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Development of amplified consensus genetic markers in taxodiaceae based on Cryptomeria japonica ESTs data

Journal of Forestry Research 2013年第3期24卷 503-508页

作者： LU Yong-quan JIA Qing TONG Zai-kang The Nurturing Station for the State Key Laboratory of Subtropical Silviculture Zhejiang Agriculture and Forestry University

Amplified consensus genetic marker （ACGM） is a PCR-based marker technique that uses primers designed within conserved regions of coding sequences. After a comparison of Cryptomeria japonica and Arabidopsis ESTs to search for conserved sequences, 237 single e-PCR products were obtained. We randomly selected 110 candidate ACGM markers to test. Of the 110 candidate ACGM markers tested, 106 yielded stable and clear PCR products in C. japonica. We then tested the utility of these 106 primer pairs in 10 species, representing 7 genera of Taxodi- aceae. The number of specific amplification primer pairs among those 10 species varied from 49 to 103 （or 46.2±97.2%）. The 106 primer pairs （ACGM loci） were high transferable to Cryptomeria fortunei Hooibrenk （97.2%） but were low in Metasequoia glyptostroboides （46.2%）. The number of PCR bands per primer pair ranged from 1.06 to 1.15, which means that most of the ACGM primers can obtain a single band within these 10 taxodiaceae species. In summary, our study shows that ACGM is a technique applicable for marker development even in species with limited sequence data.

关键词： ACGM taxodiaceae Cryptomeriajaponica EST database

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Cloning and sequence analysis of nine novel MYB genes in taxodiaceae plants

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Journal of Forestry Research 2014年第4期25卷 795-804页

作者： Yong-quan LU Qing JIA Zai-kang TONG The Nurturing Station for the State Key Laboratory of Subtropical Silviculture Zhejiang Agriculture and Forestry University

Myeloblastosis （MYB） is one of the largest transcribed factor families in plants. To gain an overall picture of the evolution of MYB genes in relict plants, we cloned nine novel MYB genes in taxodiaceae plants （ Taxodium distichum, Taxodium ascendens, Cryptomeria japonica var. Sinensis, Cryptomeria japonica cv. Araucarioides, Cryptomer Ja- ponica, Metasequoia glyptostroboides, Cunninghamia lanceolata, Tai- wania cryptomerioides and Glyptostrobus pensilis）. The deduced amino acid sequences for MYBs showed that the nine MYB proteins contained two DNA binding domains. The first domain is from amino acid position 29 to 78, wherein three tryptophanes at 33, 53 and 73 were separated by 19 amino acids, respectively. The second domain is from amino acid position 82 to 127, wherein three tryptophanes at 86, 105 and 124 were separated by 18 amino acids, respectively, whereas the first tryptophane at amino acid position 86 is replaced by a phenylalanine. The characteri- zation of these conserved domains at nine MYBs indicated that they all belong to the R2R3-MYB group. The secondary structure analysis showed that a-helix and 13-turn are the major motifs of the predicted secondary structure of MYBs. The three dimensional model of each MYB protein showed that the structure is like clip, making it more flexi- ble and mobile. The similarities between the nine MYB proteins in taxodiaceae were calculated. The highest identical value of 99% is be- tween CjsMYB, CjMYB and CjaMYB, whereas the lowest value of 82% is between TaMYB and C1MYB. According to the phylogenetic tree, the distances between different genera were relatively large whereas those within genera were relatively small. As expected, accessions of the same genus formed a subgroup before being grouped with other genera.

关键词： Myeloblastosis taxodiaceae clone analysis R2R3-MYB group

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On Cuticular Compressions of Glyptostrobus europaeus (taxodiaceae) from Kaydagul Formation (Lower Miocene) of the Central Kazakhstan$V. L. Komarov Botanical Institute! Russian Academy of Sciences,St Petersburg 19737

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Acta Botanica Sinica 2003年第6期45卷 673-680页

作者： Sergei Vasilyevich VICKULIN 马清温 Sergey Glebovich ZHILIN 李承森 V.L.Komarov Botanical Institute 聊城大学生物系聊城252059 中国科学院植物研究所北京100093

Numerous leaf compressions of Glyptostrobus europaeus (Brongn.) Ung. (taxodiaceae) are found in Aquitanian (Lower Miocene) lignified clay localities Bolattam and Akzhar in the Southern Turgay, on the right bank of the Dulygaly-Zhilanshik river (Central Kazakhstan). The finely preserved lignified compression remains of leafy shoots make micro-morphological investigation feasible. Comparative studies of the epidermal features both living G. pensilis C. Koch and a new finding of G. europaeus from Early Miocene of Kazakhstan and illustrate their certain difference. The cuticular organization and epidermal features of fossil leaves, which have been compared with these of 'the nearest living relative', G. pensilis, were studied by scanning electron microscopy (SEM) and light microscopy (LM). The fossil leaves of G. europaeus from Kazakhstan are distinguished by stronger, than in living species, G. pensilis, developed 'micro-papillae' ('Kristallucken'), visible in SEM as ring-like structures left on the outer surface of cuticle in both nonstomatal and stomatal zones.

关键词： conifers taxodiaceae stomata leaf cuticle Lower Miocene

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COMPARATIVE EPIDERMAL STUDIES BETWEEN FOSSIL AND EXTANT SPECIES OF GLYPTOSTROBUS (taxodiaceae)

COMPARATIVE EPIDERMAL STUDIES BETWEEN FOSSIL AND EXTANT SPEC...

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全国系统与进化植物学研讨会暨第九届系统与进化植物学青年研讨会

作者： MA Qing-Wen, LI Cheng-Sen, LI Feng-Lan Beijing Museum of Natural History, Beijing 100050, China Institute of Botany, Chinese Academic of Sciences, Beijing 100093, China College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China

As an endemic genus, Glyptostrobus has a unique living species, G. pensilis C. Koch, which is naturally distributed within the Chinese Provinces of Guangdong, Guangxi, Fujian and Yunnan. Leaves of G. pensilis are spirally arranged and comprise three morphotypes: linear, linear-subulate and scale-like. The epidermal structures on both the abaxial and adaxial surfaces of the three morphotypes are very similar. The epidermal cells are elongated within the non-stomatal areas, while they are rectangular with slighily rounded corners on the intrastomatal zones. Pits *** clearly seen in the walls of epidermal cells both on non-stomatal areas and on intrastomatal zones. Stomata on both the abaxial and adaxial outer and inner surfaces of the three morphotypes of leaves are very similar in structure. Stomata apertures are elliptic. The long axes of stomatal apertures are mostly parallel to the mid-vein in linear and linear-subulate leaves, but most stomata are oblique or perpendicular to the mid-vein in scale-like leaves. Guard cells have thickened walls, especially on outer margins. The thickened outer margins of cell walls are reniform in shape. The thickened walls of guard cells form polar lamellae that produce protruding and curved ends at the two poles of the stoma. Commonly the stomata have 5 or 6 subsidiary cells. Stomata are iocated on each side of the mid-vein and distributed on both the abaxial and adaxial surfaces of leaves. In linear leaves, the number of stomata on the abaxial surface is more than that on the adaxial surface and the stomatal band, about 0.3 mm in width, on the abaxial surface comprises 7-10 rows of stomata on each side of the mid-vein, while the band on the adaxial surface has only 1-3 rows of stomata. On each surfaces of the quadrangular linear-subulate leaves, the numbers and distribution of stomata are almost the same. The stomatal band comprises 4-6 rows of stomata and occupies about 0.2 mm in width. There are 2-5 rows of stomata in each s

关键词： taxodiaceae Glvptostrobus Cuticle Stoma

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