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Salvianolic Acid B Inhibits the tlr4-nfκ B-tnfα pathway and Attenuates Neonatal Rat Cardiomyocyte Injury Induced by Lipopolysaccharide

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Chinese Journal of Integrative Medicine 2011年第10期17卷 775-779页

作者：王阶张云郭丽丽吴广均刘瑞华 Guang＇anmen Hospital China Academy of Chinese Medical Sciences Beijing （100053） China Hubei University of Traditional Chinese Medicine Wuhan （430065） China

Objective： To investigate the role of the tlr4-nf K B-tnfa inflammation pathway on lipopolysaccharide （LPS）-induced neonatal rat cardiomyocyte injury and the possible protective effects of salvianolic acid B （Sal B）. Methods： Wistar rat （1-2 days old） cardiomyocytes were isolated and cultured. Sal B 10-5mol/L, 10-6mol/L and 10-7mol/L were pro-treated for 6 h in the culture medium. LPS （1 μg/mL） was added to the culture medium and kept for 6 h to induce inflammation injury. The concentration of lactate dehydrogenase （LDH） in the supernatant was detected by spectrophotometry. The concentrations of tumor necrosis factor （tnf a） and heat shock protein 70 （HSP70） in the supernatant were detected by enzyme linked immunosorbent assay. The protein expressions of toll, such as receptor 4 （tlr4） and nuclear factor kappa B （nf K B） were detected by immunohistochemistry. The mRNA expressions of tlr4 and nf K B were detected by realtime reverse transcription polymerase chain reaction （RT-PCR）. Results：（1） The concentrations of LDH and tnf a in the LPS control group were significantly higher than those in the control group （561.41 ± 67,39 U/L and 77.94± 15.08 pg/mL, versus 292.13± 26.02 U/L and 25.39 ±16.53 pg/mL, respectively, P〈0.01, P〈0.05）. Compared with the LPS control group, the concentrations of LDH and tnf α were significantly decreased in the Sal B 10-5mol/L pro-treated group （451.76 ± 83.96 U/L and 34.00± 10.38 pg/mL, respectively, P〈0.05）. （2） The tlr4 and nf K B protein expression area in the LPS control group were significantly higher than those in the control group （1712.41 ± 410.12 μm2 and 2378.15 ± 175.29 μm2, versus 418.62 ± 24.42 μ m2 and 1721.74 ± 202.87μ m2, respectively, P〈0.01）. The tlr4 and nf K B protein expression internal optical density （IOD） values in the LPS control group were also significantly higher than those in the control group （3.06 ±0.33 and 7.20± 1.04, versus 0.91 ±0.21 and 4.24±0.48, respectively, P〈0.05 and P〈0.01）. Compared with the LPS c

关键词： salvianolic acid B tlr4-nf kb-tnf a pathway cardiomyocytes lipopolysaccharide

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