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THE　ULTRASTRUCTURE　OF　TOXOPLASMA　GONDII　tachyzoiteS　AND　THE　EFFECT　OF　USNIC　ACID　ON　IT

Journal of Pharmaceutical Analysis 1996年第1期10卷 91-94页

作者：吴杰杨少毅程彦斌

The normal fine structures of toxoplasma gondii tachyzoite and the erfect of usnic acid on the ultrastructure of the tachyzoites were observed by transmission electron microscope (TEM).The studies indicate that the pathological changes or the ultrastructure of the parasite took place under the effect of the 10 mg/L usnic acid for 30 *** changes can be illustrated as folio'vs; 1)The posterior portion of the rhopties were destroyed.2)The membrane of the daughter cell fractured. 3)The membranate organellae or the organisms were demaged.

关键词： toxoplasma gondii tachyzoite ultrastructure usnic acid

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Analysis Concentration of <i>Toxoplasma gondii</i>on Anti-Toxoplasma IgG-IgM Antibody Levels, and the Outcomes of Pregnancy in Mice Balb/c

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Open Journal of Obstetrics and Gynecology 2017年第3期7卷 281-289页

作者： Tigor Peniel Simanjuntak Mochammad Hatta Robert H. Sirait Marni Br Karo Lenny Irmawaty Sirait Tetty Rina Aritonang Syahrul Rauf Ressy Dwiyanti Department of Obstetrics and Gynecology Faculty of Medicine Christian University of Indonesia Jakarta Indonesia Molecular Biology and Immunology Laboratory Faculty of Medicine Hasanuddin University Makassar Indonesia Department of Anaestesiology Faculty of Medicine Christian University of Indonesia Jakarta Indonesia Midwife Program of Medistra Health Higher School Jakarta Indonesia Department of Obstetrics and Gynecology Faculty of Medicine Hasanuddin University Makassar Indonesia Department of Medical Microbiology Faculty of Medicine Tadulako University Palu Indonesia

Introduction: Toxoplasma gondii is an obligate intracellular protozoan parasite that can infect any warm blood vertebrae, and if first trimester pregnant woman infected, it may cause abortion. The objective is to prove the effect of the Toxoplasma gondii concentration in anti-toxoplasma IgG-IgM antibody levels, and the outcomes of Balb/c mice pregnancies. Materials and Methods: The study was conducted in Balb/c mice with inclusion criteria, and was conditioned pregnant. The pathogen strains of Toxoplasma gondii tachyzoite injected intraperitoneally. The blood samples were taken serially to be tested for anti-toxoplasma IgG-IgM antibody levels. After the mice were injected with tachyzoite, they are assessed every day to observe their body weight, vaginal bleeding, and labor. Anti-toxoplasma IgG-IgM antibody levels examined using qualitative mouse IgG-IgM antibody ELISA KIT. Results: Anti-toxoplasma IgM antibody levels increased significantly after 24 hours of injection tachyzoites in all dose groups, and remained high through day 21. Anti-toxoplasma antibody IgG levels increased significantly after 72 hours post injection and remained elevated until day 21. The incidence of abortion is 100% in mice which injected tachyzoite levels 1 × 103 and 1 × 104, and the incidence of abortion approximately 2 - 4 days post injection. 100% of mice that were injected with tachyzoites 1 × 101 and 1 × 102 have labor at term. Physical anomaly was found in baby mice from mice that were injected with tachyzoite 1 × 102. Conclusion: There is a significant correlation between the concentrations of Toxoplasma gondii tachyzoite with anti-toxoplasma IgG-IgM antibody levels, and there is a significant relationship between the concentrations of tachyzoite with abortion.

关键词： Toxoplasma gondii tachyzoite Anti-Toxoplasma IgG-IgM Antibody Levels Pregnancy Abortion Labor

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