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转tcs基因山新杨的种植对土壤微生物生态系统的影响

转TCS基因山新杨的种植对土壤微生物生态系统的影响

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作者：崔嵘东北林业大学

学位级别：硕士

The species of Populus *** important trees planted worldwide,and play crucial roles in forest industry in our *** present,the application of transgenic biotechnology in poplar genetic breeding has accelerated the selection process of its fine new ***,the problem of transgenic ecological safety caused by exogenous gene has become the focus of current ***,it is of great significance to evaluate the ecological safety of transgenic trees in theory research and production *** study to the tcs gene Populus davidiana×*** of radix trichosanthis protein fusion protein gene and to study its genetic traits after change of rhizosphere secretion influence on soil microorganisms and soil physical and chemical properties,in order to evaluate the release of transgenic plants and its gene product may cause risk of soil ecological environment to provide technical *** main results are as follows:(1)This study has found that tcs gene Populus davidiana×*** have significant effects on five kinds of soil enzyme activities(Ptcs gene.(2)This study has found that the tcs gene Populus davidiana×Pbolleana plant after 30d-210d period compared with the control soil,the soil turned Populus davidiana×*** root secretion of tcs gene mountain index with different degree of impact of soil physical and chemical properties,in addition to the content of soil organic matter in part time difference was significant(Ptcs gene mountain new poplar secretions,which has a great influence on some time periods.(3)In this study,five kinds of soil enzyme activities and 6 soil physical and chemical properties indexes were analyzed,and most of the indexes were related to each other,and some of them reached significant or significant levels

关键词： tcs gene Populus davidiana×P.bolleana Soil microorganisms Soil enzyme activity Physical and chemical properties of soil Soil microbial population Soil microbial functional diversity

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