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Protective Effect of tangshen formula on Interstitial Cells of Cajal in Colon of Diabetic Rats

Chinese Journal of Integrative Medicine 2022年第1期28卷 43-51页

作者： CHEN Peng-min ZHAO Jing-bo YANG Xin LI Jing ZHANG Hao-jun ZHANG Hui-cun ZHANG Yu-ting LI Ping Beijing Key Lab Immune-Mediated Inflammatory Diseases Institute of Clinical Medical SciencesChina-Japan Friendship HospitalBeijing(100029)China Department of Clinical Medicine Aarhus UniversityAarhus(8200)Denmark Department of Combination of Traditional Chinese and Western Medicine Beijing Hospital of Traditional Chinese MedicineCapital Medical UniversityBeijing(100010)China

Objective:To explore the effect of tangshen formula(TSF),a Chinese herbal medicine,on interstitial cells of Cajal(ICC)in the colon of diabetic ***:Fifty-four male Wistar rats were randomly divided into normal control(NC,n=14)and high-fat diet(HFD)groups(n=40).After 6 weeks,the rats in the HFD group were injected intraperitoneally streptozotocin once(30 mg/kg).Thirty rats with fasting blood glucose higher than 11.7 mmol/L were randomly divided into diabetes(DM)and TSF groups,15 rats in each *** in the NC and DM groups were intragastrically administered with saline,and those in the TSF group were given with TSF(2.4 g/kg)once daily for 20 *** levels of Bax,Bcl-2,and caspase-3 in colonic smooth muscle layer were measured by Western blotting and immunohistochemical *** number of ICC was determined by immunohistochemical *** was used for analyzing the ratio of classically activated macrophages(M1)and alternatively activated macrophages(M2)to total *** microscopy was used to observe the epithelial ultrastructure and ***:TSF appeared to partially prevented loss of ICC in DM rats(P<0.05).Compared with the NC group,expression levels of Bcl-2,Bax,caspase-3,and TNF-αas well as the ratio of M1 to total macrophages increased in DM rats(all P<0.05),and the ratio of M2 to total macrophages decreased(P<0.05 or P<0.01).Compared with the DM group,TSF decreased the expression levels of abovementioned proteins and restore M2 to total macrophages ratio(P<0.05 or P<0.01).TSF appeared to attenuate the ultrastructural changes of epithelia and improve the tight and desmosome junctions between epithelia reduced in the DM ***:Reduced number of ICC in DM rats may be associated with damage of the intestinal *** protective effects of TSF on ICC may be through repair of the epithelial junctions,which attenuates inflammation and inflammation-initiated apoptosis in colon of DM rats.

关键词： diabetes interstitial cells of Cajal tight junction intestinal epithelia macrophage tangshen formula

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