the International Society of Radiolabeled Blood Elements (ISORBE) consensus protocol and the scintigraphic images, including single photon emission computed tomography, were obtained 30 min and 2 h after injection of the radiolabeled ***:The labeling yield of the leukocytes with the lipophilic complex 99mTc-HmPAO was 55.0%±10%. Six of the 20 patients (30%) presented congruent results for the three parameters investigated (CDAI, Scintigraphic Index and CRP). On the other hand, 14 patients (70%) did not show congruent results. There was no significant correlation between the indices analyzed according to the Spearman test (P>0.05,n=20).CONCLUSION: The results suggest that 99mTc-HmPAO-labeled leukocyte scintigraphy could be important for determining inflammatory activity in CD even in the absence of clinical symptoms.

关键词： technetium-99m Hexamethylpropyleneamine oxime Leukocytes Inflammatory bowel disease Scintigraphy

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Anti-sense oligonucleotide labeled with technetium-99m using hydrazinonictinamide derivative and N-hydroxysuccinimidyl S-acetylmercaptoacetyltriglycline: A comparison of radiochemical behaviors and biological properties

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World Journal of Gastroenterology 2008年第14期14卷 2235-2240页

作者： Yun-Chun Li Tian-Zhi Tan Jian-Guo Zheng Chun Zhang Department of Nuclear medicine West China Hospital Sichuan University 37 Guoxue Alley Chengdu 610041 Sichuan Province China

AIm:To explore and compare the radiochemical behavior and biological property of anti-sense oligonuc-leotide (ASON) labeled with technetium-99m using N-hydroxysuccinimidyl S-acetylmercaptoacetyltriglycl ine (NHS-mAG3) and hydrazinonictinamide derivative (HYNIC). mETHODS:After HYNIC and NHS-mAG3 were synthesized, ASON was labeled with technetium-99m using HYNIC and NHS-mAG3 as a bifunctional chelator. The in vivo and in vitro stability, binding rates of labeled compounds to serum albumen, biodistribution of 99mTc-mAG3-ASON and 99mTc-HYNIC-ASON in BALB/C mouse and its HT29 tumor cellular uptake were compared. RESULTS:The labeling efficiency and stability of 99mTc-mAG3-ASON were significantly higher than those of 99mTc-HYNIC-ASON (P = 0.02, and P = 0.03, respectively). 99mTc-mAG3-ASON had a significantly lower rate of binding to serum albumen than 99mTc-HYNIC-ASON (P 99mTc-HYNIC-ASON, the biodistribution of 99mTc-mAG3-ASON was significantly lower in blood, heart, liver and stomach (P 0.05) and significantly higher in lung and kidney (P mor cellular uptake rate of 99mTc-mAG3-ASON was significantly higher than that of 99mTc-HYNIC-ASON (P 99mTc-mAG3-ASON shows superior radiochemical behaviors and biological properties than 99mTc-HYNIC-ASON. 99mTc-mAG3-ASON is a potential radiopharmaceutical agent for in vivo application.

关键词： Anti-sense oligonucleotide Radiolabeling technetium-99m N-hydroxysuccinimidyl S-acetylmercapt oacetyltriglycline Hydrazinonictionamide derivative

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Guava extract (Psidium guajava) alters the labelling of blood constituents with technetium-99m

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2006年第6期7卷 429-435页

作者： ABREU P.R.C. ALmEIDA m.C. BERNARDO R.m. BERNARDO L.C. BRITO L.C. GARCIA E.A.C. FONSECA A.S. BERNARDO-FILHO m. Department of Biophysics and Biometry Institute of Biology Roberto Alcantara Gomes University of Estate of Rio de Janeiro Rio de Janeiro 20551030 Brazil Department of Physiology Biological Sciences and Health Center Federal University of Sergipe So Cristóvo Sergipe 49100000 Brazil Department of Pharmacology and Psychobiology Institute of Biology Roberto Alcantara Gomes University of Estate of Rio de Janeiro Rio de Janeiro 20551030 Brazil

Psidium guajava (guava) leaf is a phytotherapic used in folk medicine to treat gastrointestinal and respiratory dis-turbances and is used as anti-inflammatory medicine. In nuclear medicine, blood constituents (BC) are labelled with techne-tium-99m (99mTc) and used to image procedures. However, data have demonstrated that synthetic or natural drugs could modify the labelling of BC with 99mTc. The aim of this work was to evaluate the effects of aqueous extract of guava leaves on the labelling of BC with 99mTc. Blood samples of Wistar rats were incubated with different concentrations of guava extract and labelled with 99mTc after the percentage of incorporated radioactivity (%ATI) in BC was determined. The results suggest that aqueous guava extract could present antioxidant action and/or alters the membrane structures involved in ion transport into cells, thus decreasing the radiolabelling of BC with 99mTc. The data showed significant (Pm blood incubated with guava extract.

关键词： Antioxidant Blood constituents Psidium guajava technetium-99m

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Direct technetium-99m labeling of anti-hepatoma monoclonal antibody fragment:a radioimmunoconjugate for hepatocellular carcinoma imaging

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World Journal of Gastroenterology 2000年第3期6卷 348-352页

作者： Hui Jie Bian Zhi Nan Chen Jing Lan Deng Cell Engineering Research Center Basic Medical.DepartmentFourth Military Medical UniversityXi’an 710032Shaanxi ProvinceChina Department of Clinical Nuclear medicine Xijing HospitalXi’an 710033Shaanxi ProvinceChina

AIm To directly radiolabel an anti-hepatomamAb fragment HAb18 F（ab’）2 with 99mTc bystannous-reduced method,and assess thestability,biodistribution and radioimmun-oimaging（RⅡ）.mETHODS Immunoreactive fraction wasdetermined according to Lindmo’s ***’s reagent was used to determine thenumber of thiols in the reduced F（ab’）2.Labelingefficiency and homogeneity were measured bypaper chromatography,sodium dodecylsulphatepolyacrylamide gel electrophoresis（SDS-PAGE）and *** assay involvedthe incubation of aliquots of labeled antibody inethylenediaminetetraacetate（ EDTA ）and L-cysteine（L-cys）solutions with different molarratio at 37℃ for 1h,*** vivo utilized nude mice bearing humanhepatocellular carcinoma（HHCC）xenograftswith gamma camera imaging and tissuebiodistribution studies at regular *** The labeling procedure was finishedwithin 1.5 h compared with the'pretinning'method which would take at least *** vitrostudies demonstrated that the radiolabeled mAbfragment was homogeneous and retained *** studies indicatedthat 99mTc-labeled HAb18 F（ab’）2 in EDTA is morestable than in *** and biodistribution showed a significant tumor uptake at 24 h post-injection of 99mTc-labeled HAb18 F（ab’）2.Theblood,kidney,liver and tumor uptakes at 24hwere 0.56±0.09,56.45±11.36,1.43±0.27 and6.57±3.01（%ID/g）,*** 99mTc-HAb18 F（ab’）2 conjugateprepared by this direct method appears to be aneffective way to detect hepatoma in nude micemodel.

关键词： antibody monoclonal antibody fragments technetium-99m hepatocellular carcinoma liver neoplasms radioimmunoimaging

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Classification decision tree algorithm assisting in diagnosing solitary pulmonary nodule by SPECT/CT fusion imaging

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Journal of Pharmaceutical Analysis 2008年第2期20卷 119-124页

作者： Qiang Y. Guo Y. Jin C. Liu m. Yang A. Wang Q. Niu G. Imaging Center First Affiliated Hospital Medical School of Xi'an Jiaotong University Xi'an 710061 China Imaging Center Second Affiliated Hospital Medical School of Xi'an Jiaotong University Xi'an 710004 China

Objective To develop a classification tree algorithm to improve diagnostic performances of 99mTc-mIBI SPECT/CT fusion imaging in differentiating solitary pulmonary nodules(SPNs).methods Forty-four SPNs,including 30 malignant cases and 14 benign ones that were eventually pathologically identified,were included in this prospective *** patients received 99Tcm-mIBI SPECT/CT scanning at an early stage and a delayed stage before *** predictor variables,including 11 clinical variables,4 variable...

关键词： Decision tree Fusion imaging Solitary pulmonary nodule technetium-99m

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LABELLING OF mETALLOTHIONEIN WITH ^(99m)Tc

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Nuclear Science and Techniques 1992年第4期3卷 268-274页

作者：李毕忠吴永慧刘元方李军董燕兵王重庆茹炳根韩斌田嘉禾 Peking University Beijing 100871 China General Hospital of P.L.Army Beijing 100853 China

99mTc-labelled metallothionein (99mTc-mT) was prepared through both direct and transcomplexation labelling approaches. Buffer systems and a variety of other parameters in the direct labelling procedure were studied in detail. High radiolabelling yields of 93-96% and the specific radioactivity of 3.7 mB/μg were obtained under optimal conditions by the direct labelling method. The prepared 99mTc-mT was stable in vitro. The chelate-exchange kinetics of mT with 99mTc-glucohepatonate and 99mTc-citrate were also studied. Biodistribution and imaging studies showed that 99mTc-mT was accumulated in the animal kidneys at an exceptionally high level, indicating that 99mTc-mT might be a potential renal imaging agent.

关键词： metallothionein technetium-99m Radiolabelling Biodistribution Imaging

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Pre-clinical evaluation of a new indirectly labeled 99mTc-6-hydrazinopyridine-3-carboxylic acid （HYNIC）-depreotide with HYNIC as bifunctional chelator

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Chinese medical Journal 2012年第14期125卷 2538-2542页

作者： YU Fei LU ming-li ZHANG Xiao-ping FU Da HOU min CAI Hai-dong LI Dan WANG Jian YUAN Xue-yu LU Zhong-wei DONG Feng Department of Nuclear medicine Shanghai Tenth People'sHospital Tongji University School of Medicine Shanghai 200072China Department of General Surgery Shanghai Ruijin HospitalShanghai Jiao Tong University School of Medicine ShanghaiMinimally Invasive Surgery Center Shanghai 200025 China

Background technetium-99m or 99mTC is widely used for labeling peptide in nuclear medicine. Somatostatin and its analog can inhibit tumor cell growth after binding with its receptor. This research was to study the preclinical effect of a new 99rnTc-6-hydrazinopyridine-3-carboxylic acid （HYNIC）-depreotide, indirect 99rnTc labeling of depreotide using HYNIC as a bifunctional chelator. methods The cyclopeptide, cyclo-[（N-me） Phe-Tyr-D-Trp-Lys-VaI-Hcy], the linear peptide, and [CICH2-CO.^-Dap-Lys- Cys-Lys-amide] were synthesized by Fmoc solid-phase synthesis. The cyclopeptide and the linear peptide were linked by liquid-phase synthesis. The product depreotide was isolated and purified by high performance liquid chromatography and was confirmed by mass spectrography. Depreotide was labeled with egmTc through a direct labeling method, using HYNIC as a bifunctional chelator. Paper chromatography method was used to calculate the labeling rate, and through the comparative analysis selected the best mark conditions. The new 99mTc-HYNIC-depreotide was tested by high-performance liquid chromatography （HPLC）. The internalization and externalization rates of the new 99mTc-HYNIC-depreotide were studied in A549 cells. Furthermore, biodistribution of the radiopeptide was studied in nude mice, bearing tumors from human lung carcinoma cells SPC-A1. Results The molecular of synthesize depreotide was 1358, and the purity of it was 95.29%. The labeling efficiency of 99mTc-HYNIC-depreotide was highest at pH 6.0 and 15℃, about （70.95±0.84）%. The labeling rate of the new 99mTc-HYNIC-depreotide rose to a peak of （20.75±0.48）% at 60 minutes in A549 cells at 37℃ and decreased slightly later, while it elevated gradually during the time course at 4℃ and 25℃. The internalization rate of the new 99rnTc-HYNIC-depreotide at 37℃ increased gradually and reached the peak of 84.4% in 120 minutes, while the externalization rate of the new 99mTc-HYNIC-depreotide was always less than 20%. In mice bearing t

关键词： technetium-99m 6-hydrazinopyridine-3-carboxylic acid," depreotide lung carcinoma

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Experimental studies on imaging of infected site with 99m^Tc-labeled ciprofloxacin in mice

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Chinese medical Journal 2009年第16期122卷 1907-1909页

作者： ZHANG Hong JIANG Ning-yi ZHU Lin Department of Nuclear medicine Second Affiliated Hospital of Sun Yat-Sen University Guangzhou Guangdong 510120 China Department of Chemistry State Education Ministry Key Laboratory of Radiopharmaceuticals Beijing Normal University Beijing 100875 China

Background Bacterial infection can pose a substantial diagnostic dilemma. 99m^Tc-labeled ciprofloxacin （CPF） was developed as a biologically active radiopharmaceutical to diagnose infection. In the present research, we studied the biodistribution and imaging properties of infection tracer 99m^Tc-CPF in a mouse model of infection. methods CPF was labeled with 99m^Tc and the radiochemical purity and labeling rate were measured. A mouse model of infection was established. We then determined the biodistribution of 99m^Tc-CPF and conducted the whole body scintigraphy of the animal model. Results 99m^Tc-Ciprotech was stable for at least 6 hours at room temperature. The labeling rate of CPF by 99m^Tc was over 90%. Clearance of radioactivity mainly occurred in the liver and kidney, and the clearance from blood was rapid. Both biodistribution and imaging results showed higher uptake of 99m^Tc-CPF at sites of infection. The infectious tissue/normal tissue ratio peak was 4.30 at 4 hours after injection. Conclusions 99m^Tc-CPF is a sensitive radiopharmaceutical for scintigraphy of infectious lesions and it is easy to prepare.

关键词： ciprofloxacin technetium-99m infection

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Simulating the Production of medical Radioisotopes in a Fast Thorium-Cycle ADS with SERPENT

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World Journal of Nuclear Science and Technology 2021年第1期11卷 43-64页

作者： Ranjana Nath-m International Thorium Energy Committee (iThEC) Geneva Switzerland

Radiopharmaceuticals are used in nuclear medicine for diagnostic or therapeutic acts. The short decay half-lives of medical radioisotopes, especially those used for diagnostics, imply that they should be produced continuously and transported as quickly as possible to the medical units where they are used. Neutron-rich medical radioisotopes are generally produced in research reactors, like technetium-99m, lutetium-177, holmium-166 and iodine-131. On the other hand, proton-rich radioisotopes are produced via reactions with charged particles from accelerators like fluorine-18, gallium-67, iodine-123 and thallium-201. Beside this, innovative nuclear reactors are advocated as solutions to the issues of nuclear waste production and proliferation threats. Fast neutron, thorium-cycle and accelerator-driven subcritical (ADS) reactors are some of the most promising of them, proposed as safer fuel breeders and “waste burners”. This article examines the use of a fast thorium-cycle ADS with liquid lead-bismuth eutectic coolant for the production of molybdenum-99/technetium-99m and lutetium-177. Burnup simulation has been made with the monte-Carlo (mC) code SERPENT. It is demonstrated that mC codes can advantageously be used to determine the optimal irradiation time for a given radioisotope in a realistic reactor core. It is also shown that fast thorium-cycle ADS is an economical option for the production of medical radioisotopes.

关键词： medical Radioisotopes molybdenum-99 technetium-99m Lutetium-177 Fast Reactor Thorium Cycle Subcritical Reactor ADS Spallation Activation Burnup SERPENT

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