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^(99m)tc-methylene diphosphonate uptake at injury site correlates with osteoblast differentiation and mineralization during bone healing in mice

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bone Research 2015年第2期3卷 92-99页

作者： Zhendong A Zhong Anderson Peck Shihong Li Jeff Van Oss John Snider Casey J Droscha Tingtung A Chang Bart O Williams Center for Skeletal Disease Research Van Andel Research Institute Department of Internal Medicine Center for Musculoskeletal Health UC Davis Medical Center Small Animal Imaging Facility Van Andel Research Institute

99mtc-methylene diphosphonate （99mtc-MDP） is widely used in clinical settings to detect bone abnormalities. However, the mechanism of 99mtc-MDP uptake in bone is not well elucidated. In this study, we utilized a mouse tibia injury model, single-photon emission computed tomography （gamma scintigraphy or SPECT）, ex vivo micro-computed tomography, and histology to monitor 99mtc-MDP uptake in injury sites during skeletal healing. In an ex vivo culture system, calvarial cells were differentiated into osteoblasts with osteogenic medium, pulsed with 99mtc-MDP at different time points, and quantitated for 99mtc-MDP uptake with a gamma counter. We demonstrated that 99mtc-MDP uptake in the injury sites corresponded to osteoblast generation in those sites throughout the healing process. The 99mtc-MDP uptake within the injury sites peaked on day 7 post-injury, while the injury sites were occupied by mature osteoblasts also starting from day 7. ~mtc-MDP uptake started to decrease 14 days post-surgery, when we observed the highest level of bony tissue in the injury sites. We also found that 99mtc-MDP uptake was associated with osteoblast maturation and mineralization in vitro. This study provides direct and biological evidence for 99mtc-MDP uptake in osteoblasts during bone healing in vivo and in vitro.

关键词： MDP bone tc-methylene diphosphonate uptake at injury site correlates with osteoblast differentiation and mineralization during bone healing in mice SPECT

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