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Mechanism underlying berberine's effects on HSP70/TNFα under heat stress:Correlation with the tata boxes

Chinese Journal of Natural Medicines 2017年第3期15卷 178-191页

作者： JIANG Jing-Fei LEI Fan YUAN Zhi-Yi WANG Yu-Gang WANG Xin-Pei YAN Xiao-Jin YU Xuan XING Dong-Ming DU Li-Jun MOE(Ministry of Education)Key Laboratory of Protein Sciences Laboratory of Molecular Pharmacology and Pharmaceutical SciencesSchool of Life SciencesTsinghua UniversityBeijing 100084China Department of Pharmacology and Pharmaceutical Sciences School of MedicineTsinghua UniversityBeijing 100084China MD Anderson Cancer Center University of TexasHoustonTX 77030USA

Heat stress can stimulate an increase in body temperature,which is correlated with increased expression of heat shock protein 70(HSP70) and tumor necrosis factor α(TNFα).The exact mechanism underlying the HSP70 and TNFα induction is unclear.Berberine(BBR) can significantly inhibit the temperature rise caused by heat stress,but the mechanism responsible for the BBR effect on HSP70 and TNFα signaling has not been investigated.The aim of the present study was to explore the relationship between the expression of HSP70 and TNFα and the effects of BBR under heat conditions,using in vivo and in vitro models.The expression levels of HSP70 and TNFα were determined using RT-PCR and Western blotting analyses.The results showed that the levels of HSP70 and TNFα were up-regulated under heat conditions(40 °C).HSP70 acted as a chaperone to maintain TNFα homeostasis with rising the temperature,but knockdown of HSP70 could not down-regulate the level of TNFα.Furthermore,TNFα could not influence the expression of HSP70 under normal and heat conditions.BBR targeted both HSP70 and TNFα by suppressing their gene transcription,thereby decreasing body temperature under heat conditions.In conclusion,BBR has a potential to be developed as a therapeutic strategy for suppressing the thermal effects in hot environments.

关键词： Berberine Hyperthermia HSP70 TNFα tata box

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MicroRNA Primary Transcripts and Promoter Elements Analysis in Soybean (Glycine max L. Merril.)

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Journal of Integrative Agriculture 2013年第9期12卷 1522-1529页

作者： LI Jing LIU Yong-xin HAN Ying-peng LI Yong-guang GUO Mao-zu LI Wen-bin Key Laboratory of Soybean Biology Ministry of Education/Northeast Agricultural University Department of Computer Science and Technology Harbin Institute of Technology

The importance of microRNA （miRNA） at the post-transcriptional regulation level has recently been recognized in both animals and plants. In recent years, many studies focused on miRNA target identification and functional analysis. However, little is known about the transcription and regulation of miRNAs themselves. In this study, the transcription start sites （TSSs） for 11 miRNA primary transcripts of soybean from 11 miRNA loci （of 50 loci tested） were cloned by a 5＂ rapid amplification of cDNA ends （5＂ RACE） procedure using total RNA from 30-d-old seedlings. The features consistent with a RNA polymerase II mechanism of transcription were found among these miRNA loci. A position weight matrix algorithm was used to identify conserved motifs in miRNA core promoter regions. A canonical tata box motif was identified upstream of the major start site at 8 （76%） of the mapped miRNA loci. Several cis-acting elements were predicted in the 2 kb 5＂ to the TSSs. Potential spatial and temporal expression patterns of the miRNAs were found. The target genes for these miRNAs were also predicted and further elucidated for the potential function of the miRNAs. This research provides a molecular basis to explore regulatory mechanisms of miRNA expression, and a way to understand miRNA-mediated regulatory pathways and networks in soybean.

关键词： soybean miRNA primary transcript RACE tata box motif promoter cis-acting element

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Myostatin gene promoter:structure,conservation and importance as a target for muscle modulation

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Journal of Animal Science and Biotechnology 2019年第2期10卷 255-273页

作者： Carla Vermeulen Carvalho Grade Carolina Stefano Mantovani Lúcia Elvira Alvares Universidade Federal da Integracao Latino-Americana UNILAInstituto Latino-Americano de Ciências da Vida e da NaturezaAvenida Tarquínio Joslin dos Santos1000Foz do IguacuPR CEP 85870-901Brazil Departamento de Bioquímica e Biologia Tecidual Universidade Estadual de Campinas-UNICAMPRua Monteiro Lobato255CampinasSP CEP 13083-862Brazil.

Myostatin(MSTN) is one of the key factors regulating myogenesis. Because of its role as a negative regulator of muscle mass deposition, much interest has been given to its protein and, in recent years, several studies have analysed MSTN gene regulation. This review discusses the MSTN gene promoter, focusing on its structure in several animal species, both vertebrate and invertebrate. We report the important binding sites considering their degree of phylogenetic conservation and roles they play in the promoter activity. Finally, we discuss recent studies focusing on MSTN gene regulation via promoter manipulation and the potential applications they have both in medicine and agriculture.

关键词： CAAT box E-box Gene promoter Myogenesis Myostatin SNP Transcription factors tata box

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美洲黑杨材性候选基因PdZFR的克隆和分析

美洲黑杨材性候选基因PdZFR的克隆和分析

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第二届中国林业学术大会——S2功能基因组时代的林木遗...

作者：王大海苏晓华张冰玉黄秦军张香华中国林业科学研究院林业研究所

根据美洲黑杨PdZFR部分cDNA序列采用RACE和拟基因组文库步行法克隆了该基因全长cDNA和基因组上全长片段。该基因开放编码框由615个碱基组成,编码205个氨基酸组成的多肽,其等电点为6.94,MW为24.37kD。通过Blastx软件对GenBank的nr数据库... 详细信息

根据美洲黑杨PdZFR部分cDNA序列采用RACE和拟基因组文库步行法克隆了该基因全长cDNA和基因组上全长片段。该基因开放编码框由615个碱基组成,编码205个氨基酸组成的多肽,其等电点为6.94,MW为24.37kD。通过Blastx软件对GenBank的nr数据库进行比对,该基因与拟南芥C3HC4-type ZF-RING同源性达86%。对PdZFR基因转录、剪切分析表明,该基因包含9个内含子和10个外显子,剪切出的内含子两端趋于保守,为’AG’和’GT’,外显子平均长度较内含子短,属于I类内含子。该基因转录起始碱基为G(+1,1817),无明显真核生物启动子核心序列tata框,从+1(1817)到+13(1830)区域A/T含量明显较高。该基因转录终止位置,存在大量重复序列,有利于在转录过程中形成局部二级结构,调节基因转录终止。基因组上分布分析表明,该基因位于杨树基因组LGI上对应物理位置的16611332-16616623之间,并在杨树基因组LGVIII上有部分同源序列。各部位表达分析表明,该基因与植物发育和次生生长有关。

关键词： PdZFR 美洲黑杨 RACE VGL-Walking tata box CAAT box

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