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甘青青兰质量标准的研究

中成药 2022年第6期44卷 1770-1773页

作者：范莹莹马青青杨凤梅张炜骆桂法青海省药品检验检测院青海省中藏药现代化研究重点实验室国家药品监督管理局中药藏药质量控制重点实验室青海西宁810016

目的建立甘青青兰的质量标准。方法 tlc法定性鉴别齐墩果酸、绿原酸,HPLC法测定绿原酸、胡麻素-6-O-葡萄糖苷含量。结果 tlc斑点清晰,分离度良好。绿原酸、胡麻素-6-O-葡萄糖苷分别在0.23~2.33、0.17~1.67μg范围内线性关系良好(r=0.99... 详细信息

目的建立甘青青兰的质量标准。方法 tlc法定性鉴别齐墩果酸、绿原酸,HPLC法测定绿原酸、胡麻素-6-O-葡萄糖苷含量。结果 tlc斑点清晰,分离度良好。绿原酸、胡麻素-6-O-葡萄糖苷分别在0.23~2.33、0.17~1.67μg范围内线性关系良好(r=0.999 9),平均加样回收率分别为102.8%、98.3%,RSD分别为0.5%、0.9%。结论该方法简便可靠,可用于甘青青兰的质量控制。

关键词：甘青青兰齐墩果酸绿原酸胡麻素-6-O-葡萄糖苷 tlc HPLC

DETERMINATION OF COMPOSITION DISTRIBUTION OF SEGMENTED POLYURETHANES BY THIN LAYER CHROMATOGRAPHY

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Chinese Journal of Polymer Science 1986年第1期4卷 78-83页

作者：徐懋李培基王瑛罗筱烈刘彦马德柱 Academia Sinica Anhui Beijing Department of Applied Chemistry China University of Science and Technology of China Hefei Institute of Chemistry

The compositional heterogeneity has been studied for a series of PCL/MDI/BDO segmentedpolyurethanes. Their average hard segment content measured by NMR method was ranging from17% to 42%. Macromolecules of segmented polyurethanes can be separated according to theircomposition by tlc technique with a mixed solvent of toluene/acetone/ tetrahydrofuran as the devel-oper. A dual wavelength tlc spectrodensitometer was used for quantitative analysis of tlc results,and method for data treatment is proposed to obtain the composition distribution with no need ofany reference samples as tlc elution standards. The polyurethane samples are found to be hetero-geneous in composition, having bimodal distribution curves.

关键词： PCL DETERMINATION OF COMPOSITION DISTRIBUTION OF SEGMENTED POLYURETHANES BY THIN LAYER CHROMATOGRAPHY tlc

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心宝丸质量标准的提高

中成药 2022年第11期44卷 3639-3643页

作者：吴丽琼林琼英庄伟洪蔡荣钦广东心宝药业科技有限公司广东广州510535

目的提高心宝丸质量标准。方法采用tlc法定性鉴别洋金花,GC法定性鉴别冰片、人工麝香、肉桂,HPLC法测定人参皂苷Rg、人参皂苷Re、人参皂苷Rb总含量。结果药材定性鉴别专属性强,阴性无干扰。3种人参皂苷在各自范围内线性关系良好(r≥0... 详细信息

目的提高心宝丸质量标准。方法采用tlc法定性鉴别洋金花,GC法定性鉴别冰片、人工麝香、肉桂,HPLC法测定人参皂苷Rg、人参皂苷Re、人参皂苷Rb总含量。结果药材定性鉴别专属性强,阴性无干扰。3种人参皂苷在各自范围内线性关系良好(r≥0.999 5),平均加样回收率96.7%~97.4%,RSD 1.4%~2.8%,平均总含量为1.48 mg/丸。结论该方法准确可靠,专属性强,可为心宝丸质量标准的提高提供依据。建议每丸含人参、三七以人参皂苷Rg、人参皂苷Re、人参皂苷Rb总含量计,不得少于1.0 mg。

关键词：心宝丸药材人参皂苷Rg1 人参皂苷Re 人参皂苷Rb1 tlc GC HPLC

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藓生马先蒿质量标准研究

中成药 2023年第10期45卷 3342-3346页

作者：焦兴苹达洛嘉杨凤梅青海省药品检验检测院国家药品监督管理局中药(藏药)质量控制重点实验室青海省中藏药现代化研究重点实验室青海西宁810016

目的建立藓生马先蒿质量标准。方法采用显微鉴别、tlc进行定性鉴别,2020年版《中国药典》方法检测其水分、总灰分、酸不溶性灰分、浸出物含量,HPLC法测定京尼平苷酸、毛蕊花糖苷含量。结果显微鉴别特征明显,可见花粉粒、草酸钙晶体、网... 详细信息

目的建立藓生马先蒿质量标准。方法采用显微鉴别、tlc进行定性鉴别,2020年版《中国药典》方法检测其水分、总灰分、酸不溶性灰分、浸出物含量,HPLC法测定京尼平苷酸、毛蕊花糖苷含量。结果显微鉴别特征明显,可见花粉粒、草酸钙晶体、网纹状细胞、螺纹导管。tlc特征斑点分离清晰。10批样品水分、总灰分、酸不溶性灰分、浸出物平均含量分别为8.3%、15.8%、2.0%、36.7%。京尼平苷酸、毛蕊花糖苷分别在12.83~128.31、11.77~117.75μg/mL范围内线性关系良好(r≥0.9997),平均加样回收率分别为101%、101%,RSD分别为1.16%、1.08%,平均含量分别为0.31%、1.78%。结论藓生马先蒿质量标准为水分、总灰分、酸不溶性灰分分别不得超过15%、20%、4.0%,浸出物不得少于33.0%,京尼平苷酸、毛蕊花糖苷含量分别不得少于0.25%、1.70%。

关键词：藓生马先蒿京尼平苷酸毛蕊花糖苷 tlc HPLC 2020年版《中国药典》

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铧头草质量标准研究

中成药 2023年第4期45卷 1270-1274页

作者：沙马里牛李文兵沙冬梅陈燃陈丽萍倪帅聪阎新佳刘圆西南民族大学药学院四川成都610041 四川省羌彝药用资源保护与利用技术工程实验室四川成都610225 青藏高原民族药用资源保护与利用国家民委重点实验室四川成都610225

目的建立铧头草质量标准。方法采用显微鉴别、tlc对彝药材铧头草进行定性鉴别,HPLC法测定秦皮乙素含量,2020年版《中国药典》方法测定水分、总灰分、酸不溶性灰分、浸出物含量。结果显微鉴别特征明显,可见薄壁细胞、纤维束、螺纹导管、... 详细信息

目的建立铧头草质量标准。方法采用显微鉴别、tlc对彝药材铧头草进行定性鉴别,HPLC法测定秦皮乙素含量,2020年版《中国药典》方法测定水分、总灰分、酸不溶性灰分、浸出物含量。结果显微鉴别特征明显,可见薄壁细胞、纤维束、螺纹导管、草酸钙簇晶、淀粉粒等。早开堇菜、心叶堇菜tlc特征斑点分离清晰。水分、总灰分、酸不溶性灰分含量分别不得超过13%、24%、7%,浸出物含量不得低于10%。秦皮乙素在0.05~0.80 mg/mL范围内线性关系良好(R^(2)=0.9995),平均加样回收率为100.17%、RSD为1.07%,早开堇菜中该成分含量不得低于0.10%,而心叶堇菜中不限。结论该方法简便、准确、重复性好,可用于铧头草的质量控制。

关键词：铧头草早开堇菜心叶堇菜秦皮乙素显微鉴别 tlc HPLC 2020年版《中国药典》

Analysis of phytochemical profile of Terminalia arjuna bark extract with antioxidative and antimicrobial properties

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Asian Pacific Journal of Tropical Biomedicine 2013年第12期3卷 960-966页

作者： Shreya Mandal Arpita Patra Animesh Samanta Suchismita Roy Arpita Mandal Tapasi Das Mahapatra Shrabani Pradhan Koushik Das Dilip Kumar Nandi Department of Nutrition Microbiology and Human PhysiologyRaja N L Khan Women's College

Objective:To investigate phytochemical screening,antimicrobial activity and qualitative thin layer chromatographic separation of flavonoid components,antioxidant activity and total flavonoid compound of Terminalia arjuna.Methods:For phytochemical screening,some common and available standard tests were done.Antimicrobial bioassay was done through agar well diffusion method.Detection of antioxidant activity and flavonoid compounds were done through thin layer chromatography.Total antioxidant activity was measured by 2,2-diphenyl-1-picrylhydrazyl(DPPH)in colorimetric method.Aluminum chloride colorimetric method was used for total flavonoid determination.Results:Phytochemical screening showed the active compounds presence in high concentration,such as phytosterol,lactones,flavonoids,phenolic compounds and tannins and glycosides.The anlimicrobial activity of extract showed that greater inhibition zone against Gram negative bacteria than Gram positive bacteria.This methanolic extract showed a promising antioxidant activity,as absorption of DPPH redicles decreased in DPPH free radical scavenging assay.Flavonoids components having antioxidant property present in the methanol extract at a level of199.00 mg quercetin equivalent/g of dried methanol extract in colorimetric method.Conclusions:The Terminalia arjuna bark extract revealed the presence of bio-active constituents which are known to exhibit medicinal as well as physiological activities.

关键词： Terminalia arjuna Phytocompound tlc Antioxidant Total flavonoid Antimicrobial activity DPPH

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坚杆火绒草质量标准的研究

中成药 2022年第2期44卷 380-384页

作者：赵雪莲李奕松苏宏娜谭玲李文兵杨正明刘圆西南民族大学药学院四川成都610041 四川省羌彝药用资源保护与利用技术工程实验室四川成都610225 青藏高原民族药用资源保护与利用国家民委重点实验室四川成都610225 西南民族大学青藏高原研究院四川成都610041 西南民族大学民族医药研究院四川成都610041

目的建立坚杆火绒草质量标准。方法显微法、tlc法进行定性鉴别,2020年版《中国药典》方法测定水分、总灰分、酸不溶性灰分、浸出物含量,HPLC法测定绿原酸、3,5-O-二咖啡酰基奎宁酸含量。结果显微特征明显,可见表皮细胞、气孔、非腺毛、... 详细信息

目的建立坚杆火绒草质量标准。方法显微法、tlc法进行定性鉴别,2020年版《中国药典》方法测定水分、总灰分、酸不溶性灰分、浸出物含量,HPLC法测定绿原酸、3,5-O-二咖啡酰基奎宁酸含量。结果显微特征明显,可见表皮细胞、气孔、非腺毛、梯纹导管、螺纹导管、木纤维、薄壁细胞、花粉粒等。tlc斑点清晰,分离度好。6批样品水分、总灰分、酸不溶性灰分、浸出物含量分别为0.92%~2.87%、7.28%~11.90%、1.08%~5.28%、20.38%~23.02%,绿原酸、3,5-O-二咖啡酰基奎宁酸在各自范围内线性关系良好(R^(2)=0.9999),平均加样回收率分别为99.22%、98.96%,RSD分别为1.51%、1.46%。结论该方法合理可行,可用于坚杆火绒草的质量控制。

关键词：坚杆火绒草绿原酸 3,5-O-二咖啡酰基奎宁酸显微法 tlc HPLC

Cytotoxicity evaluation and hepatoprotective potential of bioassay guided fractions from Feronia limmonia Linn leaf

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Asian Pacific Journal of Tropical Biomedicine 2011年第6期1卷 443-447页

作者： Mahendra Jain Rakhee Kapadia Ravirajsinh N Jadeja Menaka C Thounaojam Ranjitsinh V Devkar SH Mishra Herbal Drug Technology Laboratory Pharmacy DepartmentFaculty of Technology and EngineeringThe M.S.University of Baroda Novel Drug Delivery System Laboratory Pharmacy DepartmentFaculty of Technology and EngineeringThe M.S.University of Baroda Division of Phytothrapeutics and Metabolic Endocrinology Department of ZoologyThe M.S.University of Baroda

Objective:To evaluate the cytotoxicity and hepatoprotective potentials of extracts,fractions or isolated compound from the leaves of Feronia limonia(F.limonia).Methods:Qualitative phytochemical analysis of extracts,fractions or compound was performed by means of thin layer chromatography and spectroscopic assays.The%purity of compound was measured by analytical HPLC.Extracts,fractions or compound have been individually evaluated for their cytotoxicity effects(10,20,100,250,500,750 and 1 000 μg/mL).Based on the inhibitory concentration(IC_(50)) obtained from the cell viability assay,graded concentrations of extracts,fractions or isolated compound were assessed(10,20,50,100,200 μg/mL) for its hepatoprotective potential against CCl_4-induced hepatotoxicity by monitoring activity levels of serum glutamatic pyruvatic transaminase(SGPT) and serum glutamic oxaloacetic transaminase(SGOT).Results:Results indicated that the methanol extract of F.limonia was non-toxic and hepatoprotective in nature as compared with the petroleum ether extract.The acetone fraction of methanolic extract also showed similar properties but the subsequent two fractions were cytotoxic.However,the pure compound isolated from the penultimate fraction of methanolic extract was non-toxic and hepatoprotective in nature.Biochemical investigations(SCOT,SCPT) further corroborated these cytological observations.Conclusions:It can be concluded from this study that F.limonia methanol extract,some fractions and pure isolated compound herein exhibit hepatoprotective activity.However,cytotoxicity recorded in the penultimate fraction and investigation of structural details of pure compound warrants further study.

关键词： Feronia limonia leaf HepG2 Cytotoxicity Hepatoprotective Phytochemical analysis tlc HPLC Hepatoprotective activity SGPT SGOT Isolated compound Spectroscopic assay

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脑心清片质量标准提升

中成药 2023年第12期45卷 3910-3913页

作者：刘傲雪冯文明张甦鉴甄检测技术(上海)有限公司上海200131

目的提升脑心清片质量标准。方法tlc法定性鉴别山柰酚,建立特征图谱,UPLC法测定金丝桃苷、异槲皮苷、紫云英苷、槲皮素、山柰酚含量。结果tlc斑点清晰,分离度良好。15批样品特征图谱中有12个共有峰,相对保留时间均在规定值的±5%以内。... 详细信息

目的提升脑心清片质量标准。方法tlc法定性鉴别山柰酚,建立特征图谱,UPLC法测定金丝桃苷、异槲皮苷、紫云英苷、槲皮素、山柰酚含量。结果tlc斑点清晰,分离度良好。15批样品特征图谱中有12个共有峰,相对保留时间均在规定值的±5%以内。5种黄酮在各自范围内线性关系良好(r=1.0000),平均加样回收率95.38%~105.16%,RSD 0.73%~2.31%。结论该方法专属性强,重复性、耐用性良好,可实现对脑心清片有效成分黄酮的全面质量控制。

关键词：脑心清片黄酮特征图谱 tlc UPLC