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Characterization and target gene analysis of microRNAs in the antennae of the parasitoid wasp Microplitis mediator

Insect Science 2021年第4期28卷 1033-1048页

作者： Shuang Shan Shan-Ning Wang Xuan Song Adel Khashaveh Zi-Yun Lu Khalid Hussain Dhiloo Rui-Jun Li Xi-Wu Gao Yong-Jun Zhang State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijingChina College of Plant Protection China Agricultural UniversityBeijingChina Institute of Plant and Environment Protection Beijing Academy of Agricultural and Forestry SciencesBeijingChina College of Plant Protection Agricultural University of HebeiBaodingHebeiChina IPM Center of Hebei Province Key Laboratory of Integrated Pest Management on Crops in Northern Region of North ChinaMinistry of AgriculturePlant Protection InstituteHebei Academy of Agricultural and Forestry SciencesBaodingHebeiChina Department of Entomology Faculty of Crop ProtectionSindh Agriculture UniversityTandojamPakistan

MicroRNAs(miRNAs),a class of non-coding single-strand RNA molecules encoded by endogenous genes,are about 21–24 nucleotides long and are involved in the post-transcriptional regulation of gene expression in plants and ***,the types and quantities of miRNAs in the different tissues of an organism are diverse,and these divergences may be related to their specific *** we have identified 296 known miRNAs and 46 novel miRNAs in the antennae of the parasitoid wasp Microplitis mediator by high-throughput ***-three miRNAs were predicted to target olfactory-associated genes,including odorant binding proteins(OBPs),chemosensory proteins,odorant receptors(ORs),ionotropic receptors(IRs)and gustatory *** these,17 miRNAs were significantly highly expressed in the antennae,four miRNAs were highly expressed both in the antennae and head or wings,while the remaining 12 miRNAs were mainly expressed in the head,thorax,abdomen,legs and ***,miR-9a-5p and miR-2525-3p were highly expressed in male antennae,whereas miR-1000-5p and novel-miR-13 were enriched in female *** 17 miRNAs highly expressed in antennae are likely to be associated with olfaction,and were predicted to target one OBP(targeted by miR-3751-3p),one IR(targeted by miR-7-5p)and 14 ORs(targeted by 15 miRNAs including miR-6-3p,miR-9a-5p,miR-9b-5p,miR-29-5p,miR-71-5p,miR-275-3p,miR-1000-5p,miR-1000-3p,miR-2525-3p,miR-6012-3p,miR-9719-3p,novel-miR-10,novel-miR-13,novel-miR-14 and novel-miR-28).These candidate olfactory-associated miRNAs are all likely to be involved in chemoreception through the regulation of chemosensory gene expression in the antennae of ***.

关键词： expression profile miRNA Microplitis mediator,olfactory-associated gene target gene

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Inhibitary effects of antisense oligonucleotide specific to K-ras point mutation on the target gene expression in human pancreatic carcinoma cells

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Chinese Medical Journal 2007年第16期120卷 1448-1450页

作者： WANG Yong-xiang GAO Liang JI Zong-zheng Department of general Surgery Zhejiang Provincial People＇s Hospital Hangzhou 310014 China Department of Oncology Zhejiang Provincial People＇s Hospital Hangzhou 310014 China Department of general Surgery Second Hospital of Xi＇an Jiaotong University Xi＇an 710004 China

The prognosis of pancreatic carcinoma is disappointing due to the difficulty of early and accurate diagnosis,low operative resection rate, insensitivity to radiation therapy and chemotherapy. The incidence of pancreatic carcinoma has increased considerably in the past few years, the progress of diagnosis and treatment, however, has not been significantly improved. The overall 5-year survive rate is still as low as 5%-10%, and the improved 5 years survive rate is about 20%-40% after successive Whipple-operation. The early diagnosis is critical to the successful surgical treatment. It depends on the establishment of the new way for that.

关键词： pancreatic carcinoma k-ras gene antisense oligonucleotides target gene

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Differential gene Expression Profiles in Coronary Heart Disease Patients of Blood Stasis Syndrome in Traditional Chinese Medicine and Clinical Role of target gene

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Chinese Journal of Integrative Medicine 2009年第2期15卷 101-106页

作者：马晓娟殷惠军陈可冀 Department of Cardiovascular Disease Xiyuan HospitalChina Academy of Traditional Chinese Medicine

Objective:To investigate the differential gene expression profiles in coronary heart disease(CHD) patients of blood-stasis syndrome(BSS) by oligonucleotide microarray technique,and the clinical significance of target ***:Subjects were assigned to CHD patients with BSS(n=8),CHD patients without BSS (n=8),and BSS patients without CHD(n=8) based on coronary angiography and the diagnostic criteria of BSS. The sex- and age-matched healthy volunteers(n=8) were enrolled as the control *** blood s...

关键词： coronary heart disease blood-stasis syndrome differential gene expression profile target gene

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Screening key target genes for severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)based on bioinformatics and gene network

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Precision Medicine Research 2020年第2期2卷 48-55页

作者： Zhi-Hua Yang Hai-Feng Yan Lin-Wang Miao-Ru Han First Teaching Hospital of Tianjin University of Traditional Chinese Medicine Tianjin 300381China

Background:To provide a reference for the clinical development of drugs to suppress severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).Methods:Retrieving genes related to SARS-CoV-2 with genecards database and then importing the obtained gene data into the database of Database for Annotation,Visualization and Integrated Discovery(DAVID)(Version 6.8)to collect relevant information on pathways and *** enriched in the first 20 most significant pathways and genes with gene occurrence frequency≥6 were respectively imported into the STRING database to construct protein-protein interaction(PPI)network diagrams,and the two network diagrams were ***:In the two network graphs,RELA,MAPK1,MAPK3,PIK3CA,PIK3R1,MAPK8,JAK1,STAT1,TNF,IL6,MAPK14,and IL1B ranked higher,and the occurrence frequency of the first 20 pathways was≥***:The pathogenesis of SARS-CoV-2 is associated with multiple pathways such as influenza A,TNF signaling pathway,chemokine signaling pathway,toll-like receptor signaling pathway,T cell receptor signaling pathway et ***,MAPK1,MAPK3,PIK3CA,PIK3R1,MAPK8,JAK1,STAT1,TNF,IL6,MAPK14 and IL1B are closely related to SARS-CoV-2 and need further *** interaction network and pathway analysis of diseaseassociated genes will help us to screen the key target genes of SARS-CoV-2 and provide a reference for the clinical development of effective drugs.

关键词： Bioinformatics gene network SARS-CoV-2 COVID-19 target gene

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Microarray-based Screening of target genes Regulated by Heat Shock Factor AtHsfA1a in Arabidopsis thaliana

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Agricultural Biotechnology 2016年第3期5卷 1-5,8页

作者： Lihong GUO Enyin YANG Nian LI Ya XU Jieyuan ZENG Key Laboratory of Special Biological Resource Development and Utilization of Universities in Yunnan Province Kunming University Kunming Key Laboratory of Hydroecology Restoration of Dianchi Lake Kunming University Department of Life Science and Technology Kunming University

[ Objective] This study aimed to screen target genes regulated by heat shock factor AtHsfAla in Arabidopsis thaliana. [ Method] Using AtHsfAla-in- serted mutant athsfala （SALK-068042） and wild-type A. thaliana seedlings as experimental materials, target genes regulated by heat shock factor AtHsfAla were screened by microarray assay. Differentially expressed genes were screened by multiple method. Specific functions of differentially expressed genes were analyzed by gene ontology （GO） analysis. Signal transduction pathways, in which differentia｜ly expressed genes were involved, were analyzed by pathway analysis. gene-gene interaction network was constructed by Signal-Net. [ Result] A total of 3 672 differentially expressed genes were screened out. Up-regulated differentially expressed genes were involved in 198 functions and 7 signal transduction pathways; down-regulated differentially expressed genes were involved in 94 functions and 10 signal transduction pathways. In the signal transduction network, it was found that cwlNV4 and HXK3 had relatively high ability of mediation; AT1 G14240 and cwlNV4 ex- hibited the most interactions with other genes, which were located in key positions throughout the gene-gene interaction network. [ Conclusion] Heat shock factor AtHsfAla regulates a large number of target genes in A. thaliana.

关键词： Arabidopsis thaliana Heat shock factor AtHsfA1 a Microarray target gene

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Analysis of parental strain DNA fragments existing in GEMs-Fhhh

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Journal of Environmental Sciences 2003年第5期15卷 590-594页

作者： HAOChun-bo YANJun QUMeng-meng WANGDong CHENGShu-pei GUJi-dong QIUWan-fei WANGYin-yin TheNationalKeyLaboratoryofPollutionControlandResourceReuse SchoolofEnvironmentalScienceNanjingUniversityNanjing210093China DepartmentofEcology&Biodiversity TheUniversityofHongKongPokfulamRoadHongKongChina

There were 6 target DNA fragments of the three parental strains existing in the cell of GEMs(genetically engineered microorganism strain) Fhhh measured in this research by PCR(polymerase chain reaction). The determination showed that GEMs Fhhh contained all the 6 target DNA fragments, mnp 1, mnp 2、 lip 1、 lip 2, FLO 1 and 16S rDNA, and had the molecular genetic stability. Meanwhile the PCR production of each parental strain could only had its target DNA fragments and was different from each other. It may illustrate that the technique of the inter kingdom protoplast fusion for the construction of GEMs Fhhh through the process of intercellular gene recombination could be used as a reliable bioengineering technique to create the specific functional stain for the pollution control.

关键词： GEMs protoplast fusion target gene DNA fragment PCR pollution control

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PuHox52 promotes coordinated uptake of nitrate,phosphate, and iron under nitrogen deficiency in Populus ussuriensis

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Journal of Integrative Plant Biology 2023年第3期65卷 791-809页

作者： Ming Wei Mengqiu Zhang Jiali Sun Ying Zhao Solme Pak Miaomiao Ma Yingxi Chen Han Lu Jingli Yang Hairong Wei Yuhua Li Chenghao Li State Key Laboratory of Tree genetics and Breeding School of ForestryNortheast Forestry UniversityHarbin 150040China College of Life Sciences Northeast Forestry UniversityHarbin 150040China College of Forest Resources and Environmental Science Michigan Technological UniversityHoughtonMichigan 49931USA Key Laboratory of Saline-alkali Vegetation Ecology Restoration(Northeast Forestry University) Ministry of EducationHarbin 150040China

It is of great importance to better understand how trees regulate nitrogen(N) uptake under N deficiency conditions which severely challenge afforestation practices, yet the underlying molecular mechanisms have not been well elucidated. Here,we functionally characterized PuHox52, a Populus ussuriensis HD-ZIP transcription factor, whose overexpression greatly enhanced nutrient uptake and plant growth under N deficiency. We first conducted an RNA sequencing experiment to obtain root transcriptome using PuHox52-overexpression lines of P. ussuriensis under low N treatment. We then performed multiple genetic and phenotypic analyses to identify key target genes of PuHox52 and validated how they acted against N deficiency under PuHox52 ***52 was specifically induced in roots by N deficiency, and overexpression of PuHox52promoted N uptake, plant growth, and root development. We demonstrated that several nitrate-responsive genes(PuNRT1.1, PuNRT2.4,PuCLC-b, PuNIA2, PuNIR1, and PuNLP1),phosphate-responsive genes(PuPHL1A and PuPHL1B), and an iron transporter gene(PuIRT1) were substantiated to be direct targets of PuHox52. Among them, PuNRT1.1, PuPHL1A/B, and PuIRT1 were upregulated to relatively higher levels during PuHox52-mediated responses against N deficiency in PuHox52-overexpression lines compared to WT. Our study revealed a novel regulatory mechanism underlying root adaption to N deficiency where PuHox52 modulated a coordinated uptake of nitrate, phosphate, and iron through 'PuHox52-PuNRT1.1', 'PuHox52-PuPHL1A/PuPHL1B', and'PuHox52-PuIRT1' regulatory relationships in poplar roots.

关键词： HD-Zip transcription factor iron transport nitrate transport nitrogen deprivation phosphate absorption Populus ussuriensis root architecture target gene

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Discovery of MicroRNAs Associated with the S Type Cytoplasmic Male Sterility in Maize

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Journal of Integrative Agriculture 2013年第2期12卷 229-238页

作者： YU Jiang-hua ZHAO Yan-xin QIN Ya-ting YUE Bing ZHENG Yong-lian XIAO Hai-lin National Key Laboratory of Crop genetic Improvement/Huazhong Agricultural University

MicroRNAs （miRNAs） are endogenous small RNAs that play important regulatory roles in the growth and development processes of plants and animals. In this study, we examined the expression profiles of pollen miRNAs from a maize S type cytoplasmic male sterile line and its fertility restored line. In total, 100 known miRNAs belonging to 20 families and 81 novel miRNAs belonging to 44 families were identified. Two and seven known miRNAs had significant expression difference between the two lines at the level of P-value〈0.01 and 0.01〈P-value〈0.05, respectively. Four miRNAs showing 〉1.5 fold expression difference were verified by stem-loop RT-qPCR. gene Ontology analysis of miRNA target genes revealed that these genes mainly participated in the transcriptional regulation processes.

关键词： CMS-S microRNAs real-time PCR Solexa sequencing target gene maize

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High-throughput sequencing-based genome-wide identification of micro RNAs expressed in developing cotton seeds

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Science China(Life Sciences) 2015年第8期58卷 778-786页

作者： WANG YanMei DING Yan YU DingWei XUE Wei LIU JinYuan Laboratory of Plant Molecular Biology Center for Plant Biology School of Life Sciences Tsinghua University

Micro RNAs(mi RNAs) have been shown to play critical regulatory roles in gene expression in cotton. Although a large number of mi RNAs have been identified in cotton fibers, the functions of mi RNAs in seed development remain unexplored. In this study, a small RNA library was constructed from cotton seeds sampled at 15 days post-anthesis(DPA) and was subjected to high-throughput sequencing. A total of 95 known mi RNAs were detected to be expressed in cotton seeds. The expression pattern of these identified mi RNAs was profiled and 48 known mi RNAs were differentially expressed between cotton seeds and fibers at 15 DPA. In addition, 23 novel mi RNA candidates were identified in 15-DPA seeds. Putative targets for 21 novel and 87 known mi RNAs were successfully predicted and 900 expressed sequence tag(EST) sequences were proposed to be candidate target genes, which are involved in various metabolic and biological processes, suggesting a complex regulatory network in developing cotton seeds. Furthermore, mi RNA-mediated cleavage of three important transcripts in vivo was validated by RLM-5′ RACE. This study is the first to show the regulatory network of mi RNAs that are involved in developing cotton seeds and provides a foundation for future studies on the specific functions of these mi RNAs in seed development.

关键词： Gossypium hirsutum seed development microRNA (miRNA) target gene GO annotation high-throughput sequencing

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Identification and Characterization of ABA-Responsive MicroRNAs in Rice

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Journal of genetics and Genomics 2015年第7期42卷 393-402页

作者： Caijuan Tian Zhangli Zuo Jin-Long Qiu State Key Laboratory of Plant Genomics Institute of MicrobiologyChinese Academy of Sciences University of Chinese Academy of Sciences

MicroRNAs （miRNAs） are endogenous non-coding small RNAs that silence genes through mRNA degradation or translational inhibition. The phytohormone abscisic acid （ABA） is essential for plant development and adaptation to abiotic and biotic stresses. In Arabidopsis, miRNAs are implicated in ABA functions. However, ABA-responsive miRNAs have not been systematically studied in rice. Here high throughput sequencing of small RNAs revealed that 107 miRNAs were differentially expressed in the rice ABA deficient mutant, Osabal. Of these, 13 were confirmed by stem-loop RT-PCR. Among them, miR1425-5P, miR169a, miR169n, miR390-5P, miR397a and miR397b were up-regulated, but miR162b reduced in expression in Osabal. The targets of these 13 miRNAs were predicted and validated by gene expression profiling. Interestingly, the expression levels of these miRNAs and their targets were regulated by ABA. Cleavage sites were detected on 7 of the miRNA targets by 5＇-Rapid Amplification of cDNA Ends （5＇-RACE）. Finally, miR162b and its target OsTRE1 were shown to affect rice resistance to drought stress, suggesting that miR162b increases resistance to drought by targeting OsTRE1. Our work provides important information for further characterization and functional analysis of ABA-responsive miRNAs in rice.

关键词： Rice Abscisic acid High throughput sequencing MicroRNA target gene

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